Restriction map for
pFN21AB5867
(Circular) (Six-base) MAPSORT of: pFN21AB5867.seq Check: 5833 from: 1 to: 6091
pFN21AB5867 6091 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2809 3434 1937
Size: 872 625 4594
Fragments arranged by size:
4594 872 625
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 3288 4154 5538 5806 1632
Size: 870 786 866 1384 268 1917
Fragments arranged by size:
1917 1384 870 866 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3110 1184
Size: 1926 4165
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2519 3230 3400 3626 3778 5887 1493
Size: 1026 711 170 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 711 226 170 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3263 3412 4867 618
Size: 325 217 627 1476 149 1455 1842
Fragments arranged by size:
1842 1476 1455 627 325 217 149
BanII G_rGCy'C
Cuts at: 1391 3410 1391
Size: 2019 4072
BbeI G_GCGC'C
Cuts at: 3267 3267
Size: 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 2562 2844 2949 928
Size: 971 203 460 282 105 4070
Fragments arranged by size:
4070 971 460 282 203 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3101 3135 4282 4316 1903
Size: 34 1164 34 1147 34 3678
Fragments arranged by size:
3678 1164 1147 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BfrBI ATG'CAT
Cuts at: 2633 2633
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2665 6086 2665
Size: 3421 2670
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2434 4152 5308 1790
Size: 644 1718 1156 2573
Fragments arranged by size:
2573 1718 1156 644
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 4751 1239
Size: 886 2626 2579
Fragments arranged by size:
2626 2579 886
Bpu10I CC'TnA_GC
Cuts at: 2028 2184 2028
Size: 156 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2754 4754 882
Size: 617 1255 2000 2219
Fragments arranged by size:
2219 2000 1255 617
BsaAI yAC'GTr
Cuts at: 493 1204 3355 3882 493
Size: 711 2151 527 2702
Fragments arranged by size:
2702 2151 711 527
BsaBI GATnn'nnATC
Cuts at: 2709 6085 2709
Size: 3376 2715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3264 3898 4280 4915
Size: 53 83 186 936 1731 634 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1731 1451 936 635 634 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2706 1186
Size: 1520 4571
BseYI C'CCAG_C
Cuts at: 2647 5294 5790 2647
Size: 2647 496 2948
Fragments arranged by size:
2948 2647 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2930 4152 4237 5308 2930
Size: 1222 85 1071 3713
Fragments arranged by size:
3713 1222 1071 85
BsmI GAATG_Cn'
Cuts at: 3138 3602 3695 3138
Size: 464 93 5534
Fragments arranged by size:
5534 464 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2434 2930 3410 4152 4237 5308 1391
Size: 399 644 496 480 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 496 480 399 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3292 3446 844
Size: 2448 154 3489
Fragments arranged by size:
3489 2448 154
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2021 794
Size: 1227 4864
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2665 2704 3116 3340 3421 3788 4173 4190
Size: 693 39 412 224 81 367 385 17
Cuts at: 4190 5642 5728 5740 6086 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 693 412 385 367 346 224
86 81 39 17 12
BstZ17I GTA'TAC
Cuts at: 2810 2810
Size: 6091
Bsu36I CC'TnA_GG
Cuts at: 2847 2847
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 2079 2157 2608 513
Size: 552 1014 78 451 3996
Fragments arranged by size:
3996 1014 552 451 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2122 2239 3092 3607 4401 4421 2122
Size: 117 853 515 794 20 3792
Fragments arranged by size:
3792 853 794 515 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 2125 2139 3395 2125
Size: 14 1256 4821
Fragments arranged by size:
4821 1256 14
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2236 3349
Size: 54 1425 50 275 18 241 165 1113
Cuts at: 3349 3538 4427 6056 8
Size: 189 889 1629 43
Fragments arranged by size:
1629 1425 1113 889 275 241 189 165
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2292 2319 2466 4021 1353
Size: 829 110 27 147 1555 3423
Fragments arranged by size:
3423 1555 829 147 110 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2502 3288 4154 4751 5538 5806
Size: 393 493 377 786 866 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 866 787 786 597 493 393 377
268
EcoRI G'AATT_C
Cuts at: 2519 3400 3778 2519
Size: 881 378 4832
Fragments arranged by size:
4832 881 378
EcoRV GAT'ATC
Cuts at: 2796 2796
Size: 6091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2991 2293
Size: 698 5393
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2194 3267 5238 1678
Size: 516 1073 1971 2531
Fragments arranged by size:
2531 1971 1073 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373
Size: 32 135 32 3160 32 42 32 2626
Fragments arranged by size:
3160 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2602 748
Size: 1854 4237
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 3263 3263
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2823 5205 5389 1305
Size: 1518 2382 184 2007
Fragments arranged by size:
2382 2007 1518 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2238 3351 10
Size: 54 1425 343 406 1113 2750
Fragments arranged by size:
2750 1425 1113 406 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4049 4408 4567 518
Size: 648 2883 359 159 2042
Fragments arranged by size:
2883 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2651 2765 3507 4184 5332 5577 1606
Size: 1045 114 742 677 1148 245 2120
Fragments arranged by size:
2120 1148 1045 742 677 245 114
NarI GG'CG_CC
Cuts at: 3264 3264
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2079 2157 513
Size: 552 1014 78 4447
Fragments arranged by size:
4447 1014 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NsiI A_TGCA'T
Cuts at: 2635 2635
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2213 3449 4994 1132
Size: 97 984 1236 1545 2229
Fragments arranged by size:
2229 1545 1236 984 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PmlI CAC'GTG
Cuts at: 3355 3355
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 2662 3443 830
Size: 1832 781 3478
Fragments arranged by size:
3478 1832 781
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2651 2765 2651
Size: 114 5977
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2164 2658 2932 3439 4574 5255 5446
Size: 219 1119 494 274 507 1135 681 191
Cuts at: 5446 826
Size: 1471
Fragments arranged by size:
1471 1135 1119 681 507 494 274 219
191
SfoI GGC'GCC
Cuts at: 3265 3265
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2213 3449 1132
Size: 1081 1236 3774
Fragments arranged by size:
3774 1236 1081
SspI AAT'ATT
Cuts at: 5 52 2143 4015 5
Size: 47 2091 1872 2081
Fragments arranged by size:
2091 2081 1872 47
StuI AGG'CCT
Cuts at: 1517 2458 2950 1517
Size: 941 492 4658
Fragments arranged by size:
4658 941 492
StyI C'CwwG_G
Cuts at: 513 1065 2079 2157 3530 513
Size: 552 1014 78 1373 3074
Fragments arranged by size:
3074 1373 1014 552 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2413 2601 3335 4118 4135 4288 4473
Size: 1255 833 188 734 783 17 153 185
Cuts at: 4473 4918 325
Size: 445 1498
Fragments arranged by size:
1498 1255 833 783 734 445 188 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2989 3021 6051 6083 2989
Size: 32 3030 32 2997
Fragments arranged by size:
3030 2997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I
FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI