Restriction map for
pFN21AB5852
(Circular) (Six-base) MAPSORT of: pFN21AB5852.seq Check: 7517 from: 1 to: 6112
pFN21AB5852 6112 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2094 3922 278
Size: 53 83 186 936 558 1828 2468
Fragments arranged by size:
2468 1828 936 558 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2851 3455 1937
Size: 914 604 4594
Fragments arranged by size:
4594 914 604
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2655 4175 5559 5827 1632
Size: 1023 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 1023 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 2328 4943 2328
Size: 2615 3497
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 3855 5427 3855
Size: 1572 4540
ApaLI G'TGCA_C
Cuts at: 2600 4169 5325 2600
Size: 1569 1156 3387
Fragments arranged by size:
3387 1569 1156
ApoI r'AATT_y
Cuts at: 1493 2124 3050 3065 3079 3421 3647 3799 5908
Size: 631 926 15 14 342 226 152 2109
Cuts at: 5908 1493
Size: 1697
Fragments arranged by size:
2109 1697 926 631 342 226 152 15
14
AseI AT'TA_AT
Cuts at: 160 1032 2255 4666 160
Size: 872 1223 2411 1606
Fragments arranged by size:
2411 1606 1223 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 3437 1870
Size: 73 1494 4545
Fragments arranged by size:
4545 1494 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2318 3433 4888 618
Size: 325 217 627 531 1115 1455 1842
Fragments arranged by size:
1842 1455 1115 627 531 325 217
BanII G_rGCy'C
Cuts at: 1391 2439 3184 3431 1391
Size: 1048 745 247 4072
Fragments arranged by size:
4072 1048 745 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2094 2164 928
Size: 971 195 70 4876
Fragments arranged by size:
4876 971 195 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2604 4173 5329 1790
Size: 814 1569 1156 2573
Fragments arranged by size:
2573 1569 1156 814
BmgBI CAC'GTC
Cuts at: 1318 2599 3378 1318
Size: 1281 779 4052
Fragments arranged by size:
4052 1281 779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3020 3180 3843
Size: 416 53 83 399 24 1603 160 663
Cuts at: 3843 4801 442
Size: 958 1753
Fragments arranged by size:
1753 1603 958 663 416 399 160 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 4772 1239
Size: 1214 2319 2579
Fragments arranged by size:
2579 2319 1214
Bpu10I CC'TnA_GC
Cuts at: 3270 3270
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2972 3124 4237 5102 5400 2291
Size: 681 152 1113 865 298 3003
Fragments arranged by size:
3003 1113 865 681 298 152
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 2817 6106 2817
Size: 3289 2823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 3919 4301 4936
Size: 53 83 186 936 558 1828 382 635
Cuts at: 4936 275
Size: 1451
Fragments arranged by size:
1828 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2328 4545 4943 5217 5364 5775 2328
Size: 2217 398 274 147 411 2665
Fragments arranged by size:
2665 2217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2743 2773 1178
Size: 30 165 30 1340 30 4517
Fragments arranged by size:
4517 1340 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6112
BseYI C'CCAG_C
Cuts at: 5315 5811 5315
Size: 496 5616
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5539
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2604 3279 4173 4258 5329 2439
Size: 165 675 894 85 1071 3222
Fragments arranged by size:
3222 1071 894 675 165 85
BsiWI C'GTAC_G
Cuts at: 2135 2135
Size: 6112
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2604 3184 3279 3431 4173 4258
Size: 399 649 165 580 95 152 742 85
Cuts at: 4258 5329 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 649 580 399 165 152
95 85
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 4756 4943 1700
Size: 110 518 2428 187 2869
Fragments arranged by size:
2869 2428 518 187 110
BsrGI T'GTAC_A
Cuts at: 96 2073 96
Size: 1977 4135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2068
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2224 2565 3323 3442 3809 4194 4211 5663
Size: 252 341 758 119 367 385 17 1452
Cuts at: 5663 5749 5761 6107 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 341 252
119 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2266 2918 513
Size: 552 939 262 652 3707
Fragments arranged by size:
3707 939 652 552 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 3105 3628 4422 4442 3105
Size: 523 794 20 4775
Fragments arranged by size:
4775 794 523 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2619 2654 529
Size: 35 2055 35 3987
Fragments arranged by size:
3987 2055 35 35
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2351 2463
Size: 54 1425 50 275 18 216 305 112
Cuts at: 2463 3559 4448 6077 8
Size: 1096 889 1629 43
Fragments arranged by size:
1629 1425 1096 889 305 275 216 112
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 4042 1353
Size: 1184 1505 3423
Fragments arranged by size:
3423 1505 1184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 6112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2655 4175 4772 5559 5827 1239
Size: 393 821 202 1520 597 787 268 1524
Fragments arranged by size:
1524 1520 821 787 597 393 268 202
EcoO109I rG'GnC_Cy
Cuts at: 3106 3106
Size: 6112
EcoRI G'AATT_C
Cuts at: 2124 3065 3421 3799 2124
Size: 941 356 378 4437
Fragments arranged by size:
4437 941 378 356
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 2240 5259 1678
Size: 562 3019 2531
Fragments arranged by size:
3019 2531 562
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2421 2453 4753 4785 4827
Size: 32 135 32 849 32 2300 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 2300 849 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3390 3456 3702 669
Size: 988 281 1452 66 246 3079
Fragments arranged by size:
3079 1452 988 281 246 66
HindIII A'AGCT_T
Cuts at: 748 2146 748
Size: 1398 4714
HpaI GTT'AAC
Cuts at: 3390 3702 3390
Size: 312 5800
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2786 5226 5410 1305
Size: 1481 2440 184 2007
Fragments arranged by size:
2440 2007 1481 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2353 2465 10
Size: 54 1425 343 521 112 3657
Fragments arranged by size:
3657 1425 521 343 112 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2507 4070 4429 4588 518
Size: 648 903 438 1563 359 159 2042
Fragments arranged by size:
2042 1563 903 648 438 359 159
MspA1I CmG'CkG
Cuts at: 1606 3528 4205 5353 5598 1606
Size: 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2918 513
Size: 552 939 914 3707
Fragments arranged by size:
3707 939 914 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2068 3470 5015 1132
Size: 97 839 1402 1545 2229
Fragments arranged by size:
2229 1545 1402 839 97
PasI CC'CwG_GG
Cuts at: 1348 2011 1348
Size: 663 5449
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2943 1108
Size: 1835 4277
PfoI T'CCnGG_A
Cuts at: 3175 3175
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2573 2605 4181 4213 5694 5726 2573
Size: 32 1576 32 1481 32 2959
Fragments arranged by size:
2959 1576 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3106 3106
Size: 6112
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3320 3682 3320
Size: 362 5750
PstI C_TGCA'G
Cuts at: 830 2475 3464 830
Size: 1645 989 3478
Fragments arranged by size:
3478 1645 989
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2217 2471 3347 3460 4595 5276 5467
Size: 219 1172 254 876 113 1135 681 191
Cuts at: 5467 826
Size: 1471
Fragments arranged by size:
1471 1172 1135 876 681 254 219 191
113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2951 3139 4216 5117 5379
Size: 197 926 363 645 188 1077 901 262
Cuts at: 5379 820
Size: 1553
Fragments arranged by size:
1553 1077 926 901 645 363 262 197
188
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 2795 4036 5
Size: 47 2743 1241 2081
Fragments arranged by size:
2743 2081 1241 47
StuI AGG'CCT
Cuts at: 1517 3121 1517
Size: 1604 4508
StyI C'CwwG_G
Cuts at: 513 1065 2004 2918 3551 513
Size: 552 939 914 633 3074
Fragments arranged by size:
3074 939 914 633 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2074 2755 4139 4156 4309 4494 4939
Size: 1255 494 681 1384 17 153 185 445
Cuts at: 4939 325
Size: 1498
Fragments arranged by size:
1498 1384 1255 681 494 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 4358 6070
Size: 274 80 33 51 1443 96 2285 1712
Cuts at: 6070 96
Size: 138
Fragments arranged by size:
2285 1712 1443 274 138 96 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3055 3448 3055
Size: 393 5719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2361 2041
Size: 320 5792
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2092 3920 276
Size: 53 83 186 936 558 1828 2468
Fragments arranged by size:
2468 1828 936 558 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI