Restriction map for
pFN21AB5805
(Circular) (Six-base) MAPSORT of: pFN21AB5805.seq Check: 9853 from: 1 to: 6118
pFN21AB5805 6118 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2954 3928 278
Size: 53 83 186 936 1418 974 2468
Fragments arranged by size:
2468 1418 974 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4181 5565 5833 1632
Size: 2549 1384 268 1917
Fragments arranged by size:
2549 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 3156 5017 5994 1203
Size: 1953 1861 977 1327
Fragments arranged by size:
1953 1861 1327 977
AgeI A'CCGG_T
Cuts at: 3333 4949 3333
Size: 1616 4502
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 3861 5433 3861
Size: 1572 4546
ApaI G_GGCC'C
Cuts at: 2548 3044 2548
Size: 496 5622
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 3427 3653 3805 5914 1493
Size: 1934 226 152 2109 1697
Fragments arranged by size:
2109 1934 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2961 2961
Size: 6118
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 2548 2903 3043 3320 3340 3443
Size: 73 206 399 355 140 277 20 103
Cuts at: 3443 1870
Size: 4545
Fragments arranged by size:
4545 399 355 277 206 140 103 73
20
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2291 2514 2836 2881
Size: 325 217 627 248 256 223 322 45
Cuts at: 2881 3097 3439 4894 618
Size: 216 342 1455 1842
Fragments arranged by size:
1842 1455 627 342 325 322 256 248
223 217 216 45
BanII G_rGCy'C
Cuts at: 1391 2132 2156 2198 2548 2765 2897 3044 3360
Size: 741 24 42 350 217 132 147 316
Cuts at: 3360 3437 1391
Size: 77 4072
Fragments arranged by size:
4072 741 350 316 217 147 132 77
42 24
BbeI G_GCGC'C
Cuts at: 2039 2295 2518 2840 2885 2039
Size: 256 223 322 45 5272
Fragments arranged by size:
5272 322 256 223 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2168 3061 3083
Size: 107 122 71 631 605 496 893 22
Cuts at: 3083 4729 136
Size: 1646 1525
Fragments arranged by size:
1646 1525 893 631 605 496 122 107
71 22
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 2891 3535 2891
Size: 644 5474
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2548 2636 3044 3100 4179 5335 1790
Size: 276 482 88 408 56 1079 1156 2573
Fragments arranged by size:
2573 1156 1079 482 408 276 88 56
BmgBI CAC'GTC
Cuts at: 1318 3015 3159 1318
Size: 1697 144 4277
Fragments arranged by size:
4277 1697 144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3849 4807 442
Size: 416 53 83 399 24 2432 958 1753
Fragments arranged by size:
2432 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2456 1056
Size: 1400 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2813 4778 1239
Size: 1574 1965 2579
Fragments arranged by size:
2579 1965 1574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 2504 3008 4243 5108 5406 2375
Size: 129 504 1235 865 298 3087
Fragments arranged by size:
3087 1235 865 504 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 2633 3227 4781 882
Size: 617 992 142 594 1554 2219
Fragments arranged by size:
2219 1554 992 617 594 142
BsaAI yAC'GTr
Cuts at: 493 1204 2674 3909 493
Size: 711 1470 1235 2702
Fragments arranged by size:
2702 1470 1235 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2123 2217 2292
Size: 53 83 186 936 503 87 94 75
Cuts at: 2292 2376 2515 2837 2882 2933 2951 3402 3925
Size: 84 139 322 45 51 18 451 523
Cuts at: 3925 4307 4942 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 523 503 451 382 322
186 139 94 87 84 83 75 53
51 45 18
BsaWI w'CCGG_w
Cuts at: 3333 4551 4949 5223 5370 5781 3333
Size: 1218 398 274 147 411 3670
Fragments arranged by size:
3670 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2831 1186
Size: 934 711 4473
Fragments arranged by size:
4473 934 711
BseYI C'CCAG_C
Cuts at: 3136 5321 5817 3136
Size: 2185 496 3437
Fragments arranged by size:
3437 2185 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2654 3251 3367 1163
Size: 573 918 597 116 3914
Fragments arranged by size:
3914 918 597 573 116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2214 2706 3075 3246 3568 4329
Size: 275 186 213 492 369 171 322 761
Cuts at: 4329 5357 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 492 369 322 275 213
186 171
BsiHKAI G_wGCw'C
Cuts at: 2412 2702 4179 4264 5335 2412
Size: 290 1477 85 1071 3195
Fragments arranged by size:
3195 1477 1071 290 85
BsiWI C'GTAC_G
Cuts at: 2310 2310
Size: 6118
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
BsmBI CGTCTCn'nnnn_
Cuts at: 2958 2958
Size: 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2132 2156 2198 2412 2548 2636
Size: 399 276 66 24 42 214 136 88
Cuts at: 2636 2702 2765 2897 3044 3100 3360 3437 4179
Size: 66 63 132 147 56 260 77 742
Cuts at: 4179 4264 5335 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 260 214 147
136 132 88 85 77 66 66 63
56 42 24
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 3319 4005 1394
Size: 429 1004 492 686 3507
Fragments arranged by size:
3507 1004 686 492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2183 2234 2279 2288 2511 2870 3082
Size: 110 373 51 45 9 223 359 212
Cuts at: 3082 3333 4762 4949 1700
Size: 251 1429 187 2869
Fragments arranged by size:
2869 1429 373 359 251 223 212 187
110 51 45 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 2114 2507 2961 3309 1925
Size: 189 393 454 348 4734
Fragments arranged by size:
4734 454 393 348 189
BssSI C'ACGA_G
Cuts at: 2059 2695 4178 5190 2059
Size: 636 1483 1012 2987
Fragments arranged by size:
2987 1483 1012 636
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113
Size: 1476 367 385 17 1452 86 12 346
Cuts at: 6113 1972
Size: 1977
Fragments arranged by size:
1977 1476 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2379 2917 3069 3246 3387 513
Size: 552 1314 538 152 177 141 3244
Fragments arranged by size:
3244 1314 552 538 177 152 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 3634 4428 4448 3634
Size: 794 20 5304
Fragments arranged by size:
5304 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2211 2382
Size: 54 1425 50 275 18 348 33 171
Cuts at: 2382 2703 2923 3000 3072 3243 3285 3565 4454
Size: 321 220 77 72 171 42 280 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 348 321 280 275 220
171 171 77 72 54 50 43 42
33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2211 2703 3072 3243 3565 1537
Size: 275 399 492 369 171 322 4090
Fragments arranged by size:
4090 492 399 369 322 275 171
EarI CTCTTCn'nnn_
Cuts at: 1353 4048 1353
Size: 2695 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2813 4181 4778 5565 5833 1239
Size: 393 1181 1368 597 787 268 1524
Fragments arranged by size:
1524 1368 1181 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2480 2522 2030
Size: 450 42 5626
Fragments arranged by size:
5626 450 42
EcoO109I rG'GnC_Cy
Cuts at: 2545 2743 2830 3262 2545
Size: 198 87 432 5401
Fragments arranged by size:
5401 432 198 87
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2039 2054 2295 2518 2840 2885 3209 5265
Size: 361 15 241 223 322 45 324 2056
Cuts at: 5265 1678
Size: 2531
Fragments arranged by size:
2531 2056 361 324 322 241 223 45
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373
Size: 32 135 32 3187 32 42 32 2626
Fragments arranged by size:
3187 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2723 748
Size: 1975 4143
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 2035 2291 2514 2836 2881 2035
Size: 256 223 322 45 5272
Fragments arranged by size:
5272 322 256 223 45
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 2997 5232 5416 1305
Size: 1386 306 2235 184 2007
Fragments arranged by size:
2235 2007 1386 306 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 2468 4076 4435 4594 518
Size: 648 1116 186 1608 359 159 2042
Fragments arranged by size:
2042 1608 1116 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2887 2919 3071 3133 3248 3534 4211
Size: 594 687 32 152 62 115 286 677
Cuts at: 4211 5359 5604 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 594 286 245 152
115 62 32
NaeI GCC'GGC
Cuts at: 2236 2513 3084 2236
Size: 277 571 5270
Fragments arranged by size:
5270 571 277
NarI GG'CG_CC
Cuts at: 2036 2292 2515 2837 2882 2036
Size: 256 223 322 45 5272
Fragments arranged by size:
5272 322 256 223 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5566
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NgoMIV G'CCGG_C
Cuts at: 2234 2511 3082 2234
Size: 277 571 5270
Fragments arranged by size:
5270 571 277
NheI G'CTAG_C
Cuts at: 1052 2452 1052
Size: 1400 4718
NotI GC'GGCC_GC
Cuts at: 3072 3243 3565 3072
Size: 171 322 5625
Fragments arranged by size:
5625 322 171
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2938 1348
Size: 1590 4528
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2991 1108
Size: 1883 4235
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PmlI CAC'GTG
Cuts at: 2674 2674
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 2743
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 2610 1613
Size: 997 5121
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2544 3040 2544
Size: 496 5622
PspXI vC'TCGA_Gb
Cuts at: 3320 3320
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2205 3213 3470 830
Size: 1375 1008 257 3478
Fragments arranged by size:
3478 1375 1008 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
SacII CC_GC'GG
Cuts at: 2920 3072 3249 2920
Size: 152 177 5789
Fragments arranged by size:
5789 177 152
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SanDI GG'GwC_CC
Cuts at: 2743 2743
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 2207 4366 6078 2207
Size: 2159 1712 2247
Fragments arranged by size:
2247 2159 1712
SexAI A'CCwGG_T
Cuts at: 2492 2492
Size: 6118
SfcI C'TryA_G
Cuts at: 826 1045 2201 3209 3466 4601 5282 5473 826
Size: 219 1156 1008 257 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 1008 681 257 219 191
SfoI GGC'GCC
Cuts at: 2037 2293 2516 2838 2883 2037
Size: 256 223 322 45 5272
Fragments arranged by size:
5272 322 256 223 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 2511 1700
Size: 811 5307
SmaI CCC'GGG
Cuts at: 1872 2151 3045 3342 3445 1872
Size: 279 894 297 103 4545
Fragments arranged by size:
4545 894 297 279 103
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2390 2519 3023 3320 4222 5123
Size: 197 926 447 129 504 297 902 901
Cuts at: 5123 5385 820
Size: 262 1553
Fragments arranged by size:
1553 926 902 901 504 447 297 262
197 129
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SrfI GCCC'GGGC
Cuts at: 3045 3045
Size: 6118
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2396 1517
Size: 879 5239
StyI C'CwwG_G
Cuts at: 513 1065 2433 3557 513
Size: 552 1368 1124 3074
Fragments arranged by size:
3074 1368 1124 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 4145 4162 4315 4500 4945 325
Size: 1255 915 1650 17 153 185 445 1498
Fragments arranged by size:
1650 1498 1255 915 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2205 4364 6076
Size: 274 80 33 51 1443 228 2159 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
2159 1712 1443 274 228 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 2735 2767 3320 3352 6078 6110 2681
Size: 32 22 32 553 32 2726 32 2689
Fragments arranged by size:
2726 2689 553 32 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 1316 2857 1316
Size: 1541 4577
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 3320 1943
Size: 1377 4741
XmaI C'CCGG_G
Cuts at: 1870 2149 3043 3340 3443 1870
Size: 279 894 297 103 4545
Fragments arranged by size:
4545 894 297 279 103
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2952 3926 276
Size: 53 83 186 936 1418 974 2468
Fragments arranged by size:
2468 1418 974 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PsrI PvuII
RsrII SacI SapI SfiI SwaI XcmI