Restriction map for
pFN21AB5876
(Circular) (Six-base) MAPSORT of: pFN21AB5876.seq Check: 7323 from: 1 to: 6190
pFN21AB5876 6190 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 3332 4317 4690 1563
Size: 1769 985 373 3063
Fragments arranged by size:
3063 1769 985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 3180 4253 5637 5905 1632
Size: 817 731 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1073 817 731 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2580 5089 6066 1203
Size: 1377 2509 977 1327
Fragments arranged by size:
2509 1377 1327 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 2290 4919 2290
Size: 2629 3561
AleI CACnn'nnGTG
Cuts at: 1166 2952 1166
Size: 1786 4404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3340 3372 3340
Size: 32 6158
AlwNI CAG_nnn'CTG
Cuts at: 2933 3933 5505 2933
Size: 1000 1572 3618
Fragments arranged by size:
3618 1572 1000
ApaI G_GGCC'C
Cuts at: 2082 2082
Size: 6190
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 2335 2422 2453 3244 3499 3725 3877 5986
Size: 842 87 31 791 255 226 152 2109
Cuts at: 5986 1493
Size: 1697
Fragments arranged by size:
2109 1697 842 791 255 226 152 87
31
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2228 2264 3515 1870
Size: 73 114 171 36 1251 4545
Fragments arranged by size:
4545 1251 171 114 73 36
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3511 4966 618
Size: 325 217 627 1724 1455 1842
Fragments arranged by size:
1842 1724 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2082 2236 3509 1391
Size: 691 154 1273 4072
Fragments arranged by size:
4072 1273 691 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2364 928
Size: 971 465 4754
Fragments arranged by size:
4754 971 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3407 3441 4381 4415 1903
Size: 34 1470 34 940 34 3678
Fragments arranged by size:
3678 1470 940 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3454 4082 5292 3454
Size: 628 1210 4352
Fragments arranged by size:
4352 1210 628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BfrBI ATG'CAT
Cuts at: 2665 2665
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 2010 3607 2010
Size: 1597 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2082 4251 5407 1790
Size: 292 2169 1156 2573
Fragments arranged by size:
2573 2169 1156 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 2284 2472
Size: 416 53 83 399 24 795 72 188
Cuts at: 2472 3921 4879 442
Size: 1449 958 1753
Fragments arranged by size:
1753 1449 958 795 416 399 188 83
72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 4850 1239
Size: 1446 2165 2579
Fragments arranged by size:
2579 2165 1446
Bpu10I CC'TnA_GC
Cuts at: 2084 2084
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4315 5180 5478 4315
Size: 865 298 5027
Fragments arranged by size:
5027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2286 3997 4379
Size: 53 83 186 936 717 36 1711 382
Cuts at: 4379 5014 275
Size: 635 1451
Fragments arranged by size:
1711 1451 936 717 635 382 186 83
53 36
BsaWI w'CCGG_w
Cuts at: 2977 3385 4623 5021 5295 5442 5853 2977
Size: 408 1238 398 274 147 411 3314
Fragments arranged by size:
3314 1238 411 408 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 5134
Fragments arranged by size:
5134 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2817 3053 1186
Size: 1631 236 4323
Fragments arranged by size:
4323 1631 236
BseYI C'CCAG_C
Cuts at: 2318 5393 5889 2318
Size: 3075 496 2619
Fragments arranged by size:
3075 2619 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2610 1163
Size: 573 874 4743
Fragments arranged by size:
4743 874 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3205 4251 4336 5407 3205
Size: 1046 85 1071 3988
Fragments arranged by size:
3988 1071 1046 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
BsmBI CGTCTCn'nnnn_
Cuts at: 2058 2058
Size: 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2236 3205 3509 4251 4336 5407
Size: 399 292 154 969 304 742 85 1071
Cuts at: 5407 1391
Size: 2174
Fragments arranged by size:
2174 1071 969 742 399 304 292 154
85
BspEI T'CCGG_A
Cuts at: 3385 5853 3385
Size: 2468 3722
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2133 4685 4859 65
Size: 1183 885 2552 174 1396
Fragments arranged by size:
2552 1396 1183 885 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4834 5021 1700
Size: 110 3024 187 2869
Fragments arranged by size:
3024 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 2630 4250 5262 2630
Size: 1620 1012 3558
Fragments arranged by size:
3558 1620 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 3339 4994 3339
Size: 1655 4535
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2992 3520 3887 4272 4289 5741 5827 5839
Size: 1020 528 367 385 17 1452 86 12
