Restriction map for
pFN21AB5844
(Circular) (Six-base) MAPSORT of: pFN21AB5844.seq Check: 2571 from: 1 to: 6157
pFN21AB5844 6157 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2763 2763
Size: 6157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3967 278
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3500 1937
Size: 1563 4594
Acc65I G'GTAC_C
Cuts at: 3478 3478
Size: 6157
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2640 3431 4220 5604 5872 1632
Size: 764 244 791 789 1384 268 1917
Fragments arranged by size:
1917 1384 791 789 764 268 244
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5960
AflIII A'CryG_T
Cuts at: 1203 2445 3454 5056 6033 1203
Size: 1242 1009 1602 977 1327
Fragments arranged by size:
1602 1327 1242 1009 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 2370 2485 4886 2370
Size: 115 2401 3641
Fragments arranged by size:
3641 2401 115
AleI CACnn'nnGTG
Cuts at: 1166 3239 1166
Size: 2073 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3188 1184
Size: 2004 4153
AlwNI CAG_nnn'CTG
Cuts at: 3900 5472 3900
Size: 1572 4585
ApaI G_GGCC'C
Cuts at: 3025 3025
Size: 6157
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 2523 3119 3300 3368 3466 3692 3844 5953
Size: 1030 596 181 68 98 226 152 2109
Cuts at: 5953 1493
Size: 1697
Fragments arranged by size:
2109 1697 1030 596 226 181 152 98
68
AseI AT'TA_AT
Cuts at: 160 1032 4711 160
Size: 872 3679 1606
Fragments arranged by size:
3679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2258 2849 2951 3025 3482 1870
Size: 73 155 160 591 102 74 457 4545
Fragments arranged by size:
4545 591 457 160 155 102 74 73
BamHI G'GATC_C
Cuts at: 3487 3854 3487
Size: 367 5790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 3248 3478 4933 618
Size: 325 217 627 240 1221 230 1455 1842
Fragments arranged by size:
1842 1455 1221 627 325 240 230 217
BanII G_rGCy'C
Cuts at: 1391 2243 3025 3142 3476 1391
Size: 852 782 117 334 4072
Fragments arranged by size:
4072 852 782 334 117
BbeI G_GCGC'C
Cuts at: 2031 3252 2031
Size: 1221 4936
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5186
BbvCI CC'TCA_GC
Cuts at: 2991 2991
Size: 6157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2863 2897 4348 4382 1903
Size: 34 926 34 1451 34 3678
Fragments arranged by size:
3678 1451 926 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4049 5259 4049
Size: 1210 4947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 3162 4768
Size: 107 122 71 631 605 403 1087 1606
Cuts at: 4768 136
Size: 1525
Fragments arranged by size:
1606 1525 1087 631 605 403 122 107
71
BglII A'GATC_T
Cuts at: 6152 6152
Size: 6157
BlpI GC'TnA_GC
Cuts at: 3574 3574
Size: 6157
Bme1580I G_kGCm'C
Cuts at: 1790 3025 4218 5374 1790
Size: 1235 1193 1156 2573
Fragments arranged by size:
2573 1235 1193 1156
BmgBI CAC'GTC
Cuts at: 1318 3433 1318
Size: 2115 4042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2397 3888 4846
Size: 416 53 83 399 24 980 1491 958
Cuts at: 4846 442
Size: 1753
Fragments arranged by size:
1753 1491 980 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 6157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2582 2684 3345 4817 1239
Size: 905 438 102 661 1472 2579
Fragments arranged by size:
2579 1472 905 661 438 102
Bpu10I CC'TnA_GC
Cuts at: 2837 2991 2837
Size: 154 6003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3122 4282 5147 5445 3122
Size: 1160 865 298 3834
Fragments arranged by size:
3834 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2723 2927 3421 4820 882
Size: 617 1224 204 494 1399 2219
Fragments arranged by size:
2219 1399 1224 617 494 204
BsaAI yAC'GTr
Cuts at: 493 1204 2446 3948 493
Size: 711 1242 1502 2702
Fragments arranged by size:
2702 1502 1242 711
BsaBI GATnn'nnATC
Cuts at: 6151 6151
Size: 6157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3249 3964 4346
Size: 53 83 186 936 495 1221 715 382
Cuts at: 4346 4981 275
Size: 635 1451
Fragments arranged by size:
1451 1221 936 715 635 495 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3134 4590 4988 5262 5409 5820 3134
Size: 1456 398 274 147 411 3471
Fragments arranged by size:
3471 1456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5932
Fragments arranged by size:
5932 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2956 3278 1186
Size: 1770 322 4065
