Restriction map for
pFN21AB5830
(Circular) (Six-base) MAPSORT of: pFN21AB5830.seq Check: 4041 from: 1 to: 6148
pFN21AB5830 6148 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3306 3491 1937
Size: 883 486 185 4594
Fragments arranged by size:
4594 883 486 185
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2669 4211 5595 5863 1632
Size: 479 558 1542 1384 268 1917
Fragments arranged by size:
1917 1542 1384 558 479 268
AflII C'TTAA_G
Cuts at: 820 1017 3158 820
Size: 197 2141 3810
Fragments arranged by size:
3810 2141 197
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 2046 1166
Size: 880 5268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 6116
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaI G_GGCC'C
Cuts at: 2347 2761 3195 2347
Size: 414 434 5300
Fragments arranged by size:
5300 434 414
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3329 3457 3683 3835 5944 1493
Size: 1836 128 226 152 2109 1697
Fragments arranged by size:
2109 1836 1697 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2730 3128 3473 1870
Size: 73 186 601 398 345 4545
Fragments arranged by size:
4545 601 398 345 186 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2628 2956 3469 4924 618
Size: 325 217 627 841 328 513 1455 1842
Fragments arranged by size:
1842 1455 841 627 513 328 325 217
BanII G_rGCy'C
Cuts at: 1391 2347 2761 3087 3195 3467 1391
Size: 956 414 326 108 272 4072
Fragments arranged by size:
4072 956 414 326 272 108
BbeI G_GCGC'C
Cuts at: 2960 2960
Size: 6148
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 2734 928
Size: 971 302 533 4342
Fragments arranged by size:
4342 971 533 302
BbvCI CC'TCA_GC
Cuts at: 3233 3233
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 2140 1548
Size: 592 5556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 3122 3565 3122
Size: 443 5705
Bme1580I G_kGCm'C
Cuts at: 1790 2347 2761 3195 4209 5365 1790
Size: 557 414 434 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 557 434 414
BmgBI CAC'GTC
Cuts at: 1318 2815 3253 3297 1318
Size: 1497 438 44 4169
Fragments arranged by size:
4169 1497 438 44
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 3334 3412
Size: 416 53 83 399 24 962 955 78
Cuts at: 3412 3879 4837 442
Size: 467 958 1753
Fragments arranged by size:
1753 962 958 955 467 416 399 83
78 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2265 2600 2990 3410 4808 1239
Size: 850 176 335 390 420 1398 2579
Fragments arranged by size:
2579 1398 850 420 390 335 176
Bpu10I CC'TnA_GC
Cuts at: 3233 3233
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2813 4273 5138 5436 2318
Size: 495 1460 865 298 3030
Fragments arranged by size:
3030 1460 865 495 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2272 3939 493
Size: 711 1068 1667 2702
Fragments arranged by size:
2702 1667 1068 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2957 3955 4337 4972
Size: 53 83 186 936 1424 998 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1451 1424 998 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 3209 3239 1178
Size: 30 165 30 750 30 1026 30 4087
Fragments arranged by size:
4087 1026 750 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 2383 2420 2765 1186
Size: 1123 74 37 345 4569
Fragments arranged by size:
4569 1123 345 74 37
BseYI C'CCAG_C
Cuts at: 3010 3149 3194 5351 5847 3010
Size: 139 45 2157 496 3311
Fragments arranged by size:
3311 2157 496 139 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2793 1163
Size: 573 1057 4518
Fragments arranged by size:
4518 1057 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3043 3598 4359 5387 1540
Size: 275 186 673 369 555 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 555 369 275 186
BsiHKAI G_wGCw'C
Cuts at: 3087 3222 4209 4294 5365 3087
Size: 135 987 85 1071 3870
Fragments arranged by size:
3870 1071 987 135 85
BsmI GAATG_Cn'
Cuts at: 2186 3659 3752 2186
Size: 1473 93 4582
Fragments arranged by size:
4582 1473 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 2761 3087 3195 3222 3467 4209
Size: 399 557 414 326 108 27 245 742
Cuts at: 4209 4294 5365 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 414 399 326 245
108 85 27
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3503 844
Size: 2659 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2755 4035 1394
Size: 429 932 1280 3507
Fragments arranged by size:
3507 1280 932 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2568 4643 4817 65
Size: 1183 1320 2075 174 1396
Fragments arranged by size:
2075 1396 1320 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 3068 4208 5220 3068
Size: 1140 1012 3996
Fragments arranged by size:
3996 1140 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstXI CCAn_nnnn'nTGG
Cuts at: 2048 2317 2048
Size: 269 5879
BstYI r'GATC_y
Cuts at: 1972 2547 3102 3478 3845 4230 4247 5699 5785
Size: 575 555 376 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 575 555 385 376 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2821 2821
Size: 6148
Bsu36I CC'TnA_GG
Cuts at: 2961 3288 2961
Size: 327 5821
BtgI C'CryG_G
Cuts at: 513 1065 2075 2361 2994 513
Size: 552 1010 286 633 3667
Fragments arranged by size:
3667 1010 633 552 286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3213 487
Size: 683 56 1987 3422
Fragments arranged by size:
3422 1987 683 56
BtsI GCAGTG_nn'
Cuts at: 2246 2640 3664 4458 4478 2246
Size: 394 1024 794 20 3916
Fragments arranged by size:
3916 1024 794 394 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 2863 2986
Size: 54 1425 50 275 18 697 336 123
Cuts at: 2986 3040 3432 3595 4484 6113 8
Size: 54 392 163 889 1629 43
Fragments arranged by size:
1629 1425 889 697 392 336 275 163
123 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 3595 1537
Size: 275 1228 555 4090
Fragments arranged by size:
4090 1228 555 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 3083 3093 4078 1353
Size: 734 996 10 985 3423
Fragments arranged by size:
3423 996 985 734 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3085 3085
Size: 6148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2111 2265 2600 2669 2990 3410
Size: 393 457 22 154 335 69 321 420
Cuts at: 3410 4211 4808 5595 5863 1239
Size: 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 457 420 393 335
321 268 154 69 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2480 2555 2480
Size: 75 6073
EcoO109I rG'GnC_Cy
Cuts at: 2134 2262 2344 2356 2611 2940 3166 3191 2134
Size: 128 82 12 255 329 226 25 5091
Fragments arranged by size:
5091 329 255 226 128 82 25 12
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2674 2506
Size: 168 5980
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2960 3076 5295 1678
Size: 1282 116 2219 2531
Fragments arranged by size:
2531 2219 1282 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 4789 4821 4863
Size: 32 135 32 1550 32 1635 32 42
Cuts at: 4863 4895 1373
Size: 32 2626
Fragments arranged by size:
2626 1635 1550 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3307 3492 3738 669
Size: 988 281 1369 185 246 3079
Fragments arranged by size:
3079 1369 988 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KasI G'GCGC_C
Cuts at: 2956 2956
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5262 5446 1305
Size: 3957 184 2007
Fragments arranged by size:
3957 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2865 10
Size: 54 1425 343 1033 3293
Fragments arranged by size:
3293 1425 1033 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2046 2315 2507 3092 4106 4465 4624
Size: 648 880 269 192 585 1014 359 159
Cuts at: 4624 518
Size: 2042
Fragments arranged by size:
2042 1014 880 648 585 359 269 192
159
MspA1I CmG'CkG
Cuts at: 1606 2188 2341 3403 3564 4241 5389 5634 1606
Size: 582 153 1062 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 1062 677 582 245 161 153
NarI GG'CG_CC
Cuts at: 2957 2957
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 2361 513
Size: 552 1296 4300
Fragments arranged by size:
4300 1296 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3040 3595 3040
Size: 555 5593
NspI r_CATG'y
Cuts at: 1132 1229 3506 5051 1132
Size: 97 2277 1545 2229
Fragments arranged by size:
2277 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PmlI CAC'GTG
Cuts at: 2272 2272
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 4217 4249 5730 5762 3209
Size: 32 976 32 1481 32 3595
Fragments arranged by size:
3595 1481 976 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2262 2356 2611 2940 3166 2262
Size: 94 255 329 226 5244
Fragments arranged by size:
5244 329 255 226 94
PshAI GACnn'nnGTC
Cuts at: 1613 2249 1613
Size: 636 5512
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspOMI G'GGCC_C
Cuts at: 2343 2757 3191 2343
Size: 414 434 5300
Fragments arranged by size:
5300 434 414
PstI C_TGCA'G
Cuts at: 830 2241 3500 830
Size: 1411 1259 3478
Fragments arranged by size:
3478 1411 1259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2188 2188
Size: 6148
SacI G_AGCT'C
Cuts at: 3087 3087
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3305 3490 1936
Size: 1369 185 4594
Fragments arranged by size:
4594 1369 185
SanDI GG'GwC_CC
Cuts at: 2940 2940
Size: 6148
SapI GCTCTTCn'nnn_
Cuts at: 2087 2087
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 2798 4396 6108 2798
Size: 1598 1712 2838
Fragments arranged by size:
2838 1712 1598
SfcI C'TryA_G
Cuts at: 826 1045 2237 3496 4631 5312 5503 826
Size: 219 1192 1259 1135 681 191 1471
Fragments arranged by size:
1471 1259 1192 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2958 2958
Size: 6148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 2732 3475 1872
Size: 860 743 4545
Fragments arranged by size:
4545 860 743
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2333 2792 3158 4252 5153 5415
Size: 197 926 390 459 366 1094 901 262
Cuts at: 5415 820
Size: 1553
Fragments arranged by size:
1553 1094 926 901 459 390 366 262
197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2361 2476 2574 3204 3587 513
Size: 552 1296 115 98 630 383 3074
Fragments arranged by size:
3074 1296 630 552 383 115 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 3002 4175 4192 4345 4530 4975
Size: 1255 633 789 1173 17 153 185 445
Cuts at: 4975 325
Size: 1498
Fragments arranged by size:
1498 1255 1173 789 633 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 4394 6106
Size: 274 80 33 51 1443 819 1598 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1712 1598 1443 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 2357 2389 6108 6140 2067
Size: 32 258 32 3719 32 2075
Fragments arranged by size:
3719 2075 258 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3254 1316
Size: 1938 4210
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3211 3211
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 2730 3473 1870
Size: 860 743 4545
Fragments arranged by size:
4545 860 743
XmnI GAAnn'nnTTC
Cuts at: 2456 2930 4277 2456
Size: 474 1347 4327
Fragments arranged by size:
4327 1347 474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI RsrII SacII SexAI SfiI
SrfI SwaI