Restriction map for
pFN21AB5788
(Circular) (Six-base) MAPSORT of: pFN21AB5788.seq Check: 6577 from: 1 to: 5761
pFN21AB5788 5761 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 2997 3104 1937
Size: 727 333 107 4594
Fragments arranged by size:
4594 727 333 107
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2160 2609 2648 3824 5208 5476 1632
Size: 528 449 39 1176 1384 268 1917
Fragments arranged by size:
1917 1384 1176 528 449 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2943 4660 5637 1203
Size: 1740 1717 977 1327
Fragments arranged by size:
1740 1717 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 3031 4490 3031
Size: 1459 4302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 3504 5076 3504
Size: 1572 4189
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2490 3086 1870
Size: 73 547 596 4545
Fragments arranged by size:
4545 596 547 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3082 4537 618
Size: 325 217 627 1295 1455 1842
Fragments arranged by size:
1842 1455 1295 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2499 3080 1391
Size: 625 483 581 4072
Fragments arranged by size:
4072 625 581 483
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2532 928
Size: 971 282 351 4157
Fragments arranged by size:
4157 971 351 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3017 3051 3952 3986 1903
Size: 34 1080 34 901 34 3678
Fragments arranged by size:
3678 1080 901 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2261 2319 3044
Size: 107 122 71 631 605 589 58 725
Cuts at: 3044 4372 136
Size: 1328 1525
Fragments arranged by size:
1525 1328 725 631 605 589 122 107
71 58
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2010 3178 2010
Size: 1168 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2577 3822 4978 1790
Size: 787 1245 1156 2573
Fragments arranged by size:
2573 1245 1156 787
BmgBI CAC'GTC
Cuts at: 1318 2824 2946 1318
Size: 1506 122 4133
Fragments arranged by size:
4133 1506 122
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3023 1056
Size: 1967 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 4421 1239
Size: 1053 2129 2579
Fragments arranged by size:
2579 2129 1053
Bpu10I CC'TnA_GC
Cuts at: 2681 2681
Size: 5761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2194 3552 493
Size: 711 990 1358 2702
Fragments arranged by size:
2702 1358 990 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 1178
Size: 30 165 30 1497 30 4009
Fragments arranged by size:
4009 1497 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2962 1186
Size: 1776 3985
BseYI C'CCAG_C
Cuts at: 2048 2165 2378 3006 4964 5460 2048
Size: 117 213 628 1958 496 2349
Fragments arranged by size:
2349 1958 628 496 213 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3822 3907 4978 3822
Size: 85 1071 4605
Fragments arranged by size:
4605 1071 85
BsiWI C'GTAC_G
Cuts at: 2055 2055
Size: 5761
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
BsmBI CGTCTCn'nnnn_
Cuts at: 2952 2952
Size: 5761
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2499 2577 3080 3822 3907 4978
Size: 399 226 483 78 503 742 85 1071
Cuts at: 4978 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 503 483 399 226 85
78
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2363 2457 3648 1394
Size: 429 540 94 1191 3507
Fragments arranged by size:
3507 1191 540 429 94
BsrDI GCAATG_nn'
Cuts at: 65 1248 2971 4256 4430 65
Size: 1183 1723 1285 174 1396
Fragments arranged by size:
1723 1396 1285 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2978 4405 4592 1700
Size: 110 1168 1427 187 2869
Fragments arranged by size:
2869 1427 1168 187 110
BsrGI T'GTAC_A
Cuts at: 96 2916 96
Size: 2820 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 2028 4565 2028
Size: 2537 3224
BstEII G'GTnAC_C
Cuts at: 1184 2689 1184
Size: 1505 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2828 2506
Size: 322 5439
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2291 2899 2951 513
Size: 552 939 287 608 52 3323
Fragments arranged by size:
3323 939 608 552 287 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2256 487
Size: 683 56 1030 3992
Fragments arranged by size:
3992 1030 683 56
BtsI GCAGTG_nn'
Cuts at: 2517 3277 4071 4091 2517
Size: 760 794 20 4187
Fragments arranged by size:
4187 794 760 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3045 3208
Size: 54 1425 50 275 18 910 305 163
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 305 275 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2533 2847 2921 4348 4722 4868
Size: 1064 138 944 314 74 1427 374 146
Cuts at: 4868 387
Size: 1280
Fragments arranged by size:
1427 1280 1064 944 374 314 146 138
74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2292 2609 2648 3824 4421 5208
Size: 393 528 132 317 39 1176 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 1176 787 597 528 393 317 268
132 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2832 2684
Size: 148 5613
EcoO109I rG'GnC_Cy
Cuts at: 2802 2802
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2884 2788
Size: 96 5665
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 2188 2220 3017
Size: 32 135 32 454 32 130 32 797
Cuts at: 3017 3049 4402 4434 4476 4508 1373
Size: 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 797 454 135 130 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3105 3351 669
Size: 988 281 739 428 246 3079
Fragments arranged by size:
3079 988 739 428 281 246
HindIII A'AGCT_T
Cuts at: 748 2765 748
Size: 2017 3744
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2501 4875 5059 1305
Size: 1196 2374 184 2007
Fragments arranged by size:
2374 2007 1196 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2742 3047 10
Size: 54 1425 343 910 305 2724
Fragments arranged by size:
2724 1425 910 343 305 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2990 3719 4078 4237 518
Size: 648 1824 729 359 159 2042
Fragments arranged by size:
2042 1824 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2415 3177 3854 5002 5247 1606
Size: 687 122 762 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 687 677 245 122
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 3019 1052
Size: 1967 3794
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2738 1108
Size: 1630 4131
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2194 2194
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2802 2802
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2562 3113 830
Size: 1732 551 3478
Fragments arranged by size:
3478 1732 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
SacII CC_GC'GG
Cuts at: 2294 2294
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2802 2802
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2419 2558 3109 4244 4925 5116 826
Size: 219 1374 139 551 1135 681 191 1471
Fragments arranged by size:
1471 1374 1135 681 551 219 191 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 2319 2319
Size: 5761
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2492 3088 1872
Size: 620 596 4545
Fragments arranged by size:
4545 620 596
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 3200 513
Size: 552 939 252 944 3074
Fragments arranged by size:
3074 944 939 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2392 2500 2542 2834 3788 3805 3958
Size: 1255 812 108 42 292 954 17 153
Cuts at: 3958 4143 4588 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 954 812 445 292 185 153
108 42 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 4007 5719
Size: 274 80 33 51 1443 939 1091 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1091 939 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 5721 5753 2326
Size: 32 3363 32 2334
Fragments arranged by size:
3363 2334 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 1316
Size: 1467 4294
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2795 3055 2795
Size: 260 5501
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2490 3086 1870
Size: 620 596 4545
Fragments arranged by size:
4545 620 596
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI
SapI SexAI SfoI SrfI SwaI