Restriction map for
pFN21AB5815
(Circular) (Six-base) MAPSORT of: pFN21AB5815.seq Check: 9736 from: 1 to: 6118
pFN21AB5815 6118 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4181 5565 5833 1632
Size: 2549 1384 268 1917
Fragments arranged by size:
2549 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 3305 1166
Size: 2139 3979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 2360 3337 3861 5433 2360
Size: 977 524 1572 3045
Fragments arranged by size:
3045 1572 977 524
ApaI G_GGCC'C
Cuts at: 2856 2856
Size: 6118
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2590 3427 3653 3805 5914 1493
Size: 1097 837 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 837 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 3443 1870
Size: 73 226 1274 4545
Fragments arranged by size:
4545 1274 226 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2413 2451 3268 3439 4894
Size: 325 217 627 626 38 817 171 1455
Cuts at: 4894 618
Size: 1842
Fragments arranged by size:
1842 1455 817 627 626 325 217 171
38
BanII G_rGCy'C
Cuts at: 1391 2856 2898 3089 3437 1391
Size: 1465 42 191 348 4072
Fragments arranged by size:
4072 1465 348 191 42
BbeI G_GCGC'C
Cuts at: 2455 2455
Size: 6118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3342 4729 136
Size: 107 122 71 631 605 1670 1387 1525
Fragments arranged by size:
1670 1525 1387 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3285 6113 3285
Size: 2828 3290
BlpI GC'TnA_GC
Cuts at: 3035 3535 3035
Size: 500 5618
Bme1580I G_kGCm'C
Cuts at: 1790 2418 2856 3273 4179 5335 1790
Size: 628 438 417 906 1156 2573
Fragments arranged by size:
2573 1156 906 628 438 417
BmgBI CAC'GTC
Cuts at: 1318 2284 1318
Size: 966 5152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2287 2552 3849
Size: 416 53 83 399 24 870 265 1297
Cuts at: 3849 4807 442
Size: 958 1753
Fragments arranged by size:
1753 1297 958 870 416 399 265 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2746 3319 2629
Size: 117 573 5428
Fragments arranged by size:
5428 573 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4778 1239
Size: 3539 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 4243 5108 5406 2843
Size: 1400 865 298 3555
Fragments arranged by size:
3555 1400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2461 2926 3909 493
Size: 711 1257 465 983 2702
Fragments arranged by size:
2702 1257 983 711 465
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2452 3053 3261 3925
Size: 53 83 186 936 919 601 208 664
Cuts at: 3925 4307 4942 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 919 664 635 601 382 208
186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2504 3167 3185 3194 1186
Size: 1045 273 663 18 9 4110
Fragments arranged by size:
4110 1045 663 273 18 9
BseYI C'CCAG_C
Cuts at: 2977 5321 5817 2977
Size: 2344 496 3278
Fragments arranged by size:
3278 2344 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2591 3116 3229 1163
Size: 573 855 525 113 4052
Fragments arranged by size:
4052 855 573 525 113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 3568 4329 5357 1540
Size: 275 186 139 1428 761 1028 2301
Fragments arranged by size:
2301 1428 1028 761 275 186 139
BsiHKAI G_wGCw'C
Cuts at: 2223 2307 3041 3089 4179 4264 5335 2223
Size: 84 734 48 1090 85 1071 3006
Fragments arranged by size:
3006 1090 1071 734 85 84 48
BsmI GAATG_Cn'
Cuts at: 3031 3629 3722 3031
Size: 598 93 5427
Fragments arranged by size:
5427 598 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2307 2418 2856 2898 3041 3089
Size: 399 433 84 111 438 42 143 48
Cuts at: 3089 3273 3437 4179 4264 5335 1391
Size: 184 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 438 433 399 184 164
143 111 85 84 48 42
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 3132 4007 5737 3132
Size: 875 1730 3513
Fragments arranged by size:
3513 1730 875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2056 2097 2613 4005 1394
Size: 429 206 27 41 516 1392 3507
Fragments arranged by size:
3507 1392 516 429 206 41 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 2952 4613 4787 65
Size: 1183 1704 1661 174 1396
Fragments arranged by size:
1704 1661 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 2139 2410 4762 4949 1700
Size: 110 260 69 271 2352 187 2869
Fragments arranged by size:
2869 2352 271 260 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 2249 4178 5190 2249
Size: 1929 1012 3177
Fragments arranged by size:
3177 1929 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstXI CCAn_nnnn'nTGG
Cuts at: 2549 3364 2549
Size: 815 5303
BstYI r'GATC_y
Cuts at: 1972 2679 3240 3285 3448 3815 4200 4217 5669
Size: 707 561 45 163 367 385 17 1452
Cuts at: 5669 5755 5767 6113 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 707 561 385 367 346 163
86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 3150 3150
Size: 6118
BtgI C'CryG_G
Cuts at: 513 1065 2418 2889 513
Size: 552 1353 471 3742
Fragments arranged by size:
3742 1353 552 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3634 4428 4448 2358
Size: 1276 794 20 4028
Fragments arranged by size:
4028 1276 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2936 5125 809
Size: 507 1620 2189 1802
Fragments arranged by size:
2189 1802 1620 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3565 4454
Size: 54 1425 50 275 18 1276 459 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1276 889 459 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 4048 1353
Size: 1017 1678 3423
Fragments arranged by size:
3423 1678 1017
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3087 3087
Size: 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4181 4778 5565 5833 1239
Size: 393 2549 597 787 268 1524
Fragments arranged by size:
2549 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2876 2876
Size: 6118
EcoO109I rG'GnC_Cy
Cuts at: 2545 2853 3121 3334 2545
Size: 308 268 213 5329
Fragments arranged by size:
5329 308 268 213
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2166 2211 2455 5265 1678
Size: 488 45 244 2810 2531
Fragments arranged by size:
2810 2531 488 244 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3354 3377 3386 3409 4759
Size: 32 135 32 1782 23 9 23 1350
Cuts at: 4759 4791 4833 4865 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1782 1350 135 42 32 32 32
32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 2451 2451
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5232 5416 1305
Size: 684 3243 184 2007
Fragments arranged by size:
3243 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3137 3305 4076 4435 4594 518
Size: 648 1149 822 168 771 359 159 2042
Fragments arranged by size:
2042 1149 822 771 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2977 3534 4211 5359 5604 1606
Size: 552 819 557 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 677 557 552 245
NarI GG'CG_CC
Cuts at: 2452 2452
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5566
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2719 2974 3476 5021 1132
Size: 97 1490 255 502 1545 2229
Fragments arranged by size:
2229 1545 1490 502 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2638 1108
Size: 1530 4588
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3121 3121
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 3119 1613
Size: 1506 4612
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2852 2852
Size: 6118
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2977 2977
Size: 6118
SacI G_AGCT'C
Cuts at: 3089 3089
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SapI GCTCTTCn'nnn_
Cuts at: 2370 2370
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SexAI A'CCwGG_T
Cuts at: 2823 2871 2823
Size: 48 6070
SfcI C'TryA_G
Cuts at: 826 1045 3466 4601 5282 5473 826
Size: 219 2421 1135 681 191 1471
Fragments arranged by size:
2421 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3342 3342
Size: 6118
SfoI GGC'GCC
Cuts at: 2453 2453
Size: 6118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 2410 1700
Size: 710 5408
SmaI CCC'GGG
Cuts at: 1872 2171 3445 1872
Size: 299 1274 4545
Fragments arranged by size:
4545 1274 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 4222 5123 5385 820
Size: 197 926 915 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 915 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 2974 3476 1132
Size: 1842 502 3774
Fragments arranged by size:
3774 1842 502
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2689 2864 1517
Size: 1172 175 4771
Fragments arranged by size:
4771 1172 175
StyI C'CwwG_G
Cuts at: 513 1065 2901 3557 513
Size: 552 1836 656 3074
Fragments arranged by size:
3074 1836 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4145 4162 4315 4500 4945 325
Size: 1255 2565 17 153 185 445 1498
Fragments arranged by size:
2565 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 4364 6076
Size: 274 80 33 51 1443 345 2042 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
2042 1712 1443 345 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2664 2696 6078 6110 2664
Size: 32 3382 32 2672
Fragments arranged by size:
3382 2672 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2825 3260 1316
Size: 1509 435 4174
Fragments arranged by size:
4174 1509 435
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2708 2708
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 2169 3443 1870
Size: 299 1274 4545
Fragments arranged by size:
4545 1274 299
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SrfI SwaI