Restriction map for
pFN21AB5849
(Circular) (Six-base) MAPSORT of: pFN21AB5849.seq Check: 3027 from: 1 to: 5443
pFN21AB5849 5443 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3506 4890 5158 1632
Size: 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1384 268
AfeI AGC'GCT
Cuts at: 2642 2642
Size: 5443
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 2288 1166
Size: 1122 4321
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2089 2186 3186 4758 2089
Size: 97 1000 1572 2774
Fragments arranged by size:
2774 1572 1000 97
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2509 2600 2768 1870
Size: 73 566 91 168 4545
Fragments arranged by size:
4545 566 168 91 73
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 2764 4219 618
Size: 325 217 627 786 191 1455 1842
Fragments arranged by size:
1842 1455 786 627 325 217 191
BanII G_rGCy'C
Cuts at: 1391 2453 2592 2601 2607 2762 1391
Size: 1062 139 9 6 155 4072
Fragments arranged by size:
4072 1062 155 139 9 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BbvCI CC'TCA_GC
Cuts at: 2259 2259
Size: 5443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 2292 1548
Size: 744 4699
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2576 3504 4660 1790
Size: 786 928 1156 2573
Fragments arranged by size:
2573 1156 928 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 3174 4132
Size: 416 53 83 399 24 903 854 958
Cuts at: 4132 442
Size: 1753
Fragments arranged by size:
1753 958 903 854 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2114 4103 1239
Size: 824 51 1989 2579
Fragments arranged by size:
2579 1989 824 51
Bpu10I CC'TnA_GC
Cuts at: 2259 2259
Size: 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2307 3876 4274 4548 4695 5106 2307
Size: 1569 398 274 147 411 2644
Fragments arranged by size:
2644 1569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2459 1186
Size: 1062 211 4170
Fragments arranged by size:
4170 1062 211
BseYI C'CCAG_C
Cuts at: 2135 2411 4646 5142 2135
Size: 276 2235 496 2436
Fragments arranged by size:
2436 2235 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4870
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 2893 3654 4682 1540
Size: 275 186 307 585 761 1028 2301
Fragments arranged by size:
2301 1028 761 585 307 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2408 2954 3047 2408
Size: 546 93 4804
Fragments arranged by size:
4804 546 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2576 2592 2601 2607 2762 3504
Size: 399 663 123 16 9 6 155 742
Cuts at: 3504 3589 4660 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 399 155 123 85
16 9 6
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 2484 96
Size: 2388 3055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 4148
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 2458 1184
Size: 1274 4169
BstYI r'GATC_y
Cuts at: 1972 2534 2715 2773 3140 3525 3542 4994 5080
Size: 562 181 58 367 385 17 1452 86
Cuts at: 5080 5092 5438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 562 385 367 346 181 86
58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2436 2436
Size: 5443
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4891
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 487
Size: 683 56 935 3769
Fragments arranged by size:
3769 935 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 2552 2959 3753 3773 2090
Size: 462 407 794 20 3760
Fragments arranged by size:
3760 794 462 407 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2890 3779
Size: 54 1425 50 275 18 219 841 889
Cuts at: 3779 5408 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 841 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2225 3373 1353
Size: 740 132 1148 3423
Fragments arranged by size:
3423 1148 740 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2645 4030 4404 4550 387
Size: 1064 138 1056 1385 374 146 1280
Fragments arranged by size:
1385 1280 1064 1056 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2114 3506 4103 4890 5158 1239
Size: 393 431 51 1392 597 787 268 1524
Fragments arranged by size:
1524 1392 787 597 431 393 268 51
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 2375 3949 2375
Size: 1574 3869
HaeII r_GCGC'y
Cuts at: 1678 2644 4590 1678
Size: 966 1946 2531
Fragments arranged by size:
2531 1946 966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2526 2558 2576 2608 4084
Size: 32 135 32 954 32 18 32 1476
Cuts at: 4084 4116 4158 4190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1476 954 135 42 32 32 32
32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2304 4557 4741 1305
Size: 999 2253 184 2007
Fragments arranged by size:
2253 2007 999 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 10
Size: 54 1425 343 219 3402
Fragments arranged by size:
3402 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 3401 3760 3919 518
Size: 648 1122 1113 359 159 2042
Fragments arranged by size:
2042 1122 1113 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 2431 2859 3536 4684 4929 1606
Size: 615 210 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 428 245 210
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 2287 387
Size: 1900 3543
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2638 2801 4346 1132
Size: 97 1409 163 1545 2229
Fragments arranged by size:
2229 1545 1409 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2534 2559 2795 830
Size: 1704 25 236 3478
Fragments arranged by size:
3478 1704 236 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2530 2555 2791 3926 4607 4798 826
Size: 219 1485 25 236 1135 681 191 1471
Fragments arranged by size:
1485 1471 1135 681 236 219 191 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2511 2770 1872
Size: 639 259 4545
Fragments arranged by size:
4545 639 259
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 2675 1517
Size: 1158 4285
StyI C'CwwG_G
Cuts at: 513 1065 2882 513
Size: 552 1817 3074
Fragments arranged by size:
3074 1817 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2515 3470 3487 3640 3825 4270
Size: 1255 591 344 955 17 153 185 445
Cuts at: 4270 325
Size: 1498
Fragments arranged by size:
1498 1255 955 591 445 344 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3689 5401
Size: 274 80 33 51 1443 507 1205 1712
Cuts at: 5401 96
Size: 138
Fragments arranged by size:
1712 1443 1205 507 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2509 2768 1870
Size: 639 259 4545
Fragments arranged by size:
4545 639 259
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI