Restriction map for
pFN21AB5875
(Circular) (Six-base) MAPSORT of: pFN21AB5875.seq Check: 1381 from: 1 to: 6100
pFN21AB5875 6100 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3443 1937
Size: 1506 4594
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 2673 4227 4600 1563
Size: 1110 1554 373 3063
Fragments arranged by size:
3063 1554 1110 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4163 5547 5815 1632
Size: 2531 1384 268 1917
Fragments arranged by size:
2531 1917 1384 268
AfeI AGC'GCT
Cuts at: 2358 2358
Size: 6100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 2041 2584 3843 5415 2041
Size: 543 1259 1572 2726
Fragments arranged by size:
2726 1572 1259 543
ApaI G_GGCC'C
Cuts at: 3039 3039
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3243 3409 3635 3787 5896 1493
Size: 1750 166 226 152 2109 1697
Fragments arranged by size:
2109 1750 1697 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 3317 3425 1870
Size: 73 221 1153 108 4545
Fragments arranged by size:
4545 1153 221 108 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 3421 4876 618
Size: 325 217 627 447 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 447 325 217
BanII G_rGCy'C
Cuts at: 1391 2802 3039 3419 1391
Size: 1411 237 380 4072
Fragments arranged by size:
4072 1411 380 237
BbeI G_GCGC'C
Cuts at: 2238 2238
Size: 6100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3390 928
Size: 971 1491 3638
Fragments arranged by size:
3638 1491 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2269 3039 4161 5317 1790
Size: 326 153 770 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 770 326 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 2761 3831
Size: 416 53 83 399 24 839 505 1070
Cuts at: 3831 4789 442
Size: 958 1753
Fragments arranged by size:
1753 1070 958 839 505 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2123 1056
Size: 1067 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2308 2432 2952 4760 1239
Size: 812 257 124 520 1808 2579
Fragments arranged by size:
2579 1808 812 520 257 124
Bpu10I CC'TnA_GC
Cuts at: 2585 3311 2585
Size: 726 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 3907 4289 4924
Size: 53 83 186 936 702 1672 382 635
Cuts at: 4924 275
Size: 1451
Fragments arranged by size:
1672 1451 936 702 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2306 2336 1178
Size: 30 165 30 903 30 4942
Fragments arranged by size:
4942 903 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2209 2246 2288 3189 3338 1186
Size: 1023 37 42 901 149 3948
Fragments arranged by size:
3948 1023 901 149 42 37
BseYI C'CCAG_C
Cuts at: 2359 3211 5303 5799 2359
Size: 852 2092 496 2660
Fragments arranged by size:
2660 2092 852 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2466 2827 1163
Size: 573 730 361 4436
Fragments arranged by size:
4436 730 573 361
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 4161 4246 5317 2802
Size: 1359 85 1071 3585
Fragments arranged by size:
3585 1359 1071 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
BsmBI CGTCTCn'nnnn_
Cuts at: 2553 2553
Size: 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2269 2802 3039 3419 4161 4246
Size: 399 326 153 533 237 380 742 85
Cuts at: 4246 5317 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 533 399 380 326 237
153 85
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3455 844
Size: 2611 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 3987 1394
Size: 429 204 1960 3507
Fragments arranged by size:
3507 1960 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2246 2288 4744 4931 1700
Size: 110 436 42 2456 187 2869
Fragments arranged by size:
2869 2456 436 187 110 42
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 2917 4904 2917
Size: 1987 4113
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5240
BstXI CCAn_nnnn'nTGG
Cuts at: 2677 3142 2677
Size: 465 5635
BstYI r'GATC_y
Cuts at: 1972 2181 3430 3797 4182 4199 5651 5737 5749
Size: 209 1249 367 385 17 1452 86 12
Cuts at: 5749 6095 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1249 385 367 346 209 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4513
Fragments arranged by size:
4513 1035 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 2114 3616 4410 4430 2114
Size: 1502 794 20 3784
Fragments arranged by size:
3784 1502 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 2454 3404 2454
Size: 950 5150
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065
Size: 54 1425 50 275 18 1717 889 1629
Cuts at: 6065 8
Size: 43
Fragments arranged by size:
1717 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2470 2805 3057 3350 4030 1353
Size: 1117 335 252 293 680 3423
Fragments arranged by size:
3423 1117 680 335 293 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2308 2432 2952 4163 4760 5547
Size: 393 419 257 124 520 1211 597 787
Cuts at: 5547 5815 1239
Size: 268 1524
Fragments arranged by size:
1524 1211 787 597 520 419 393 268
257 124
EcoNI CCTnn'n_nnAGG
Cuts at: 3320 3320
Size: 6100
EcoO109I rG'GnC_Cy
Cuts at: 3035 3035
Size: 6100
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
EcoRV GAT'ATC
Cuts at: 2665 2665
Size: 6100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2910 2910
Size: 6100
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2238 2360 5247 1678
Size: 560 122 2887 2531
Fragments arranged by size:
2887 2531 560 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373
Size: 32 135 32 3169 32 42 32 2626
Fragments arranged by size:
3169 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3444 3690 669
Size: 988 281 927 579 246 3079
Fragments arranged by size:
3079 988 927 579 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 2865 3690 2865
Size: 825 5275
KasI G'GCGC_C
Cuts at: 2234 2234
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3276 5214 5398 1305
Size: 1971 1938 184 2007
Fragments arranged by size:
2007 1971 1938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4058 4417 4576 518
Size: 648 2892 359 159 2042
Fragments arranged by size:
2892 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2090 2153 2376 3516 4193 5341 5586 1606
Size: 484 63 223 1140 677 1148 245 2120
Fragments arranged by size:
2120 1148 1140 677 484 245 223 63
NaeI GCC'GGC
Cuts at: 2248 2290 2248
Size: 42 6058
NarI GG'CG_CC
Cuts at: 2235 2235
Size: 6100
NcoI C'CATG_G
Cuts at: 513 1065 2100 513
Size: 552 1035 4513
Fragments arranged by size:
4513 1035 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NgoMIV G'CCGG_C
Cuts at: 2246 2288 2246
Size: 42 6058
NheI G'CTAG_C
Cuts at: 1052 2119 1052
Size: 1067 5033
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2761 3458 5003 1132
Size: 97 1532 697 1545 2229
Fragments arranged by size:
2229 1545 1532 697 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2728 1108
Size: 1620 4480
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 3035 3035
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2095 2346 2738 3452 830
Size: 1265 251 392 714 3478
Fragments arranged by size:
3478 1265 714 392 251
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2470 2470
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SexAI A'CCwGG_T
Cuts at: 2040 2040
Size: 6100
SfcI C'TryA_G
Cuts at: 826 1045 2091 2342 2734 3448 4583 5264 5455
Size: 219 1046 251 392 714 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1135 1046 714 681 392 251 219
191
SfoI GGC'GCC
Cuts at: 2236 2236
Size: 6100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 2166 3427 1872
Size: 294 1261 4545
Fragments arranged by size:
4545 1261 294
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4204 5105 5367 820
Size: 197 926 2261 901 262 1553
Fragments arranged by size:
2261 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 2761 3458 1132
Size: 1629 697 3774
Fragments arranged by size:
3774 1629 697
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 2778 1517
Size: 1261 4839
StyI C'CwwG_G
Cuts at: 513 1065 2100 2463 3031 3539 513
Size: 552 1035 363 568 508 3074
Fragments arranged by size:
3074 1035 568 552 508 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 4127 4144 4297 4482 4927 325
Size: 1255 1230 1317 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 1230 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 6058 96
Size: 274 80 33 51 1443 2369 1712 138
Fragments arranged by size:
2369 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 3255 3287 6060 6092 2306
Size: 32 917 32 2773 32 2314
Fragments arranged by size:
2773 2314 917 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2597 3107 2597
Size: 510 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 2164 3425 1870
Size: 294 1261 4545
Fragments arranged by size:
4545 1261 294
XmnI GAAnn'nnTTC
Cuts at: 3193 4229 3193
Size: 1036 5064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SfiI SrfI SwaI