Restriction map for
pFN21AB5824
(Circular) (Six-base) MAPSORT of: pFN21AB5824.seq Check: 8402 from: 1 to: 6133
pFN21AB5824 6133 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2142 3476 1937
Size: 205 1334 4594
Fragments arranged by size:
4594 1334 205
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 2561 2826 3392 4196 5580 5848 1632
Size: 486 443 265 566 804 1384 268 1917
Fragments arranged by size:
1917 1384 804 566 486 443 268 265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 3047 5032 6009 1203
Size: 1844 1985 977 1327
Fragments arranged by size:
1985 1844 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2210 2552 3289 3442 3668 3820 5929 1493
Size: 717 342 737 153 226 152 2109 1697
Fragments arranged by size:
2109 1697 737 717 342 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3458 1870
Size: 73 889 626 4545
Fragments arranged by size:
4545 889 626 73
BamHI G'GATC_C
Cuts at: 3095 3463 3830 3095
Size: 368 367 5398
Fragments arranged by size:
5398 368 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 3086 3454 4909 618
Size: 325 217 627 270 1029 368 1455 1842
Fragments arranged by size:
1842 1455 1029 627 368 325 270 217
BanII G_rGCy'C
Cuts at: 1391 3452 1391
Size: 2061 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 928
Size: 971 132 5030
Fragments arranged by size:
5030 971 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2983 4025 5235 2983
Size: 1042 1210 3881
Fragments arranged by size:
3881 1210 1042
BclI T'GATC_A
Cuts at: 1548 3055 1548
Size: 1507 4626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2169 6128 2169
Size: 3959 2174
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2016 4194 5350 1790
Size: 226 2178 1156 2573
Fragments arranged by size:
2573 2178 1156 226
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 2631 2859
Size: 416 53 83 399 24 621 593 228
Cuts at: 2859 3864 4822 442
Size: 1005 958 1753
Fragments arranged by size:
1753 1005 958 621 593 416 399 228
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2514 2634 2892 2932 4793 1239
Size: 1037 238 120 258 40 1861 2579
Fragments arranged by size:
2579 1861 1037 258 238 120 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2795 4258 5123 5421 2795
Size: 1463 865 298 3507
Fragments arranged by size:
3507 1463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 2174 6127 2174
Size: 3953 2180
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4566 4964 5238 5385 5796 4566
Size: 398 274 147 411 4903
Fragments arranged by size:
4903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2286 2706 1186
Size: 1100 420 4613
Fragments arranged by size:
4613 1100 420
BseYI C'CCAG_C
Cuts at: 3039 5336 5832 3039
Size: 2297 496 3340
Fragments arranged by size:
3340 2297 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4194 4279 5350 4194
Size: 85 1071 4977
Fragments arranged by size:
4977 1071 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 3452 4194 4279 5350 1391
Size: 399 226 1436 742 85 1071 2174
Fragments arranged by size:
2174 1436 1071 742 399 226 85
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 2194 4022 5752 2194
Size: 1828 1730 2575
Fragments arranged by size:
2575 1828 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3488 844
Size: 2644 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 4628 4802 65
Size: 1183 1362 2018 174 1396
Fragments arranged by size:
2018 1396 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2169 3095 3463 3830 4215 4232 5684 5770
Size: 197 926 368 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 926 385 368 367 346 197
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2624 513
Size: 552 939 620 4022
Fragments arranged by size:
4022 939 620 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2118 3649 4443 4463 2016
Size: 102 1531 794 20 3686
Fragments arranged by size:
3686 1531 794 102 20
ClaI AT'CG_AT
Cuts at: 1554 2706 1554
Size: 1152 4981
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2488 2523 529
Size: 35 1924 35 4139
Fragments arranged by size:
4139 1924 35 35
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3580 4469 6098
Size: 54 1425 50 275 18 1750 889 1629
Cuts at: 6098 8
Size: 43
Fragments arranged by size:
1750 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 2542 4063 1353
Size: 1184 5 1521 3423
Fragments arranged by size:
3423 1521 1184 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2276 2514 2561 2634 2826 2892
Size: 393 486 158 238 47 73 192 66
Cuts at: 2892 2932 3392 4196 4793 5580 5848 1239
Size: 40 460 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 486 460 393 268
238 192 158 73 66 47 40
EcoO109I rG'GnC_Cy
Cuts at: 2829 2829
Size: 6133
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 3382 2093
Size: 1289 4844
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 2738 2770 4774
Size: 32 135 32 992 32 142 32 2004
Cuts at: 4774 4806 4848 4880 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2004 992 142 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3322 3477 3723 669
Size: 988 281 1384 155 246 3079
Fragments arranged by size:
3079 1384 988 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 5247 5431 1305
Size: 1092 2850 184 2007
Fragments arranged by size:
2850 2007 1092 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4311
Fragments arranged by size:
4311 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2199 2306 4091 4450 4609 518
Size: 648 843 190 107 1785 359 159 2042
Fragments arranged by size:
2042 1785 843 648 359 190 159 107
MspA1I CmG'CkG
Cuts at: 1606 2646 3118 3549 4226 5374 5619 1606
Size: 1040 472 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 472 431 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2624 513
Size: 552 939 620 4022
Fragments arranged by size:
4022 939 620 552
NdeI CA'TA_TG
Cuts at: 387 2521 3060 387
Size: 2134 539 3460
Fragments arranged by size:
3460 2134 539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2311 3051 3491 5036 1132
Size: 97 1082 740 440 1545 2229
Fragments arranged by size:
2229 1545 1082 740 440 97
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 5434
PciI A'CATG_T
Cuts at: 3047 5032 3047
Size: 1985 4148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2646 1613
Size: 1033 5100
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2646 2646
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 2261 2595 2945 4381 6093 2261
Size: 334 350 1436 1712 2301
Fragments arranged by size:
2301 1712 1436 350 334
SfcI C'TryA_G
Cuts at: 826 1045 2263 3481 4616 5297 5488 826
Size: 219 1218 1218 1135 681 191 1471
Fragments arranged by size:
1471 1218 1218 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 4237 5138 5400 820
Size: 197 926 867 1427 901 262 1553
Fragments arranged by size:
1553 1427 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 2311 3491 1132
Size: 1179 1180 3774
Fragments arranged by size:
3774 1180 1179
SspI AAT'ATT
Cuts at: 5 52 2158 2246 3315 4057 5
Size: 47 2106 88 1069 742 2081
Fragments arranged by size:
2106 2081 1069 742 88 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2624 2693 3572 513
Size: 552 939 620 69 879 3074
Fragments arranged by size:
3074 939 879 620 552 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 2593 2943
Size: 274 80 33 51 1443 282 334 350
Cuts at: 2943 4379 6091 96
Size: 1436 1712 138
Fragments arranged by size:
1712 1443 1436 350 334 282 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 6093 6125 2873
Size: 32 3188 32 2881
Fragments arranged by size:
3188 2881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2574 2574
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 2409 4262 2409
Size: 1853 4280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI