Restriction map for
pFN21AB5807
(Circular) (Six-base) MAPSORT of: pFN21AB5807.seq Check: 3130 from: 1 to: 6121
pFN21AB5807 6121 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2021
Size: 6121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 2454 3442 2454
Size: 988 5133
AclI AA'CG_TT
Cuts at: 1563 3420 4248 4621 1563
Size: 1857 828 373 3063
Fragments arranged by size:
3063 1857 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2750 2771 4184 5568 5836 1632
Size: 574 544 21 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 574 544 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 3093 4850 3093
Size: 1757 4364
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2487 1184
Size: 1303 4818
AlwNI CAG_nnn'CTG
Cuts at: 3025 3864 5436 3025
Size: 839 1572 3710
Fragments arranged by size:
3710 1572 839
ApaLI G'TGCA_C
Cuts at: 3368 4178 5334 3368
Size: 810 1156 4155
Fragments arranged by size:
4155 1156 810
ApoI r'AATT_y
Cuts at: 1493 3430 3656 3808 5917 1493
Size: 1937 226 152 2109 1697
Fragments arranged by size:
2109 1937 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2454 3442 4897 618
Size: 325 217 627 667 988 1455 1842
Fragments arranged by size:
1842 1455 988 667 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 3006 3440 1391
Size: 663 952 434 4072
Fragments arranged by size:
4072 952 663 434
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3224 928
Size: 971 1325 3825
Fragments arranged by size:
3825 1325 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3389 3423 4312 4346 1903
Size: 34 1452 34 889 34 3678
Fragments arranged by size:
3678 1452 889 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4732 136
Size: 107 122 71 631 605 916 2144 1525
Fragments arranged by size:
2144 1525 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2994 6116 2994
Size: 3122 2999
BlpI GC'TnA_GC
Cuts at: 3050 3538 3050
Size: 488 5633
Bme1580I G_kGCm'C
Cuts at: 1790 3372 4182 5338 1790
Size: 1582 810 1156 2573
Fragments arranged by size:
2573 1582 1156 810
BmgBI CAC'GTC
Cuts at: 1318 3214 1318
Size: 1896 4225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3286 3852 4810
Size: 416 53 83 399 24 1869 566 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1869 1753 958 566 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2071 2432 2729 3047 3089 3101 3248
Size: 781 51 361 297 318 42 12 147
Cuts at: 3248 3281 3319 4781 1239
Size: 33 38 1462 2579
Fragments arranged by size:
2579 1462 781 361 318 297 147 51
42 38 33 12
Bpu10I CC'TnA_GC
Cuts at: 2843 2843
Size: 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3080 4784 882
Size: 617 1581 1704 2219
Fragments arranged by size:
2219 1704 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3273 3928 4310 4945
Size: 53 83 186 936 1740 655 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1740 1451 936 655 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 1178
Size: 30 165 30 1866 30 4000
Fragments arranged by size:
4000 1866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2089 2167 2429 2750 2780 2783 2951
Size: 837 66 78 262 321 30 3 168
Cuts at: 2951 3098 3161 3274 3277 3289 3322 1186
Size: 147 63 113 3 12 33 3985
Fragments arranged by size:
3985 837 321 262 168 147 113 78
66 63 33 30 12 3 3
BseYI C'CCAG_C
Cuts at: 2132 3045 3159 5324 5820 2132
Size: 913 114 2165 496 2433
Fragments arranged by size:
2433 2165 913 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 2996 1163
Size: 573 1075 185 4288
Fragments arranged by size:
4288 1075 573 185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2377 3571 4332 5360 1540
Size: 275 186 376 1194 761 1028 2301
Fragments arranged by size:
2301 1194 1028 761 376 275 186
BsiHKAI G_wGCw'C
Cuts at: 2410 3006 3372 4182 4267 5338 2410
Size: 596 366 810 85 1071 3193
Fragments arranged by size:
3193 1071 810 596 366 85
BsmI GAATG_Cn'
Cuts at: 2782 2850 3632 3725 2782
Size: 68 782 93 5178
Fragments arranged by size:
5178 782 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2410 3006 3372 3440 4182 4267
Size: 399 264 356 596 366 68 742 85
Cuts at: 4267 5338 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 596 399 366 356 264
85 68
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2640 3391 3476 844
Size: 1177 619 751 85 3489
Fragments arranged by size:
3489 1177 751 619 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2982 4008 1394
Size: 429 1159 1026 3507
Fragments arranged by size:
3507 1159 1026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 2762 3395 4181 5193 2762
Size: 633 786 1012 3690
Fragments arranged by size:
3690 1012 786 633
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2353 1184
Size: 1169 4952
BstXI CCAn_nnnn'nTGG
Cuts at: 2798 3361 2798
Size: 563 5558
BstYI r'GATC_y
Cuts at: 1972 2442 2994 3120 3201 3451 3818 4203 4220
Size: 470 552 126 81 250 367 385 17
Cuts at: 4220 5672 5758 5770 6116 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 552 470 385 367 346 250
126 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2519 2519
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2592 513
Size: 552 1527 4042
Fragments arranged by size:
4042 1527 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 2350 3637 4431 4451 2350
Size: 1287 794 20 4020
Fragments arranged by size:
4020 1287 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2589 2694
Size: 54 1425 50 275 18 279 480 105
Cuts at: 2694 2955 3568 4457 6086 8
Size: 261 613 889 1629 43
Fragments arranged by size:
1629 1425 889 613 480 279 275 261
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4051 1353
Size: 2698 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3004 3004
Size: 6121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2071 2206 2432 2729 2750 2771
Size: 393 388 51 135 226 297 21 21
Cuts at: 2771 3047 3089 3101 3248 3281 3319 4184 4781
Size: 276 42 12 147 33 38 865 597
Cuts at: 4781 5568 5836 1239
Size: 787 268 1524
Fragments arranged by size:
1524 865 787 597 393 388 297 276
268 226 147 135 51 42 38 33
21 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2732 3260 2396
Size: 336 528 5257
Fragments arranged by size:
5257 528 336
EcoO109I rG'GnC_Cy
Cuts at: 2118 2515 3403 2118
Size: 397 888 4836
Fragments arranged by size:
4836 888 397
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 2636 2669 4627 2636
Size: 33 1958 4130
Fragments arranged by size:
4130 1958 33
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 3079 3111 3314
Size: 32 135 32 1454 32 21 32 203
Cuts at: 3314 3346 4762 4794 4836 4868 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1454 1416 203 135 42 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 2458 3446 2458
Size: 988 5133
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 2957 10
Size: 54 1425 343 864 261 3174
Fragments arranged by size:
3174 1425 864 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2642 3365 4079 4438 4597 518
Size: 648 1476 723 714 359 159 2042
Fragments arranged by size:
2042 1476 723 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2467 2594 3112 3154 3168 3537 4214 5362
Size: 861 127 518 42 14 369 677 1148
Cuts at: 5362 5607 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 861 677 518 369 245 127
42 14
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5569
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3109 3479 5024 1132
Size: 97 1880 370 1545 2229
Fragments arranged by size:
2229 1880 1545 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PfoI T'CCnGG_A
Cuts at: 3123 3123
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 4190 4222 5703 5735 2903
Size: 32 1255 32 1481 32 3289
Fragments arranged by size:
3289 1481 1255 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2118 2515 3403 2118
Size: 397 888 4836
Fragments arranged by size:
4836 888 397
PshAI GACnn'nnGTC
Cuts at: 1613 2490 1613
Size: 877 5244
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 6089
PstI C_TGCA'G
Cuts at: 830 2018 2571 3042 3473 830
Size: 1188 553 471 431 3478
Fragments arranged by size:
3478 1188 553 471 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 3112 3154 3112
Size: 42 6079
SacI G_AGCT'C
Cuts at: 3006 3006
Size: 6121
SacII CC_GC'GG
Cuts at: 2595 2595
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2014 2488 2567 3038 3469 4604 5285
Size: 219 969 474 79 471 431 1135 681
Cuts at: 5285 5476 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 681 474 471 431 219
191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3109 3479 1132
Size: 1977 370 3774
Fragments arranged by size:
3774 1977 370
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2278 3354 3560 513
Size: 552 1213 1076 206 3074
Fragments arranged by size:
3074 1213 1076 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2786 3407 4148 4165 4318 4503
Size: 1255 702 504 621 741 17 153 185
Cuts at: 4503 4948 325
Size: 445 1498
Fragments arranged by size:
1498 1255 741 702 621 504 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3195 4367 6079
Size: 274 80 33 51 1443 1218 1172 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1443 1218 1172 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 6081 6113 3269
Size: 32 2780 32 3277
Fragments arranged by size:
3277 2780 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2704 2968 3022 3131 3299 2428
Size: 276 264 54 109 168 5250
Fragments arranged by size:
5250 276 264 168 109 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI