Restriction map for
pFN21AB5877
(Circular) (Six-base) MAPSORT of: pFN21AB5877.seq Check: 6624 from: 1 to: 6100
pFN21AB5877 6100 bp
With 182 enzymes: SgfI *
March 28, 2012 12:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 3443 1937
Size: 1078 428 4594
Fragments arranged by size:
4594 1078 428
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4163 5547 5815 1632
Size: 2531 1384 268 1917
Fragments arranged by size:
2531 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 2418 4999 5976 1203
Size: 1215 2581 977 1327
Fragments arranged by size:
2581 1327 1215 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 2507 1166
Size: 1341 4759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 3843 5415 3843
Size: 1572 4528
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3331 3409 3635 3787 5896 1493
Size: 1838 78 226 152 2109 1697
Fragments arranged by size:
2109 1838 1697 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 1870
Size: 73 1482 4545
Fragments arranged by size:
4545 1482 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 3421 4876 618
Size: 325 217 627 678 956 1455 1842
Fragments arranged by size:
1842 1455 956 678 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3047 3165 3419 1391
Size: 1656 118 254 4072
Fragments arranged by size:
4072 1656 254 118
BbeI G_GCGC'C
Cuts at: 2469 2469
Size: 6100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2108 2251
Size: 107 122 71 631 605 427 9 143
Cuts at: 2251 2405 4711 136
Size: 154 2306 1525
Fragments arranged by size:
2306 1525 631 605 427 154 143 122
107 71 9
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 4161 5317 1790
Size: 2371 1156 2573
Fragments arranged by size:
2573 2371 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 3831 4789
Size: 416 53 83 399 24 713 1701 958
Cuts at: 4789 442
Size: 1753
Fragments arranged by size:
1753 1701 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2090 3027 1056
Size: 1034 937 4129
Fragments arranged by size:
4129 1034 937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4760 1239
Size: 3521 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3149 4225 5090 5388 3149
Size: 1076 865 298 3861
Fragments arranged by size:
3861 1076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3294 4763 882
Size: 617 1795 1469 2219
Fragments arranged by size:
2219 1795 1469 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2397 2466 3907
Size: 53 83 186 936 531 333 69 1441
Cuts at: 3907 4289 4924 275
Size: 382 635 1451
Fragments arranged by size:
1451 1441 936 635 531 382 333 186
83 69 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 1178
Size: 30 165 30 741 30 5104
Fragments arranged by size:
5104 741 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2149 2152 2847 1186
Size: 963 3 695 4439
Fragments arranged by size:
4439 963 695 3
BseYI C'CCAG_C
Cuts at: 2017 2176 2208 5303 5799 2017
Size: 159 32 3095 496 2318
Fragments arranged by size:
3095 2318 496 159 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2261 1163
Size: 573 343 182 5002
Fragments arranged by size:
5002 573 343 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3047 3165 4161 4246 5317 3047
Size: 118 996 85 1071 3830
Fragments arranged by size:
3830 1071 996 118 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3047 3165 3419 4161 4246 5317 1391
Size: 399 1257 118 254 742 85 1071 2174
Fragments arranged by size:
2174 1257 1071 742 399 254 118 85
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 2866 2919 3989 5719 2866
Size: 53 1070 1730 3247
Fragments arranged by size:
3247 1730 1070 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3001 3455 844
Size: 2157 454 3489
Fragments arranged by size:
3489 2157 454
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 2717 2780 3045 4595 4769 65
Size: 1183 1290 179 63 265 1550 174 1396
Fragments arranged by size:
1550 1396 1290 1183 265 179 174 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 3189 4744 4931 1700
Size: 110 1379 1555 187 2869
Fragments arranged by size:
2869 1555 1379 187 110
BsrGI T'GTAC_A
Cuts at: 96 3054 3204 96
Size: 2958 150 2992
Fragments arranged by size:
2992 2958 150
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095
Size: 1458 367 385 17 1452 86 12 346
Cuts at: 6095 1972
Size: 1977
Fragments arranged by size:
1977 1458 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2403 513
Size: 552 939 99 300 4210
Fragments arranged by size:
4210 939 552 300 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2242 487
Size: 683 56 1016 4345
Fragments arranged by size:
4345 1016 683 56
BtsI GCAGTG_nn'
Cuts at: 2301 2967 3616 4410 4430 2301
Size: 666 649 794 20 3971
Fragments arranged by size:
3971 794 666 649 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2100 2406
Size: 54 1425 50 275 18 177 93 306
Cuts at: 2406 2535 2714 3547 4436 6065 8
Size: 129 179 833 889 1629 43
Fragments arranged by size:
1629 1425 889 833 306 275 179 177
129 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4030 1353
Size: 2677 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3337 4687 5061 5207 387
Size: 1064 138 1748 1350 374 146 1280
Fragments arranged by size:
1748 1350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3045 3163 3045
Size: 118 5982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4163 4760 5547 5815 1239
Size: 393 2531 597 787 268 1524
Fragments arranged by size:
2531 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2723 2723
Size: 6100
EcoRI G'AATT_C
Cuts at: 3331 3409 3787 3331
Size: 78 378 5644
Fragments arranged by size:
5644 378 78
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2469 5247 1678
Size: 791 2778 2531
Fragments arranged by size:
2778 2531 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2823 2855 4741 4773 4815
Size: 32 135 32 1251 32 1886 32 42
Cuts at: 4815 4847 1373
Size: 32 2626
Fragments arranged by size:
2626 1886 1251 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KasI G'GCGC_C
Cuts at: 2465 2465
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 2794 3257 5214 5398 1305
Size: 1113 376 463 1957 184 2007
Fragments arranged by size:
2007 1957 1113 463 376 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2716 10
Size: 54 1425 343 177 707 3394
Fragments arranged by size:
3394 1425 707 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 2543 4058 4417 4576 518
Size: 648 1341 36 1515 359 159 2042
Fragments arranged by size:
2042 1515 1341 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2507 3516 4193 5341 5586 1606
Size: 901 1009 677 1148 245 2120
Fragments arranged by size:
2120 1148 1009 901 677 245
NarI GG'CG_CC
Cuts at: 2466 2466
Size: 6100
NcoI C'CATG_G
Cuts at: 513 1065 2004 2403 513
Size: 552 939 399 4210
Fragments arranged by size:
4210 939 552 399
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 2086 3023 1052
Size: 1034 937 4129
Fragments arranged by size:
4129 1034 937
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3458 5003 1132
Size: 97 2229 1545 2229
Fragments arranged by size:
2229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2214 1348
Size: 866 5234
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2723 2723
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PstI C_TGCA'G
Cuts at: 830 3452 830
Size: 2622 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2507 2507
Size: 6100
SacI G_AGCT'C
Cuts at: 3047 3165 3047
Size: 118 5982
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SexAI A'CCwGG_T
Cuts at: 3173 3266 3173
Size: 93 6007
SfcI C'TryA_G
Cuts at: 826 1045 2595 3448 4583 5264 5455 826
Size: 219 1550 853 1135 681 191 1471
Fragments arranged by size:
1550 1471 1135 853 681 219 191
SfoI GGC'GCC
Cuts at: 2467 2467
Size: 6100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 4204 5105 5367 820
Size: 197 926 1221 1040 901 262 1553
Fragments arranged by size:
1553 1221 1040 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 3395 4024 5
Size: 47 3343 629 2081
Fragments arranged by size:
3343 2081 629 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2403 3539 513
Size: 552 939 399 1136 3074
Fragments arranged by size:
3074 1136 939 552 399
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2112 4127 4144 4297 4482 4927
Size: 1255 465 67 2015 17 153 185 445
Cuts at: 4927 325
Size: 1498
Fragments arranged by size:
2015 1498 1255 465 445 185 153 67
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 3054 3204
Size: 274 80 33 51 1443 792 285 150
Cuts at: 3204 4346 6058 96
Size: 1142 1712 138
Fragments arranged by size:
1712 1443 1142 792 285 274 150 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2172 2204 6060 6092 2172
Size: 32 3856 32 2180
Fragments arranged by size:
3856 2180 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SrfI SwaI XcmI