Restriction map for
pFN21AB5855
(Circular) (Six-base) MAPSORT of: pFN21AB5855.seq Check: 344 from: 1 to: 6082
pFN21AB5855 6082 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3098 3403 3098
Size: 305 5777
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2192 2543 2783 4145 5529 5797 1632
Size: 447 113 351 240 1362 1384 268 1917
Fragments arranged by size:
1917 1384 1362 447 351 268 240 113
AfeI AGC'GCT
Cuts at: 3269 3269
Size: 6082
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 2201 2949 1166
Size: 1035 748 4299
Fragments arranged by size:
4299 1035 748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2357 2826 1184
Size: 1173 469 4440
Fragments arranged by size:
4440 1173 469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3274 3306 3274
Size: 32 6050
AlwNI CAG_nnn'CTG
Cuts at: 2305 2651 3118 3825 5397 2305
Size: 346 467 707 1572 2990
Fragments arranged by size:
2990 1572 707 467 346
ApaI G_GGCC'C
Cuts at: 3138 3231 3138
Size: 93 5989
ApaLI G'TGCA_C
Cuts at: 4139 5295 4139
Size: 1156 4926
ApoI r'AATT_y
Cuts at: 1493 2373 3391 3617 3769 5878 1493
Size: 880 1018 226 152 2109 1697
Fragments arranged by size:
2109 1697 1018 880 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 2262 3246 3312 3407 1870
Size: 73 319 984 66 95 4545
Fragments arranged by size:
4545 984 319 95 73 66
BamHI G'GATC_C
Cuts at: 2852 3412 3779 2852
Size: 560 367 5155
Fragments arranged by size:
5155 560 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3063 3098 3403 4858
Size: 325 217 627 449 827 35 305 1455
Cuts at: 4858 618
Size: 1842
Fragments arranged by size:
1842 1455 827 627 449 325 305 217
35
BanII G_rGCy'C
Cuts at: 1391 2616 3138 3150 3231 3401 1391
Size: 1225 522 12 81 170 4072
Fragments arranged by size:
4072 1225 522 170 81 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2235 3974 5184 2235
Size: 1739 1210 3133
Fragments arranged by size:
3133 1739 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4693 136
Size: 107 122 71 631 605 337 2684 1525
Fragments arranged by size:
2684 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2136 2244 6077 2136
Size: 108 3833 2141
Fragments arranged by size:
3833 2141 108
BlpI GC'TnA_GC
Cuts at: 2772 3499 2772
Size: 727 5355
Bme1580I G_kGCm'C
Cuts at: 1790 2336 3138 3231 4143 5299 1790
Size: 546 802 93 912 1156 2573
Fragments arranged by size:
2573 1156 912 802 546 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2679 3024
Size: 416 53 83 399 24 636 626 345
Cuts at: 3024 3813 4771 442
Size: 789 958 1753
Fragments arranged by size:
1753 958 789 636 626 416 399 345
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2633
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4742 1239
Size: 3503 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3125 4207 5072 5370 2786
Size: 339 1082 865 298 3498
Fragments arranged by size:
3498 1082 865 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4745 882
Size: 617 3246 2219
Fragments arranged by size:
3246 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2314 3873 493
Size: 711 1110 1559 2702
Fragments arranged by size:
2702 1559 1110 711
BsaBI GATnn'nnATC
Cuts at: 2141 2249 6076 2141
Size: 108 3827 2147
Fragments arranged by size:
3827 2147 108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3889 4271 4906
Size: 53 83 186 936 479 1877 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1877 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5857
Fragments arranged by size:
5857 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2913 1186
Size: 865 862 4355
Fragments arranged by size:
4355 865 862
BseYI C'CCAG_C
Cuts at: 2215 2305 2324 2329 2864 2940 3261 5285 5781
Size: 90 19 5 535 76 321 2024 496
Cuts at: 5781 2215
Size: 2516
Fragments arranged by size:
2516 2024 535 496 321 90 76 19
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 2626 2709 1163
Size: 573 876 14 83 4536
Fragments arranged by size:
4536 876 573 83 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4143 4228 5299 4143
Size: 85 1071 4926
Fragments arranged by size:
4926 1071 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2336 2616 3138 3150 3231 3401 4143
Size: 399 546 280 522 12 81 170 742
Cuts at: 4143 4228 5299 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 522 399 280 170
85 81 12
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3437 844
Size: 2593 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2746 3969 1394
Size: 429 923 1223 3507
Fragments arranged by size:
3507 1223 923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3330 4726 4913 1700
Size: 110 1520 1396 187 2869
Fragments arranged by size:
2869 1520 1396 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 2618 4142 5154 2618
Size: 1524 1012 3546
Fragments arranged by size:
3546 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6082
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2136 2244 2283 2852 3016 3412 3779 4164
Size: 164 108 39 569 164 396 367 385
Cuts at: 4164 4181 5633 5719 5731 6077 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 569 396 385 367 346 164
164 108 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3129 3129
Size: 6082
BtgI C'CryG_G
Cuts at: 513 1065 2004 2129 2667 3150 513
Size: 552 939 125 538 483 3445
Fragments arranged by size:
3445 939 552 538 483 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 2334 2476 2482 2600 3598 4392 4412 2160
Size: 174 142 6 118 998 794 20 3830
Fragments arranged by size:
3830 998 794 174 142 118 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2401 3529
Size: 54 1425 50 275 18 486 85 1128
Cuts at: 3529 4418 6047 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 486 275 85 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4012 1353
Size: 2659 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4669 5043 5189 387
Size: 1064 138 3080 374 146 1280
Fragments arranged by size:
3080 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2192 2543 2783 4145 4742 5529
Size: 393 447 113 351 240 1362 597 787
Cuts at: 5529 5797 1239
Size: 268 1524
Fragments arranged by size:
1524 1362 787 597 447 393 351 268
240 113
EcoO109I rG'GnC_Cy
Cuts at: 2047 2212 3038 3134 3135 3191 3209 3227 3228
Size: 165 826 96 1 56 18 18 1
Cuts at: 3228 2047
Size: 4901
Fragments arranged by size:
4901 826 165 96 56 18 18 1
1
EcoRI G'AATT_C
Cuts at: 3391 3769 3391
Size: 378 5704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 6082
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 3271 5229 1678
Size: 1593 1958 2531
Fragments arranged by size:
2531 1958 1593
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 2775 2807 4723
Size: 32 135 32 1046 32 125 32 1916
Cuts at: 4723 4755 4797 4829 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1916 1046 135 125 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 3672 669
Size: 988 281 1488 246 3079
Fragments arranged by size:
3079 1488 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3102 3407 3102
Size: 305 5777
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 2403 10
Size: 54 1425 343 486 85 3689
Fragments arranged by size:
3689 1425 486 343 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2339 2949 4040 4399 4558 518
Size: 648 1035 138 610 1091 359 159 2042
Fragments arranged by size:
2042 1091 1035 648 610 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2027 2834 3219 3498 4175 5323 5568 1606
Size: 421 807 385 279 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 421 385 279 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2129 2667 3150 513
Size: 552 939 125 538 483 3445
Fragments arranged by size:
3445 939 552 538 483 125
NdeI CA'TA_TG
Cuts at: 387 2948 387
Size: 2561 3521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 2564 3440 4985 1132
Size: 97 1335 876 1545 2229
Fragments arranged by size:
2229 1545 1335 876 97
PasI CC'CwG_GG
Cuts at: 1348 3355 1348
Size: 2007 4075
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2509 1108
Size: 1401 4681
PfoI T'CCnGG_A
Cuts at: 2430 2430
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PmlI CAC'GTG
Cuts at: 2314 2314
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2212 3038 3191 2047
Size: 165 826 153 4938
Fragments arranged by size:
4938 826 165 153
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PspOMI G'GGCC_C
Cuts at: 3134 3227 3134
Size: 93 5989
PstI C_TGCA'G
Cuts at: 830 2026 2032 2167 3434 830
Size: 1196 6 135 1267 3478
Fragments arranged by size:
3478 1267 1196 135 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2027 2834 3219 2027
Size: 807 385 4890
Fragments arranged by size:
4890 807 385
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SexAI A'CCwGG_T
Cuts at: 2969 2969
Size: 6082
SfcI C'TryA_G
Cuts at: 826 1045 2022 2028 2096 2163 2835 3430 4565
Size: 219 977 6 68 67 672 595 1135
Cuts at: 4565 5246 5437 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 977 681 672 595 219 191
68 67 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2765 3140 4186 5087 5349 820
Size: 197 926 822 375 1046 901 262 1553
Fragments arranged by size:
1553 1046 926 901 822 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 2564 3440 1132
Size: 1432 876 3774
Fragments arranged by size:
3774 1432 876
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 2444 1517
Size: 927 5155
StyI C'CwwG_G
Cuts at: 513 1065 2004 2129 2319 2667 3150 3178 3521
Size: 552 939 125 190 348 483 28 343
Cuts at: 3521 513
Size: 3074
Fragments arranged by size:
3074 939 552 483 348 343 190 125
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 3081 3125 4109 4126 4279 4464
Size: 1255 1298 203 44 984 17 153 185
Cuts at: 4464 4909 325
Size: 445 1498
Fragments arranged by size:
1498 1298 1255 984 445 203 185 153
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4328 6040 96
Size: 274 80 33 51 1443 2351 1712 138
Fragments arranged by size:
2351 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 2886 2918 6042 6074 2137
Size: 32 717 32 3124 32 2145
Fragments arranged by size:
3124 2145 717 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2045 2971 1316
Size: 729 926 4427
Fragments arranged by size:
4427 926 729
XbaI T'CTAG_A
Cuts at: 2086 3418 2086
Size: 1332 4750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 2326
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 4211 4211
Size: 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI