Restriction map for
pFN21AB5806
(Circular) (Six-base) MAPSORT of: pFN21AB5806.seq Check: 2007 from: 1 to: 6061
pFN21AB5806 6061 bp
With 182 enzymes: SgfI *
March 28, 2012 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3303 4124 5508 5776 1632
Size: 1671 821 1384 268 1917
Fragments arranged by size:
1917 1671 1384 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2612 2728 1166
Size: 1446 116 4499
Fragments arranged by size:
4499 1446 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 3326 3804 5376 3326
Size: 478 1572 4011
Fragments arranged by size:
4011 1572 478
ApaI G_GGCC'C
Cuts at: 2502 2502
Size: 6061
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 3044 3370 3596 3748 5857 1493
Size: 1551 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 1551 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3386 1870
Size: 73 964 479 4545
Fragments arranged by size:
4545 964 479 73
BamHI G'GATC_C
Cuts at: 3083 3391 3758 3083
Size: 308 367 5386
Fragments arranged by size:
5386 367 308
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2606 2712 3382 4837
Size: 325 217 627 372 447 106 670 1455
Cuts at: 4837 618
Size: 1842
Fragments arranged by size:
1842 1455 670 627 447 372 325 217
106
BanII G_rGCy'C
Cuts at: 1391 2334 2502 2546 3380 1391
Size: 943 168 44 834 4072
Fragments arranged by size:
4072 943 834 168 44
BbeI G_GCGC'C
Cuts at: 2716 2716
Size: 6061
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2848 928
Size: 971 949 4141
Fragments arranged by size:
4141 971 949
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 6061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2888 2922 4252 4286 1903
Size: 34 951 34 1330 34 3678
Fragments arranged by size:
3678 1330 951 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 3147 1548
Size: 1599 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2502 4122 5278 1790
Size: 712 1620 1156 2573
Fragments arranged by size:
2573 1620 1156 712
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 3089 3792
Size: 416 53 83 399 24 1134 538 703
Cuts at: 3792 4750 442
Size: 958 1753
Fragments arranged by size:
1753 1134 958 703 538 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 3073 4721 1239
Size: 875 959 1648 2579
Fragments arranged by size:
2579 1648 959 875
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 3079 4186 5051 5349 2354
Size: 725 1107 865 298 3066
Fragments arranged by size:
3066 1107 865 725 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2713 3868 4250
Size: 53 83 186 936 735 445 1155 382
Cuts at: 4250 4885 275
Size: 635 1451
Fragments arranged by size:
1451 1155 936 735 635 445 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 4666
Fragments arranged by size:
4666 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 2122 1186
Size: 933 3 5125
Fragments arranged by size:
5125 933 3
BseYI C'CCAG_C
Cuts at: 2404 2501 5264 5760 2404
Size: 97 2763 496 2705
Fragments arranged by size:
2763 2705 496 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 3201 1163
Size: 573 600 865 4023
Fragments arranged by size:
4023 865 600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2256 3511 4272 5300 1540
Size: 275 186 255 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 4122 4207 5278 4122
Size: 85 1071 4905
Fragments arranged by size:
4905 1071 85
BsmI GAATG_Cn'
Cuts at: 2475 3572 3665 2475
Size: 1097 93 4871
Fragments arranged by size:
4871 1097 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2502 2546 3380 4122 4207 5278
Size: 399 544 168 44 834 742 85 1071
Cuts at: 5278 1391
Size: 2174
Fragments arranged by size:
2174 1071 834 742 544 399 168 85
44
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 2445 3950 5680 2445
Size: 1505 1730 2826
Fragments arranged by size:
2826 1730 1505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3416 844
Size: 1514 1058 3489
Fragments arranged by size:
3489 1514 1058
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 3263 3948 1394
Size: 429 386 1054 685 3507
Fragments arranged by size:
3507 1054 685 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 2694 4556 4730 65
Size: 1183 1446 1862 174 1396
Fragments arranged by size:
1862 1446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 4705 4892 1700
Size: 110 346 2549 187 2869
Fragments arranged by size:
2869 2549 346 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3179 794
Size: 2385 3676
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 4556
BstYI r'GATC_y
Cuts at: 1972 2316 3083 3391 3758 4143 4160 5612 5698
Size: 344 767 308 367 385 17 1452 86
Cuts at: 5698 5710 6056 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 767 385 367 346 344 308
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2323 2452 2520 513
Size: 552 1258 129 68 4054
Fragments arranged by size:
4054 1258 552 129 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2514 2578
Size: 54 1425 50 275 18 423 261 64
Cuts at: 2578 2650 3274 3508 4397 6026 8
Size: 72 624 234 889 1629 43
Fragments arranged by size:
1629 1425 889 624 423 275 261 234
72 64 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2253 3508 1537
Size: 275 441 1255 4090
Fragments arranged by size:
4090 1255 441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2593 2761 3991 1353
Size: 1240 168 1230 3423
Fragments arranged by size:
3423 1240 1230 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3273 3281 4648 5022 5168 387
Size: 1064 138 1684 8 1367 374 146 1280
Fragments arranged by size:
1684 1367 1280 1064 374 146 138 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 3073 3303 4124 4721 5508 5776
Size: 393 482 959 230 821 597 787 268
Cuts at: 5776 1239
Size: 1524
Fragments arranged by size:
1524 959 821 787 597 482 393 268
230
EcoO109I rG'GnC_Cy
Cuts at: 2498 2498
Size: 6061
EcoRI G'AATT_C
Cuts at: 3044 3370 3748 3044
Size: 326 378 5357
Fragments arranged by size:
5357 378 326
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2369 2716 5208 1678
Size: 691 347 2492 2531
Fragments arranged by size:
2531 2492 691 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2258 2290 4702
Size: 32 135 32 584 32 70 32 2412
Cuts at: 4702 4734 4776 4808 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2412 584 135 70 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2620 3405 3651 669
Size: 988 281 72 610 785 246 3079
Fragments arranged by size:
3079 988 785 610 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2712 2712
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 3276 10
Size: 54 1425 343 748 696 2795
Fragments arranged by size:
2795 1425 748 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 2728 4019 4378 4537 518
Size: 648 1446 116 1291 359 159 2042
Fragments arranged by size:
2042 1446 1291 648 359 159 116
MspA1I CmG'CkG
Cuts at: 1606 2522 2793 3477 4154 5302 5547 1606
Size: 916 271 684 677 1148 245 2120
Fragments arranged by size:
2120 1148 916 684 677 271 245
NarI GG'CG_CC
Cuts at: 2713 2713
Size: 6061
NcoI C'CATG_G
Cuts at: 513 1065 2452 513
Size: 552 1387 4122
Fragments arranged by size:
4122 1387 552
NdeI CA'TA_TG
Cuts at: 387 2979 387
Size: 2592 3469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3287 3419 4964 1132
Size: 97 2058 132 1545 2229
Fragments arranged by size:
2229 2058 1545 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PfoI T'CCnGG_A
Cuts at: 2020 2020
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2498 2498
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SacII CC_GC'GG
Cuts at: 2523 2523
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SapI GCTCTTCn'nnn_
Cuts at: 2761 2761
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 2306 4309 6021 2306
Size: 2003 1712 2346
Fragments arranged by size:
2346 2003 1712
SfcI C'TryA_G
Cuts at: 826 1045 3409 4544 5225 5416 826
Size: 219 2364 1135 681 191 1471
Fragments arranged by size:
2364 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2714 2714
Size: 6061
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 3058 4165 5066 5328 820
Size: 197 926 426 689 1107 901 262 1553
Fragments arranged by size:
1553 1107 926 901 689 426 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 2026 152
Size: 1874 4187
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2452 2581 2877 3277 3500 513
Size: 552 1387 129 296 400 223 3074
Fragments arranged by size:
3074 1387 552 400 296 223 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2490 4088 4105 4258 4443 4888 325
Size: 1255 910 1598 17 153 185 445 1498
Fragments arranged by size:
1598 1498 1255 910 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2304 4307
Size: 274 80 33 51 1443 222 105 2003
Cuts at: 4307 6019 96
Size: 1712 138
Fragments arranged by size:
2003 1712 1443 274 222 138 105 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2543 2575 6021 6053 2204
Size: 32 307 32 3446 32 2212
Fragments arranged by size:
3446 2212 307 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3106 3397 3106
Size: 291 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2977 2509
Size: 468 5593
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SrfI SwaI