Restriction map for
pFN21AB5786
(Circular) (Six-base) MAPSORT of: pFN21AB5786.seq Check: 3348 from: 1 to: 6892
pFN21AB5786 6892 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4702 278
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2293 2834 4235 1937
Size: 356 541 1401 4594
Fragments arranged by size:
4594 1401 541 356
Acc65I G'GTAC_C
Cuts at: 4213 4213
Size: 6892
AclI AA'CG_TT
Cuts at: 1563 2860 5019 5392 1563
Size: 1297 2159 373 3063
Fragments arranged by size:
3063 2159 1297 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2290 3093 4955 6339 6607 1632
Size: 658 803 1862 1384 268 1917
Fragments arranged by size:
1917 1862 1384 803 658 268
AfeI AGC'GCT
Cuts at: 4016 4016
Size: 6892
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6695
AflIII A'CryG_T
Cuts at: 1203 2058 2634 2829 5791 6768 1203
Size: 855 576 195 2962 977 1327
Fragments arranged by size:
2962 1327 977 855 576 195
AgeI A'CCGG_T
Cuts at: 5723 5723
Size: 6892
AhdI GACnn_n'nnGTC
Cuts at: 5621 5621
Size: 6892
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6892
AlwNI CAG_nnn'CTG
Cuts at: 2666 3500 4635 6207 2666
Size: 834 1135 1572 3351
Fragments arranged by size:
3351 1572 1135 834
ApaLI G'TGCA_C
Cuts at: 4949 6105 4949
Size: 1156 5736
ApoI r'AATT_y
Cuts at: 1493 2200 2477 2609 2817 3036 3351 4201 4427
Size: 707 277 132 208 219 315 850 226
Cuts at: 4427 4579 6688 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 850 707 315 277 226 219
208 152 132
AseI AT'TA_AT
Cuts at: 160 1032 5446 160
Size: 872 4414 1606
Fragments arranged by size:
4414 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
AvaI C'yCGr_G
Cuts at: 1870 1943 3711 3947 4217 1870
Size: 73 1768 236 270 4545
Fragments arranged by size:
4545 1768 270 236 73
BamHI G'GATC_C
Cuts at: 3943 4222 4589 3943
Size: 279 367 6246
Fragments arranged by size:
6246 367 279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4213 5668 618
Size: 325 217 627 2426 1455 1842
Fragments arranged by size:
2426 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 2673 4211 1391
Size: 725 557 1538 4072
Fragments arranged by size:
4072 1538 725 557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5083 5117 1903
Size: 34 3146 34 3678
Fragments arranged by size:
3678 3146 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3309 4784 5994 3309
Size: 1475 1210 4207
Fragments arranged by size:
4207 1475 1210
BclI T'GATC_A
Cuts at: 1548 3601 1548
Size: 2053 4839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5503 136
Size: 107 122 71 631 605 3831 1525
Fragments arranged by size:
3831 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2471 6887 2471
Size: 4416 2476
BlpI GC'TnA_GC
Cuts at: 3864 4309 3864
Size: 445 6447
Bme1580I G_kGCm'C
Cuts at: 1790 4050 4953 6109 1790
Size: 2260 903 1156 2573
Fragments arranged by size:
2573 2260 1156 903
BmgBI CAC'GTC
Cuts at: 1318 2061 1318
Size: 743 6149
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4623 5581 442
Size: 416 53 83 399 24 3206 958 1753
Fragments arranged by size:
3206 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3387 3597 3937 5552 1239
Size: 2148 210 340 1615 2579
Fragments arranged by size:
2579 2148 1615 340 210
Bpu10I CC'TnA_GC
Cuts at: 2107 2667 3159 2107
Size: 560 492 5840
Fragments arranged by size:
5840 560 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 5017 5882 6180 2525
Size: 2492 865 298 3237
Fragments arranged by size:
3237 2492 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2529 5555 882
Size: 617 1030 3026 2219
Fragments arranged by size:
3026 2219 1030 617
BsaAI yAC'GTr
Cuts at: 493 1204 4683 493
Size: 711 3479 2702
Fragments arranged by size:
3479 2702 711
BsaBI GATnn'nnATC
Cuts at: 6886 6886
Size: 6892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2077 3984 4699 5081
Size: 53 83 186 936 544 1907 715 382
Cuts at: 5081 5716 275
Size: 635 1451
Fragments arranged by size:
1907 1451 936 715 635 544 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4116 5325 5723 5997 6144 6555 4116
Size: 1209 398 274 147 411 4453
Fragments arranged by size:
4453 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 1178
Size: 30 165 30 1003 30 5634
Fragments arranged by size:
5634 1003 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3195 1186
Size: 2009 4883
BseYI C'CCAG_C
Cuts at: 3755 6095 6591 3755
Size: 2340 496 4056
Fragments arranged by size:
4056 2340 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2163 3239 3663 4085 1163
Size: 573 427 1076 424 422 3970
Fragments arranged by size:
3970 1076 573 427 424 422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4342 5103 6131 1540
Size: 275 186 2341 761 1028 2301
Fragments arranged by size:
2341 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2116 2673 4953 5038 6109 2116
Size: 557 2280 85 1071 2899
Fragments arranged by size:
2899 2280 1071 557 85
BsmI GAATG_Cn'
Cuts at: 3430 4403 4496 3430
Size: 973 93 5826
Fragments arranged by size:
5826 973 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2673 4050 4211 4953 5038 6109
Size: 399 326 557 1377 161 742 85 1071
Cuts at: 6109 1391
Size: 2174
Fragments arranged by size:
2174 1377 1071 742 557 399 326 161
85
BspEI T'CCGG_A
Cuts at: 6555 6555
Size: 6892
BspHI T'CATG_A
Cuts at: 2907 4781 6511 2907
Size: 1874 1730 3288
Fragments arranged by size:
3288 1874 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4247 844
Size: 3403 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2106 4779 1394
Size: 429 283 2673 3507
Fragments arranged by size:
3507 2673 429 283
BsrDI GCAATG_nn'
Cuts at: 65 1248 2021 2611 3859 5387 5561 65
Size: 1183 773 590 1248 1528 174 1396
Fragments arranged by size:
1528 1396 1248 1183 773 590 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5536 5723 1700
Size: 110 3726 187 2869
Fragments arranged by size:
3726 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6892
BssSI C'ACGA_G
Cuts at: 4061 4952 5964 4061
Size: 891 1012 4989
Fragments arranged by size:
4989 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6892
BstBI TT'CG_AA
Cuts at: 2361 5696 2361
Size: 3335 3557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6892
BstXI CCAn_nnnn'nTGG
Cuts at: 4043 4043
Size: 6892
BstYI r'GATC_y
Cuts at: 1972 2471 3943 4222 4589 4974 4991 6443 6529
Size: 499 1472 279 367 385 17 1452 86
Cuts at: 6529 6541 6887 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1472 1452 499 385 367 346 279
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3700 4036 513
Size: 552 2635 336 3369
Fragments arranged by size:
3369 2635 552 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6153
Fragments arranged by size:
6153 683 56
BtsI GCAGTG_nn'
Cuts at: 3181 3321 3382 3541 3678 4073 4408 5202 5222
Size: 140 61 159 137 395 335 794 20
Cuts at: 5222 3181
Size: 4851
Fragments arranged by size:
4851 794 395 335 159 140 137 61
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6892
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6857
DraI TTT'AAA
Cuts at: 2334 4196 2334
Size: 1862 5030
DraIII CAC_nnn'GTG
Cuts at: 3217 3565 3217
Size: 348 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5899 809
Size: 507 4583 1802
Fragments arranged by size:
4583 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 3703 4339
Size: 54 1425 50 275 18 1366 507 636
Cuts at: 4339 5228 6857 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1366 889 636 507 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4339 1537
Size: 275 2527 4090
Fragments arranged by size:
4090 2527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2309 2678 4031 4822 1353
Size: 956 369 1353 791 3423
Fragments arranged by size:
3423 1353 956 791 369
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3461 5479 5853 5999 387
Size: 1064 138 1872 2018 374 146 1280
Fragments arranged by size:
2018 1872 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2114 2671 2114
Size: 557 6335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 3093 3387 3597 3937 4955 5552
Size: 393 658 803 294 210 340 1018 597
Cuts at: 5552 6339 6607 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1018 803 787 658 597 393 340
294 268 210
EcoO109I rG'GnC_Cy
Cuts at: 2016 2016
Size: 6892
EcoRI G'AATT_C
Cuts at: 2817 4201 4579 2817
Size: 1384 378 5130
Fragments arranged by size:
5130 1384 378
EcoRV GAT'ATC
Cuts at: 4131 4131
Size: 6892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3065 3249 3065
Size: 184 6708
FspI TGC'GCA
Cuts at: 5398 5398
Size: 6892
HaeII r_GCGC'y
Cuts at: 1678 4018 6039 1678
Size: 2340 2021 2531
Fragments arranged by size:
2531 2340 2021
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2320 2352 2515 2547 3163
Size: 32 135 32 748 32 163 32 616
Cuts at: 3163 3195 3545 3577 5533 5565 5607 5639 1373
Size: 32 350 32 1956 32 42 32 2626
Fragments arranged by size:
2626 1956 748 616 350 163 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4236 4482 669
Size: 988 281 2298 246 3079
Fragments arranged by size:
3079 2298 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6892
HpaI GTT'AAC
Cuts at: 4482 4482
Size: 6892
KpnI G_GTAC'C
Cuts at: 4217 4217
Size: 6892
MfeI C'AATT_G
Cuts at: 4491 4491
Size: 6892
MluI A'CGCG_T
Cuts at: 6768 6768
Size: 6892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2309 3364 3960 6006 6190 1305
Size: 1004 1055 596 2046 184 2007
Fragments arranged by size:
2046 2007 1055 1004 596 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3198 10
Size: 54 1425 343 1366 3704
Fragments arranged by size:
3704 1425 1366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2070 4041 4850 5209 5368 518
Size: 648 904 1971 809 359 159 2042
Fragments arranged by size:
2042 1971 904 809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3551 4308 4985 6133 6378 1606
Size: 1945 757 677 1148 245 2120
Fragments arranged by size:
2120 1945 1148 757 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6340
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6892
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6892
NotI GC'GGCC_GC
Cuts at: 4339 4339
Size: 6892
NspI r_CATG'y
Cuts at: 1132 1229 2833 4250 5795 1132
Size: 97 1604 1417 1545 2229
Fragments arranged by size:
2229 1604 1545 1417 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6892
PciI A'CATG_T
Cuts at: 2829 5791 2829
Size: 2962 3930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6892
PfoI T'CCnGG_A
Cuts at: 3359 3359
Size: 6892
PmeI GTTT'AAAC
Cuts at: 4196 4196
Size: 6892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 6474 6506 4961
Size: 32 1481 32 5347
Fragments arranged by size:
5347 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2016
Size: 6892
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6892
PsiI TTA'TAA
Cuts at: 4462 4462
Size: 6892
PstI C_TGCA'G
Cuts at: 830 2500 3328 4244 830
Size: 1670 828 916 3478
Fragments arranged by size:
3478 1670 916 828
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6892
PvuII CAG'CTG
Cuts at: 3551 3551
Size: 6892
SacI G_AGCT'C
Cuts at: 2116 2673 2116
Size: 557 6335
SalI G'TCGA_C
Cuts at: 1936 4234 1936
Size: 2298 4594
SapI GCTCTTCn'nnn_
Cuts at: 2309 2678 4031 2309
Size: 369 1353 5170
Fragments arranged by size:
5170 1353 369
SbfI CC_TGCA'GG
Cuts at: 4244 4244
Size: 6892
ScaI AGT'ACT
Cuts at: 3193 5140 6852 3193
Size: 1947 1712 3233
Fragments arranged by size:
3233 1947 1712
SfcI C'TryA_G
Cuts at: 826 1045 2130 2170 2496 2835 3256 3324 3546
Size: 219 1085 40 326 339 421 68 222
Cuts at: 3546 4240 5375 6056 6247 826
Size: 694 1135 681 191 1471
Fragments arranged by size:
1471 1135 1085 694 681 421 339 326
222 219 191 68 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6892
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6892
SmaI CCC'GGG
Cuts at: 1872 3713 3949 4219 1872
Size: 1841 236 270 4545
Fragments arranged by size:
4545 1841 270 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 4996 5897 6159 820
Size: 197 926 597 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 4683 493
Size: 4190 2702
SpeI A'CTAG_T
Cuts at: 152 2740 3932 152
Size: 2588 1192 3112
Fragments arranged by size:
3112 2588 1192
SphI G_CATG'C
Cuts at: 1132 4250 1132
Size: 3118 3774
SspI AAT'ATT
Cuts at: 5 52 4159 4816 5
Size: 47 4107 657 2081
Fragments arranged by size:
4107 2081 657 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6892
StyI C'CwwG_G
Cuts at: 513 1065 4331 513
Size: 552 3266 3074
Fragments arranged by size:
3266 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4919 4936 5089 5274 5719 325
Size: 1255 3339 17 153 185 445 1498
Fragments arranged by size:
3339 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3191 5138 6850
Size: 274 80 33 51 1443 1214 1947 1712
Cuts at: 6850 96
Size: 138
Fragments arranged by size:
1947 1712 1443 1214 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3355 3387 6852 6884 3355
Size: 32 3465 32 3363
Fragments arranged by size:
3465 3363 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6892
XbaI T'CTAG_A
Cuts at: 2896 4228 2896
Size: 1332 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2063 3937 2063
Size: 1874 5018
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6892
XmaI C'CCGG_G
Cuts at: 1870 3711 3947 4217 1870
Size: 1841 236 270 4545
Fragments arranged by size:
4545 1841 270 236
XmnI GAAnn'nnTTC
Cuts at: 2357 2850 3036 3106 3229 5021 2357
Size: 493 186 70 123 1792 4228
Fragments arranged by size:
4228 1792 493 186 123 70
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4700 276
Size: 53 83 186 936 3166 2468
Fragments arranged by size:
3166 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI