Restriction map for
pFN21AB5856
(Circular) (Six-base) MAPSORT of: pFN21AB5856.seq Check: 1168 from: 1 to: 6172
pFN21AB5856 6172 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 3099 4235 5619 5887 1632
Size: 801 666 1136 1384 268 1917
Fragments arranged by size:
1917 1384 1136 801 666 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2668 3385 5071 6048 1203
Size: 1465 717 1686 977 1327
Fragments arranged by size:
1686 1465 1327 977 717
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 3915 5487 3915
Size: 1572 4600
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 2212 2290 2357 2632 3290 3464 3481 3707
Size: 719 78 67 275 658 174 17 226
Cuts at: 3707 3859 5968 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 719 658 275 226 174 152
78 67 17
AseI AT'TA_AT
Cuts at: 160 1032 2823 4726 160
Size: 872 1791 1903 1606
Fragments arranged by size:
1903 1791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 3103 3497 1870
Size: 73 1160 394 4545
Fragments arranged by size:
4545 1160 394 73
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 3493 4948 618
Size: 325 217 627 276 1430 1455 1842
Fragments arranged by size:
1842 1455 1430 627 325 276 217
BanII G_rGCy'C
Cuts at: 1391 3339 3491 1391
Size: 1948 152 4072
Fragments arranged by size:
4072 1948 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3021 928
Size: 971 1122 4079
Fragments arranged by size:
4079 1122 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2632 4064 5274 2632
Size: 1432 1210 3530
Fragments arranged by size:
3530 1432 1210
BclI T'GATC_A
Cuts at: 1548 2269 3220 1548
Size: 721 951 4500
Fragments arranged by size:
4500 951 721
BfrBI ATG'CAT
Cuts at: 3007 3007
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4783 136
Size: 107 122 71 631 605 3111 1525
Fragments arranged by size:
3111 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 2786 3589 2786
Size: 803 5369
Bme1580I G_kGCm'C
Cuts at: 1790 2066 4233 5389 1790
Size: 276 2167 1156 2573
Fragments arranged by size:
2573 2167 1156 276
BmgBI CAC'GTC
Cuts at: 1318 2931 3097 1318
Size: 1613 166 4393
Fragments arranged by size:
4393 1613 166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2970 3184 3211 4832 1239
Size: 1731 214 27 1621 2579
Fragments arranged by size:
2579 1731 1621 214 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 2487 6166 2487
Size: 3679 2493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3979 4361 4996 275
Size: 53 83 186 936 2446 382 635 1451
Fragments arranged by size:
2446 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4605 5003 5277 5424 5835 4605
Size: 398 274 147 411 4942
Fragments arranged by size:
4942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5947
Fragments arranged by size:
5947 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 2429 1186
Size: 817 426 4929
Fragments arranged by size:
4929 817 426
BseYI C'CCAG_C
Cuts at: 5375 5871 5375
Size: 496 5676
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5599
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3622 4383 5411 1540
Size: 275 186 1621 761 1028 2301
Fragments arranged by size:
2301 1621 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3339 4233 4318 5389 3339
Size: 894 85 1071 4122
Fragments arranged by size:
4122 1071 894 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
BsmBI CGTCTCn'nnnn_
Cuts at: 2460 2460
Size: 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 3339 3491 4233 4318 5389 1391
Size: 399 276 1273 152 742 85 1071 2174
Fragments arranged by size:
2174 1273 1071 742 399 276 152 85
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 2427 4061 5791 2427
Size: 1634 1730 2808
Fragments arranged by size:
2808 1730 1634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3527 844
Size: 2683 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4059 1394
Size: 429 2236 3507
Fragments arranged by size:
3507 2236 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4816 5003 1700
Size: 110 3006 187 2869
Fragments arranged by size:
3006 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2517 96
Size: 2421 3751
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6172
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 3259 3259
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 2071 2550 3397 3502 3869 4254 4271 5723
Size: 99 479 847 105 367 385 17 1452
Cuts at: 5723 5809 5821 6167 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 847 479 385 367 346 105
99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 513
Size: 552 939 63 4618
Fragments arranged by size:
4618 939 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3163 487
Size: 683 56 1937 3496
Fragments arranged by size:
3496 1937 683 56
BtsI GCAGTG_nn'
Cuts at: 3688 4482 4502 3688
Size: 794 20 5358
Fragments arranged by size:
5358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3349 3384 529
Size: 35 2785 35 3317
Fragments arranged by size:
3317 2785 35 35
DraI TTT'AAA
Cuts at: 3186 3476 3186
Size: 290 5882
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3619 4508 6137
Size: 54 1425 50 275 18 1789 889 1629
Cuts at: 6137 8
Size: 43
Fragments arranged by size:
1789 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2967 4102 1353
Size: 1614 1135 3423
Fragments arranged by size:
3423 1614 1135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3337 3337
Size: 6172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2970 3099 3184 3211 4235 4832
Size: 393 801 537 129 85 27 1024 597
Cuts at: 4832 5619 5887 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1024 801 787 597 537 393 268
129 85 27
EcoO109I rG'GnC_Cy
Cuts at: 2170 2170
Size: 6172
EcoRI G'AATT_C
Cuts at: 2212 2632 3481 3859 2212
Size: 420 849 378 4525
Fragments arranged by size:
4525 849 420 378
EcoRV GAT'ATC
Cuts at: 2404 2404
Size: 6172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2759 2904 2759
Size: 145 6027
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 5319 1678
Size: 3641 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2966 2998 3321 3353 4813
Size: 32 135 32 1394 32 323 32 1460
Cuts at: 4813 4845 4887 4919 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1460 1394 323 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 2112 3771 2112
Size: 1659 4513
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2302 5286 5470 1305
Size: 997 2984 184 2007
Fragments arranged by size:
2984 2007 997 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2277 2513 4130 4489 4648 518
Size: 648 1111 236 1617 359 159 2042
Fragments arranged by size:
2042 1617 1111 648 359 236 159
MspA1I CmG'CkG
Cuts at: 1606 2069 3255 3588 4265 5413 5658 1606
Size: 463 1186 333 677 1148 245 2120
Fragments arranged by size:
2120 1186 1148 677 463 333 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4681
Fragments arranged by size:
4681 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NsiI A_TGCA'T
Cuts at: 3009 3009
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2672 3007 3389 3530 5075 1132
Size: 97 1443 335 382 141 1545 2229
Fragments arranged by size:
2229 1545 1443 382 335 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 2668 3385 5071 2668
Size: 717 1686 3769
Fragments arranged by size:
3769 1686 717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PfoI T'CCnGG_A
Cuts at: 3022 3022
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3153 4241 4273 5754 5786 3121
Size: 32 1088 32 1481 32 3507
Fragments arranged by size:
3507 1481 1088 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2170
Size: 6172
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2722 2754 2722
Size: 32 6140
PstI C_TGCA'G
Cuts at: 830 3524 830
Size: 2694 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 3255 3255
Size: 6172
SacI G_AGCT'C
Cuts at: 3339 3339
Size: 6172
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SapI GCTCTTCn'nnn_
Cuts at: 2967 2967
Size: 6172
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 2642 4420 6132 2642
Size: 1778 1712 2682
Fragments arranged by size:
2682 1778 1712
SfcI C'TryA_G
Cuts at: 826 1045 3520 4655 5336 5527 826
Size: 219 2475 1135 681 191 1471
Fragments arranged by size:
2475 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 3105 3499 1872
Size: 1233 394 4545
Fragments arranged by size:
4545 1233 394
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3007 3530 1132
Size: 1875 523 3774
Fragments arranged by size:
3774 1875 523
SspI AAT'ATT
Cuts at: 5 52 2242 2263 2850 3088 4096 5
Size: 47 2190 21 587 238 1008 2081
Fragments arranged by size:
2190 2081 1008 587 238 47 21
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6172
StyI C'CwwG_G
Cuts at: 513 1065 2004 3611 513
Size: 552 939 1607 3074
Fragments arranged by size:
3074 1607 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4199 4216 4369 4554 4999 325
Size: 1255 2619 17 153 185 445 1498
Fragments arranged by size:
2619 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 2640 4418
Size: 274 80 33 51 1443 540 123 1778
Cuts at: 4418 6130 96
Size: 1712 138
Fragments arranged by size:
1778 1712 1443 540 274 138 123 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 3098 1316
Size: 1782 4390
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3167 3167
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 3103 3497 1870
Size: 1233 394 4545
Fragments arranged by size:
4545 1233 394
XmnI GAAnn'nnTTC
Cuts at: 3087 3208 4301 3087
Size: 121 1093 4958
Fragments arranged by size:
4958 1093 121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI