Restriction map for
pFN21AB5848
(Circular) (Six-base) MAPSORT of: pFN21AB5848.seq Check: 6804 from: 1 to: 6163
pFN21AB5848 6163 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2242 3249 3506 1937
Size: 305 1007 257 4594
Fragments arranged by size:
4594 1007 305 257
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 4226 5610 5878 1632
Size: 1013 1581 1384 268 1917
Fragments arranged by size:
1917 1581 1384 1013 268
AfeI AGC'GCT
Cuts at: 2114 2114
Size: 6163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2604 2636 2604
Size: 32 6131
AlwNI CAG_nnn'CTG
Cuts at: 2581 2902 3906 5478 2581
Size: 321 1004 1572 3266
Fragments arranged by size:
3266 1572 1004 321
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 2495 2564 2643 2654 2951 3174 3207 3472
Size: 1002 69 79 11 297 223 33 265
Cuts at: 3472 3698 3850 5959 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1002 297 265 226 223 152
79 69 33 11
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 3488 1870
Size: 73 1545 4545
Fragments arranged by size:
4545 1545 73
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 4939 618
Size: 325 217 627 1697 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2126 2522 3482 1391
Size: 735 396 960 4072
Fragments arranged by size:
4072 960 735 396
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 928
Size: 971 734 4458
Fragments arranged by size:
4458 971 734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3274 3308 4354 4388 1903
Size: 34 1337 34 1046 34 3678
Fragments arranged by size:
3678 1337 1046 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3094 4055 5265 3094
Size: 961 1210 3992
Fragments arranged by size:
3992 1210 961
BclI T'GATC_A
Cuts at: 1548 2382 2557 2934 1548
Size: 834 175 377 4777
Fragments arranged by size:
4777 834 377 175
BfrBI ATG'CAT
Cuts at: 2842 2842
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 4774 136
Size: 107 122 71 631 605 379 2723 1525
Fragments arranged by size:
2723 1525 631 605 379 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 2874 3580 2874
Size: 706 5457
Bme1580I G_kGCm'C
Cuts at: 1790 4224 5380 1790
Size: 2434 1156 2573
Fragments arranged by size:
2573 2434 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3894 4852 442
Size: 416 53 83 399 24 2477 958 1753
Fragments arranged by size:
2477 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2882 1056
Size: 1826 4337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 2924 4823 1239
Size: 1364 321 1899 2579
Fragments arranged by size:
2579 1899 1364 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2524 4288 5153 5451 2480
Size: 44 1764 865 298 3192
Fragments arranged by size:
3192 1764 865 298 44
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 2433 6157 2433
Size: 3724 2439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 4352 4987 275
Size: 53 83 186 936 2437 382 635 1451
Fragments arranged by size:
2437 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2395 2425 2562 2592 1178
Size: 30 165 30 992 30 137 30 4749
Fragments arranged by size:
4749 992 165 137 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3173 3395 1186
Size: 1987 222 3954
Fragments arranged by size:
3954 1987 222
BseYI C'CCAG_C
Cuts at: 2175 5366 5862 2175
Size: 3191 496 2476
Fragments arranged by size:
3191 2476 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2621 1163
Size: 573 411 474 4705
Fragments arranged by size:
4705 573 474 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4224 4309 5380 4224
Size: 85 1071 5007
Fragments arranged by size:
5007 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 3674 3767 2672
Size: 1002 93 5068
Fragments arranged by size:
5068 1002 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2522 3482 4224 4309 5380 1391
Size: 399 336 396 960 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 399 396 336 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 3518 844
Size: 2131 543 3489
Fragments arranged by size:
3489 2131 543
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 2786 4658 4832 65
Size: 1183 1290 248 1872 174 1396
Fragments arranged by size:
1872 1396 1290 1183 248 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 4807 4994 1700
Size: 110 213 2784 187 2869
Fragments arranged by size:
2869 2784 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 2893 3201 96
Size: 2797 308 3058
Fragments arranged by size:
3058 2797 308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 794
Size: 1376 4787
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2602 3493 3860 4245 4262 5714 5800 5812
Size: 630 891 367 385 17 1452 86 12
Cuts at: 5812 6158 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 891 630 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2243 3250 2243
Size: 1007 5156
Bsu36I CC'TnA_GG
Cuts at: 2705 2705
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 3679 4473 4493 2240
Size: 1439 794 20 3910
Fragments arranged by size:
3910 1439 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2957 3610 4499
Size: 54 1425 50 275 18 1127 653 889
Cuts at: 4499 6128 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1127 889 653 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 2436 3045 4093 1353
Size: 920 163 609 1048 3423
Fragments arranged by size:
3423 1048 920 609 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 2645 2924 4226 4823 5610 5878
Size: 393 971 42 279 1302 597 787 268
Cuts at: 5878 1239
Size: 1524
Fragments arranged by size:
1524 1302 971 787 597 393 279 268
42
EcoRI G'AATT_C
Cuts at: 2495 3472 3850 2495
Size: 977 378 4808
Fragments arranged by size:
4808 977 378
EcoRV GAT'ATC
Cuts at: 3072 3072
Size: 6163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3074 3074
Size: 6163
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2043 2116 5310 1678
Size: 365 73 3194 2531
Fragments arranged by size:
3194 2531 365 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2661 2693 4804 4836 4878
Size: 32 135 32 1089 32 2111 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 2111 1089 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3287 748
Size: 2539 3624
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3254 5277 5461 1305
Size: 1949 2023 184 2007
Fragments arranged by size:
2023 2007 1949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2959 10
Size: 54 1425 343 1127 3214
Fragments arranged by size:
3214 1425 1127 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 4121 4480 4639 518
Size: 648 1567 1388 359 159 2042
Fragments arranged by size:
2042 1567 1388 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 3579 4256 5404 5649 1606
Size: 564 1409 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 564 245
NaeI GCC'GGC
Cuts at: 2025 2025
Size: 6163
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5611
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 2023 2023
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 2878 1052
Size: 1826 4337
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NsiI A_TGCA'T
Cuts at: 2844 2844
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3433 3521 5066 1132
Size: 97 2204 88 1545 2229
Fragments arranged by size:
2229 2204 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 2119 1348
Size: 771 5392
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6163
PfoI T'CCnGG_A
Cuts at: 2580 2901 2580
Size: 321 5842
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PstI C_TGCA'G
Cuts at: 830 2972 3515 830
Size: 2142 543 3478
Fragments arranged by size:
3478 2142 543
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
RsrII CG'GwC_CG
Cuts at: 2089 2089
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SapI GCTCTTCn'nnn_
Cuts at: 2273 3045 2273
Size: 772 5391
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2968 3511 4646 5327 5518 826
Size: 219 1923 543 1135 681 191 1471
Fragments arranged by size:
1923 1471 1135 681 543 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 2023 1700
Size: 323 5840
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 2503 4267 5168 5430 820
Size: 197 926 516 44 1764 901 262 1553
Fragments arranged by size:
1764 1553 926 901 516 262 197 44
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 2760 4087 5
Size: 47 2708 1327 2081
Fragments arranged by size:
2708 2081 1327 47
StuI AGG'CCT
Cuts at: 1517 2465 1517
Size: 948 5215
StyI C'CwwG_G
Cuts at: 513 1065 3602 513
Size: 552 2537 3074
Fragments arranged by size:
3074 2537 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2077 4190 4207 4360 4545 4990 325
Size: 1255 497 2113 17 153 185 445 1498
Fragments arranged by size:
2113 1498 1255 497 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2893 3120 3201
Size: 274 80 33 51 1443 916 227 81
Cuts at: 3201 4409 6121 96
Size: 1208 1712 138
Fragments arranged by size:
1712 1443 1208 916 274 227 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6123 6155 6123
Size: 32 6131
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3403 3499 3403
Size: 96 6067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3241 3241
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6163
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 3141 4292 3141
Size: 1151 5012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NarI NruI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI