Restriction map for
pFN21AB5880
(Circular) (Six-base) MAPSORT of: pFN21AB5880.seq Check: 4894 from: 1 to: 6193
pFN21AB5880 6193 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 6193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 3276 4320 4693 1563
Size: 1713 1044 373 3063
Fragments arranged by size:
3063 1713 1044 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4256 5640 5908 1632
Size: 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2562 5092 6069 1203
Size: 1359 2530 977 1327
Fragments arranged by size:
2530 1359 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 3158 1166
Size: 1992 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 6161
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 3050 4250 5406 3050
Size: 1200 1156 3837
Fragments arranged by size:
3837 1200 1156
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 2268 3523 3890 2268
Size: 1255 367 4571
Fragments arranged by size:
4571 1255 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2879 3514 4969 618
Size: 325 217 627 254 838 635 1455 1842
Fragments arranged by size:
1842 1455 838 635 627 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2451 2605 3512 1391
Size: 1060 154 907 4072
Fragments arranged by size:
4072 1060 907 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2359 4085 5295 2359
Size: 1726 1210 3257
Fragments arranged by size:
3257 1726 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 3054 4254 5410 1790
Size: 1264 1200 1156 2573
Fragments arranged by size:
2573 1264 1200 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3924 4882
Size: 416 53 83 399 24 1206 1301 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1301 1206 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2343 1056
Size: 1287 4906
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2799 2317
Size: 482 5711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2769 2843 3410 4853 1239
Size: 942 588 74 567 1443 2579
Fragments arranged by size:
2579 1443 942 588 567 74
Bpu10I CC'TnA_GC
Cuts at: 2945 3197 2945
Size: 252 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 3383 4318 5183 5481 2641
Size: 742 935 865 298 3353
Fragments arranged by size:
3353 935 865 742 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2659 4856 882
Size: 617 1160 2197 2219
Fragments arranged by size:
2219 2197 1160 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3315 4000 4382 5017
Size: 53 83 186 936 1782 685 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1782 1451 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3110 3140 1178
Size: 30 165 30 1707 30 4231
Fragments arranged by size:
4231 1707 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2348 3050 3125 1186
Size: 940 222 702 75 4254
Fragments arranged by size:
4254 940 702 222 75
BseYI C'CCAG_C
Cuts at: 2386 2692 3011 3455 5396 5892 2386
Size: 306 319 444 1941 496 2687
Fragments arranged by size:
2687 1941 496 444 319 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5620
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2500 3643 4404 5432 1540
Size: 275 186 499 1143 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 499 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 3054 3123 4254 4339 5410 2091
Size: 963 69 1131 85 1071 2874
Fragments arranged by size:
2874 1131 1071 963 85 69
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2451 2605 3054 3123 3512 4254
Size: 399 301 360 154 449 69 389 742
Cuts at: 4254 4339 5410 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 389 360 301
154 85 69
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 3099 3225 3306 4082 5812 3099
Size: 126 81 776 1730 3480
Fragments arranged by size:
3480 1730 776 126 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 2877 2891 3432 3548 844
Size: 1482 551 14 541 116 3489
Fragments arranged by size:
3489 1482 551 541 116 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 4837 5024 1700
Size: 110 228 2799 187 2869
Fragments arranged by size:
2869 2799 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 2520 96
Size: 2424 3769
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2100 794
Size: 1306 4887
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2619 2329
Size: 290 5903
BstYI r'GATC_y
Cuts at: 1972 2268 3364 3523 3890 4275 4292 5744 5830
Size: 296 1096 159 367 385 17 1452 86
Cuts at: 5830 5842 6188 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1096 385 367 346 296 159
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2328 2407 2415 2912 513
Size: 552 939 324 79 8 497 3794
Fragments arranged by size:
3794 939 552 497 324 79 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2034 2089 2215 2847 3709 4503 4523 2034
Size: 55 126 632 862 794 20 3704
Fragments arranged by size:
3704 862 794 632 126 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2116 2151 2901 2936 529
Size: 35 1552 35 750 35 3786
Fragments arranged by size:
3786 1552 750 35 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3320 5200 809
Size: 507 2004 1880 1802
Fragments arranged by size:
2004 1880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2609 3640
Size: 54 1425 50 275 18 250 529 1031
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1031 889 529 275 250 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3300 4123 1353
Size: 1947 823 3423
Fragments arranged by size:
3423 1947 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2429 4780 5154 5300 387
Size: 1064 138 435 405 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 435 405 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2769 2843 3410 4256 4853 5640
Size: 393 549 588 74 567 846 597 787
Cuts at: 5640 5908 1239
Size: 268 1524
Fragments arranged by size:
1524 846 787 597 588 567 549 393
268 74
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 6193
EcoO109I rG'GnC_Cy
Cuts at: 2315 2315
Size: 6193
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 2688 5340 1678
Size: 1010 2652 2531
Fragments arranged by size:
2652 2531 1010
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373
Size: 32 135 32 3262 32 42 32 2626
Fragments arranged by size:
3262 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3442 3792 3442
Size: 350 5843
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2642 3260 3457 5307 5491 1305
Size: 1337 618 197 1850 184 2007
Fragments arranged by size:
2007 1850 1337 618 197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2709 3158 3224 4151 4510 4669 518
Size: 648 1543 449 66 927 359 159 2042
Fragments arranged by size:
2042 1543 927 648 449 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2050 3011 3609 4286 5434 5679 1606
Size: 444 961 598 677 1148 245 2120
Fragments arranged by size:
2120 1148 961 677 598 444 245
NaeI GCC'GGC
Cuts at: 2040 2040
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 2004 2328 2407 513
Size: 552 939 324 79 4299
Fragments arranged by size:
4299 939 552 324 79
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 2038 2038
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 2339 1052
Size: 1287 4906
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2566 3551 5096 1132
Size: 97 1337 985 1545 2229
Fragments arranged by size:
2229 1545 1337 985 97
PasI CC'CwG_GG
Cuts at: 1348 2464 1348
Size: 1116 5077
PciI A'CATG_T
Cuts at: 2562 5092 2562
Size: 2530 3663
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PfoI T'CCnGG_A
Cuts at: 2271 3387 2271
Size: 1116 5077
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 4262 4294 5775 5807 3110
Size: 32 1120 32 1481 32 3496
Fragments arranged by size:
3496 1481 1120 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 2315
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PstI C_TGCA'G
Cuts at: 830 2096 2888 3429 3545 830
Size: 1266 792 541 116 3478
Fragments arranged by size:
3478 1266 792 541 116
PvuI CG_AT'CG
Cuts at: 2001 2500 2001
Size: 499 5694
PvuII CAG'CTG
Cuts at: 3011 3011
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 2888 3545 2888
Size: 657 5536
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SexAI A'CCwGG_T
Cuts at: 3065 3065
Size: 6193
SfcI C'TryA_G
Cuts at: 826 1045 2092 2884 3425 3541 4676 5357 5548
Size: 219 1047 792 541 116 1135 681 191
Cuts at: 5548 826
Size: 1471
Fragments arranged by size:
1471 1135 1047 792 681 541 219 191
116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2656 3398 4297 5198 5460 820
Size: 197 926 713 742 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 742 713 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2328 2407 3632 513
Size: 552 939 324 79 1225 3074
Fragments arranged by size:
3074 1225 939 552 324 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 2889 3299 4220 4237 4390 4575
Size: 1255 840 469 410 921 17 153 185
Cuts at: 4575 5020 325
Size: 445 1498
Fragments arranged by size:
1498 1255 921 840 469 445 410 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 4439 6151
Size: 274 80 33 51 1443 543 1919 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1919 1712 1443 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3298 3529 3298
Size: 231 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2636 2636
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI