Restriction map for
pFN21AB5837
(Circular) (Six-base) MAPSORT of: pFN21AB5837.seq Check: 4951 from: 1 to: 5425
pFN21AB5837 5425 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2399 2399
Size: 5425
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2153 2746 2153
Size: 593 4832
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 3488 4872 5140 1632
Size: 578 1278 1384 268 1917
Fragments arranged by size:
1917 1384 1278 578 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 2311 4154 2311
Size: 1843 3582
AleI CACnn'nnGTG
Cuts at: 1166 2462 2465 1166
Size: 1296 3 4126
Fragments arranged by size:
4126 1296 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5425
AlwNI CAG_nnn'CTG
Cuts at: 3168 4740 3168
Size: 1572 3853
ApaI G_GGCC'C
Cuts at: 2621 2621
Size: 5425
ApaLI G'TGCA_C
Cuts at: 2079 3482 4638 2079
Size: 1403 1156 2866
Fragments arranged by size:
2866 1403 1156
ApoI r'AATT_y
Cuts at: 1493 2195 2734 2960 3112 5221 1493
Size: 702 539 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 539 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2750 1870
Size: 73 183 624 4545
Fragments arranged by size:
4545 624 183 73
BamHI G'GATC_C
Cuts at: 2031 2755 3122 2031
Size: 724 367 4334
Fragments arranged by size:
4334 724 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2746 4201 618
Size: 325 217 627 366 593 1455 1842
Fragments arranged by size:
1842 1455 627 593 366 325 217
BanII G_rGCy'C
Cuts at: 1391 2276 2621 2744 1391
Size: 885 345 123 4072
Fragments arranged by size:
4072 885 345 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BbvCI CC'TCA_GC
Cuts at: 2282 2484 2550 2282
Size: 202 66 5157
Fragments arranged by size:
5157 202 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 4036 136
Size: 107 122 71 631 605 1042 1322 1525
Fragments arranged by size:
1525 1322 1042 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2621 3486 4642 1790
Size: 293 538 865 1156 2573
Fragments arranged by size:
2573 1156 865 538 293
BmgBI CAC'GTC
Cuts at: 1318 2185 2418 2432 1318
Size: 867 233 14 4311
Fragments arranged by size:
4311 867 233 14
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2667 4085 1239
Size: 1428 1418 2579
Fragments arranged by size:
2579 1428 1418
Bpu10I CC'TnA_GC
Cuts at: 2282 2484 2550 2282
Size: 202 66 5157
Fragments arranged by size:
5157 202 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 4088 882
Size: 617 1131 1458 2219
Fragments arranged by size:
2219 1458 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 2084 3216 493
Size: 711 880 1132 2702
Fragments arranged by size:
2702 1132 880 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2434 3858 4256 4530 4677 5088 2434
Size: 1424 398 274 147 411 2771
Fragments arranged by size:
2771 1424 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2056 2086 1178
Size: 30 165 30 653 30 4517
Fragments arranged by size:
4517 653 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2664 1186
Size: 1478 3947
BseYI C'CCAG_C
Cuts at: 2269 2504 2602 2607 4628 5124 2269
Size: 235 98 5 2021 496 2570
Fragments arranged by size:
2570 2021 496 235 98 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 3636 4664 1540
Size: 275 186 874 761 1028 2301
Fragments arranged by size:
2301 1028 874 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 3486 3571 4642 2083
Size: 1403 85 1071 2866
Fragments arranged by size:
2866 1403 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2276 2621 2744 3486 3571 4642
Size: 399 293 193 345 123 742 85 1071
Cuts at: 4642 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 345 293 193 123
85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2399 2780 844
Size: 1555 381 3489
Fragments arranged by size:
3489 1555 381
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3920 4094 65
Size: 1183 2672 174 1396
Fragments arranged by size:
2672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2257 2623 4069 4256 1700
Size: 110 204 243 366 1446 187 2869
Fragments arranged by size:
2869 1446 366 243 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 2633 3485 4497 2633
Size: 852 1012 3561
Fragments arranged by size:
3561 1012 852
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 2464 2464
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2031 2755 3122 3507 3524 4976 5062 5074
Size: 59 724 367 385 17 1452 86 12
Cuts at: 5074 5420 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 724 385 367 346 86 59
17 12
Bsu36I CC'TnA_GG
Cuts at: 2229 2229
Size: 5425
BtgI C'CryG_G
Cuts at: 513 1065 2027 2107 2613 513
Size: 552 962 80 506 3325
Fragments arranged by size:
3325 962 552 506 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DraIII CAC_nnn'GTG
Cuts at: 2087 2087
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 2872 3761
Size: 54 1425 50 275 18 504 538 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 538 504 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 3355 1353
Size: 773 1229 3423
Fragments arranged by size:
3423 1229 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4012 4386 4532 387
Size: 1064 138 2423 374 146 1280
Fragments arranged by size:
2423 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2667 3488 4085 4872 5140 1239
Size: 393 578 457 821 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 578 457 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2553 2553
Size: 5425
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2282 4572 1678
Size: 604 2290 2531
Fragments arranged by size:
2531 2290 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2297 2329 4066
Size: 32 135 32 531 32 162 32 1737
Cuts at: 4066 4098 4140 4172 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1737 531 162 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2157 2750 2157
Size: 593 4832
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2462 2465 3383 3742 3901 518
Size: 648 1188 108 3 918 359 159 2042
Fragments arranged by size:
2042 1188 918 648 359 159 108 3
MspA1I CmG'CkG
Cuts at: 1606 2029 2269 2841 3518 4666 4911 1606
Size: 423 240 572 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 572 423 245 240
NcoI C'CATG_G
Cuts at: 513 1065 2613 513
Size: 552 1548 3325
Fragments arranged by size:
3325 1548 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2474 2783 4328 1132
Size: 97 1245 309 1545 2229
Fragments arranged by size:
2229 1545 1245 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2614 1108
Size: 1506 3919
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PmlI CAC'GTG
Cuts at: 2084 2084
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2617 2617
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2777 830
Size: 1947 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
PvuII CAG'CTG
Cuts at: 2269 2269
Size: 5425
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2773 3908 4589 4780 826
Size: 219 1728 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2474 2783 1132
Size: 1342 309 3774
Fragments arranged by size:
3774 1342 309
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2331 2613 2864 513
Size: 552 1266 282 251 3074
Fragments arranged by size:
3074 1266 552 282 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 2430 1316
Size: 1114 4311
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI