Restriction map for
pFN21AB5858
(Circular) (Six-base) MAPSORT of: pFN21AB5858.seq Check: 1216 from: 1 to: 6175
pFN21AB5858 6175 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2243 2174
Size: 69 6106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 2775 4302 4675 1563
Size: 1212 1527 373 3063
Fragments arranged by size:
3063 1527 1212 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2822 2873 4238 5622 5890 1632
Size: 455 735 51 1365 1384 268 1917
Fragments arranged by size:
1917 1384 1365 735 455 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 2828 5074 6051 1203
Size: 1625 2246 977 1327
Fragments arranged by size:
2246 1625 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 6143
AlwNI CAG_nnn'CTG
Cuts at: 3918 5490 3918
Size: 1572 4603
ApaI G_GGCC'C
Cuts at: 2719 2719
Size: 6175
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 3368 3484 3710 3862 5971 1493
Size: 1875 116 226 152 2109 1697
Fragments arranged by size:
2109 1875 1697 226 152 116
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 3500 1870
Size: 73 1557 4545
Fragments arranged by size:
4545 1557 73
AvrII C'CTAG_G
Cuts at: 2719 2719
Size: 6175
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 2357 3496 4951 618
Size: 325 217 627 512 58 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 512 325 217 58
BanII G_rGCy'C
Cuts at: 1391 2367 2719 3435 3494 1391
Size: 976 352 716 59 4072
Fragments arranged by size:
4072 976 716 352 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 928
Size: 971 130 5074
Fragments arranged by size:
5074 971 130
BbvCI CC'TCA_GC
Cuts at: 2046 2046
Size: 6175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2929 4067 5277 2929
Size: 1138 1210 3827
Fragments arranged by size:
3827 1210 1138
BclI T'GATC_A
Cuts at: 1548 2560 1548
Size: 1012 5163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2727 6170 2727
Size: 3443 2732
BlpI GC'TnA_GC
Cuts at: 2109 3065 3592 2109
Size: 956 527 4692
Fragments arranged by size:
4692 956 527
Bme1580I G_kGCm'C
Cuts at: 1790 2304 2719 3243 4236 5392 1790
Size: 514 415 524 993 1156 2573
Fragments arranged by size:
2573 1156 993 524 514 415
BmgBI CAC'GTC
Cuts at: 1318 2455 1318
Size: 1137 5038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3188 3906 4864
Size: 416 53 83 399 24 1771 718 958
Cuts at: 4864 442
Size: 1753
Fragments arranged by size:
1771 1753 958 718 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3032 3080 4835 1239
Size: 1793 48 1755 2579
Fragments arranged by size:
2579 1793 1755 48
Bpu10I CC'TnA_GC
Cuts at: 2046 2046
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 3966 493
Size: 711 1206 1556 2702
Fragments arranged by size:
2702 1556 1206 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3982 4364 4999 275
Size: 53 83 186 936 2449 382 635 1451
Fragments arranged by size:
2449 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3014 3044 3101 3131 3275
Size: 30 165 30 1611 30 57 30 144
Cuts at: 3275 3305 1178
Size: 30 4048
Fragments arranged by size:
4048 1611 165 144 57 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 2444 2756 3122 1186
Size: 1255 3 312 366 4239
Fragments arranged by size:
4239 1255 366 312 3
BseYI C'CCAG_C
Cuts at: 3434 3451 5378 5874 3434
Size: 17 1927 496 3735
Fragments arranged by size:
3735 1927 496 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2809 3350 1163
Size: 573 1073 541 3988
Fragments arranged by size:
3988 1073 573 541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2230 3625 4386 5414 1540
Size: 275 186 229 1395 761 1028 2301
Fragments arranged by size:
2301 1395 1028 761 275 229 186
BsiHKAI G_wGCw'C
Cuts at: 2418 4236 4321 5392 2418
Size: 1818 85 1071 3201
Fragments arranged by size:
3201 1818 1071 85
BsmI GAATG_Cn'
Cuts at: 3686 3779 3686
Size: 93 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2367 2418 2719 3243 3435 3494
Size: 399 514 63 51 301 524 192 59
Cuts at: 3494 4236 4321 5392 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 514 399 301 192
85 63 59 51
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 2665 2980 4064 5794 2665
Size: 315 1084 1730 3046
Fragments arranged by size:
3046 1730 1084 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2243 2451 3409 3530 844
Size: 1330 69 208 958 121 3489
Fragments arranged by size:
3489 1330 958 208 121 69
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 4062 1394
Size: 429 290 1949 3507
Fragments arranged by size:
3507 1949 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 3445 4670 4844 65
Size: 1183 2197 1225 174 1396
Fragments arranged by size:
2197 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 2327 1925
Size: 402 5773
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstXI CCAn_nnnn'nTGG
Cuts at: 2198 3133 3274 2198
Size: 935 141 5099
Fragments arranged by size:
5099 935 141
BstYI r'GATC_y
Cuts at: 1972 2727 3505 3872 4257 4274 5726 5812 5824
Size: 755 778 367 385 17 1452 86 12
Cuts at: 5824 6170 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 778 755 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2388 2580 513
Size: 552 1323 192 4108
Fragments arranged by size:
4108 1323 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2177 487
Size: 683 56 951 4485
Fragments arranged by size:
4485 951 683 56
BtsI GCAGTG_nn'
Cuts at: 2133 2210 2679 2836 3691 4485 4505 2133
Size: 77 469 157 855 794 20 3803
Fragments arranged by size:
3803 855 794 469 157 77 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2477 3186
Size: 54 1425 50 275 18 447 200 709
Cuts at: 3186 3426 3622 4511 6140 8
Size: 240 196 889 1629 43
Fragments arranged by size:
1629 1425 889 709 447 275 240 200
196 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2870 4105 1353
Size: 1517 1235 3423
Fragments arranged by size:
3423 1517 1235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3347 4762 5136 5282 387
Size: 1064 138 1758 1415 374 146 1280
Fragments arranged by size:
1758 1415 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2822 2873 3032 3080 4238 4835
Size: 393 455 735 51 159 48 1158 597
Cuts at: 4835 5622 5890 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1158 787 735 597 455 393 268
159 51 48
EcoO109I rG'GnC_Cy
Cuts at: 2440 2716 3082 2440
Size: 276 366 5533
Fragments arranged by size:
5533 366 276
EcoRI G'AATT_C
Cuts at: 3368 3484 3862 3368
Size: 116 378 5681
Fragments arranged by size:
5681 378 116
EcoRV GAT'ATC
Cuts at: 2098 2098
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373
Size: 32 135 32 3244 32 42 32 2626
Fragments arranged by size:
3244 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5289 5473 1305
Size: 3984 184 2007
Fragments arranged by size:
3984 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4353
Fragments arranged by size:
4353 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 2265 3050 3188 3233 3326 4133
Size: 648 1030 69 785 138 45 93 807
Cuts at: 4133 4492 4651 518
Size: 359 159 2042
Fragments arranged by size:
2042 1030 807 785 648 359 159 138
93 69 45
MspA1I CmG'CkG
Cuts at: 1606 2045 2800 3591 4268 5416 5661 1606
Size: 439 755 791 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 755 677 439 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5623
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 2506 2832 3055 3221 3355 3533 5078
Size: 97 1277 326 223 166 134 178 1545
Cuts at: 5078 1132
Size: 2229
Fragments arranged by size:
2229 1545 1277 326 223 178 166 134
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 2828 5074 2828
Size: 2246 3929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PmlI CAC'GTG
Cuts at: 2410 2410
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 4244 4276 5757 5789 3101
Size: 32 1111 32 1481 32 3487
Fragments arranged by size:
3487 1481 1111 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 3082 2440
Size: 642 5533
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PspOMI G'GGCC_C
Cuts at: 2715 2715
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2095 2577 3527 830
Size: 1265 482 950 3478
Fragments arranged by size:
3478 1265 950 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2800 2800
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SbfI CC_TGCA'GG
Cuts at: 2577 3527 2577
Size: 950 5225
ScaI AGT'ACT
Cuts at: 2474 2813 4423 6135 2474
Size: 339 1610 1712 2514
Fragments arranged by size:
2514 1712 1610 339
SfcI C'TryA_G
Cuts at: 826 1045 2091 2573 3523 4658 5339 5530 826
Size: 219 1046 482 950 1135 681 191 1471
Fragments arranged by size:
1471 1135 1046 950 681 482 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4279 5180 5442 820
Size: 197 926 2336 901 262 1553
Fragments arranged by size:
2336 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 2506 3221 3533 1132
Size: 1374 715 312 3774
Fragments arranged by size:
3774 1374 715 312
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 2571 3101 3113 1517
Size: 1054 530 12 4579
Fragments arranged by size:
4579 1054 530 12
StyI C'CwwG_G
Cuts at: 513 1065 2076 2719 2931 3614 513
Size: 552 1011 643 212 683 3074
Fragments arranged by size:
3074 1011 683 643 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4202 4219 4372 4557 5002 325
Size: 1255 2622 17 153 185 445 1498
Fragments arranged by size:
2622 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2508 2639
Size: 274 80 33 51 1443 495 36 131
Cuts at: 2639 2811 3375 4421 6133 96
Size: 172 564 1046 1712 138
Fragments arranged by size:
1712 1443 1046 564 495 274 172 138
131 80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 6135 6167 3275
Size: 32 2828 32 3283
Fragments arranged by size:
3283 2828 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2752 1316
Size: 1436 4739
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2938 3133 3421 2608
Size: 330 195 288 5362
Fragments arranged by size:
5362 330 288 195
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 3368 4304 3368
Size: 936 5239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI