Restriction map for
pFN21AB5819
(Circular) (Six-base) MAPSORT of: pFN21AB5819.seq Check: 7995 from: 1 to: 5941
pFN21AB5819 5941 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2788 3284 1937
Size: 851 496 4594
Fragments arranged by size:
4594 851 496
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2622 3011 4004 5388 5656 1632
Size: 990 389 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 990 389 268
AfeI AGC'GCT
Cuts at: 2918 2918
Size: 5941
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2412 2454 4840 5817 1203
Size: 1209 42 2386 977 1327
Fragments arranged by size:
2386 1327 1209 977 42
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2421 3251 1184
Size: 1237 830 3874
Fragments arranged by size:
3874 1237 830
AlwNI CAG_nnn'CTG
Cuts at: 2824 3196 3684 5256 2824
Size: 372 488 1572 3509
Fragments arranged by size:
3509 1572 488 372
ApaI G_GGCC'C
Cuts at: 2479 2479
Size: 5941
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2326 2332 2703 3250 3476 3628 5737 1493
Size: 833 6 371 547 226 152 2109 1697
Fragments arranged by size:
2109 1697 833 547 371 226 152 6
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 2873 3266 1870
Size: 73 545 385 393 4545
Fragments arranged by size:
4545 545 393 385 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3262 4717 618
Size: 325 217 627 1475 1455 1842
Fragments arranged by size:
1842 1475 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2479 2560 2782 3100 3260 1391
Size: 1088 81 222 318 160 4072
Fragments arranged by size:
4072 1088 318 222 160 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BbvCI CC'TCA_GC
Cuts at: 2658 2658
Size: 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2954 2988 4132 4166 1903
Size: 34 1017 34 1144 34 3678
Fragments arranged by size:
3678 1144 1017 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 4552 136
Size: 107 122 71 631 605 412 2468 1525
Fragments arranged by size:
2468 1525 631 605 412 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2479 2956 4002 5158 1790
Size: 689 477 1046 1156 2573
Fragments arranged by size:
2573 1156 1046 689 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2053 2757 3672
Size: 416 53 83 399 24 636 704 915
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 958 915 704 636 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2146 2635 2670 3092 4601 1239
Size: 891 16 489 35 422 1509 2579
Fragments arranged by size:
2579 1509 891 489 422 35 16
Bpu10I CC'TnA_GC
Cuts at: 2480 2658 3133 2480
Size: 178 475 5288
Fragments arranged by size:
5288 475 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3044 4066 4931 5229 3044
Size: 1022 865 298 3756
Fragments arranged by size:
3756 1022 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2106 2861 4604 882
Size: 617 607 755 1743 2219
Fragments arranged by size:
2219 1743 755 617 607
BsaAI yAC'GTr
Cuts at: 493 1204 2413 3732 493
Size: 711 1209 1319 2702
Fragments arranged by size:
2702 1319 1209 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2232 2249 2262 2279 1178
Size: 30 165 30 829 17 13 17 4840
Fragments arranged by size:
4840 829 165 30 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2891 3079 1186
Size: 1021 684 188 4048
Fragments arranged by size:
4048 1021 684 188
BseYI C'CCAG_C
Cuts at: 2060 5144 5640 2060
Size: 3084 496 2361
Fragments arranged by size:
3084 2361 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3221 1163
Size: 573 1485 3883
Fragments arranged by size:
3883 1485 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3100 4002 4087 5158 2313
Size: 787 902 85 1071 3096
Fragments arranged by size:
3096 1071 902 787 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2189
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2479 2560 2782 2956 3100 3260
Size: 399 523 166 81 222 174 144 160
Cuts at: 3260 4002 4087 5158 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 222 174 166
160 144 85 81
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 844
Size: 2452 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2881 3828 1394
Size: 429 1058 947 3507
Fragments arranged by size:
3507 1058 947 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2853 2958 4585 4772 1700
Size: 110 1043 105 1627 187 2869
Fragments arranged by size:
2869 1627 1043 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2312 4001 5013 2312
Size: 1689 1012 3240
Fragments arranged by size:
3240 1689 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 794
Size: 2135 3806
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3123 513
Size: 552 2058 3331
Fragments arranged by size:
3331 2058 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2102 487
Size: 683 56 876 4326
Fragments arranged by size:
4326 876 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 2424 2439 3457 4251 4271 2176
Size: 248 15 1018 794 20 3846
Fragments arranged by size:
3846 1018 794 248 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 2841 3106
Size: 54 1425 50 275 18 640 371 265
Cuts at: 3106 3388 4277 5906 8
Size: 282 889 1629 43
Fragments arranged by size:
1629 1425 889 640 371 282 275 265
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2184 2221 2843 3210 3871 1353
Size: 711 120 37 622 367 661 3423
Fragments arranged by size:
3423 711 661 622 367 120 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3098 3098
Size: 5941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2146 2622 2635 2670 3011 3092
Size: 393 498 16 476 13 35 341 81
Cuts at: 3092 4004 4601 5388 5656 1239
Size: 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 498 476 393 341
268 81 35 16 13
EcoO109I rG'GnC_Cy
Cuts at: 2476 2645 3112 2476
Size: 169 467 5305
Fragments arranged by size:
5305 467 169
EcoRI G'AATT_C
Cuts at: 2332 2703 3250 3628 2332
Size: 371 547 378 4645
Fragments arranged by size:
4645 547 378 371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2445 2920 5088 1678
Size: 767 475 2168 2531
Fragments arranged by size:
2531 2168 767 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2121 2144 2153 2176 2198
Size: 32 135 32 549 23 9 23 22
Cuts at: 2198 2230 2232 2264 2519 2551 4582 4614 4656
Size: 32 2 32 255 32 2031 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 549 255 135 42 32 32
32 32 32 32 32 23 23 22
9 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2789 3285 3531 669
Size: 988 281 851 496 246 3079
Fragments arranged by size:
3079 988 851 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1994 2164 3162 5055 5239 1305
Size: 689 170 998 1893 184 2007
Fragments arranged by size:
2007 1893 998 689 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3108 10
Size: 54 1425 343 1276 2843
Fragments arranged by size:
2843 1425 1276 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3899 4258 4417 518
Size: 648 2733 359 159 2042
Fragments arranged by size:
2733 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2821 2950 2968 3140 3160 3357 4034 5182
Size: 1215 129 18 172 20 197 677 1148
Cuts at: 5182 5427 1606
Size: 245 2120
Fragments arranged by size:
2120 1215 1148 677 245 197 172 129
20 18
NaeI GCC'GGC
Cuts at: 2855 2960 2855
Size: 105 5836
NcoI C'CATG_G
Cuts at: 513 1065 3123 513
Size: 552 2058 3331
Fragments arranged by size:
3331 2058 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2853 2958 2853
Size: 105 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2458 2932 3299 4844 1132
Size: 97 1229 474 367 1545 2229
Fragments arranged by size:
2229 1545 1229 474 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 2454 4840 2454
Size: 2386 3555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3196 1108
Size: 2088 3853
PfoI T'CCnGG_A
Cuts at: 2107 2107
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PmlI CAC'GTG
Cuts at: 2413 2413
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 4010 4042 5523 5555 3215
Size: 32 763 32 1481 32 3601
Fragments arranged by size:
3601 1481 763 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2645 3112 2645
Size: 467 5474
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2475 2475
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2431 2820 2967 3293 830
Size: 1601 389 147 326 3478
Fragments arranged by size:
3478 1601 389 326 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 3140 3160 3140
Size: 20 5921
SacI G_AGCT'C
Cuts at: 3100 3100
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 2787 3283 1936
Size: 851 496 4594
Fragments arranged by size:
4594 851 496
SanDI GG'GwC_CC
Cuts at: 2645 2645
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2064 2843 3210 2064
Size: 779 367 4795
Fragments arranged by size:
4795 779 367
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 2607 4189 5901 2607
Size: 1582 1712 2647
Fragments arranged by size:
2647 1712 1582
SfcI C'TryA_G
Cuts at: 826 1045 2427 2816 2963 3289 4424 5105 5296
Size: 219 1382 389 147 326 1135 681 191
Cuts at: 5296 826
Size: 1471
Fragments arranged by size:
1471 1382 1135 681 389 326 219 191
147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2490 3268 1872
Size: 618 778 4545
Fragments arranged by size:
4545 778 618
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3059 4045 4946 5208 820
Size: 197 926 1116 986 901 262 1553
Fragments arranged by size:
1553 1116 986 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 2932 3299 1132
Size: 1800 367 3774
Fragments arranged by size:
3774 1800 367
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5941
StyI C'CwwG_G
Cuts at: 513 1065 2116 3123 3380 513
Size: 552 1051 1007 257 3074
Fragments arranged by size:
3074 1051 1007 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2605 4187 5899
Size: 274 80 33 51 1443 628 1582 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1582 1443 628 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2488 3266 1870
Size: 618 778 4545
Fragments arranged by size:
4545 778 618
XmnI GAAnn'nnTTC
Cuts at: 3152 4070 3152
Size: 918 5023
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI XcmI