Restriction map for
pFN21AB5826
(Circular) (Six-base) MAPSORT of: pFN21AB5826.seq Check: 6281 from: 1 to: 6130
pFN21AB5826 6130 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1995 2801 2858 3188 3325 4193 5577 5845
Size: 363 806 57 330 137 868 1384 268
Cuts at: 5845 1632
Size: 1917
Fragments arranged by size:
1917 1384 868 806 363 330 268 137
57
AfeI AGC'GCT
Cuts at: 2174 2174
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2855 5029 6006 1203
Size: 1652 2174 977 1327
Fragments arranged by size:
2174 1652 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 2109 2154 2602 3873 5445 2109
Size: 45 448 1271 1572 2794
Fragments arranged by size:
2794 1572 1271 448 45
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2390 2423 2390
Size: 33 6097
BamHI G'GATC_C
Cuts at: 2878 3416 3460 3827 2878
Size: 538 44 367 5181
Fragments arranged by size:
5181 538 367 44
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2709 3002 3176 3208 3451
Size: 325 217 627 922 293 174 32 243
Cuts at: 3451 4906 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 922 627 325 293 243 217
174 32
BanII G_rGCy'C
Cuts at: 1391 2688 3449 1391
Size: 1297 761 4072
Fragments arranged by size:
4072 1297 761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5159
BbvCI CC'TCA_GC
Cuts at: 2849 2849
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BfrBI ATG'CAT
Cuts at: 2121 2121
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2657 4741 136
Size: 107 122 71 631 605 985 2084 1525
Fragments arranged by size:
2084 1525 985 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 2719 3547 2719
Size: 828 5302
Bme1580I G_kGCm'C
Cuts at: 1790 2264 2350 3007 3108 4191 5347 1790
Size: 474 86 657 101 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 657 474 101 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2344 3043 3861
Size: 416 53 83 399 24 927 699 818
Cuts at: 3861 4819 442
Size: 958 1753
Fragments arranged by size:
1753 958 927 818 699 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2213 1056
Size: 1157 4973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2624 2787 4790 1239
Size: 1166 219 163 2003 2579
Fragments arranged by size:
2579 2003 1166 219 163
Bpu10I CC'TnA_GC
Cuts at: 2849 2849
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2408 4255 5120 5418 2168
Size: 240 1847 865 298 2880
Fragments arranged by size:
2880 1847 865 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3262 4793 882
Size: 617 1763 1531 2219
Fragments arranged by size:
2219 1763 1531 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 2985 6124 2985
Size: 3139 2991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 1178
Size: 30 165 30 1866 30 4009
Fragments arranged by size:
4009 1866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2405 1186
Size: 868 351 4911
Fragments arranged by size:
4911 868 351
BseYI C'CCAG_C
Cuts at: 2337 2661 2946 3006 5333 5829 2337
Size: 324 285 60 2327 496 2638
Fragments arranged by size:
2638 2327 496 324 285 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2301 2382 2573 2880 1163
Size: 573 565 81 191 307 4413
Fragments arranged by size:
4413 573 565 307 191 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2688 4191 4276 5347 2688
Size: 1503 85 1071 3471
Fragments arranged by size:
3471 1503 1071 85
BsmI GAATG_Cn'
Cuts at: 2123 3641 3734 2123
Size: 1518 93 4519
Fragments arranged by size:
4519 1518 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2350 2688 3007 3108 3449 4191
Size: 399 474 86 338 319 101 341 742
Cuts at: 4191 4276 5347 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 341 338 319
101 86 85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 4774 4961 1700
Size: 110 356 2608 187 2869
Fragments arranged by size:
2869 2608 356 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3112 794
Size: 2318 3812
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2878 3416 3460 3827 4212 4229 5681 5767
Size: 906 538 44 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 906 538 385 367 346 86
44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2376 2897 3280 513
Size: 552 939 372 521 383 3363
Fragments arranged by size:
3363 939 552 521 383 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2289 3646 4440 4460 2289
Size: 1357 794 20 3959
Fragments arranged by size:
3959 1357 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3074 5137 809
Size: 507 1758 2063 1802
Fragments arranged by size:
2063 1802 1758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 3577 4466
Size: 54 1425 50 275 18 268 1479 889
Cuts at: 4466 6095 8
Size: 1629 43
Fragments arranged by size:
1629 1479 1425 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 4060 1353
Size: 1611 1096 3423
Fragments arranged by size:
3423 1611 1096
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2686
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2405 2624 2787 2801 2858 3188
Size: 393 363 410 219 163 14 57 330
Cuts at: 3188 3325 4193 4790 5577 5845 1239
Size: 137 868 597 787 268 1524
Fragments arranged by size:
1524 868 787 597 410 393 363 330
268 219 163 137 57 14
EcoO109I rG'GnC_Cy
Cuts at: 2329 2674 2785 3067 3323 2329
Size: 345 111 282 256 5136
Fragments arranged by size:
5136 345 282 256 111
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2951 3124 3148 2951
Size: 173 24 5933
Fragments arranged by size:
5933 173 24
FspI TGC'GCA
Cuts at: 3095 4636 3095
Size: 1541 4589
HaeII r_GCGC'y
Cuts at: 1678 2176 5277 1678
Size: 498 3101 2531
Fragments arranged by size:
3101 2531 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 3269 3301 4771
Size: 32 135 32 1211 32 454 32 1470
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1470 1211 454 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 5244 5428 1305
Size: 1146 2793 184 2007
Fragments arranged by size:
2793 2007 1146 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 4040
Fragments arranged by size:
4040 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4088 4447 4606 518
Size: 648 2922 359 159 2042
Fragments arranged by size:
2922 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2593 2626 3546 4223 5371 5616 1606
Size: 987 33 920 677 1148 245 2120
Fragments arranged by size:
2120 1148 987 920 677 245 33
NaeI GCC'GGC
Cuts at: 2168 2168
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 2004 2376 3280 513
Size: 552 939 372 904 3363
Fragments arranged by size:
3363 939 904 552 372
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2166 2166
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 2209 1052
Size: 1157 4973
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NsiI A_TGCA'T
Cuts at: 2123 2123
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2052 2859 3488 5033 1132
Size: 97 823 807 629 1545 2229
Fragments arranged by size:
2229 1545 823 807 629 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2855 5029 2855
Size: 2174 3956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2898 1108
Size: 1269 521 4340
Fragments arranged by size:
4340 1269 521
PfoI T'CCnGG_A
Cuts at: 2754 2771 3234 2754
Size: 17 463 5650
Fragments arranged by size:
5650 463 17
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 3067 3323 2785
Size: 282 256 5592
Fragments arranged by size:
5592 282 256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2598 2739 3083 3482 830
Size: 1768 141 344 399 3478
Fragments arranged by size:
3478 1768 399 344 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2593 2626 2593
Size: 33 6097
SacI G_AGCT'C
Cuts at: 2688 2688
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2594 2678 2735 3079 3478 4613 5294
Size: 219 1549 84 57 344 399 1135 681
Cuts at: 5294 5485 826
Size: 191 1471
Fragments arranged by size:
1549 1471 1135 681 399 344 219 191
84 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2657 2657
Size: 6130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2387 4234 5135 5397 820
Size: 197 926 240 204 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 240 204 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 3403 4054 5
Size: 47 3351 651 2081
Fragments arranged by size:
3351 2081 651 47
StuI AGG'CCT
Cuts at: 1517 3062 1517
Size: 1545 4585
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 3280 3569 513
Size: 552 939 372 904 289 3074
Fragments arranged by size:
3074 939 904 552 372 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2960 2990 3062 4157 4174 4327 4512
Size: 1255 1380 30 72 1095 17 153 185
Cuts at: 4512 4957 325
Size: 445 1498
Fragments arranged by size:
1498 1380 1255 1095 445 185 153 72
30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4376 6088 96
Size: 274 80 33 51 1443 2399 1712 138
Fragments arranged by size:
2399 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2350 2382 2863 2878 2895 2910 6090 6122 2350
Size: 32 481 15 17 15 3180 32 2358
Fragments arranged by size:
3180 2358 481 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NarI NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI
XcmI