Fragments arranged by size:
4065 1770 322
BseYI C'CCAG_C
Cuts at: 2637 5360 5856 2637
Size: 2723 496 2938
Fragments arranged by size:
2938 2723 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2588 2599 1163
Size: 573 354 498 11 4721
Fragments arranged by size:
4721 573 498 354 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3607 4368 5396 1540
Size: 275 186 1018 588 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 588 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 4218 4303 5374 2265
Size: 1953 85 1071 3048
Fragments arranged by size:
3048 1953 1071 85
BsmI GAATG_Cn'
Cuts at: 3668 3761 3668
Size: 93 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2265 3025 3142 3476 4218 4303
Size: 399 453 22 760 117 334 742 85
Cuts at: 4303 5374 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 760 742 453 399 334 117
85 22
BspEI T'CCGG_A
Cuts at: 3134 5820 3134
Size: 2686 3471
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 3512 844
Size: 1919 749 3489
Fragments arranged by size:
3489 1919 749
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 4044 1394
Size: 429 335 1886 3507
Fragments arranged by size:
3507 1886 429 335
BsrDI GCAATG_nn'
Cuts at: 65 1248 4652 4826 65
Size: 1183 3404 174 1396
Fragments arranged by size:
3404 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2841 4801 4988 1700
Size: 110 1031 1960 187 2869
Fragments arranged by size:
2869 1960 1031 187 110
BsrGI T'GTAC_A
Cuts at: 96 2223 96
Size: 2127 4030
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 2566 794
Size: 1742 30 4385
Fragments arranged by size:
4385 1742 30
BstBI TT'CG_AA
Cuts at: 4961 4961
Size: 6157
BstEII G'GTnAC_C
Cuts at: 1184 2164 1184
Size: 980 5177
BstXI CCAn_nnnn'nTGG
Cuts at: 2409 2606 3241 2409
Size: 197 635 5325
Fragments arranged by size:
5325 635 197
BstYI r'GATC_y
Cuts at: 1972 2925 3487 3854 4239 4256 5708 5794 5806
Size: 953 562 367 385 17 1452 86 12
Cuts at: 5806 6152 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 953 562 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2939 3158 2939
Size: 219 5938
BtgI C'CryG_G
Cuts at: 513 1065 2004 3060 3234 513
Size: 552 939 1056 174 3436
Fragments arranged by size:
3436 1056 939 552 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5418
Fragments arranged by size:
5418 683 56
BtsI GCAGTG_nn'
Cuts at: 2479 2648 3673 4467 4487 2479
Size: 169 1025 794 20 4149
Fragments arranged by size:
4149 1025 794 169 20
ClaI AT'CG_AT
Cuts at: 1554 2745 1554
Size: 1191 4966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2785 2820 529
Size: 35 2221 35 3866
Fragments arranged by size:
3866 2221 35 35
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2312 5164 809
Size: 507 996 2852 1802
Fragments arranged by size:
2852 1802 996 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3063 3216 3604
Size: 54 1425 50 275 18 1233 153 388
Cuts at: 3604 4493 6122 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1233 889 388 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3604 1537
Size: 275 1792 4090
Fragments arranged by size:
4090 1792 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 2616 3309 4087 1353
Size: 1019 244 693 778 3423
Fragments arranged by size:
3423 1019 778 693 244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4744 5118 5264 387
Size: 1064 138 435 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2396 2582 2640 2684 3345 3431
Size: 393 512 252 186 58 44 661 86
Cuts at: 3431 4220 4817 5604 5872 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1524 789 787 661 597 512 393 268
252 186 86 58 44
EcoO109I rG'GnC_Cy
Cuts at: 2548 3022 2548
Size: 474 5683
EcoRI G'AATT_C
Cuts at: 2523 3368 3466 3844 2523
Size: 845 98 378 4836
Fragments arranged by size:
4836 845 378 98
FspI TGC'GCA
Cuts at: 4663 4663
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 2031 3252 5304 1678
Size: 353 1221 2052 2531
Fragments arranged by size:
2531 2052 1221 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 2471 2503 2940
Size: 32 135 32 784 32 83 32 437
Cuts at: 2940 2972 3097 3129 4798 4830 4872 4904 1373
Size: 32 125 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 784 437 135 125 83 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2364 2770 3309 3501 3747 669
Size: 988 281 426 406 539 192 246 3079
Fragments arranged by size:
3079 988 539 426 406 281 246 192
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6157
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KasI G'GCGC_C
Cuts at: 2027 3248 2027
Size: 1221 4936
KpnI G_GTAC'C
Cuts at: 3482 3482
Size: 6157
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 3315 5271 5455 1305
Size: 1515 495 1956 184 2007
Fragments arranged by size:
2007 1956 1515 495 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3218 10
Size: 54 1425 343 1386 2949
Fragments arranged by size:
2949 1425 1386 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2407 3239 4115 4474 4633 518
Size: 648 1241 832 876 359 159 2042
Fragments arranged by size:
2042 1241 876 832 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2995 3393 3573 4250 5398 5643 1606
Size: 1389 398 180 677 1148 245 2120
Fragments arranged by size:
2120 1389 1148 677 398 245 180
NaeI GCC'GGC
Cuts at: 2843 2843
Size: 6157
NarI GG'CG_CC
Cuts at: 2028 3249 2028
Size: 1221 4936
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4666
Fragments arranged by size:
4666 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6157
NgoMIV G'CCGG_C
Cuts at: 2841 2841
Size: 6157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 3604 3604
Size: 6157
NspI r_CATG'y
Cuts at: 1132 1229 2224 2536 2719 3515 5060 1132
Size: 97 995 312 183 796 1545 2229
Fragments arranged by size:
2229 1545 995 796 312 183 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6157
PciI A'CATG_T
Cuts at: 5056 5056
Size: 6157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6157
PfoI T'CCnGG_A
Cuts at: 2661 2928 2661
Size: 267 5890
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 5739 5771 4226
Size: 32 1481 32 4612
Fragments arranged by size:
4612 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2548
Size: 6157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6157
PsiI TTA'TAA
Cuts at: 3727 3727
Size: 6157
PspOMI G'GGCC_C
Cuts at: 3021 3021
Size: 6157
PstI C_TGCA'G
Cuts at: 830 2474 3509 830
Size: 1644 1035 3478
Fragments arranged by size:
3478 1644 1035
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6157
PvuII CAG'CTG
Cuts at: 3393 3393
Size: 6157
SalI G'TCGA_C
Cuts at: 1936 3499 1936
Size: 1563 4594
SapI GCTCTTCn'nnn_
Cuts at: 2616 2616
Size: 6157
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 2183 4405 6117 2183
Size: 2222 1712 2223
Fragments arranged by size:
2223 2222 1712
SfcI C'TryA_G
Cuts at: 826 1045 2470 3505 4640 5321 5512 826
Size: 219 1425 1035 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 1035 681 219 191
SfoI GGC'GCC
Cuts at: 2029 3250 2029
Size: 1221 4936
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 2100 3484 1872
Size: 228 1384 4545
Fragments arranged by size:
4545 1384 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 3101 4261 5162 5424 820
Size: 197 926 1008 150 1160 901 262 1553
Fragments arranged by size:
1553 1160 1008 926 901 262 197 150
SnaBI TAC'GTA
Cuts at: 493 3948 493
Size: 3455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6157
SphI G_CATG'C
Cuts at: 1132 3515 1132
Size: 2383 3774
SspI AAT'ATT
Cuts at: 5 52 4081 5
Size: 47 4029 2081
Fragments arranged by size:
4029 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6157
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3596 513
Size: 552 939 201 1391 3074
Fragments arranged by size:
3074 1391 939 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 3326 4184 4201 4354 4539 4984
Size: 1255 600 1146 858 17 153 185 445
Cuts at: 4984 325
Size: 1498
Fragments arranged by size:
1498 1255 1146 858 600 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2223 3387
Size: 274 80 33 51 1443 204 42 1164
Cuts at: 3387 4403 6115 96
Size: 1016 1712 138
Fragments arranged by size:
1712 1443 1164 1016 274 204 138 80
51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6117 6149 6117
Size: 32 6125
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6157
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XhoI C'TCGA_G
Cuts at: 1943 2951 1943
Size: 1008 5149
XmaI C'CCGG_G
Cuts at: 1870 2098 3482 1870
Size: 228 1384 4545
Fragments arranged by size:
4545 1384 228
XmnI GAAnn'nnTTC
Cuts at: 2465 4286 2465
Size: 1821 4336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3965 276
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI XcmI