Restriction map for
pFN21AB5784
(Circular) (Six-base) MAPSORT of: pFN21AB5784.seq Check: 9392 from: 1 to: 7015
pFN21AB5784 7015 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4003 4003
Size: 7015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4825 278
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 2987 4358 1937
Size: 726 324 1371 4594
Fragments arranged by size:
4594 1371 726 324
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3333 3387 3600 5078 6462 6730 1632
Size: 1701 54 213 1478 1384 268 1917
Fragments arranged by size:
1917 1701 1478 1384 268 213 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 2412 2658 5914 6891 1203
Size: 1209 246 3256 977 1327
Fragments arranged by size:
3256 1327 1209 977 246
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4011 1184
Size: 2827 4188
AlwNI CAG_nnn'CTG
Cuts at: 3713 3809 4758 6330 3713
Size: 96 949 1572 4398
Fragments arranged by size:
4398 1572 949 96
ApaI G_GGCC'C
Cuts at: 3009 3495 3728 3009
Size: 486 233 6296
Fragments arranged by size:
6296 486 233
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 2746 2971 4324 4550 4702 6811 1493
Size: 1253 225 1353 226 152 2109 1697
Fragments arranged by size:
2109 1697 1353 1253 226 225 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 4340 1870
Size: 73 2397 4545
Fragments arranged by size:
4545 2397 73
BamHI G'GATC_C
Cuts at: 4291 4345 4712 4291
Size: 54 367 6594
Fragments arranged by size:
6594 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3742 4336 5791 618
Size: 325 217 627 1955 594 1455 1842
Fragments arranged by size:
1955 1842 1455 627 594 325 217
BanII G_rGCy'C
Cuts at: 1391 3009 3495 3728 4334 1391
Size: 1618 486 233 606 4072
Fragments arranged by size:
4072 1618 606 486 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3339 3556 928
Size: 971 1440 217 4387
Fragments arranged by size:
4387 1440 971 217
BbvCI CC'TCA_GC
Cuts at: 3864 3864
Size: 7015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5206 5240 1903
Size: 34 3269 34 3678
Fragments arranged by size:
3678 3269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4907 6117 4907
Size: 1210 5805
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BfrBI ATG'CAT
Cuts at: 4202 4202
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4253 5626 136
Size: 107 122 71 631 605 2581 1373 1525
Fragments arranged by size:
2581 1525 1373 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3051 7010 3051
Size: 3959 3056
BlpI GC'TnA_GC
Cuts at: 4432 4432
Size: 7015
Bme1580I G_kGCm'C
Cuts at: 1790 3009 3495 3728 4005 5076 6232 1790
Size: 1219 486 233 277 1071 1156 2573
Fragments arranged by size:
2573 1219 1156 1071 486 277 233
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3479 4746
Size: 416 53 83 399 24 923 1139 1267
Cuts at: 4746 5704 442
Size: 958 1753
Fragments arranged by size:
1753 1267 1139 958 923 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2937 3690 3886 5675 1239
Size: 1698 753 196 1789 2579
Fragments arranged by size:
2579 1789 1698 753 196
Bpu10I CC'TnA_GC
Cuts at: 3081 3864 3081
Size: 783 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5140 6005 6303 5140
Size: 865 298 5852
Fragments arranged by size:
5852 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2262 5678 882
Size: 617 763 3416 2219
Fragments arranged by size:
3416 2219 763 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 4806 493
Size: 711 1209 2393 2702
Fragments arranged by size:
2702 2393 1209 711
BsaBI GATnn'nnATC
Cuts at: 2115 4116 7009 2115
Size: 2001 2893 2121
Fragments arranged by size:
2893 2121 2001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3413 4822 5204 5839
Size: 53 83 186 936 1880 1409 382 635
Cuts at: 5839 275
Size: 1451
Fragments arranged by size:
1880 1451 1409 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3060 5448 5846 6120 6267 6678 3060
Size: 2388 398 274 147 411 3397
Fragments arranged by size:
3397 2388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3460 3490 3688 3718 1178
Size: 30 165 30 2057 30 198 30 4475
Fragments arranged by size:
4475 2057 198 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2436 3375 3832 3849 3852 1186
Size: 1250 939 457 17 3 4349
Fragments arranged by size:
4349 1250 939 457 17 3
BseYI C'CCAG_C
Cuts at: 3117 6218 6714 3117
Size: 3101 496 3418
Fragments arranged by size:
3418 3101 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6442
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4465 5226 6254 1540
Size: 275 186 2464 761 1028 2301
Fragments arranged by size:
2464 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3478 5076 5161 6232 3478
Size: 1598 85 1071 4261
Fragments arranged by size:
4261 1598 1071 85
BsmI GAATG_Cn'
Cuts at: 4077 4096 4526 4619 4077
Size: 19 430 93 6473
Fragments arranged by size:
6473 430 93 19
BsmBI CGTCTCn'nnnn_
Cuts at: 3397 3397
Size: 7015
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3009 3478 3495 3728 4005 4334 5076
Size: 399 1219 469 17 233 277 329 742
Cuts at: 5076 5161 6232 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1219 1071 742 469 399 329 277
233 85 17
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 4904 6634 4904
Size: 1730 5285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4003 4370 844
Size: 3159 367 3489
Fragments arranged by size:
3489 3159 367
BsrBI CCG'CTC
Cuts at: 1394 1823 4902 1394
Size: 429 3079 3507
Fragments arranged by size:
3507 3079 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2373 5510 5684 65
Size: 1183 1125 3137 174 1396
Fragments arranged by size:
3137 1396 1183 1125 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5659 5846 1700
Size: 110 3849 187 2869
Fragments arranged by size:
3849 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 5075 6087 5075
Size: 1012 6003
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3749 794
Size: 2955 4060
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7015
BstXI CCAn_nnnn'nTGG
Cuts at: 3454 3454
Size: 7015
BstYI r'GATC_y
Cuts at: 1972 2280 2716 3051 3457 4291 4345 4712 5097
Size: 308 436 335 406 834 54 367 385
Cuts at: 5097 5114 6566 6652 6664 7010 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 834 436 406 385 367 346
335 308 86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2814 3531 4083 2814
Size: 717 552 5746
Fragments arranged by size:
5746 717 552
BtgI C'CryG_G
Cuts at: 513 1065 3092 3542 513
Size: 552 2027 450 3986
Fragments arranged by size:
3986 2027 552 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2154 487
Size: 683 56 928 5348
Fragments arranged by size:
5348 928 683 56
BtsI GCAGTG_nn'
Cuts at: 3132 3915 4531 5325 5345 3132
Size: 783 616 794 20 4802
Fragments arranged by size:
4802 794 783 616 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4222 4319 4222
Size: 97 6918
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3343 6022 809
Size: 507 2027 2679 1802
Fragments arranged by size:
2679 2027 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4462 5351 6980
Size: 54 1425 50 275 18 2632 889 1629
Cuts at: 6980 8
Size: 43
Fragments arranged by size:
2632 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4462 1537
Size: 275 2650 4090
Fragments arranged by size:
4090 2650 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 3319 3467 4945 1353
Size: 1449 517 148 1478 3423
Fragments arranged by size:
3423 1478 1449 517 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5602 5976 6122 387
Size: 1064 138 4013 374 146 1280
Fragments arranged by size:
4013 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2937 3333 3387 3600 3690 3886 5078
Size: 393 1305 396 54 213 90 196 1192
Cuts at: 5078 5675 6462 6730 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1305 1192 787 597 396 393 268
213 196 90 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3213 3534 3213
Size: 321 6694
EcoO109I rG'GnC_Cy
Cuts at: 2096 2587 3006 3491 3492 3724 3725 2096
Size: 491 419 485 1 232 1 5386
Fragments arranged by size:
5386 491 485 419 232 1 1
EcoRI G'AATT_C
Cuts at: 2746 2971 4324 4702 2746
Size: 225 1353 378 5059
Fragments arranged by size:
5059 1353 378 225
EcoRV GAT'ATC
Cuts at: 2704 2704
Size: 7015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 7015
FspI TGC'GCA
Cuts at: 5521 5521
Size: 7015
HaeII r_GCGC'y
Cuts at: 1678 6162 1678
Size: 4484 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 3038 3070 3592
Size: 32 135 32 1136 32 298 32 522
Cuts at: 3592 3624 5656 5688 5730 5762 1373
Size: 32 2032 32 42 32 2626
Fragments arranged by size:
2626 2032 1136 522 298 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 2635 3025 3611 3759 4359
Size: 988 281 352 345 390 586 148 600
Cuts at: 4359 4605 669
Size: 246 3079
Fragments arranged by size:
3079 988 600 586 390 352 345 281
246 148
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7015
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2711 3647 4003 6129 6313 1305
Size: 1406 936 356 2126 184 2007
Fragments arranged by size:
2126 2007 1406 936 356 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5193
Fragments arranged by size:
5193 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4973 5332 5491 518
Size: 648 3807 359 159 2042
Fragments arranged by size:
3807 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2995 3656 4431 5108 6256 6501 1606
Size: 643 746 661 775 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 746 677 661 643 245
NcoI C'CATG_G
Cuts at: 513 1065 3092 3542 513
Size: 552 2027 450 3986
Fragments arranged by size:
3986 2027 552 450
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NsiI A_TGCA'T
Cuts at: 4204 4204
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 2662 4373 5918 1132
Size: 97 1433 1711 1545 2229
Fragments arranged by size:
2229 1711 1545 1433 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7015
PciI A'CATG_T
Cuts at: 2658 5914 2658
Size: 3256 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3753 1108
Size: 2645 4370
PfoI T'CCnGG_A
Cuts at: 3589 3589
Size: 7015
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 5084 5116 6597 6629 3155
Size: 32 1897 32 1481 32 3541
Fragments arranged by size:
3541 1897 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PspOMI G'GGCC_C
Cuts at: 3005 3491 3724 3005
Size: 486 233 6296
Fragments arranged by size:
6296 486 233
PstI C_TGCA'G
Cuts at: 830 3444 4367 830
Size: 2614 923 3478
Fragments arranged by size:
3478 2614 923
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 2249 3656 2249
Size: 1407 5608
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SbfI CC_TGCA'GG
Cuts at: 4367 4367
Size: 7015
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 3432 3440 4363 5498 6179 6370 826
Size: 219 2387 8 923 1135 681 191 1471
Fragments arranged by size:
2387 1471 1135 923 681 219 191 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 4342 1872
Size: 2470 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5119 6020 6282 820
Size: 197 926 3176 901 262 1553
Fragments arranged by size:
3176 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 3796 4939 5
Size: 47 3744 1143 2081
Fragments arranged by size:
3744 2081 1143 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7015
StyI C'CwwG_G
Cuts at: 513 1065 3092 3542 3891 4454 513
Size: 552 2027 450 349 563 3074
Fragments arranged by size:
3074 2027 563 552 450 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 3433 3593 3760 5042 5059 5212
Size: 1255 664 1189 160 167 1282 17 153
Cuts at: 5212 5397 5842 325
Size: 185 445 1498
Fragments arranged by size:
1498 1282 1255 1189 664 445 185 167
160 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5261 6973 96
Size: 274 80 33 51 1443 3284 1712 138
Fragments arranged by size:
3284 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 4027 4059 6975 7007 2860
Size: 32 1135 32 2916 32 2868
Fragments arranged by size:
2916 2868 1135 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7015
XbaI T'CTAG_A
Cuts at: 2599 4351 2599
Size: 1752 5263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 4340 1870
Size: 2470 4545
XmnI GAAnn'nnTTC
Cuts at: 3167 4214 5144 3167
Size: 1047 930 5038
Fragments arranged by size:
5038 1047 930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4823 276
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI