Restriction map for
pFN21AB5783
(Circular) (Six-base) MAPSORT of: pFN21AB5783.seq Check: 2233 from: 1 to: 6790
pFN21AB5783 6790 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2860 3106 3349 2860
Size: 246 243 6301
Fragments arranged by size:
6301 246 243
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4600 278
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4133 1937
Size: 2196 4594
Acc65I G'GTAC_C
Cuts at: 2162 4111 2162
Size: 1949 4841
AclI AA'CG_TT
Cuts at: 1563 2556 4917 5290 1563
Size: 993 2361 373 3063
Fragments arranged by size:
3063 2361 993 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 3600 4010 4853 6237 6505 1632
Size: 524 1444 410 843 1384 268 1917
Fragments arranged by size:
1917 1444 1384 843 524 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6593
AflIII A'CryG_T
Cuts at: 1203 5689 6666 1203
Size: 4486 977 1327
Fragments arranged by size:
4486 1327 977
AgeI A'CCGG_T
Cuts at: 5621 5621
Size: 6790
AhdI GACnn_n'nnGTC
Cuts at: 2829 5519 2829
Size: 2690 4100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6790
AlwNI CAG_nnn'CTG
Cuts at: 3107 3455 3977 4533 6105 3107
Size: 348 522 556 1572 3792
Fragments arranged by size:
3792 1572 556 522 348
ApaI G_GGCC'C
Cuts at: 3466 3734 3466
Size: 268 6522
ApaLI G'TGCA_C
Cuts at: 4847 6003 4847
Size: 1156 5634
ApoI r'AATT_y
Cuts at: 1493 2519 3913 4099 4325 4477 6586 1493
Size: 1026 1394 186 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 1026 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 5344 160
Size: 872 4312 1606
Fragments arranged by size:
4312 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
AvaI C'yCGr_G
Cuts at: 1870 1943 2815 3086 3372 4115 1870
Size: 73 872 271 286 743 4545
Fragments arranged by size:
4545 872 743 286 271 73
BamHI G'GATC_C
Cuts at: 4120 4487 4120
Size: 367 6423
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 4111 5566 618
Size: 325 217 627 375 1949 1455 1842
Fragments arranged by size:
1949 1842 1455 627 375 325 217
BanII G_rGCy'C
Cuts at: 1391 2477 2822 3466 3594 3699 3734 4109 1391
Size: 1086 345 644 128 105 35 375 4072
Fragments arranged by size:
4072 1086 644 375 345 128 105 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2331 928
Size: 971 432 5387
Fragments arranged by size:
5387 971 432
BbvCI CC'TCA_GC
Cuts at: 3378 3456 3786 3378
Size: 78 330 6382
Fragments arranged by size:
6382 330 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4981 5015 1903
Size: 34 3044 34 3678
Fragments arranged by size:
3678 3044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2362 3220 4682 5892 2362
Size: 858 1462 1210 3260
Fragments arranged by size:
3260 1462 1210 858
BclI T'GATC_A
Cuts at: 1548 3402 3979 1548
Size: 1854 577 4359
Fragments arranged by size:
4359 1854 577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2726 3815 5401
Size: 107 122 71 631 605 1054 1089 1586
Cuts at: 5401 136
Size: 1525
Fragments arranged by size:
1586 1525 1089 1054 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6785 6785
Size: 6790
BlpI GC'TnA_GC
Cuts at: 4207 4207
Size: 6790
Bme1580I G_kGCm'C
Cuts at: 1790 2449 3466 3574 3734 4851 6007 1790
Size: 659 1017 108 160 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 1017 659 160 108
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6790
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2714 2745 4521
Size: 416 53 83 399 24 1297 31 1776
Cuts at: 4521 5479 442
Size: 958 1753
Fragments arranged by size:
1776 1753 1297 958 416 399 83 53
31 24
BmtI G_CTAG'C
Cuts at: 1056 3833 1056
Size: 2777 4013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3664 3664
Size: 6790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2617 3132 3432 5450 1239
Size: 1378 515 300 2018 2579
Fragments arranged by size:
2579 2018 1378 515 300
Bpu10I CC'TnA_GC
Cuts at: 3378 3456 3786 3378
Size: 78 330 6382
Fragments arranged by size:
6382 330 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4915 5780 6078 4915
Size: 865 298 5627
Fragments arranged by size:
5627 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3698 4029 5453 882
Size: 617 2199 331 1424 2219
Fragments arranged by size:
2219 2199 1424 617 331
BsaAI yAC'GTr
Cuts at: 493 1204 4581 493
Size: 711 3377 2702
Fragments arranged by size:
3377 2702 711
BsaBI GATnn'nnATC
Cuts at: 6784 6784
Size: 6790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4597 4979 5614 275
Size: 53 83 186 936 3064 382 635 1451
Fragments arranged by size:
3064 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3748 5223 5621 5895 6042 6453 3748
Size: 1475 398 274 147 411 4085
Fragments arranged by size:
4085 1475 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6565
Fragments arranged by size:
6565 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2813 3062 3332 3395 3407 3542 3602 3714
Size: 1627 249 270 63 12 135 60 112
Cuts at: 3714 1186
Size: 4262
Fragments arranged by size:
4262 1627 270 249 135 112 63 60
12
BseYI C'CCAG_C
Cuts at: 2086 5993 6489 2086
Size: 3907 496 2387
Fragments arranged by size:
3907 2387 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3582 3711 3786 1163
Size: 573 1846 129 75 4167
Fragments arranged by size:
4167 1846 573 129 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4240 5001 6029 1540
Size: 275 186 2239 761 1028 2301
Fragments arranged by size:
2301 2239 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2477 3594 4851 4936 6007 2477
Size: 1117 1257 85 1071 3260
Fragments arranged by size:
3260 1257 1117 1071 85
BsmI GAATG_Cn'
Cuts at: 4301 4394 4301
Size: 93 6697
BsmBI CGTCTCn'nnnn_
Cuts at: 2635 2862 2635
Size: 227 6563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2449 2477 2822 3466 3574 3594 3699
Size: 399 659 28 345 644 108 20 105
Cuts at: 3699 3734 4109 4851 4936 6007 1391
Size: 35 375 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 644 399 375 345
108 105 85 35 28 20
BspEI T'CCGG_A
Cuts at: 6453 6453
Size: 6790
BspHI T'CATG_A
Cuts at: 4679 6409 4679
Size: 1730 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2860 3092 3106 3349 4145 844
Size: 2016 232 14 243 796 3489
Fragments arranged by size:
3489 2016 796 243 232 14
BsrBI CCG'CTC
Cuts at: 1394 1823 4677 1394
Size: 429 2854 3507
Fragments arranged by size:
3507 2854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2772 3140 5285 5459 65
Size: 1183 1524 368 2145 174 1396
Fragments arranged by size:
2145 1524 1396 1183 368 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3184 5434 5621 1700
Size: 110 1374 2250 187 2869
Fragments arranged by size:
2869 2250 1374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6790
BssHII G'CGCG_C
Cuts at: 1925 2061 1925
Size: 136 6654
BssSI C'ACGA_G
Cuts at: 2342 4850 5862 2342
Size: 2508 1012 3270
Fragments arranged by size:
3270 2508 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3107 3569 794
Size: 2313 462 4015
Fragments arranged by size:
4015 2313 462
BstBI TT'CG_AA
Cuts at: 2121 5594 2121
Size: 3473 3317
BstEII G'GTnAC_C
Cuts at: 1184 2708 2880 1184
Size: 1524 172 5094
Fragments arranged by size:
5094 1524 172
BstXI CCAn_nnnn'nTGG
Cuts at: 2586 2586
Size: 6790
BstYI r'GATC_y
Cuts at: 1972 3061 4120 4487 4872 4889 6341 6427 6439
Size: 1089 1059 367 385 17 1452 86 12
Cuts at: 6439 6785 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1089 1059 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2796 3033 3609 2796
Size: 237 576 5977
Fragments arranged by size:
5977 576 237
BtgI C'CryG_G
Cuts at: 513 1065 2004 3810 513
Size: 552 939 1806 3493
Fragments arranged by size:
3493 1806 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 487
Size: 683 56 1166 4885
Fragments arranged by size:
4885 1166 683 56
BtsI GCAGTG_nn'
Cuts at: 3405 3890 4306 5100 5120 3405
Size: 485 416 794 20 5075
Fragments arranged by size:
5075 794 485 416 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6755
DraI TTT'AAA
Cuts at: 4094 4094
Size: 6790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5797 809
Size: 507 4481 1802
Fragments arranged by size:
4481 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4237 5126
Size: 54 1425 50 275 18 177 2230 889
Cuts at: 5126 6755 8
Size: 1629 43
Fragments arranged by size:
2230 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4237 1537
Size: 275 2425 4090
Fragments arranged by size:
4090 2425 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2541 2565 3654 4720 1353
Size: 1188 24 1089 1066 3423
Fragments arranged by size:
3423 1188 1089 1066 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3500 5377 5751 5897 387
Size: 1064 138 1911 1877 374 146 1280
Fragments arranged by size:
1911 1877 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2475 3592 2475
Size: 1117 5673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2617 3132 3432 3600 4010 4853
Size: 393 524 461 515 300 168 410 843
Cuts at: 4853 5450 6237 6505 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 843 787 597 524 515 461 410
393 300 268 168
EcoNI CCTnn'n_nnAGG
Cuts at: 3934 3934
Size: 6790
EcoO109I rG'GnC_Cy
Cuts at: 2074 2352 2762 2903 3151 3462 3463 3731 3781
Size: 278 410 141 248 311 1 268 50
Cuts at: 3781 2074
Size: 5083
Fragments arranged by size:
5083 410 311 278 268 248 141 50
1
EcoRI G'AATT_C
Cuts at: 4099 4477 4099
Size: 378 6412
FspI TGC'GCA
Cuts at: 5296 5296
Size: 6790
HaeII r_GCGC'y
Cuts at: 1678 2750 5937 1678
Size: 1072 3187 2531
Fragments arranged by size:
3187 2531 1072
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 2815 2847 5431
Size: 32 135 32 992 32 219 32 2584
Cuts at: 5431 5463 5505 5537 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2584 992 219 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 4134 4380 669
Size: 988 281 622 1574 246 3079
Fragments arranged by size:
3079 1574 988 622 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6790
HpaI GTT'AAC
Cuts at: 4380 4380
Size: 6790
KpnI G_GTAC'C
Cuts at: 2166 4115 2166
Size: 1949 4841
MfeI C'AATT_G
Cuts at: 4389 4389
Size: 6790
MluI A'CGCG_T
Cuts at: 6666 6666
Size: 6790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 3415 3675 5904 6088 1305
Size: 1276 834 260 2229 184 2007
Fragments arranged by size:
2229 2007 1276 834 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4968
Fragments arranged by size:
4968 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2584 4748 5107 5266 518
Size: 648 1418 2164 359 159 2042
Fragments arranged by size:
2164 2042 1418 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3431 3455 4206 4883 6031 6276 1606
Size: 1825 24 751 677 1148 245 2120
Fragments arranged by size:
2120 1825 1148 751 677 245 24
NaeI GCC'GGC
Cuts at: 3186 3186
Size: 6790
NcoI C'CATG_G
Cuts at: 513 1065 2004 3810 513
Size: 552 939 1806 3493
Fragments arranged by size:
3493 1806 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6790
NgoMIV G'CCGG_C
Cuts at: 3184 3184
Size: 6790
NheI G'CTAG_C
Cuts at: 1052 3829 1052
Size: 2777 4013
NotI GC'GGCC_GC
Cuts at: 4237 4237
Size: 6790
NspI r_CATG'y
Cuts at: 1132 1229 2215 4148 5693 1132
Size: 97 986 1933 1545 2229
Fragments arranged by size:
2229 1933 1545 986 97
PasI CC'CwG_GG
Cuts at: 1348 3147 1348
Size: 1799 4991
PciI A'CATG_T
Cuts at: 5689 5689
Size: 6790
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 2458 1108
Size: 978 372 5440
Fragments arranged by size:
5440 978 372
PfoI T'CCnGG_A
Cuts at: 3777 3777
Size: 6790
PmeI GTTT'AAAC
Cuts at: 4094 4094
Size: 6790
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 6372 6404 4859
Size: 32 1481 32 5245
Fragments arranged by size:
5245 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2352 2762 3151 3781 2074
Size: 278 410 389 630 5083
Fragments arranged by size:
5083 630 410 389 278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6790
PsiI TTA'TAA
Cuts at: 4360 4360
Size: 6790
PspOMI G'GGCC_C
Cuts at: 3462 3730 3462
Size: 268 6522
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3915 3947 3915
Size: 32 6758
PstI C_TGCA'G
Cuts at: 830 2472 2952 3103 4142 830
Size: 1642 480 151 1039 3478
Fragments arranged by size:
3478 1642 1039 480 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6790
PvuII CAG'CTG
Cuts at: 3431 3455 3431
Size: 24 6766
SacI G_AGCT'C
Cuts at: 2477 3594 2477
Size: 1117 5673
SalI G'TCGA_C
Cuts at: 1936 4132 1936
Size: 2196 4594
SbfI CC_TGCA'GG
Cuts at: 3103 4142 3103
Size: 1039 5751
ScaI AGT'ACT
Cuts at: 5038 6750 5038
Size: 1712 5078
SfcI C'TryA_G
Cuts at: 826 1045 2468 2948 3099 3684 4138 5273 5954
Size: 219 1423 480 151 585 454 1135 681
Cuts at: 5954 6145 826
Size: 191 1471
Fragments arranged by size:
1471 1423 1135 681 585 480 454 219
191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6790
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6790
SmaI CCC'GGG
Cuts at: 1872 4117 1872
Size: 2245 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4894 5795 6057 820
Size: 197 926 2951 901 262 1553
Fragments arranged by size:
2951 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4581 493
Size: 4088 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6790
SphI G_CATG'C
Cuts at: 1132 2215 4148 1132
Size: 1083 1933 3774
Fragments arranged by size:
3774 1933 1083
SspI AAT'ATT
Cuts at: 5 52 4714 5
Size: 47 4662 2081
Fragments arranged by size:
4662 2081 47
StuI AGG'CCT
Cuts at: 1517 2222 2729 3848 4056 1517
Size: 705 507 1119 208 4251
Fragments arranged by size:
4251 1119 705 507 208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2740 3358 3441 3699 3810 4229
Size: 552 939 736 618 83 258 111 419
Cuts at: 4229 513
Size: 3074
Fragments arranged by size:
3074 939 736 618 552 419 258 111
83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 2868 3142 3313 4817 4834 4987
Size: 1255 1116 172 274 171 1504 17 153
Cuts at: 4987 5172 5617 325
Size: 185 445 1498
Fragments arranged by size:
1504 1498 1255 1116 445 274 185 172
171 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4029 5036 6748
Size: 274 80 33 51 1443 2052 1007 1712
Cuts at: 6748 96
Size: 138
Fragments arranged by size:
2052 1712 1443 1007 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3429 3461 6750 6782 3429
Size: 32 3289 32 3437
Fragments arranged by size:
3437 3289 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6790
XbaI T'CTAG_A
Cuts at: 4126 4126
Size: 6790
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6790
XmaI C'CCGG_G
Cuts at: 1870 4115 1870
Size: 2245 4545
XmnI GAAnn'nnTTC
Cuts at: 2520 4919 2520
Size: 2399 4391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4598 276
Size: 53 83 186 936 3064 2468
Fragments arranged by size:
3064 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI