Restriction map for
pFN21AB5860
(Circular) (Six-base) MAPSORT of: pFN21AB5860.seq Check: 1443 from: 1 to: 6175
pFN21AB5860 6175 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2114 2114
Size: 6175
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2266 3985 278
Size: 53 83 186 936 730 1719 2468
Fragments arranged by size:
2468 1719 936 730 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2432 4238 5622 5890 1632
Size: 800 1806 1384 268 1917
Fragments arranged by size:
1917 1806 1384 800 268
AflII C'TTAA_G
Cuts at: 820 1017 2872 820
Size: 197 1855 4123
Fragments arranged by size:
4123 1855 197
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 2866 4904 2866
Size: 2038 4137
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 2351 3918 5490 2351
Size: 1567 1572 3036
Fragments arranged by size:
3036 1572 1567
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2533 2936 3182 3484 3710 3862 5971 1493
Size: 1040 403 246 302 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 403 302 246 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 1870
Size: 73 1557 4545
Fragments arranged by size:
4545 1557 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2874 2907 2874
Size: 33 6142
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3494 1391
Size: 2103 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 2526 3406 928
Size: 971 567 60 880 3697
Fragments arranged by size:
3697 971 880 567 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 2203 1548
Size: 655 5520
BfrBI ATG'CAT
Cuts at: 2499 2499
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2239 6170 2239
Size: 3931 2244
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 3033 1318
Size: 1715 4460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 3906 4864
Size: 416 53 83 399 24 850 1639 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
1753 1639 958 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 3241 2917
Size: 324 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2876 4835 1239
Size: 1637 1959 2579
Fragments arranged by size:
2579 1959 1637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 3001 3211 4300 5165 5463 2641
Size: 360 210 1089 865 298 3353
Fragments arranged by size:
3353 1089 865 360 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3212 4838 882
Size: 617 1713 1626 2219
Fragments arranged by size:
2219 1713 1626 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 3982 4364 4999
Size: 53 83 186 936 730 1719 382 635
Cuts at: 4999 275
Size: 1451
Fragments arranged by size:
1719 1451 936 730 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5950
Fragments arranged by size:
5950 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2591 1186
Size: 1405 4770
BseYI C'CCAG_C
Cuts at: 3413 5378 5874 3413
Size: 1965 496 3714
Fragments arranged by size:
3714 1965 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 1163
Size: 573 706 4896
Fragments arranged by size:
4896 706 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4236 4321 5392 4236
Size: 85 1071 5019
Fragments arranged by size:
5019 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3686 3779 2933
Size: 753 93 5329
Fragments arranged by size:
5329 753 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3494 4236 4321 5392 1391
Size: 399 1704 742 85 1071 2174
Fragments arranged by size:
2174 1704 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2114 3530 844
Size: 1270 1416 3489
Fragments arranged by size:
3489 1416 1270
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4670 4844 65
Size: 1183 3422 174 1396
Fragments arranged by size:
3422 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 3336 4235 5247 3336
Size: 899 1012 4264
Fragments arranged by size:
4264 1012 899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2126 794
Size: 1332 4843
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 2896 1184
Size: 1712 4463
BstYI r'GATC_y
Cuts at: 1972 2058 2239 3505 3872 4257 4274 5726 5812
Size: 86 181 1266 367 385 17 1452 86
Cuts at: 5812 5824 6170 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1266 385 367 346 181 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5623
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 2334 2474 2924 3042 3691 4485 4505 2334
Size: 140 450 118 649 794 20 4004
Fragments arranged by size:
4004 794 649 450 140 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140
Size: 54 1425 50 275 18 1792 889 1629
Cuts at: 6140 8
Size: 43
Fragments arranged by size:
1792 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4105 1353
Size: 2752 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4762 5136 5282 387
Size: 1064 138 3173 374 146 1280
Fragments arranged by size:
3173 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2432 2876 4238 4835 5622 5890 1239
Size: 393 800 444 1362 597 787 268 1524
Fragments arranged by size:
1524 1362 800 787 597 444 393 268
EcoRI G'AATT_C
Cuts at: 3182 3484 3862 3182
Size: 302 378 5495
Fragments arranged by size:
5495 378 302
EcoRV GAT'ATC
Cuts at: 2467 3040 2467
Size: 573 5602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2454 2454
Size: 6175
FspI TGC'GCA
Cuts at: 2130 4681 2130
Size: 2551 3624
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2350 2382 2537 2569 2723
Size: 32 135 32 778 32 155 32 154
Cuts at: 2723 2755 2852 2884 3355 3387 4816 4848 4890
Size: 32 97 32 471 32 1429 32 42
Cuts at: 4890 4922 1373
Size: 32 2626
Fragments arranged by size:
2626 1429 778 471 155 154 135 97
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3519 3765 669
Size: 988 281 1077 504 246 3079
Fragments arranged by size:
3079 1077 988 504 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3015 3765 3015
Size: 750 5425
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 5289 5473 1305
Size: 1432 2552 184 2007
Fragments arranged by size:
2552 2007 1432 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 4133 4492 4651 518
Size: 648 1020 1947 359 159 2042
Fragments arranged by size:
2042 1947 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3451 3591 4268 5416 5661 1606
Size: 1845 140 677 1148 245 2120
Fragments arranged by size:
2120 1845 1148 677 245 140
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5623
NdeI CA'TA_TG
Cuts at: 387 2220 387
Size: 1833 4342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NsiI A_TGCA'T
Cuts at: 2501 2501
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 2120 3533 5078 1132
Size: 97 891 1413 1545 2229
Fragments arranged by size:
2229 1545 1413 891 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PfoI T'CCnGG_A
Cuts at: 2065 2065
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 3527 830
Size: 2697 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 3451 3451
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2343 2454 2706 3523 4658 5339 5530
Size: 219 1298 111 252 817 1135 681 191
Cuts at: 5530 826
Size: 1471
Fragments arranged by size:
1471 1298 1135 817 681 252 219 191
111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2620 2872 2980 3226 4279 5180
Size: 197 926 677 252 108 246 1053 901
Cuts at: 5180 5442 820
Size: 262 1553
Fragments arranged by size:
1553 1053 926 901 677 262 252 246
197 108
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 2120 3533 1132
Size: 988 1413 3774
Fragments arranged by size:
3774 1413 988
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2566 2600 3614 513
Size: 552 1501 34 1014 3074
Fragments arranged by size:
3074 1501 1014 552 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4202 4219 4372 4557 5002 325
Size: 1255 2622 17 153 185 445 1498
Fragments arranged by size:
2622 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 2921 4421
Size: 274 80 33 51 1443 201 743 1500
Cuts at: 4421 6133 96
Size: 1712 138
Fragments arranged by size:
1712 1500 1443 743 274 201 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2800 2832 6135 6167 2800
Size: 32 3303 32 2808
Fragments arranged by size:
3303 2808 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 2507 3511 2507
Size: 1004 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3229 3229
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 3100 4304 3100
Size: 1204 4971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2264 3983 276
Size: 53 83 186 936 730 1719 2468
Fragments arranged by size:
2468 1719 936 730 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI