Restriction map for
pFN21AB5787
(Circular) (Six-base) MAPSORT of: pFN21AB5787.seq Check: 7461 from: 1 to: 7192
pFN21AB5787 7192 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2701 2701
Size: 7192
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5002 278
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3387 4535 1937
Size: 1450 1148 4594
Fragments arranged by size:
4594 1450 1148
Acc65I G'GTAC_C
Cuts at: 4513 4513
Size: 7192
AclI AA'CG_TT
Cuts at: 1563 5319 5692 1563
Size: 3756 373 3063
Fragments arranged by size:
3756 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 2127 2285 3278 3842 3854 4310 5255
Size: 448 47 158 993 564 12 456 945
Cuts at: 5255 6639 6907 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 993 945 564 456 448 268
158 47 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6995
AflIII A'CryG_T
Cuts at: 1203 4467 6091 7068 1203
Size: 3264 1624 977 1327
Fragments arranged by size:
3264 1624 1327 977
AgeI A'CCGG_T
Cuts at: 6023 6023
Size: 7192
AhdI GACnn_n'nnGTC
Cuts at: 4372 5921 4372
Size: 1549 5643
AleI CACnn'nnGTG
Cuts at: 1166 2939 3085 3284 3494 1166
Size: 1773 146 199 210 4864
Fragments arranged by size:
4864 1773 210 199 146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7192
AlwNI CAG_nnn'CTG
Cuts at: 2155 2386 3049 3647 3697 4935 6507 2155
Size: 231 663 598 50 1238 1572 2840
Fragments arranged by size:
2840 1572 1238 663 598 231 50
ApaLI G'TGCA_C
Cuts at: 3854 5249 6405 3854
Size: 1395 1156 4641
Fragments arranged by size:
4641 1395 1156
ApoI r'AATT_y
Cuts at: 1493 4501 4727 4879 6988 1493
Size: 3008 226 152 2109 1697
Fragments arranged by size:
3008 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5746 160
Size: 872 4714 1606
Fragments arranged by size:
4714 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
AvaI C'yCGr_G
Cuts at: 1870 1943 4517 1870
Size: 73 2574 4545
Fragments arranged by size:
4545 2574 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2688 2721 4217 4250 2688
Size: 33 1496 33 5630
Fragments arranged by size:
5630 1496 33 33
BamHI G'GATC_C
Cuts at: 4522 4889 4522
Size: 367 6825
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 2834 3313 3500 4207
Size: 325 217 627 792 255 479 187 707
Cuts at: 4207 4513 5968 618
Size: 306 1455 1842
Fragments arranged by size:
1842 1455 792 707 627 479 325 306
255 217 187
BanII G_rGCy'C
Cuts at: 1391 2013 2145 2553 3654 4062 4385 4511 1391
Size: 622 132 408 1101 408 323 126 4072
Fragments arranged by size:
4072 1101 622 408 408 323 132 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 2320 2467 3405 4090 928
Size: 971 187 234 147 938 685 4030
Fragments arranged by size:
4030 971 938 685 234 187 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5383 5417 1903
Size: 34 3446 34 3678
Fragments arranged by size:
3678 3446 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3835 5084 6294 3835
Size: 1249 1210 4733
Fragments arranged by size:
4733 1249 1210
BclI T'GATC_A
Cuts at: 1548 3900 1548
Size: 2352 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5803 136
Size: 107 122 71 631 605 4131 1525
Fragments arranged by size:
4131 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2286 7187 2286
Size: 4901 2291
BlpI GC'TnA_GC
Cuts at: 4609 4609
Size: 7192
Bme1580I G_kGCm'C
Cuts at: 1790 3503 3858 5253 6409 1790
Size: 1713 355 1395 1156 2573
Fragments arranged by size:
2573 1713 1395 1156 355
BmgBI CAC'GTC
Cuts at: 1318 3853 4204 1318
Size: 2535 351 4306
Fragments arranged by size:
4306 2535 351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 3635 4923
Size: 416 53 83 399 24 1961 257 1288
Cuts at: 4923 5881 442
Size: 958 1753
Fragments arranged by size:
1961 1753 1288 958 416 399 257 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3464 1056
Size: 2408 4784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2836 4190 5852 1239
Size: 791 806 1354 1662 2579
Fragments arranged by size:
2579 1662 1354 806 791
Bpu10I CC'TnA_GC
Cuts at: 3648 4379 3648
Size: 731 6461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 5317 6182 6480 2381
Size: 2936 865 298 3093
Fragments arranged by size:
3093 2936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3438 5855 882
Size: 617 1939 2417 2219
Fragments arranged by size:
2417 2219 1939 617
BsaAI yAC'GTr
Cuts at: 493 1204 3295 4983 493
Size: 711 2091 1688 2702
Fragments arranged by size:
2702 2091 1688 711
BsaBI GATnn'nnATC
Cuts at: 3665 7186 3665
Size: 3521 3671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3069 4368 4999 5381
Size: 53 83 186 936 1536 1299 631 382
Cuts at: 5381 6016 275
Size: 635 1451
Fragments arranged by size:
1536 1451 1299 936 635 631 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2823 5625 6023 6297 6444 6855 2823
Size: 2802 398 274 147 411 3160
Fragments arranged by size:
3160 2802 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3365 3395 1178
Size: 30 165 30 1962 30 4975
Fragments arranged by size:
4975 1962 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2546 4384 1186
Size: 841 519 1838 3994
Fragments arranged by size:
3994 1838 841 519
BseYI C'CCAG_C
Cuts at: 2155 3448 3747 3982 4152 6395 6891 2155
Size: 1293 299 235 170 2243 496 2456
Fragments arranged by size:
2456 2243 1293 496 299 235 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3062 1163
Size: 573 1326 5293
Fragments arranged by size:
5293 1326 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4642 5403 6431 1540
Size: 275 186 1510 1131 761 1028 2301
Fragments arranged by size:
2301 1510 1131 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2831 3858 4385 5253 5338 6409 2553
Size: 278 1027 527 868 85 1071 3336
Fragments arranged by size:
3336 1071 1027 868 527 278 85
BsmI GAATG_Cn'
Cuts at: 3028 4703 4796 3028
Size: 1675 93 5424
Fragments arranged by size:
5424 1675 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2037 2037
Size: 7192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2145 2553 2831 3503 3654 3858
Size: 399 223 132 408 278 672 151 204
Cuts at: 3858 4062 4385 4511 5253 5338 6409 1391
Size: 204 323 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 408 399 323 278
223 204 204 151 132 126 85
BspEI T'CCGG_A
Cuts at: 6855 6855
Size: 7192
BspHI T'CATG_A
Cuts at: 5081 6811 5081
Size: 1730 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2701 3475 3950 4547 844
Size: 1857 774 475 597 3489
Fragments arranged by size:
3489 1857 774 597 475
BsrBI CCG'CTC
Cuts at: 1394 1823 5079 1394
Size: 429 3256 3507
Fragments arranged by size:
3507 3256 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4204 5687 5861 65
Size: 1183 2956 1483 174 1396
Fragments arranged by size:
2956 1483 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5836 6023 1700
Size: 110 4026 187 2869
Fragments arranged by size:
4026 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7192
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7192
BssSI C'ACGA_G
Cuts at: 2016 5252 6264 2016
Size: 3236 1012 2944
Fragments arranged by size:
3236 2944 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7192
BstBI TT'CG_AA
Cuts at: 5996 5996
Size: 7192
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7192
BstXI CCAn_nnnn'nTGG
Cuts at: 2860 2911 2941 3286 3456 2860
Size: 51 30 345 170 6596
Fragments arranged by size:
6596 345 170 51 30
BstYI r'GATC_y
Cuts at: 1972 2286 2955 4522 4889 5274 5291 6743 6829
Size: 314 669 1567 367 385 17 1452 86
Cuts at: 6829 6841 7187 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1567 1452 669 385 367 346 314
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2466 2466
Size: 7192
BtgI C'CryG_G
Cuts at: 513 1065 2004 2721 2934 3465 3564 3634 3874
Size: 552 939 717 213 531 99 70 240
Cuts at: 3874 3925 513
Size: 51 3780
Fragments arranged by size:
3780 939 717 552 531 240 213 99
70 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6453
Fragments arranged by size:
6453 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2946 3033 3390 4708 5502 5522 2069
Size: 877 87 357 1318 794 20 3739
Fragments arranged by size:
3739 1318 877 794 357 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7157
DraI TTT'AAA
Cuts at: 4496 4496
Size: 7192
DraIII CAC_nnn'GTG
Cuts at: 2566 3915 2566
Size: 1349 5843
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3809 6199 809
Size: 507 2493 2390 1802
Fragments arranged by size:
2493 2390 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 2557 3561
Size: 54 1425 50 275 18 316 411 1004
Cuts at: 3561 3928 4244 4639 5528 7157 8
Size: 367 316 395 889 1629 43
Fragments arranged by size:
1629 1425 1004 889 411 395 367 316
316 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4639 1537
Size: 275 2827 4090
Fragments arranged by size:
4090 2827 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4107 4122 5122 1353
Size: 2754 15 1000 3423
Fragments arranged by size:
3423 2754 1000 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 5779 6153 6299 387
Size: 1064 138 2798 1392 374 146 1280
Fragments arranged by size:
2798 1392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 4383 2551
Size: 1832 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2080 2127 2285 2836 3278 3842
Size: 393 398 50 47 158 551 442 564
Cuts at: 3842 3854 4190 4310 5255 5852 6639 6907 1239
Size: 12 336 120 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 564 551 442 398
393 336 268 158 120 50 47 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 3836 2045
Size: 1791 5401
EcoO109I rG'GnC_Cy
Cuts at: 2369 2593 2794 3244 3715 2369
Size: 224 201 450 471 5846
Fragments arranged by size:
5846 471 450 224 201
EcoRI G'AATT_C
Cuts at: 4501 4879 4501
Size: 378 6814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 7192
FspI TGC'GCA
Cuts at: 5698 5698
Size: 7192
HaeII r_GCGC'y
Cuts at: 1678 2156 6339 1678
Size: 478 4183 2531
Fragments arranged by size:
4183 2531 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 2658 2690 3954
Size: 32 135 32 802 32 252 32 1264
Cuts at: 3954 3986 4358 4390 5833 5865 5907 5939 1373
Size: 32 372 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 1264 802 372 252 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3790 4536 4782 669
Size: 988 281 1852 746 246 3079
Fragments arranged by size:
3079 1852 988 746 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7192
HpaI GTT'AAC
Cuts at: 4782 4782
Size: 7192
KpnI G_GTAC'C
Cuts at: 4517 4517
Size: 7192
MfeI C'AATT_G
Cuts at: 4791 4791
Size: 7192
MluI A'CGCG_T
Cuts at: 7068 7068
Size: 7192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4389 4428 6306 6490 1305
Size: 3084 39 1878 184 2007
Fragments arranged by size:
3084 2007 1878 184 39
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2559 3563 3930 10
Size: 54 1425 343 727 1004 367 3272
Fragments arranged by size:
3272 1425 1004 727 367 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 2909 2939 3085 3284 3494 3930
Size: 648 1449 294 30 146 199 210 436
Cuts at: 3930 5150 5509 5668 518
Size: 1220 359 159 2042
Fragments arranged by size:
2042 1449 1220 648 436 359 294 210
199 159 146 30
MspA1I CmG'CkG
Cuts at: 1606 3494 3892 4359 4608 5285 6433 6678 1606
Size: 1888 398 467 249 677 1148 245 2120
Fragments arranged by size:
2120 1888 1148 677 467 398 249 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3465 3564 3634 3874 3925 513
Size: 552 939 1461 99 70 240 51 3780
Fragments arranged by size:
3780 1461 939 552 240 99 70 51
NdeI CA'TA_TG
Cuts at: 387 3847 387
Size: 3460 3732
NheI G'CTAG_C
Cuts at: 1052 3460 1052
Size: 2408 4784
NotI GC'GGCC_GC
Cuts at: 4639 4639
Size: 7192
NspI r_CATG'y
Cuts at: 1132 1229 4471 4550 6095 1132
Size: 97 3242 79 1545 2229
Fragments arranged by size:
3242 2229 1545 97 79
PasI CC'CwG_GG
Cuts at: 1348 2180 1348
Size: 832 6360
PciI A'CATG_T
Cuts at: 4467 6091 4467
Size: 1624 5568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 3479 1108
Size: 1047 1324 4821
Fragments arranged by size:
4821 1324 1047
PfoI T'CCnGG_A
Cuts at: 2247 3351 2247
Size: 1104 6088
PmeI GTTT'AAAC
Cuts at: 4496 4496
Size: 7192
PmlI CAC'GTG
Cuts at: 3295 3295
Size: 7192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5261 5293 6774 6806 5261
Size: 32 1481 32 5647
Fragments arranged by size:
5647 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2369 2593 2794 3715 2369
Size: 224 201 921 5846
Fragments arranged by size:
5846 921 224 201
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7192
PsiI TTA'TAA
Cuts at: 4277 4762 4277
Size: 485 6707
PstI C_TGCA'G
Cuts at: 830 2064 3397 4544 830
Size: 1234 1333 1147 3478
Fragments arranged by size:
3478 1333 1234 1147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7192
PvuII CAG'CTG
Cuts at: 3494 3892 3494
Size: 398 6794
SacI G_AGCT'C
Cuts at: 2553 4385 2553
Size: 1832 5360
SalI G'TCGA_C
Cuts at: 1936 4534 1936
Size: 2598 4594
SanDI GG'GwC_CC
Cuts at: 2369 2369
Size: 7192
SbfI CC_TGCA'GG
Cuts at: 4544 4544
Size: 7192
ScaI AGT'ACT
Cuts at: 5440 7152 5440
Size: 1712 5480
SexAI A'CCwGG_T
Cuts at: 2692 2692
Size: 7192
SfcI C'TryA_G
Cuts at: 826 1045 2060 2431 2481 3393 4540 5675 6356
Size: 219 1015 371 50 912 1147 1135 681
Cuts at: 6356 6547 826
Size: 191 1471
Fragments arranged by size:
1471 1147 1135 1015 912 681 371 219
191 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7192
SmaI CCC'GGG
Cuts at: 1872 4519 1872
Size: 2647 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 5296 6197 6459 820
Size: 197 926 453 2900 901 262 1553
Fragments arranged by size:
2900 1553 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 4983 493
Size: 4490 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7192
SphI G_CATG'C
Cuts at: 1132 4550 1132
Size: 3418 3774
SspI AAT'ATT
Cuts at: 5 52 5116 5
Size: 47 5064 2081
Fragments arranged by size:
5064 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7192
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 3465 3564 3634 3874 3925
Size: 552 939 111 1350 99 70 240 51
Cuts at: 3925 4631 513
Size: 706 3074
Fragments arranged by size:
3074 1350 939 706 552 240 111 99
70 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2320 2636 3161 4166 4243 5219 5236
Size: 1255 740 316 525 1005 77 976 17
Cuts at: 5236 5389 5574 6019 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 976 740 525 445 316
185 153 77 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 5438 7150
Size: 274 80 33 51 1443 906 2555 1712
Cuts at: 7150 96
Size: 138
Fragments arranged by size:
2555 1712 1443 906 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2880 2912 3365 3397 7152 7184 2880
Size: 32 453 32 3755 32 2888
Fragments arranged by size:
3755 2888 453 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4094 1316
Size: 2778 4414
XbaI T'CTAG_A
Cuts at: 4528 4528
Size: 7192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2938 3187 4183 2938
Size: 249 996 5947
Fragments arranged by size:
5947 996 249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7192
XmaI C'CCGG_G
Cuts at: 1870 4517 1870
Size: 2647 4545
XmnI GAAnn'nnTTC
Cuts at: 2165 2288 3827 5321 2165
Size: 123 1539 1494 4036
Fragments arranged by size:
4036 1539 1494 123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5000 276
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SapI SfiI SfoI SrfI SwaI