Restriction map for
pFN21AB5864
(Circular) (Six-base) MAPSORT of: pFN21AB5864.seq Check: 6478 from: 1 to: 6178
pFN21AB5864 6178 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2121 2121
Size: 6178
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2754 3233 4241 5625 5893 1632
Size: 1122 479 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1122 1008 479 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 3414 1166
Size: 2248 3930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AlwNI CAG_nnn'CTG
Cuts at: 2041 2050 3921 5493 2041
Size: 9 1871 1572 2726
Fragments arranged by size:
2726 1871 1572 9
ApaI G_GGCC'C
Cuts at: 2077 2077
Size: 6178
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 3136 3487 3713 3865 5974 1493
Size: 1643 351 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 351 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 3290 3503 1870
Size: 73 66 1281 213 4545
Fragments arranged by size:
4545 1281 213 73 66
BamHI G'GATC_C
Cuts at: 2433 3508 3875 2433
Size: 1075 367 4736
Fragments arranged by size:
4736 1075 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3499 4954 618
Size: 325 217 627 1712 1455 1842
Fragments arranged by size:
1842 1712 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2077 2298 3497 1391
Size: 670 16 221 1199 4072
Fragments arranged by size:
4072 1199 670 221 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2524 928
Size: 971 625 4582
Fragments arranged by size:
4582 971 625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2045 2079 4369 4403 1903
Size: 34 50 34 24 34 2290 34 3678
Fragments arranged by size:
3678 2290 50 34 34 34 34 24
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 4789 136
Size: 107 122 71 631 605 400 2717 1525
Fragments arranged by size:
2717 1525 631 605 400 122 107 71
BglII A'GATC_T
Cuts at: 2787 6173 2787
Size: 3386 2792
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2077 2452 4239 5395 1790
Size: 278 9 375 1787 1156 2573
Fragments arranged by size:
2573 1787 1156 375 278 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3909 4867 442
Size: 416 53 83 399 24 2492 958 1753
Fragments arranged by size:
2492 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 3433 4838 1239
Size: 860 1334 1405 2579
Fragments arranged by size:
2579 1405 1334 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2198 2312 3399 4841 882
Size: 617 699 114 1087 1442 2219
Fragments arranged by size:
2219 1442 1087 699 617 114
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 3985 4367 5002
Size: 53 83 186 936 924 1528 382 635
Cuts at: 5002 275
Size: 1451
Fragments arranged by size:
1528 1451 936 924 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2878 4611 5009 5283 5430 5841 2878
Size: 1733 398 274 147 411 3215
Fragments arranged by size:
3215 1733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2121 2151 2635 2665 1178
Size: 30 165 30 718 30 484 30 4691
Fragments arranged by size:
4691 718 484 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2368 1186
Size: 1182 4996
BseYI C'CCAG_C
Cuts at: 2238 3397 5381 5877 2238
Size: 1159 1984 496 2539
Fragments arranged by size:
2539 1984 1159 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 1163
Size: 573 750 4855
Fragments arranged by size:
4855 750 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2085 4239 4324 5395 2061
Size: 24 2154 85 1071 2844
Fragments arranged by size:
2844 2154 1071 85 24
BsmI GAATG_Cn'
Cuts at: 3457 3689 3782 3457
Size: 232 93 5853
Fragments arranged by size:
5853 232 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2638 2638
Size: 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2068 2077 2085 2298 2452 3497
Size: 399 271 7 9 8 213 154 1045
Cuts at: 3497 4239 4324 5395 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1045 742 399 271 213 154
85 9 8 7
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 2950 4067 5797 2950
Size: 1117 1730 3331
Fragments arranged by size:
3331 1730 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 3533 844
Size: 1277 1412 3489
Fragments arranged by size:
3489 1412 1277
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3153 3444 4673 4847 65
Size: 1183 1905 291 1229 174 1396
Fragments arranged by size:
1905 1396 1229 1183 291 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6178
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2433 2773 2787 3058 3169 3508 3875 4260
Size: 461 340 14 271 111 339 367 385
Cuts at: 4260 4277 5729 5815 5827 6173 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 461 385 367 346 340 339
271 111 86 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2068 2246 2452 513
Size: 552 1003 178 206 4239
Fragments arranged by size:
4239 1003 552 206 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 2181 3694 4488 4508 2181
Size: 1513 794 20 3851
Fragments arranged by size:
3851 1513 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2647 2682 529
Size: 35 2083 35 4025
Fragments arranged by size:
4025 2083 35 35
DraI TTT'AAA
Cuts at: 2688 3141 3482 2688
Size: 453 341 5384
Fragments arranged by size:
5384 453 341
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2962 3625
Size: 54 1425 50 275 18 222 910 663
Cuts at: 3625 4514 6143 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 663 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 3212 4108 1353
Size: 869 990 896 3423
Fragments arranged by size:
3423 990 896 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2059 2059
Size: 6178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2754 3233 3433 4241 4838 5625
Size: 393 467 655 479 200 808 597 787
Cuts at: 5625 5893 1239
Size: 268 1524
Fragments arranged by size:
1524 808 787 655 597 479 467 393
268 200
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3023
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 2073 2074 2073
Size: 1 6177
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
EcoRV GAT'ATC
Cuts at: 2152 3031 2152
Size: 879 5299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2042 2473 5325 1678
Size: 364 431 2852 2531
Fragments arranged by size:
2852 2531 431 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2153 2758 2790 2927
Size: 32 135 32 549 32 605 32 137
Cuts at: 2927 2959 4819 4851 4893 4925 1373
Size: 32 1860 32 42 32 2626
Fragments arranged by size:
2626 1860 605 549 137 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2580 3522 3768 669
Size: 988 281 642 942 246 3079
Fragments arranged by size:
3079 988 942 642 281 246
HindIII A'AGCT_T
Cuts at: 748 2909 748
Size: 2161 4017
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3119 5292 5476 1305
Size: 1814 2173 184 2007
Fragments arranged by size:
2173 2007 1814 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4356
Fragments arranged by size:
4356 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3414 4136 4495 4654 518
Size: 648 2199 49 722 359 159 2042
Fragments arranged by size:
2199 2042 722 648 359 159 49
MspA1I CmG'CkG
Cuts at: 1606 2047 2091 3594 4271 5419 5664 1606
Size: 441 44 1503 677 1148 245 2120
Fragments arranged by size:
2120 1503 1148 677 441 245 44
NcoI C'CATG_G
Cuts at: 513 1065 2452 513
Size: 552 1387 4239
Fragments arranged by size:
4239 1387 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 3536 5081 1132
Size: 97 2307 1545 2229
Fragments arranged by size:
2307 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3006 1348
Size: 1658 4520
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2971 3101 1108
Size: 1863 130 4185
Fragments arranged by size:
4185 1863 130
PfoI T'CCnGG_A
Cuts at: 2436 2436
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3125 3748 3125
Size: 623 5555
PspOMI G'GGCC_C
Cuts at: 2073 2073
Size: 6178
PstI C_TGCA'G
Cuts at: 830 2046 3530 830
Size: 1216 1484 3478
Fragments arranged by size:
3478 1484 1216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 2047 2091 2047
Size: 44 6134
SacI G_AGCT'C
Cuts at: 2061 2061
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SfcI C'TryA_G
Cuts at: 826 1045 2042 3526 4661 5342 5533 826
Size: 219 997 1484 1135 681 191 1471
Fragments arranged by size:
1484 1471 1135 997 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2011 3505 1872
Size: 139 1494 4545
Fragments arranged by size:
4545 1494 139
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4282 5183 5445 820
Size: 197 926 2339 901 262 1553
Fragments arranged by size:
2339 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 2262 1517
Size: 745 5433
StyI C'CwwG_G
Cuts at: 513 1065 2452 2751 3617 513
Size: 552 1387 299 866 3074
Fragments arranged by size:
3074 1387 866 552 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 4205 4222 4375 4560 5005 325
Size: 1255 858 1767 17 153 185 445 1498
Fragments arranged by size:
1767 1498 1255 858 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4424 6136 96
Size: 274 80 33 51 1443 2447 1712 138
Fragments arranged by size:
2447 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 3443 3475 6138 6170 3235
Size: 32 176 32 2663 32 3243
Fragments arranged by size:
3243 2663 176 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 3325 3052
Size: 273 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 2009 3503 1870
Size: 139 1494 4545
Fragments arranged by size:
4545 1494 139
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI