Restriction map for
pFN21AB5811
(Circular) (Six-base) MAPSORT of: pFN21AB5811.seq Check: 6786 from: 1 to: 6127
pFN21AB5811 6127 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2353 3137 3937 278
Size: 53 83 186 936 817 784 800 2468
Fragments arranged by size:
2468 936 817 800 784 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2306 3470 1937
Size: 369 1164 4594
Fragments arranged by size:
4594 1164 369
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2557 3035 3163 3282 4190 5574 5842 1632
Size: 925 478 128 119 908 1384 268 1917
Fragments arranged by size:
1917 1384 925 908 478 268 128 119
AfeI AGC'GCT
Cuts at: 2780 2780
Size: 6127
AflII C'TTAA_G
Cuts at: 820 1017 3142 820
Size: 197 2125 3805
Fragments arranged by size:
3805 2125 197
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 3400 4856 3400
Size: 1456 4671
AleI CACnn'nnGTG
Cuts at: 1166 3353 1166
Size: 2187 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 3870 5442 3870
Size: 1572 4555
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2148 3436 3662 3814 5923 1493
Size: 655 1288 226 152 2109 1697
Fragments arranged by size:
2109 1697 1288 655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3381 3452 1870
Size: 73 1438 71 4545
Fragments arranged by size:
4545 1438 73 71
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2418 3446 1391
Size: 1027 1028 4072
Fragments arranged by size:
4072 1028 1027
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2284 3137 928
Size: 971 385 853 3918
Fragments arranged by size:
3918 971 853 385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2700 6122 2700
Size: 3422 2705
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2925 3152 4188 5344 1790
Size: 1135 227 1036 1156 2573
Fragments arranged by size:
2573 1156 1135 1036 227
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2940 3858 4816
Size: 416 53 83 399 24 1523 918 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1753 1523 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 3343 3437 4787 1239
Size: 1586 518 94 1350 2579
Fragments arranged by size:
2579 1586 1350 518 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 4252 5117 5415 2360
Size: 1892 865 298 3072
Fragments arranged by size:
3072 1892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3305 4790 882
Size: 617 1806 1485 2219
Fragments arranged by size:
2219 1806 1485 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2350 3134 3934 4316
Size: 53 83 186 936 817 784 800 382
Cuts at: 4316 4951 275
Size: 635 1451
Fragments arranged by size:
1451 936 817 800 784 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 2528 2558 1178
Size: 30 165 30 1071 30 24 30 4747
Fragments arranged by size:
4747 1071 165 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2789 2830 1186
Size: 1363 240 41 4483
Fragments arranged by size:
4483 1363 240 41
BseYI C'CCAG_C
Cuts at: 2354 2557 2968 5330 5826 2354
Size: 203 411 2362 496 2655
Fragments arranged by size:
2655 2362 496 411 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2092 2552 2579 1163
Size: 573 356 460 27 4711
Fragments arranged by size:
4711 573 460 356 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 4188 4273 5344 2436
Size: 1752 85 1071 3219
Fragments arranged by size:
3219 1752 1071 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2418 2436 2925 3152 3446 4188 4273
Size: 399 628 18 489 227 294 742 85
Cuts at: 4273 5344 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 628 489 399 294 227
85 18
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2090 2598 2643 3482 844
Size: 1246 508 45 839 3489
Fragments arranged by size:
3489 1246 839 508 45
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 4622 4796 65
Size: 1183 2017 1357 174 1396
Fragments arranged by size:
2017 1396 1357 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 4771 4958 1700
Size: 110 275 2686 187 2869
Fragments arranged by size:
2869 2686 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 2475 4931 2475
Size: 2456 3671
BstEII G'GTnAC_C
Cuts at: 1184 3421 1184
Size: 2237 3890
BstYI r'GATC_y
Cuts at: 1972 2538 2700 2793 3457 3824 4209 4226 5678
Size: 566 162 93 664 367 385 17 1452
Cuts at: 5678 5764 5776 6122 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 664 566 385 367 346 162
93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3374 3374
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 2004 2255 2835 3354 513
Size: 552 939 251 580 519 3286
Fragments arranged by size:
3286 939 580 552 519 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2209 3368 3643 4437 4457 2089
Size: 120 1159 275 794 20 3759
Fragments arranged by size:
3759 1159 794 275 120 20
ClaI AT'CG_AT
Cuts at: 1554 2370 1554
Size: 816 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2232 3238
Size: 54 1425 50 275 18 360 42 1006
Cuts at: 3238 3286 3574 4463 6092 8
Size: 48 288 889 1629 43
Fragments arranged by size:
1629 1425 1006 889 360 288 275 54
50 48 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3245 3301 4057 1353
Size: 1892 56 756 3423
Fragments arranged by size:
3423 1892 756 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2557 2825 3035 3163 3282 3343 3437
Size: 393 925 268 210 128 119 61 94
Cuts at: 3437 4190 4787 5574 5842 1239
Size: 753 597 787 268 1524
Fragments arranged by size:
1524 925 787 753 597 393 268 268
210 128 119 94 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 3142 2912
Size: 230 5897
EcoO109I rG'GnC_Cy
Cuts at: 2156 2632 2820 3302 3378 2156
Size: 476 188 482 76 4905
Fragments arranged by size:
4905 482 476 188 76
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2406 2782 2939 5274 1678
Size: 728 376 157 2335 2531
Fragments arranged by size:
2531 2335 728 376 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2204 2236 2320 2352 2504
Size: 32 135 32 632 32 84 32 152
Cuts at: 2504 2528 2536 2560 2840 2872 3386 3418 4768
Size: 24 8 24 280 32 514 32 1350
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 632 514 280 152 135 84
42 32 32 32 32 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2905 3471 3717 669
Size: 988 281 967 566 246 3079
Fragments arranged by size:
3079 988 967 566 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3153 3726 3153
Size: 573 5554
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 2446 2526 2709 5241 5425 1305
Size: 696 445 80 183 2532 184 2007
Fragments arranged by size:
2532 2007 696 445 184 183 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 3288 10
Size: 54 1425 343 360 1096 2849
Fragments arranged by size:
2849 1425 1096 360 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3353 4085 4444 4603 518
Size: 648 2187 732 359 159 2042
Fragments arranged by size:
2187 2042 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2248 2557 3097 3543 4220 5368 5613
Size: 512 130 309 540 446 677 1148 245
Cuts at: 5613 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 540 512 446 309 245
130
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4636
Fragments arranged by size:
4636 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2566 3334 3485 5030 1132
Size: 97 1337 768 151 1545 2229
Fragments arranged by size:
2229 1545 1337 768 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 3008 3321 1108
Size: 1566 334 313 3914
Fragments arranged by size:
3914 1566 334 313
PfoI T'CCnGG_A
Cuts at: 2802 2802
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3087 4196 4228 5709 5741 3055
Size: 32 1109 32 1481 32 3441
Fragments arranged by size:
3441 1481 1109 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2156 2632 2820 3378 2156
Size: 476 188 558 4905
Fragments arranged by size:
4905 558 476 188
PshAI GACnn'nnGTC
Cuts at: 1613 2284 2741 1613
Size: 671 457 4999
Fragments arranged by size:
4999 671 457
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2118 2557 3097 2118
Size: 439 540 5148
Fragments arranged by size:
5148 540 439
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SapI GCTCTTCn'nnn_
Cuts at: 3245 3245
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 3293 4375 6087 3293
Size: 1082 1712 3333
Fragments arranged by size:
3333 1712 1082
SexAI A'CCwGG_T
Cuts at: 2907 2907
Size: 6127
SfcI C'TryA_G
Cuts at: 826 1045 3475 4610 5291 5482 826
Size: 219 2430 1135 681 191 1471
Fragments arranged by size:
2430 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3142 4231 5132 5394 820
Size: 197 926 396 803 1089 901 262 1553
Fragments arranged by size:
1553 1089 926 901 803 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 2566 3334 3485 1132
Size: 1434 768 151 3774
Fragments arranged by size:
3774 1434 768 151
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 3067 3566 513
Size: 552 939 1063 499 3074
Fragments arranged by size:
3074 1063 939 552 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2487 3291 4373
Size: 274 80 33 51 1443 510 804 1082
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1082 804 510 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 3142 3174 6087 6119 2474
Size: 32 636 32 2913 32 2482
Fragments arranged by size:
2913 2482 636 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 2909 1316
Size: 906 687 4534
Fragments arranged by size:
4534 906 687
XbaI T'CTAG_A
Cuts at: 2048 3463 2048
Size: 1415 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2554 2870 3101 2554
Size: 316 231 5580
Fragments arranged by size:
5580 316 231
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2351 3135 3935 276
Size: 53 83 186 936 817 784 800 2468
Fragments arranged by size:
2468 936 817 800 784 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI