Restriction map for
pFN21AB5793
(Circular) (Six-base) MAPSORT of: pFN21AB5793.seq Check: 7747 from: 1 to: 7198
pFN21AB5793 7198 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5008 278
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2093 3243 4541 1937
Size: 156 1150 1298 4594
Fragments arranged by size:
4594 1298 1150 156
Acc65I G'GTAC_C
Cuts at: 4146 4519 4146
Size: 373 6825
AclI AA'CG_TT
Cuts at: 1563 5325 5698 1563
Size: 3762 373 3063
Fragments arranged by size:
3762 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2581 2856 3708 3906 4106 5261 6645 6913
Size: 949 275 852 198 200 1155 1384 268
Cuts at: 6913 1632
Size: 1917
Fragments arranged by size:
1917 1384 1155 949 852 275 268 200
198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7001
AflIII A'CryG_T
Cuts at: 1203 3684 6097 7074 1203
Size: 2481 2413 977 1327
Fragments arranged by size:
2481 2413 1327 977
AgeI A'CCGG_T
Cuts at: 6029 6029
Size: 7198
AhdI GACnn_n'nnGTC
Cuts at: 5927 5927
Size: 7198
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4413 1184
Size: 3229 3969
AlwNI CAG_nnn'CTG
Cuts at: 2083 3418 3731 3884 3931 4941 6513 2083
Size: 1335 313 153 47 1010 1572 2768
Fragments arranged by size:
2768 1572 1335 1010 313 153 47
ApaLI G'TGCA_C
Cuts at: 5255 6411 5255
Size: 1156 6042
ApoI r'AATT_y
Cuts at: 1493 2510 2620 2680 2734 4155 4255 4267 4507
Size: 1017 110 60 54 1421 100 12 240
Cuts at: 4507 4733 4885 6994 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 1017 240 226 152 110
100 60 54 12
AseI AT'TA_AT
Cuts at: 160 1032 5752 160
Size: 872 4720 1606
Fragments arranged by size:
4720 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
AvaI C'yCGr_G
Cuts at: 1870 1943 4523 1870
Size: 73 2580 4545
Fragments arranged by size:
4545 2580 73
BamHI G'GATC_C
Cuts at: 2982 4528 4895 2982
Size: 1546 367 5285
Fragments arranged by size:
5285 1546 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4146 4519 5974 618
Size: 325 217 627 2359 373 1455 1842
Fragments arranged by size:
2359 1842 1455 627 373 325 217
BanII G_rGCy'C
Cuts at: 1391 2854 4517 1391
Size: 1463 1663 4072
Fragments arranged by size:
4072 1663 1463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6227
BbvCI CC'TCA_GC
Cuts at: 2099 2099
Size: 7198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5389 5423 1903
Size: 34 3452 34 3678
Fragments arranged by size:
3678 3452 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 2962 5090 6300 2350
Size: 612 2128 1210 3248
Fragments arranged by size:
3248 2128 1210 612
BclI T'GATC_A
Cuts at: 1548 3288 1548
Size: 1740 5458
BfrBI ATG'CAT
Cuts at: 3094 3094
Size: 7198
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2291 5809 136
Size: 107 122 71 631 605 619 3518 1525
Fragments arranged by size:
3518 1525 631 619 605 122 107 71
BglII A'GATC_T
Cuts at: 2479 2635 7193 2479
Size: 156 4558 2484
Fragments arranged by size:
4558 2484 156
BlpI GC'TnA_GC
Cuts at: 4615 4615
Size: 7198
Bme1580I G_kGCm'C
Cuts at: 1790 5259 6415 1790
Size: 3469 1156 2573
Fragments arranged by size:
3469 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 3126 4929
Size: 416 53 83 399 24 959 750 1803
Cuts at: 4929 5887 442
Size: 958 1753
Fragments arranged by size:
1803 1753 959 958 750 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 3129 3615 3753 4034 4121 5858 1239
Size: 1353 537 486 138 281 87 1737 2579
Fragments arranged by size:
2579 1737 1353 537 486 281 138 87
Bpu10I CC'TnA_GC
Cuts at: 2099 2099
Size: 7198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5323 6188 6486 5323
Size: 865 298 6035
Fragments arranged by size:
6035 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3802 5861 882
Size: 617 2303 2059 2219
Fragments arranged by size:
2303 2219 2059 617
BsaAI yAC'GTr
Cuts at: 493 1204 4989 493
Size: 711 3785 2702
Fragments arranged by size:
3785 2702 711
BsaBI GATnn'nnATC
Cuts at: 7192 7192
Size: 7198
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2230 3240 5005 5387
Size: 53 83 186 936 697 1010 1765 382
Cuts at: 5387 6022 275
Size: 635 1451
Fragments arranged by size:
1765 1451 1010 936 697 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4149 4243 5631 6029 6303 6450 6861 4149
Size: 94 1388 398 274 147 411 4486
Fragments arranged by size:
4486 1388 411 398 274 147 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3212 3242 3392 3422 4427
Size: 30 165 30 1809 30 150 30 1005
Cuts at: 4427 4457 1178
Size: 30 3919
Fragments arranged by size:
3919 1809 1005 165 150 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2057 2060 2270 1186
Size: 868 3 3 210 6114
Fragments arranged by size:
6114 868 210 3 3
BseYI C'CCAG_C
Cuts at: 2175 3024 6401 6897 2175
Size: 849 3377 496 2476
Fragments arranged by size:
3377 2476 849 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3397 1163
Size: 573 1661 4964
Fragments arranged by size:
4964 1661 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 2224 2266 4648 5409 6437
Size: 275 186 192 31 42 2382 761 1028
Cuts at: 6437 1540
Size: 2301
Fragments arranged by size:
2382 2301 1028 761 275 192 186 42
31
BsiHKAI G_wGCw'C
Cuts at: 3261 3621 4332 5259 5344 6415 3261
Size: 360 711 927 85 1071 4044
Fragments arranged by size:
4044 1071 927 711 360 85
BsmI GAATG_Cn'
Cuts at: 3457 4709 4802 3457
Size: 1252 93 5853
Fragments arranged by size:
5853 1252 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3200 3200
Size: 7198
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2854 3261 3621 4332 4517 5259 5344
Size: 399 1064 407 360 711 185 742 85
Cuts at: 5344 6415 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1064 742 711 407 399 360
185 85
BspEI T'CCGG_A
Cuts at: 4243 6861 4243
Size: 2618 4580
BspHI T'CATG_A
Cuts at: 4468 5087 6817 4468
Size: 619 1730 4849
Fragments arranged by size:
4849 1730 619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4553 844
Size: 3709 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 5085 1394
Size: 429 188 3074 3507
Fragments arranged by size:
3507 3074 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 3468 5693 5867 65
Size: 1183 2220 2225 174 1396
Fragments arranged by size:
2225 2220 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 5842 6029 1700
Size: 110 299 3733 187 2869
Fragments arranged by size:
3733 2869 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 3519 96
Size: 3423 3775
BssHII G'CGCG_C
Cuts at: 1925 2238 1925
Size: 313 6885
BssSI C'ACGA_G
Cuts at: 5258 6270 5258
Size: 1012 6186
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 794
Size: 1289 5909
BstBI TT'CG_AA
Cuts at: 6002 6002
Size: 7198
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7198
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3865 4069 4414 3172
Size: 693 204 345 5956
Fragments arranged by size:
5956 693 345 204
BstYI r'GATC_y
Cuts at: 1972 2479 2635 2982 4528 4895 5280 5297 6749
Size: 507 156 347 1546 367 385 17 1452
Cuts at: 6749 6835 6847 7193 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1546 1452 507 385 367 347 346
156 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3323 3323
Size: 7198
BtgI C'CryG_G
Cuts at: 513 1065 2004 2050 2215 3036 3235 513
Size: 552 939 46 165 821 199 4476
Fragments arranged by size:
4476 939 821 552 199 165 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6459
Fragments arranged by size:
6459 683 56
BtsI GCAGTG_nn'
Cuts at: 4714 5508 5528 4714
Size: 794 20 6384
Fragments arranged by size:
6384 794 20
ClaI AT'CG_AT
Cuts at: 1554 4375 1554
Size: 2821 4377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7163
DraI TTT'AAA
Cuts at: 3817 4502 3817
Size: 685 6513
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6205 809
Size: 507 4889 1802
Fragments arranged by size:
4889 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2221 2263
Size: 54 1425 50 275 18 360 31 42
Cuts at: 2263 2292 2778 3609 4287 4645 5534 7163 8
Size: 29 486 831 678 358 889 1629 43
Fragments arranged by size:
1629 1425 889 831 678 486 360 358
275 54 50 43 42 31 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2190 2221 2263 4645 1537
Size: 275 378 31 42 2382 4090
Fragments arranged by size:
4090 2382 378 275 42 31
EarI CTCTTCn'nnn_
Cuts at: 1353 3837 4114 5128 1353
Size: 2484 277 1014 3423
Fragments arranged by size:
3423 2484 1014 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3216 5785 6159 6305 387
Size: 1064 138 1627 2569 374 146 1280
Fragments arranged by size:
2569 1627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2581 2592 2856 3129 3615 3708 3753
Size: 393 949 11 264 273 486 93 45
Cuts at: 3753 3906 4034 4106 4121 5261 5858 6645 6913
Size: 153 128 72 15 1140 597 787 268
Cuts at: 6913 1239
Size: 1524
Fragments arranged by size:
1524 1140 949 787 597 486 393 273
268 264 153 128 93 72 45 15
11
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 7198
EcoO109I rG'GnC_Cy
Cuts at: 3566 3697 4486 3566
Size: 131 789 6278
Fragments arranged by size:
6278 789 131
EcoRI G'AATT_C
Cuts at: 2680 4255 4507 4885 2680
Size: 1575 252 378 4993
Fragments arranged by size:
4993 1575 378 252
EcoRV GAT'ATC
Cuts at: 3823 3823
Size: 7198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2861 3842 2861
Size: 981 6217
FspI TGC'GCA
Cuts at: 5704 5704
Size: 7198
HaeII r_GCGC'y
Cuts at: 1678 6345 1678
Size: 4667 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2577 2609 3141
Size: 32 135 32 449 32 524 32 532
Cuts at: 3141 3173 3482 3514 4427 4459 5839 5871 5913
Size: 32 309 32 913 32 1380 32 42
Cuts at: 5913 5945 1373
Size: 32 2626
Fragments arranged by size:
2626 1380 913 532 524 449 309 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2094 2556 4542 4788 669
Size: 988 281 156 462 1986 246 3079
Fragments arranged by size:
3079 1986 988 462 281 246 156
HindIII A'AGCT_T
Cuts at: 748 2887 748
Size: 2139 5059
HpaI GTT'AAC
Cuts at: 2556 4788 2556
Size: 2232 4966
KpnI G_GTAC'C
Cuts at: 4150 4523 4150
Size: 373 6825
MfeI C'AATT_G
Cuts at: 4797 4797
Size: 7198
MluI A'CGCG_T
Cuts at: 7074 7074
Size: 7198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2335 6312 6496 1305
Size: 1030 3977 184 2007
Fragments arranged by size:
3977 2007 1030 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2780 3611 4289 10
Size: 54 1425 343 462 486 831 678 2919
Fragments arranged by size:
2919 1425 831 678 486 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2310 2660 3099 3390 3500 3605
Size: 648 1002 142 350 439 291 110 105
Cuts at: 3605 3952 5156 5515 5674 518
Size: 347 1204 359 159 2042
Fragments arranged by size:
2042 1204 1002 648 439 359 350 347
291 159 142 110 105
MspA1I CmG'CkG
Cuts at: 1606 2080 2135 2143 2217 3237 4614 5291 6439
Size: 474 55 8 74 1020 1377 677 1148
Cuts at: 6439 6684 1606
Size: 245 2120
Fragments arranged by size:
2120 1377 1148 1020 677 474 245 74
55 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 2050 513
Size: 552 939 46 5661
Fragments arranged by size:
5661 939 552 46
NdeI CA'TA_TG
Cuts at: 387 3860 387
Size: 3473 3725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7198
NotI GC'GGCC_GC
Cuts at: 2190 4645 2190
Size: 2455 4743
NsiI A_TGCA'T
Cuts at: 3096 3096
Size: 7198
NspI r_CATG'y
Cuts at: 1132 1229 2315 3098 4400 4556 6101 1132
Size: 97 1086 783 1302 156 1545 2229
Fragments arranged by size:
2229 1545 1302 1086 783 156 97
PasI CC'CwG_GG
Cuts at: 1348 2016 1348
Size: 668 6530
PciI A'CATG_T
Cuts at: 6097 6097
Size: 7198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3792 4099 1108
Size: 2684 307 4207
Fragments arranged by size:
4207 2684 307
PfoI T'CCnGG_A
Cuts at: 2271 3545 3585 2271
Size: 1274 40 5884
Fragments arranged by size:
5884 1274 40
PmeI GTTT'AAAC
Cuts at: 4502 4502
Size: 7198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5267 5299 6780 6812 5267
Size: 32 1481 32 5653
Fragments arranged by size:
5653 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3566 3697 4486 3566
Size: 131 789 6278
Fragments arranged by size:
6278 789 131
PshAI GACnn'nnGTC
Cuts at: 1613 3663 1613
Size: 2050 5148
PsiI TTA'TAA
Cuts at: 4768 4768
Size: 7198
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4176 4208 4176
Size: 32 7166
PstI C_TGCA'G
Cuts at: 830 2079 4392 4550 830
Size: 1249 2313 158 3478
Fragments arranged by size:
3478 2313 1249 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7198
PvuII CAG'CTG
Cuts at: 2080 2080
Size: 7198
SacII CC_GC'GG
Cuts at: 2218 3238 2218
Size: 1020 6178
SalI G'TCGA_C
Cuts at: 1936 2092 4540 1936
Size: 156 2448 4594
Fragments arranged by size:
4594 2448 156
SanDI GG'GwC_CC
Cuts at: 3697 3697
Size: 7198
SbfI CC_TGCA'GG
Cuts at: 4550 4550
Size: 7198
ScaI AGT'ACT
Cuts at: 5446 7158 5446
Size: 1712 5486
SfcI C'TryA_G
Cuts at: 826 1045 2075 3354 3400 4388 4546 5681 6362
Size: 219 1030 1279 46 988 158 1135 681
Cuts at: 6362 6553 826
Size: 191 1471
Fragments arranged by size:
1471 1279 1135 1030 988 681 219 191
158 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7198
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7198
SmaI CCC'GGG
Cuts at: 1872 4525 1872
Size: 2653 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5302 6203 6465 820
Size: 197 926 3359 901 262 1553
Fragments arranged by size:
3359 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4989 493
Size: 4496 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7198
SphI G_CATG'C
Cuts at: 1132 4400 4556 1132
Size: 3268 156 3774
Fragments arranged by size:
3774 3268 156
SspI AAT'ATT
Cuts at: 5 52 5122 5
Size: 47 5070 2081
Fragments arranged by size:
5070 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7198
StyI C'CwwG_G
Cuts at: 513 1065 2004 2050 2845 3301 4637 513
Size: 552 939 46 795 456 1336 3074
Fragments arranged by size:
3074 1336 939 795 552 456 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2713 3192 5225 5242 5395 5580 6025
Size: 1255 1133 479 2033 17 153 185 445
Cuts at: 6025 325
Size: 1498
Fragments arranged by size:
2033 1498 1255 1133 479 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3519 5444 7156
Size: 274 80 33 51 1443 1542 1925 1712
Cuts at: 7156 96
Size: 138
Fragments arranged by size:
1925 1712 1542 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2535 2567 7158 7190 2243
Size: 32 260 32 4591 32 2251
Fragments arranged by size:
4591 2251 260 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2279 1316
Size: 963 6235
XbaI T'CTAG_A
Cuts at: 4534 4534
Size: 7198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3502 3502
Size: 7198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7198
XmaI C'CCGG_G
Cuts at: 1870 4523 1870
Size: 2653 4545
XmnI GAAnn'nnTTC
Cuts at: 3927 5327 3927
Size: 1400 5798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5006 276
Size: 53 83 186 936 3472 2468
Fragments arranged by size:
3472 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI