Restriction map for
pFN21AB5863
(Circular) (Six-base) MAPSORT of: pFN21AB5863.seq Check: 2523 from: 1 to: 6088
pFN21AB5863 6088 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2671 2671
Size: 6088
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 3431 1937
Size: 328 1166 4594
Fragments arranged by size:
4594 1166 328
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2341 4151 5535 5803 1632
Size: 709 1810 1384 268 1917
Fragments arranged by size:
1917 1810 1384 709 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2113 2379 2676 3831 5403 2113
Size: 266 297 1155 1572 2798
Fragments arranged by size:
2798 1572 1155 297 266
ApaI G_GGCC'C
Cuts at: 3219 3219
Size: 6088
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2049 2217 3397 3623 3775 5884 1493
Size: 556 168 1180 226 152 2109 1697
Fragments arranged by size:
2109 1697 1180 556 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 3413 1870
Size: 73 1233 237 4545
Fragments arranged by size:
4545 1233 237 73
BamHI G'GATC_C
Cuts at: 2118 3336 3418 3785 2118
Size: 1218 82 367 4421
Fragments arranged by size:
4421 1218 367 82
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3239 3382 3409 4864 618
Size: 325 217 627 1452 143 27 1455 1842
Fragments arranged by size:
1842 1455 1452 627 325 217 143 27
BanII G_rGCy'C
Cuts at: 1391 2154 2994 3219 3288 3407 1391
Size: 763 840 225 69 119 4072
Fragments arranged by size:
4072 840 763 225 119 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2616 2625 2851 928
Size: 971 717 9 226 4165
Fragments arranged by size:
4165 971 717 226 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2320 2354 4279 4313 1903
Size: 34 383 34 1925 34 3678
Fragments arranged by size:
3678 1925 383 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2376 2720 4699
Size: 107 122 71 631 605 704 344 1979
Cuts at: 4699 136
Size: 1525
Fragments arranged by size:
1979 1525 704 631 605 344 122 107
71
BglII A'GATC_T
Cuts at: 2190 6083 2190
Size: 3893 2195
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2328 2955 3219 3348 3385 4149 5305
Size: 314 224 627 264 129 37 764 1156
Cuts at: 5305 1790
Size: 2573
Fragments arranged by size:
2573 1156 764 627 314 264 224 129
37
BmgBI CAC'GTC
Cuts at: 1318 2263 1318
Size: 945 5143
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2165 2189 4748 1239
Size: 832 94 24 2559 2579
Fragments arranged by size:
2579 2559 832 94 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3005 4751 882
Size: 617 1506 1746 2219
Fragments arranged by size:
2219 1746 1506 617
BsaAI yAC'GTr
Cuts at: 493 1204 2334 3879 493
Size: 711 1130 1545 2702
Fragments arranged by size:
2702 1545 1130 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2050 2080 2162 2192 2288
Size: 30 165 30 647 30 82 30 96
Cuts at: 2288 2318 1178
Size: 30 4948
Fragments arranged by size:
4948 647 165 96 82 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 2162 2201 2219 2477 1186
Size: 872 104 39 18 258 4797
Fragments arranged by size:
4797 872 258 104 39 18
BseYI C'CCAG_C
Cuts at: 2379 2506 2820 2922 3027 3267 3378 5291 5787
Size: 127 314 102 105 240 111 1913 496
Cuts at: 5787 2379
Size: 2680
Fragments arranged by size:
2680 1913 496 314 240 127 111 105
102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2428 2897 3142 1163
Size: 573 692 469 245 4109
Fragments arranged by size:
4109 692 573 469 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2301 2730 2888 2994 4149 4234 5305 2154
Size: 147 429 158 106 1155 85 1071 2937
Fragments arranged by size:
2937 1155 1071 429 158 147 106 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2154 2301 2328 2730 2888 2955
Size: 399 314 50 147 27 402 158 67
Cuts at: 2955 2994 3219 3288 3348 3385 3407 4149 4234
Size: 39 225 69 60 37 22 742 85
Cuts at: 4234 5305 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 314 225 158
147 85 69 67 60 50 39 37
27 22
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3132 3977 5707 3132
Size: 845 1730 3513
Fragments arranged by size:
3513 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2671 2820 3443 844
Size: 1827 149 623 3489
Fragments arranged by size:
3489 1827 623 149
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 3975 1394
Size: 429 350 1802 3507
Fragments arranged by size:
3507 1802 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3002 3183 4732 4919 1700
Size: 110 1192 181 1549 187 2869
Fragments arranged by size:
2869 1549 1192 187 181 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 3017 4148 5160 3017
Size: 1131 1012 3945
Fragments arranged by size:
3945 1131 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 2270 1184
Size: 1086 5002
BstYI r'GATC_y
Cuts at: 1972 2118 2190 2629 3336 3418 3785 4170 4187
Size: 146 72 439 707 82 367 385 17
Cuts at: 4187 5639 5725 5737 6083 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 707 439 385 367 346 146
86 82 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2633 3102 3219 3235 3340 513
Size: 552 1568 469 117 16 105 3261
Fragments arranged by size:
3261 1568 552 469 117 105 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2887 487
Size: 683 56 1661 3688
Fragments arranged by size:
3688 1661 683 56
BtsI GCAGTG_nn'
Cuts at: 2531 2544 2730 2761 2906 3604 4398 4418 2531
Size: 13 186 31 145 698 794 20 4201
Fragments arranged by size:
4201 794 698 186 145 31 20 13
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 2110 2110
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 3004 3535
Size: 54 1425 50 275 18 207 967 531
Cuts at: 3535 4424 6053 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 967 889 531 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3368 4018 1353
Size: 2015 650 3423
Fragments arranged by size:
3423 2015 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2992 2152
Size: 840 5248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2165 2189 2341 4151 4748 5535
Size: 393 439 94 24 152 1810 597 787
Cuts at: 5535 5803 1239
Size: 268 1524
Fragments arranged by size:
1810 1524 787 597 439 393 268 152
94 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2559 3215 2559
Size: 656 5432
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6088
FseI GG_CCGG'CC
Cuts at: 3006 3006
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2482 2821 5235 1678
Size: 804 339 2414 2531
Fragments arranged by size:
2531 2414 804 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4729 4761 4803 4835 1373
Size: 32 135 32 3157 32 42 32 2626
Fragments arranged by size:
3157 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 3432 3678 669
Size: 988 281 328 1166 246 3079
Fragments arranged by size:
3079 1166 988 328 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 2419 3687 2419
Size: 1268 4820
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 10
Size: 54 1425 343 207 4059
Fragments arranged by size:
4059 1425 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2361 2376 2635 2756 2973 3504 4181 5329
Size: 755 15 259 121 217 531 677 1148
Cuts at: 5329 5574 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 755 677 531 259 245 217
121 15
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 3219 3340 513
Size: 552 2154 121 3261
Fragments arranged by size:
3261 2154 552 121
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3160 3446 4991 1132
Size: 97 1931 286 1545 2229
Fragments arranged by size:
2229 1931 1545 286 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 2281 2767 1108
Size: 1122 51 486 4429
Fragments arranged by size:
4429 1122 486 51
PfoI T'CCnGG_A
Cuts at: 2142 2964 2142
Size: 822 5266
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2334 2334
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 3215 3215
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2526 3440 830
Size: 1696 914 3478
Fragments arranged by size:
3478 1696 914
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2361 2361
Size: 6088
SacI G_AGCT'C
Cuts at: 2154 2994 2154
Size: 840 5248
SacII CC_GC'GG
Cuts at: 2636 2636
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 2264 3430 1936
Size: 328 1166 4594
Fragments arranged by size:
4594 1166 328
SapI GCTCTTCn'nnn_
Cuts at: 3368 3368
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 2106 3242 2106
Size: 1136 4952
SfcI C'TryA_G
Cuts at: 826 1045 2482 2522 2875 3436 4571 5252 5443
Size: 219 1437 40 353 561 1135 681 191
Cuts at: 5443 826
Size: 1471
Fragments arranged by size:
1471 1437 1135 681 561 353 219 191
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2211 2292 3219 3340 3527 513
Size: 552 1146 81 927 121 187 3074
Fragments arranged by size:
3074 1146 927 552 187 121 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2734 4115 4132 4285 4470 4915 325
Size: 1255 1154 1381 17 153 185 445 1498
Fragments arranged by size:
1498 1381 1255 1154 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 2162 2194 2288 2320 6048 6080 2050
Size: 32 80 32 94 32 3728 32 2058
Fragments arranged by size:
3728 2058 94 80 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2140 2140
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FspAI KasI NarI NruI NsiI PacI PpuMI PspXI
PsrI RsrII SanDI SfiI SfoI SrfI SwaI