Restriction map for
pFN21AB5808
(Circular) (Six-base) MAPSORT of: pFN21AB5808.seq Check: 2504 from: 1 to: 6067
pFN21AB5808 6067 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 2633 3410 1937
Size: 325 371 777 4594
Fragments arranged by size:
4594 777 371 325
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3224 3374 4130 5514 5782 1632
Size: 1592 150 756 1384 268 1917
Fragments arranged by size:
1917 1592 1384 756 268 150
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 2376 2378 3126 4966 5943 1203
Size: 1173 2 748 1840 977 1327
Fragments arranged by size:
1840 1327 1173 977 748 2
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2959 3810 5382 2959
Size: 851 1572 3644
Fragments arranged by size:
3644 1572 851
ApaI G_GGCC'C
Cuts at: 2922 2922
Size: 6067
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 3392 1870
Size: 73 313 1136 4545
Fragments arranged by size:
4545 1136 313 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2224 3102 3388 4843
Size: 325 217 627 252 185 878 286 1455
Cuts at: 4843 618
Size: 1842
Fragments arranged by size:
1842 1455 878 627 325 286 252 217
185
BanII G_rGCy'C
Cuts at: 1391 2135 2555 2571 2766 2922 3045 3386 1391
Size: 744 420 16 195 156 123 341 4072
Fragments arranged by size:
4072 744 420 341 195 156 123 16
BbeI G_GCGC'C
Cuts at: 3106 3106
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2625 4678 136
Size: 107 122 71 631 605 953 2053 1525
Fragments arranged by size:
2053 1525 953 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 2129 2549 2765 2996 3484 2129
Size: 420 216 231 488 4712
Fragments arranged by size:
4712 488 420 231 216
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2101 2229 2922 4128 5284 1790
Size: 254 57 128 693 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 693 254 128 57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 3295 2197
Size: 1098 4969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2629 2987 3056 3272 4727 1239
Size: 1390 358 69 216 1455 2579
Fragments arranged by size:
2579 1455 1390 358 216 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4192 5057 5355 4192
Size: 865 298 4904
Fragments arranged by size:
4904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2115 3015 4730 882
Size: 617 616 900 1715 2219
Fragments arranged by size:
2219 1715 900 617 616
BsaAI yAC'GTr
Cuts at: 493 1204 2379 3858 493
Size: 711 1175 1479 2702
Fragments arranged by size:
2702 1479 1175 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3103 3874 4256 4891
Size: 53 83 186 936 1570 771 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1570 1451 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2493 2889 4500 4898 5172 5319 5730 2493
Size: 396 1611 398 274 147 411 2830
Fragments arranged by size:
2830 1611 411 398 396 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5842
Fragments arranged by size:
5842 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2244 3053 3271 1186
Size: 1045 13 809 218 3982
Fragments arranged by size:
3982 1045 809 218 13
BseYI C'CCAG_C
Cuts at: 2228 2623 2948 3113 3361 5270 5766 2228
Size: 395 325 165 248 1909 496 2529
Fragments arranged by size:
2529 1909 496 395 325 248 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2670 2684 2815 1163
Size: 573 934 14 131 4415
Fragments arranged by size:
4415 934 573 131 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2207 2571 4128 4213 5284 2207
Size: 364 1557 85 1071 2990
Fragments arranged by size:
2990 1557 1071 364 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2101 2135 2207 2229 2555 2571
Size: 399 254 57 34 72 22 326 16
Cuts at: 2571 2766 2922 3045 3386 4128 4213 5284 1391
Size: 195 156 123 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 326 254 195
156 123 85 72 57 34 22 16
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2063 3422 844
Size: 1219 1359 3489
Fragments arranged by size:
3489 1359 1219
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 2769 4562 4736 65
Size: 1183 1042 479 1793 174 1396
Fragments arranged by size:
1793 1396 1183 1042 479 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2439 2909 3105 4711 4898 1700
Size: 110 629 470 196 1606 187 2869
Fragments arranged by size:
2869 1606 629 470 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 3790
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2959 794
Size: 2165 3902
BstBI TT'CG_AA
Cuts at: 2457 4871 2457
Size: 2414 3653
BstEII G'GTnAC_C
Cuts at: 1184 2413 2529 1184
Size: 1229 116 4722
Fragments arranged by size:
4722 1229 116
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2176
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 2054 2671 3397 3764 4149 4166 5618 5704
Size: 82 617 726 367 385 17 1452 86
Cuts at: 5704 5716 6062 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 726 617 385 367 346 86
82 17 12
BstZ17I GTA'TAC
Cuts at: 2634 2634
Size: 6067
Bsu36I CC'TnA_GG
Cuts at: 3086 3086
Size: 6067
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3071 3583 4377 4397 3071
Size: 512 794 20 4741
Fragments arranged by size:
4741 794 512 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3514 4403
Size: 54 1425 50 275 18 177 1507 889
Cuts at: 4403 6032 8
Size: 1629 43
Fragments arranged by size:
1629 1507 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2559 3997 1353
Size: 1206 1438 3423
Fragments arranged by size:
3423 1438 1206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2103 2238 4654 5028 5174 387
Size: 1064 138 514 135 2416 374 146 1280
Fragments arranged by size:
2416 1280 1064 514 374 146 138 135
EcoICRI GAG'CTC
Cuts at: 2569 2569
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2629 2987 3056 3224 3272 3374 4130
Size: 393 997 358 69 168 48 102 756
Cuts at: 4130 4727 5514 5782 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 997 787 756 597 393 358 268
168 102 69 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2168 2168
Size: 6067
EcoO109I rG'GnC_Cy
Cuts at: 2048 2146 2919 3349 2048
Size: 98 773 430 4766
Fragments arranged by size:
4766 773 430 98
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
EcoRV GAT'ATC
Cuts at: 2812 2812
Size: 6067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2359 2290
Size: 69 5998
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 3003 3106 5214 1678
Size: 1325 103 2108 2531
Fragments arranged by size:
2531 2108 1325 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 2279 2311 2407
Size: 32 135 32 595 32 80 32 96
Cuts at: 2407 2439 3266 3298 4708 4740 4782 4814 1373
Size: 32 827 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1410 827 595 135 96 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 3102 3102
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 5181 5365 1305
Size: 1590 2286 184 2007
Fragments arranged by size:
2286 2007 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4068
Fragments arranged by size:
4068 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3303 4025 4384 4543 518
Size: 648 2137 722 359 159 2042
Fragments arranged by size:
2137 2042 722 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2623 2668 3483 4160 5308 5553 1606
Size: 1017 45 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 1017 815 677 245 45
NaeI GCC'GGC
Cuts at: 2441 2911 3107 2441
Size: 470 196 5401
Fragments arranged by size:
5401 470 196
NarI GG'CG_CC
Cuts at: 3103 3103
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4576
Fragments arranged by size:
4576 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 2439 2909 3105 2439
Size: 470 196 5401
Fragments arranged by size:
5401 470 196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2470 3130 3425 4970 1132
Size: 97 1241 660 295 1545 2229
Fragments arranged by size:
2229 1545 1241 660 295 97
PasI CC'CwG_GG
Cuts at: 1348 2044 2066 2093 1348
Size: 696 22 27 5322
Fragments arranged by size:
5322 696 27 22
PciI A'CATG_T
Cuts at: 3126 4966 3126
Size: 1840 4227
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PmlI CAC'GTG
Cuts at: 2379 2379
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2918 2918
Size: 6067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2357 2389 2357
Size: 32 6035
PstI C_TGCA'G
Cuts at: 830 3066 3419 830
Size: 2236 353 3478
Fragments arranged by size:
3478 2236 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2623 2668 2623
Size: 45 6022
SacI G_AGCT'C
Cuts at: 2571 2571
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2559 2559
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 2435 4315 6027 2435
Size: 1880 1712 2475
Fragments arranged by size:
2475 1880 1712
SfcI C'TryA_G
Cuts at: 826 1045 2444 3062 3415 4550 5231 5422 826
Size: 219 1399 618 353 1135 681 191 1471
Fragments arranged by size:
1471 1399 1135 681 618 353 219 191
SfoI GGC'GCC
Cuts at: 3104 3104
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 3105 1700
Size: 1405 4662
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4171 5072 5334 820
Size: 197 926 2228 901 262 1553
Fragments arranged by size:
2228 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6067
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2472 2577 3134 3506 513
Size: 552 939 165 303 105 557 372 3074
Fragments arranged by size:
3074 939 557 552 372 303 165 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 2433 4313
Size: 274 80 33 51 1443 396 60 1880
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1880 1712 1443 396 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 2933 1316
Size: 1617 4450
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2453 4196 2453
Size: 1743 4324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI FseI FspAI NruI NsiI PacI
PfoI PpuMI PspXI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI