Restriction map for
pFN21AB5835
(Circular) (Six-base) MAPSORT of: pFN21AB5835.seq Check: 5026 from: 1 to: 6295
pFN21AB5835 6295 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 3549 4358 5742 6010 1632
Size: 983 934 809 1384 268 1917
Fragments arranged by size:
1917 1384 983 934 809 268
AflII C'TTAA_G
Cuts at: 820 1017 3272 820
Size: 197 2255 3843
Fragments arranged by size:
3843 2255 197
AflIII A'CryG_T
Cuts at: 1203 2389 5194 6171 1203
Size: 1186 2805 977 1327
Fragments arranged by size:
2805 1327 1186 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 2569 5024 2569
Size: 2455 3840
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 4038 5610 4038
Size: 1572 4723
ApaI G_GGCC'C
Cuts at: 2037 2177 3122 2037
Size: 140 945 5210
Fragments arranged by size:
5210 945 140
ApaLI G'TGCA_C
Cuts at: 2084 4352 5508 2084
Size: 2268 1156 2871
Fragments arranged by size:
2871 2268 1156
ApoI r'AATT_y
Cuts at: 1493 3138 3604 3830 3982 6091 1493
Size: 1645 466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1645 466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3465 3620 1870
Size: 73 1522 155 4545
Fragments arranged by size:
4545 1522 155 73
BamHI G'GATC_C
Cuts at: 3580 3625 3992 3580
Size: 45 367 5883
Fragments arranged by size:
5883 367 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3616 5071 618
Size: 325 217 627 1829 1455 1842
Fragments arranged by size:
1842 1829 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2177 2364 2958 3122 3315 3418 3466
Size: 646 140 187 594 164 193 103 48
Cuts at: 3466 3614 1391
Size: 148 4072
Fragments arranged by size:
4072 646 594 193 187 164 148 140
103 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2790 2824 4486 4520 1903
Size: 34 50 34 769 34 1662 34 3678
Fragments arranged by size:
3678 1662 769 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2578 4187 5397 2578
Size: 1609 1210 3476
Fragments arranged by size:
3476 1609 1210
BclI T'GATC_A
Cuts at: 1548 2931 3162 1548
Size: 1383 231 4681
Fragments arranged by size:
4681 1383 231
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3385 4906 136
Size: 107 122 71 631 605 1713 1521 1525
Fragments arranged by size:
1713 1525 1521 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2088 2177 3122 4356 5512 1790
Size: 247 51 89 945 1234 1156 2573
Fragments arranged by size:
2573 1234 1156 945 247 89 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2893 3031 3412
Size: 416 53 83 399 24 1476 138 381
Cuts at: 3412 3450 4026 4984 442
Size: 38 576 958 1753
Fragments arranged by size:
1753 1476 958 576 416 399 381 138
83 53 38 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3432 3432
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 2447 3053 3323 3329 4955 1239
Size: 1109 99 606 270 6 1626 2579
Fragments arranged by size:
2579 1626 1109 606 270 99 6
Bpu10I CC'TnA_GC
Cuts at: 3359 3359
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2866 3571 4420 5285 5583 2866
Size: 705 849 865 298 3578
Fragments arranged by size:
3578 865 849 705 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 4086 493
Size: 711 1356 1526 2702
Fragments arranged by size:
2702 1526 1356 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 1178
Size: 30 165 30 1068 30 4972
Fragments arranged by size:
4972 1068 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6295
BseYI C'CCAG_C
Cuts at: 2177 3227 5498 5994 2177
Size: 1050 2271 496 2478
Fragments arranged by size:
2478 2271 1050 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 1163
Size: 573 365 5357
Fragments arranged by size:
5357 573 365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2559 3418 3466 4356 4441 5512 2088
Size: 471 859 48 890 85 1071 2871
Fragments arranged by size:
2871 1071 890 859 471 85 48
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
BsmBI CGTCTCn'nnnn_
Cuts at: 2577 2577
Size: 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2088 2177 2364 2559 2958 3122
Size: 399 247 51 89 187 195 399 164
Cuts at: 3122 3315 3418 3466 3614 4356 4441 5512 1391
Size: 193 103 48 148 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 247 195 193
187 164 148 103 89 85 51 48
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2285 3650 844
Size: 1441 1365 3489
Fragments arranged by size:
3489 1441 1365
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 2599 4790 4964 65
Size: 1183 840 511 2191 174 1396
Fragments arranged by size:
2191 1396 1183 840 511 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 3580 3625 3992 4377 4394 5846 5932 5944
Size: 1608 45 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1608 1452 385 367 346 86 45
17 12
Bsu36I CC'TnA_GG
Cuts at: 3249 3381 3249
Size: 132 6163
BtgI C'CryG_G
Cuts at: 513 1065 2069 513
Size: 552 1004 4739
Fragments arranged by size:
4739 1004 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 3185 3811 4605 4625 3185
Size: 626 794 20 4855
Fragments arranged by size:
4855 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 2943 3599 2943
Size: 656 5639
DraIII CAC_nnn'GTG
Cuts at: 2330 2330
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3204 3742 4631
Size: 54 1425 50 275 18 1374 538 889
Cuts at: 4631 6260 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1374 889 538 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4225 1353
Size: 2872 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3416 3464 3416
Size: 48 6247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2447 2615 3053 3323 3329 3549
Size: 393 716 99 168 438 270 6 220
Cuts at: 3549 4358 4955 5742 6010 1239
Size: 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 716 597 438 393 270
268 220 168 99 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 3501 2129
Size: 1372 4923
EcoO109I rG'GnC_Cy
Cuts at: 2033 2034 2174 3253 2033
Size: 1 140 1079 5075
Fragments arranged by size:
5075 1079 140 1
EcoRI G'AATT_C
Cuts at: 3138 3604 3982 3138
Size: 466 378 5451
Fragments arranged by size:
5451 466 378
EcoRV GAT'ATC
Cuts at: 2650 2650
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2555 2587 2859 2891 3412
Size: 32 135 32 983 32 272 32 521
Cuts at: 3412 3444 4936 4968 5010 5042 1373
Size: 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 983 521 272 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 5409 5593 1305
Size: 1152 2952 184 2007
Fragments arranged by size:
2952 2007 1152 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3206 10
Size: 54 1425 343 1374 3099
Fragments arranged by size:
3099 1425 1374 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 3054 4253 4612 4771 518
Size: 648 1525 363 1199 359 159 2042
Fragments arranged by size:
2042 1525 1199 648 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 2834 3016 3711 4388 5536 5781 1606
Size: 1228 182 695 677 1148 245 2120
Fragments arranged by size:
2120 1228 1148 695 677 245 182
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5743
NdeI CA'TA_TG
Cuts at: 387 2974 387
Size: 2587 3708
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2188 2299 2393 3292 3335 3653 5198
Size: 97 959 111 94 899 43 318 1545
Cuts at: 5198 1132
Size: 2229
Fragments arranged by size:
2229 1545 959 899 318 111 97 94
43
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 2389 5194 2389
Size: 2805 3490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PmlI CAC'GTG
Cuts at: 2560 2560
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 4364 4396 5877 5909 2135
Size: 32 2197 32 1481 32 2521
Fragments arranged by size:
2521 2197 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3253 3253
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 3497 1613
Size: 1884 4411
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2033 2173 3118 2033
Size: 140 945 5210
Fragments arranged by size:
5210 945 140
PspXI vC'TCGA_Gb
Cuts at: 3465 3465
Size: 6295
PstI C_TGCA'G
Cuts at: 830 2142 2165 3647 830
Size: 1312 23 1482 3478
Fragments arranged by size:
3478 1482 1312 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2834 3016 2834
Size: 182 6113
SacI G_AGCT'C
Cuts at: 3418 3466 3418
Size: 48 6247
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2138 2161 2637 3643 4778 5459 5650
Size: 219 1093 23 476 1006 1135 681 191
Cuts at: 5650 826
Size: 1471
Fragments arranged by size:
1471 1135 1093 1006 681 476 219 191
23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2845 3272 3465 3586 4399 5300
Size: 197 926 902 427 193 121 813 901
Cuts at: 5300 5562 820
Size: 262 1553
Fragments arranged by size:
1553 926 902 901 813 427 262 197
193 121
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3292 3653 1132
Size: 2160 361 3774
Fragments arranged by size:
3774 2160 361
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 3102 3734 513
Size: 552 2037 632 3074
Fragments arranged by size:
3074 2037 632 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3411 4322 4339 4492 4677 5122 325
Size: 1255 1831 911 17 153 185 445 1498
Fragments arranged by size:
1831 1498 1255 911 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3348 4541 6253
Size: 274 80 33 51 1443 1371 1193 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1712 1443 1371 1193 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2448 2480 6255 6287 2448
Size: 32 3775 32 2456
Fragments arranged by size:
3775 2456 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 3465 1943
Size: 1522 4773
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 2147 4424 2147
Size: 2277 4018
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BsiWI BstXI BstZ17I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI