Restriction map for
pFN21AB5883
(Circular) (Six-base) MAPSORT of: pFN21AB5883.seq Check: 5403 from: 1 to: 6106
pFN21AB5883 6106 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 4169 5553 5821 1632
Size: 455 2082 1384 268 1917
Fragments arranged by size:
2082 1917 1384 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 2342 4835 2342
Size: 2493 3613
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 6074
AlwNI CAG_nnn'CTG
Cuts at: 2833 3079 3849 5421 2833
Size: 246 770 1572 3518
Fragments arranged by size:
3518 1572 770 246
ApaI G_GGCC'C
Cuts at: 2025 2308 2725 3068 2025
Size: 283 417 343 5063
Fragments arranged by size:
5063 417 343 283
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 2357 3397 3415 3641 3793 5902 1493
Size: 864 1040 18 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 864 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2324 3431 1870
Size: 73 381 1107 4545
Fragments arranged by size:
4545 1107 381 73
AvrII C'CTAG_G
Cuts at: 2623 2623
Size: 6106
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3427 4882 618
Size: 325 217 627 1640 1455 1842
Fragments arranged by size:
1842 1640 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2308 2725 2937 3068 3425 1391
Size: 634 283 417 212 131 357 4072
Fragments arranged by size:
4072 634 417 357 283 212 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2447 3998 5208 2447
Size: 1551 1210 3345
Fragments arranged by size:
3345 1551 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2230 2853 4717
Size: 107 122 71 631 605 558 623 1864
Cuts at: 4717 136
Size: 1525
Fragments arranged by size:
1864 1525 631 623 605 558 122 107
71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2157 2308 2622 2725 2801 3068 4167
Size: 235 132 151 314 103 76 267 1099
Cuts at: 4167 5323 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1099 314 267 235 151 132
103 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2728 2763 3837
Size: 416 53 83 399 24 1311 35 1074
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1311 1074 958 416 399 83 53
35 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2265 2351 3077 4766 1239
Size: 1026 86 726 1689 2579
Fragments arranged by size:
2579 1689 1026 726 86
Bpu10I CC'TnA_GC
Cuts at: 3186 3186
Size: 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3140 4231 5096 5394 3140
Size: 1091 865 298 3852
Fragments arranged by size:
3852 1091 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2458 3304 4769 882
Size: 617 959 846 1465 2219
Fragments arranged by size:
2219 1465 959 846 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2779 4539 4937 5211 5358 5769 2779
Size: 1760 398 274 147 411 3116
Fragments arranged by size:
3116 1760 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5881
Fragments arranged by size:
5881 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2358 2987 3004 3013 1186
Size: 1172 629 17 9 4279
Fragments arranged by size:
4279 1172 629 17 9
BseYI C'CCAG_C
Cuts at: 2079 2209 2233 2397 5309 5805 2079
Size: 130 24 164 2912 496 2380
Fragments arranged by size:
2912 2380 496 164 130 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2663 3114 1163
Size: 573 927 451 4155
Fragments arranged by size:
4155 927 573 451
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 3265 4167 4252 5323 2703
Size: 562 902 85 1071 3486
Fragments arranged by size:
3486 1071 902 562 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2157 2308 2622 2703 2725 2801
Size: 399 235 132 151 314 81 22 76
Cuts at: 2801 2937 3068 3265 3425 4167 4252 5323 1391
Size: 136 131 197 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 314 235 197 160
151 136 132 131 85 81 76 22
BspEI T'CCGG_A
Cuts at: 2779 5769 2779
Size: 2990 3116
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2991 4601 4775 65
Size: 1183 1743 1610 174 1396
Fragments arranged by size:
1743 1610 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2174 794
Size: 1380 4726
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 3295 3295
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2551 3106 3436 3803 4188 4205 5657 5743
Size: 579 555 330 367 385 17 1452 86
Cuts at: 5743 5755 6101 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 579 555 385 367 346 330
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 513
Size: 552 939 12 4603
Fragments arranged by size:
4603 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 3622 4416 4436 3622
Size: 794 20 5292
Fragments arranged by size:
5292 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2910 3553
Size: 54 1425 50 275 18 225 855 643
Cuts at: 3553 4442 6071 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 643 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2185 2233 2415 3167 4036 1353
Size: 832 48 182 752 869 3423
Fragments arranged by size:
3423 869 832 752 182 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2265 2351 3077 4169 4766 5553
Size: 393 455 178 86 726 1092 597 787
Cuts at: 5553 5821 1239
Size: 268 1524
Fragments arranged by size:
1524 1092 787 726 597 455 393 268
178 86
EcoO109I rG'GnC_Cy
Cuts at: 2021 2071 2104 2222 2266 2344 2709 2722 2895
Size: 50 33 118 44 78 365 13 173
Cuts at: 2895 2963 3065 2021
Size: 68 102 5062
Fragments arranged by size:
5062 365 173 118 102 78 68 50
44 33 13
EcoRI G'AATT_C
Cuts at: 2357 3397 3415 3793 2357
Size: 1040 18 378 4670
Fragments arranged by size:
4670 1040 378 18
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 5253 1678
Size: 3575 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2328 2360 2536 2568 3052
Size: 32 135 32 756 32 176 32 484
Cuts at: 3052 3084 4747 4779 4821 4853 1373
Size: 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 756 484 176 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3100 3450 3696 669
Size: 988 281 1162 350 246 3079
Fragments arranged by size:
3079 1162 988 350 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2910 3246 5220 5404 1305
Size: 1605 336 1974 184 2007
Fragments arranged by size:
2007 1974 1605 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2912 10
Size: 54 1425 343 225 855 3204
Fragments arranged by size:
3204 1425 855 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2794 2814 4064 4423 4582 518
Size: 648 1628 20 1250 359 159 2042
Fragments arranged by size:
2042 1628 1250 648 359 159 20
MspA1I CmG'CkG
Cuts at: 1606 3522 4199 5347 5592 1606
Size: 1916 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4615
Fragments arranged by size:
4615 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2177 3464 5009 1132
Size: 97 948 1287 1545 2229
Fragments arranged by size:
2229 1545 1287 948 97
PasI CC'CwG_GG
Cuts at: 1348 3197 1348
Size: 1849 4257
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2262 2753 3197 1108
Size: 1154 491 444 4017
Fragments arranged by size:
4017 1154 491 444
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2266 2344 2895 2963 2104
Size: 162 78 551 68 5247
Fragments arranged by size:
5247 551 162 78 68
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 2021 2304 2721 3064 2021
Size: 283 417 343 5063
Fragments arranged by size:
5063 417 343 283
PstI C_TGCA'G
Cuts at: 830 2051 3458 830
Size: 1221 1407 3478
Fragments arranged by size:
3478 1407 1221
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 2233 2233
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2047 2448 3270 3454 4589 5270 5461
Size: 219 1002 401 822 184 1135 681 191
Cuts at: 5461 826
Size: 1471
Fragments arranged by size:
1471 1135 1002 822 681 401 219 191
184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3119 4210 5111 5373 820
Size: 197 926 1176 1091 901 262 1553
Fragments arranged by size:
1553 1176 1091 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2177 3464 1132
Size: 1045 1287 3774
Fragments arranged by size:
3774 1287 1045
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2004 2099 2623 3288 3545 513
Size: 552 939 95 524 665 257 3074
Fragments arranged by size:
3074 939 665 552 524 257 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4352 6064 96
Size: 274 80 33 51 1443 2375 1712 138
Fragments arranged by size:
2375 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2510 2326
Size: 184 5922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI