Restriction map for
pFN21AB5842
(Circular) (Six-base) MAPSORT of: pFN21AB5842.seq Check: 8656 from: 1 to: 6154
pFN21AB5842 6154 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2942 2984 4217 5601 5869 1632
Size: 872 438 42 1233 1384 268 1917
Fragments arranged by size:
1917 1384 1233 872 438 268 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AlwNI CAG_nnn'CTG
Cuts at: 3897 5469 3897
Size: 1572 4582
ApaLI G'TGCA_C
Cuts at: 2651 4211 5367 2651
Size: 1560 1156 3438
Fragments arranged by size:
3438 1560 1156
ApoI r'AATT_y
Cuts at: 1493 2334 2550 2869 3463 3689 3841 5950 1493
Size: 841 216 319 594 226 152 2109 1697
Fragments arranged by size:
2109 1697 841 594 319 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 3189 3479 1870
Size: 73 1246 290 4545
Fragments arranged by size:
4545 1246 290 73
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 3475 4930 618
Size: 325 217 627 660 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 660 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3473 1391
Size: 2082 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3101 3135 4345 4379 1903
Size: 34 1164 34 1210 34 3678
Fragments arranged by size:
3678 1210 1164 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 2716 3315 1548
Size: 1168 599 4387
Fragments arranged by size:
4387 1168 599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4765 136
Size: 107 122 71 631 605 3093 1525
Fragments arranged by size:
3093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3301 3571 3301
Size: 270 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2111 2655 4215 5371 1790
Size: 321 544 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 544 321
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2929 3083 3885
Size: 416 53 83 399 24 1512 154 802
Cuts at: 3885 4843 442
Size: 958 1753
Fragments arranged by size:
1753 1512 958 802 416 399 154 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3056 1056
Size: 2000 4154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2977 4814 1239
Size: 879 859 1837 2579
Fragments arranged by size:
2579 1837 879 859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3245 4817 882
Size: 617 1746 1572 2219
Fragments arranged by size:
2219 1746 1572 617
BsaAI yAC'GTr
Cuts at: 493 1204 2656 3945 493
Size: 711 1452 1289 2702
Fragments arranged by size:
2702 1452 1289 711
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3961 4343 4978 275
Size: 53 83 186 936 2428 382 635 1451
Fragments arranged by size:
2428 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4587 4985 5259 5406 5817 4587
Size: 398 274 147 411 4924
Fragments arranged by size:
4924 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5929
Fragments arranged by size:
5929 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2115 2390 3022 3368 1186
Size: 929 275 632 346 3972
Fragments arranged by size:
3972 929 632 346 275
BseYI C'CCAG_C
Cuts at: 2349 3361 5357 5853 2349
Size: 1012 1996 496 2650
Fragments arranged by size:
2650 1996 1012 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 1163
Size: 573 1059 4522
Fragments arranged by size:
4522 1059 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2655 4215 4300 5371 2012
Size: 643 1560 85 1071 2795
Fragments arranged by size:
2795 1560 1071 643 85
BsmI GAATG_Cn'
Cuts at: 2382 3665 3758 2382
Size: 1283 93 4778
Fragments arranged by size:
4778 1283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2111 2655 3473 4215 4300 5371
Size: 399 222 99 544 818 742 85 1071
Cuts at: 5371 1391
Size: 2174
Fragments arranged by size:
2174 1071 818 742 544 399 222 99
85
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 2865 3136 4043 5773 2865
Size: 271 907 1730 3246
Fragments arranged by size:
3246 1730 907 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3509 844
Size: 2665 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 4041 1394
Size: 429 622 1596 3507
Fragments arranged by size:
3507 1596 622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2388 2511 4649 4823 65
Size: 1183 1140 123 2138 174 1396
Fragments arranged by size:
2138 1396 1183 1140 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 4798 4985 1700
Size: 110 2988 187 2869
Fragments arranged by size:
2988 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2854 96
Size: 2758 3396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6154
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 3269 2593
Size: 676 5478
BstYI r'GATC_y
Cuts at: 1972 2248 2686 3484 3851 4236 4253 5705 5791
Size: 276 438 798 367 385 17 1452 86
Cuts at: 5791 5803 6149 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 798 438 385 367 346 276
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5602
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 2971 3670 4464 4484 2971
Size: 699 794 20 4641
Fragments arranged by size:
4641 794 699 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 2232 3458 2232
Size: 1226 4928
DraIII CAC_nnn'GTG
Cuts at: 2659 2659
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 3601 4490
Size: 54 1425 50 275 18 183 1588 889
Cuts at: 4490 6119 8
Size: 1629 43
Fragments arranged by size:
1629 1588 1425 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4084 1353
Size: 2731 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2504 2942 2977 2984 4217 4814
Size: 393 486 386 438 35 7 1233 597
Cuts at: 4814 5601 5869 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1233 787 597 486 438 393 386
268 35 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2100 2486 2906 2100
Size: 386 420 5348
Fragments arranged by size:
5348 420 386
EcoO109I rG'GnC_Cy
Cuts at: 2072 2072
Size: 6154
EcoRI G'AATT_C
Cuts at: 2869 3463 3841 2869
Size: 594 378 5182
Fragments arranged by size:
5182 594 378
EcoRV GAT'ATC
Cuts at: 2410 2410
Size: 6154
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2962 5301 1678
Size: 1284 2339 2531
Fragments arranged by size:
2531 2339 1284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 4795 4827 4869
Size: 32 135 32 689 32 2502 32 42
Cuts at: 4869 4901 1373
Size: 32 2626
Fragments arranged by size:
2626 2502 689 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3498 3744 669
Size: 988 281 1560 246 3079
Fragments arranged by size:
3079 1560 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5268 5452 1305
Size: 3963 184 2007
Fragments arranged by size:
3963 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4332
Fragments arranged by size:
4332 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4112 4471 4630 518
Size: 648 2946 359 159 2042
Fragments arranged by size:
2946 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2137 3361 3570 4247 5395 5640 1606
Size: 459 72 1224 209 677 1148 245 2120
Fragments arranged by size:
2120 1224 1148 677 459 245 209 72
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5602
NdeI CA'TA_TG
Cuts at: 387 2947 3322 387
Size: 2560 375 3219
Fragments arranged by size:
3219 2560 375
NheI G'CTAG_C
Cuts at: 1052 3052 1052
Size: 2000 4154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 3512 5057 1132
Size: 97 2283 1545 2229
Fragments arranged by size:
2283 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2672 1348
Size: 1324 4830
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PfoI T'CCnGG_A
Cuts at: 2682 2682
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 2036 3724 2036
Size: 1688 4466
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2520 2552 2520
Size: 32 6122
PstI C_TGCA'G
Cuts at: 830 3506 830
Size: 2676 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2065 2137 3361 2065
Size: 72 1224 4858
Fragments arranged by size:
4858 1224 72
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 2939 4402 6114 2939
Size: 1463 1712 2979
Fragments arranged by size:
2979 1712 1463
SexAI A'CCwGG_T
Cuts at: 2206 2206
Size: 6154
SfcI C'TryA_G
Cuts at: 826 1045 2537 3435 3502 4637 5318 5509 826
Size: 219 1492 898 67 1135 681 191 1471
Fragments arranged by size:
1492 1471 1135 898 681 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3481 1872
Size: 1609 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4258 5159 5421 820
Size: 197 926 2315 901 262 1553
Fragments arranged by size:
2315 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 3593 513
Size: 552 2528 3074
Fragments arranged by size:
3074 2528 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4181 4198 4351 4536 4981 325
Size: 1255 2601 17 153 185 445 1498
Fragments arranged by size:
2601 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2854 2937 2968
Size: 274 80 33 51 1443 877 83 31
Cuts at: 2968 4400 6112 96
Size: 1432 1712 138
Fragments arranged by size:
1712 1443 1432 877 274 138 83 80
51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6114 6146 6114
Size: 32 6122
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3479 1870
Size: 1609 4545
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI