Restriction map for
pFN21AB5796
(Circular) (Six-base) MAPSORT of: pFN21AB5796.seq Check: 1609 from: 1 to: 7045
pFN21AB5796 7045 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4388 1937
Size: 2451 4594
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3435 4184 4321 5108 6492 6760 1632
Size: 1803 749 137 787 1384 268 1917
Fragments arranged by size:
1917 1803 1384 787 749 268 137
AfeI AGC'GCT
Cuts at: 3839 3839
Size: 7045
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 3121 5944 6921 1203
Size: 1918 2823 977 1327
Fragments arranged by size:
2823 1918 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 2163 2511 2529 2811 2826 1166
Size: 997 348 18 282 15 5385
Fragments arranged by size:
5385 997 348 282 18 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 7013
AlwNI CAG_nnn'CTG
Cuts at: 2024 3277 4788 6360 2024
Size: 1253 1511 1572 2709
Fragments arranged by size:
2709 1572 1511 1253
ApaI G_GGCC'C
Cuts at: 3664 3664
Size: 7045
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2476 4354 4580 4732 6841 1493
Size: 983 1878 226 152 2109 1697
Fragments arranged by size:
2109 1878 1697 983 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 4370 1870
Size: 73 2427 4545
Fragments arranged by size:
4545 2427 73
BamHI G'GATC_C
Cuts at: 3633 4080 4375 4742 3633
Size: 447 295 367 5936
Fragments arranged by size:
5936 447 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2889 3932 4154 4366
Size: 325 217 627 525 577 1043 222 212
Cuts at: 4366 5821 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1043 627 577 525 325 222
217 212
BanII G_rGCy'C
Cuts at: 1391 2122 2275 2361 2610 3080 3664 4119 4134
Size: 731 153 86 249 470 584 455 15
Cuts at: 4134 4218 4364 1391
Size: 84 146 4072
Fragments arranged by size:
4072 731 584 470 455 249 153 146
86 84 15
BbeI G_GCGC'C
Cuts at: 2316 3936 2316
Size: 1620 5425
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3040 3186 3637 4107 928
Size: 971 1141 146 451 470 3866
Fragments arranged by size:
3866 1141 971 470 451 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4937 6147 4937
Size: 1210 5835
BclI T'GATC_A
Cuts at: 1548 2815 1548
Size: 1267 5778
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 3668 5656
Size: 107 122 71 631 605 614 1382 1988
Cuts at: 5656 136
Size: 1525
Fragments arranged by size:
1988 1525 1382 631 614 605 122 107
71
BglII A'GATC_T
Cuts at: 7040 7040
Size: 7045
BlpI GC'TnA_GC
Cuts at: 3692 4462 3692
Size: 770 6275
Bme1580I G_kGCm'C
Cuts at: 1790 2157 2505 3664 3814 3928 5106 6262 1790
Size: 367 348 1159 150 114 1178 1156 2573
Fragments arranged by size:
2573 1178 1159 1156 367 348 150 114
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 3193 4776
Size: 416 53 83 399 24 1403 373 1583
Cuts at: 4776 5734 442
Size: 958 1753
Fragments arranged by size:
1753 1583 1403 958 416 399 373 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3088 3215 4344 5705 1239
Size: 1849 127 1129 1361 2579
Fragments arranged by size:
2579 1849 1361 1129 127
Bpu10I CC'TnA_GC
Cuts at: 2600 2600
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 3479 3623 4070 5170 6035 6333 2292
Size: 1187 144 447 1100 865 298 3004
Fragments arranged by size:
3004 1187 1100 865 447 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2448 2732 5708 882
Size: 617 949 284 2976 2219
Fragments arranged by size:
2976 2219 949 617 284
BsaAI yAC'GTr
Cuts at: 493 1204 3061 3122 3478 4836 493
Size: 711 1857 61 356 1358 2702
Fragments arranged by size:
2702 1857 1358 711 356 61
BsaBI GATnn'nnATC
Cuts at: 7039 7039
Size: 7045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 3933 4852 5234
Size: 53 83 186 936 780 1620 919 382
Cuts at: 5234 5869 275
Size: 635 1451
Fragments arranged by size:
1620 1451 936 919 780 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2185 3324 3630 5478 5876 6150 6297 6708 2185
Size: 1139 306 1848 398 274 147 411 2522
Fragments arranged by size:
2522 1848 1139 411 398 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4205 4235 1178
Size: 30 165 30 2802 30 3988
Fragments arranged by size:
3988 2802 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2715 1186
Size: 1529 5516
BseYI C'CCAG_C
Cuts at: 2625 6248 6744 2625
Size: 3623 496 2926
Fragments arranged by size:
3623 2926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6472
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 4495 5256 6284 1540
Size: 275 186 1225 1269 761 1028 2301
Fragments arranged by size:
2301 1269 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2122 2361 2969 4119 4218 5106 5191 6262 2122
Size: 239 608 1150 99 888 85 1071 2905
Fragments arranged by size:
2905 1150 1071 888 608 239 99 85
BsmI GAATG_Cn'
Cuts at: 4556 4649 4556
Size: 93 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2157 2275 2361 2505 2610 2969
Size: 399 332 35 118 86 144 105 359
Cuts at: 2969 3080 3664 3814 3928 4119 4134 4218 4364
Size: 111 584 150 114 191 15 84 146
Cuts at: 4364 5106 5191 6262 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 399 359 332 191
150 146 144 118 114 111 105 86
85 84 35 15
BspEI T'CCGG_A
Cuts at: 2185 3324 3630 6708 2185
Size: 1139 306 3078 2522
Fragments arranged by size:
3078 2522 1139 306
BspHI T'CATG_A
Cuts at: 2383 2698 4934 6664 2383
Size: 315 2236 1730 2764
Fragments arranged by size:
2764 2236 1730 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 3788 4239 4400 844
Size: 1832 1112 451 161 3489
Fragments arranged by size:
3489 1832 1112 451 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 4275 4932 1394
Size: 429 1294 1158 657 3507
Fragments arranged by size:
3507 1294 1158 657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5540 5714 65
Size: 1183 4292 174 1396
Fragments arranged by size:
4292 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2886 3221 3225 5689 5876 1700
Size: 110 1076 335 4 2464 187 2869
Fragments arranged by size:
2869 2464 1076 335 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 5105 6117 5105
Size: 1012 6033
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstXI CCAn_nnnn'nTGG
Cuts at: 2165 2369 2402 2531 2681 2714 4141 2165
Size: 204 33 129 150 33 1427 5069
Fragments arranged by size:
5069 1427 204 150 129 33 33
BstYI r'GATC_y
Cuts at: 1972 3423 3633 4080 4375 4742 5127 5144 6596
Size: 1451 210 447 295 367 385 17 1452
Cuts at: 6596 6682 6694 7040 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1451 447 385 367 346 295
210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3665 3665
Size: 7045
BtgI C'CryG_G
Cuts at: 513 1065 2004 2275 2449 2509 2527 2548 2647
Size: 552 939 271 174 60 18 21 99
Cuts at: 2647 2680 2839 2920 2947 3071 3564 3798 513
Size: 33 159 81 27 124 493 234 3760
Fragments arranged by size:
3760 939 552 493 271 234 174 159
124 99 81 60 33 27 21 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3487 3736 487
Size: 683 56 2261 249 3796
Fragments arranged by size:
3796 2261 683 249 56
BtsI GCAGTG_nn'
Cuts at: 4561 5355 5375 4561
Size: 794 20 6231
Fragments arranged by size:
6231 794 20
ClaI AT'CG_AT
Cuts at: 1554 4293 1554
Size: 2739 4306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2272 2307 529
Size: 35 1708 35 5267
Fragments arranged by size:
5267 1708 35 35
DraI TTT'AAA
Cuts at: 4349 4349
Size: 7045
DraIII CAC_nnn'GTG
Cuts at: 2152 2152
Size: 7045
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3223 3561 3567
Size: 54 1425 50 275 18 1393 338 6
Cuts at: 3567 4492 5381 7010 8
Size: 925 889 1629 43
Fragments arranged by size:
1629 1425 1393 925 889 338 275 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3223 4492 1537
Size: 275 1411 1269 4090
Fragments arranged by size:
4090 1411 1269 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3272 3632 3791 3797 3878 4323 4975 1353
Size: 1919 360 159 6 81 445 652 3423
Fragments arranged by size:
3423 1919 652 445 360 159 81 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2120 2359 4117 4216 2120
Size: 239 1758 99 4949
Fragments arranged by size:
4949 1758 239 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3088 3215 3435 4184 4321 4344 5108
Size: 393 1456 127 220 749 137 23 764
Cuts at: 5108 5705 6492 6760 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1456 787 764 749 597 393 268
220 137 127 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3302 3302
Size: 7045
EcoO109I rG'GnC_Cy
Cuts at: 2391 2535 2688 2766 3661 3679 3726 2391
Size: 144 153 78 895 18 47 5710
Fragments arranged by size:
5710 895 153 144 78 47 18
EcoRI G'AATT_C
Cuts at: 4354 4732 4354
Size: 378 6667
EcoRV GAT'ATC
Cuts at: 2784 3160 2784
Size: 376 6669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3310 3310
Size: 7045
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 2316 3841 3936 4037 6192 1678
Size: 638 1525 95 101 2155 2531
Fragments arranged by size:
2531 2155 1525 638 101 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2465 2497 3884
Size: 32 135 32 428 32 433 32 1387
Cuts at: 3884 3916 5686 5718 5760 5792 1373
Size: 32 1770 32 42 32 2626
Fragments arranged by size:
2626 1770 1387 433 428 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4389 4635 669
Size: 988 281 2451 246 3079
Fragments arranged by size:
3079 2451 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7045
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KasI G'GCGC_C
Cuts at: 2312 3932 2312
Size: 1620 5425
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 4644 4644
Size: 7045
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2386 2635 6159 6343 1305
Size: 1081 249 3524 184 2007
Fragments arranged by size:
3524 2007 1081 249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3563 10
Size: 54 1425 343 1731 3492
Fragments arranged by size:
3492 1731 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 2385 2511 2529 2679 2712 2811
Size: 648 997 222 126 18 150 33 99
Cuts at: 2811 2826 3131 3389 3395 3476 3569 3770 3911
Size: 15 305 258 6 81 93 201 141
Cuts at: 3911 5003 5362 5521 518
Size: 1092 359 159 2042
Fragments arranged by size:
2042 1092 997 648 359 305 258 222
201 159 150 141 126 99 93 81
33 18 15 6
MspA1I CmG'CkG
Cuts at: 1606 3235 3800 4237 4461 5138 6286 6531 1606
Size: 1629 565 437 224 677 1148 245 2120
Fragments arranged by size:
2120 1629 1148 677 565 437 245 224
NaeI GCC'GGC
Cuts at: 3227 3227
Size: 7045
NarI GG'CG_CC
Cuts at: 2313 3933 2313
Size: 1620 5425
NcoI C'CATG_G
Cuts at: 513 1065 2004 2275 2449 2527 2548 2680 2839
Size: 552 939 271 174 78 21 132 159
Cuts at: 2839 2947 3564 513
Size: 108 617 3994
Fragments arranged by size:
3994 939 617 552 271 174 159 132
108 78 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7045
NgoMIV G'CCGG_C
Cuts at: 3225 3225