Cuts at: 5839 6185 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1020 528 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2102 2208 513
Size: 552 939 98 106 4495
Fragments arranged by size:
4495 939 552 106 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 3205 3425 3706 4500 4520 2091
Size: 1114 220 281 794 20 3761
Fragments arranged by size:
3761 1114 794 281 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 2554 3494 2554
Size: 940 5250
DraIII CAC_nnn'GTG
Cuts at: 2355 2355
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2323 2754 3637
Size: 54 1425 50 275 18 493 431 883
Cuts at: 3637 4526 6155 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 883 493 431 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 4120 1353
Size: 1439 1328 3423
Fragments arranged by size:
3423 1439 1328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2226 4777 5151 5297 387
Size: 1064 138 637 2551 374 146 1280
Fragments arranged by size:
2551 1280 1064 637 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 2685 3180 4253 4850 5637 5905
Size: 393 817 236 495 1073 597 787 268
Cuts at: 5905 1239
Size: 1524
Fragments arranged by size:
1524 1073 817 787 597 495 393 268
236
EcoO109I rG'GnC_Cy
Cuts at: 2078 2079 2078
Size: 1 6189
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3298 3298
Size: 6190
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2276 2308 2863
Size: 32 135 32 632 32 40 32 555
Cuts at: 2863 2895 3011 3043 3202 3234 4831 4863 4905
Size: 32 116 32 159 32 1597 32 42
Cuts at: 4905 4937 1373
Size: 32 2626
Fragments arranged by size:
2626 1597 632 555 159 135 116 42
40 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3280 748
Size: 2532 3658
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2771 5304 5488 1305
Size: 1466 2533 184 2007
Fragments arranged by size:
2533 2007 1466 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 3444
Fragments arranged by size:
3444 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2952 4148 4507 4666 518
Size: 648 1786 1196 359 159 2042
Fragments arranged by size:
2042 1786 1196 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2322 3606 4283 5431 5676 1606
Size: 498 218 1284 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 677 498 245 218
NcoI C'CATG_G
Cuts at: 513 1065 2004 2208 513
Size: 552 939 204 4495
Fragments arranged by size:
4495 939 552 204
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 2667 2667
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2051 2584 2669 3548 5093 1132
Size: 97 822 533 85 879 1545 2229
Fragments arranged by size:
2229 1545 879 822 533 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 2580 5089 2580
Size: 2509 3681
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2355 1108
Size: 1247 4943
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 2078 2078
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2416 3542 830
Size: 1586 1126 3478
Fragments arranged by size:
3478 1586 1126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
SacII CC_GC'GG
Cuts at: 2105 2105
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SexAI A'CCwGG_T
Cuts at: 3183 3183
Size: 6190
SfcI C'TryA_G
Cuts at: 826 1045 2412 3538 4673 5354 5545 826
Size: 219 1367 1126 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 1126 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 2230 2266 3517 1872
Size: 358 36 1251 4545
Fragments arranged by size:
4545 1251 358 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4294 5195 5457 820
Size: 197 926 2351 901 262 1553
Fragments arranged by size:
2351 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 2018 152
Size: 1866 4324
SphI G_CATG'C
Cuts at: 1132 2051 2669 3548 1132
Size: 919 618 879 3774
Fragments arranged by size:
3774 919 879 618
SspI AAT'ATT
Cuts at: 5 52 2761 4114 5
Size: 47 2709 1353 2081
Fragments arranged by size:
2709 2081 1353 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2004 2208 2330 3066 3629 513
Size: 552 939 204 122 736 563 3074
Fragments arranged by size:
3074 939 736 563 552 204 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4217 4234 4387 4572 5017 325
Size: 1255 2637 17 153 185 445 1498
Fragments arranged by size:
2637 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4436 6148 96
Size: 274 80 33 51 1443 2459 1712 138
Fragments arranged by size:
2459 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6150 6182 6150
Size: 32 6158
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 2915 3526 2915
Size: 611 5579
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 2228 2264 3515 1870
Size: 358 36 1251 4545
Fragments arranged by size:
4545 1251 358 36
XmnI GAAnn'nnTTC
Cuts at: 2402 3247 3375 4319 2402
Size: 845 128 944 4273
Fragments arranged by size:
4273 944 845 128
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI
SapI SfiI SfoI SrfI SwaI XcmI