Size: 7045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 4403 5948 1132
Size: 97 3174 1545 2229
Fragments arranged by size:
3174 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3508 3730 3928 1348
Size: 2160 222 198 4465
Fragments arranged by size:
4465 2160 222 198
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3343 1108
Size: 2235 4810
PfoI T'CCnGG_A
Cuts at: 2996 3312 3711 2996
Size: 316 399 6330
Fragments arranged by size:
6330 399 316
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PmlI CAC'GTG
Cuts at: 3122 3122
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5114 5146 6627 6659 4205
Size: 32 877 32 1481 32 4591
Fragments arranged by size:
4591 1481 877 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2391 2535 2688 2766 3679 3726 2391
Size: 144 153 78 913 47 5710
Fragments arranged by size:
5710 913 153 144 78 47
PshAI GACnn'nnGTC
Cuts at: 1613 3707 1613
Size: 2094 4951
PsiI TTA'TAA
Cuts at: 4615 4615
Size: 7045
PspOMI G'GGCC_C
Cuts at: 3660 3660
Size: 7045
PstI C_TGCA'G
Cuts at: 830 4129 4236 4397 830
Size: 3299 107 161 3478
Fragments arranged by size:
3478 3299 161 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 4237 4237
Size: 7045
SacI G_AGCT'C
Cuts at: 2122 2361 4119 4218 2122
Size: 239 1758 99 4949
Fragments arranged by size:
4949 1758 239 99
SacII CC_GC'GG
Cuts at: 3801 3801
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SanDI GG'GwC_CC
Cuts at: 2391 3726 2391
Size: 1335 5710
SbfI CC_TGCA'GG
Cuts at: 4397 4397
Size: 7045
ScaI AGT'ACT
Cuts at: 3980 5293 7005 3980
Size: 1313 1712 4020
Fragments arranged by size:
4020 1712 1313
SexAI A'CCwGG_T
Cuts at: 3996 3996
Size: 7045
SfcI C'TryA_G
Cuts at: 826 1045 2563 4125 4232 4393 5528 6209 6400
Size: 219 1518 1562 107 161 1135 681 191
Cuts at: 6400 826
Size: 1471
Fragments arranged by size:
1562 1518 1471 1135 681 219 191 161
107
SfoI GGC'GCC
Cuts at: 2314 3934 2314
Size: 1620 5425
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 4372 1872
Size: 2500 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 3494 3638 4049 5149 6050
Size: 197 926 364 1187 144 411 1100 901
Cuts at: 6050 6312 820
Size: 262 1553
Fragments arranged by size:
1553 1187 1100 926 901 411 364 262
197 144
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 4047 1517
Size: 2530 4515
StyI C'CwwG_G
Cuts at: 513 1065 2004 2275 2449 2527 2548 2680 2809
Size: 552 939 271 174 78 21 132 129
Cuts at: 2809 2839 2947 3342 3450 3564 4484 513
Size: 30 108 395 108 114 920 3074
Fragments arranged by size:
3074 939 920 552 395 271 174 132
129 114 108 108 78 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3084 3087 3143 5072 5089 5242 5427
Size: 1255 1504 3 56 1929 17 153 185
Cuts at: 5427 5872 325
Size: 445 1498
Fragments arranged by size:
1929 1504 1498 1255 445 185 153 56
17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3978 5291 7003
Size: 274 80 33 51 1443 2001 1313 1712
Cuts at: 7003 96
Size: 138
Fragments arranged by size:
2001 1712 1443 1313 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 7005 7037 2606
Size: 32 4367 32 2614
Fragments arranged by size:
4367 2614 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2957 3097 1316
Size: 1641 140 5264
Fragments arranged by size:
5264 1641 140
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 3571 2492
Size: 1079 5966
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7045
XmaI C'CCGG_G
Cuts at: 1870 4370 1870
Size: 2500 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 5174 3851
Size: 1323 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SapI SfiI SrfI SwaI