Restriction map for
pFN21AB5782
(Circular) (Six-base) MAPSORT of: pFN21AB5782.seq Check: 8310 from: 1 to: 7012
pFN21AB5782 7012 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3405 3405
Size: 7012
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3576 4822 278
Size: 53 83 186 936 2040 1246 2468
Fragments arranged by size:
2468 2040 1246 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4355 1937
Size: 2418 4594
Acc65I G'GTAC_C
Cuts at: 4333 4333
Size: 7012
AclI AA'CG_TT
Cuts at: 1563 5139 5512 1563
Size: 3576 373 3063
Fragments arranged by size:
3576 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2599 2648 3920 5075 6459 6727 1632
Size: 967 49 1272 1155 1384 268 1917
Fragments arranged by size:
1917 1384 1272 1155 967 268 49
AfeI AGC'GCT
Cuts at: 3632 3632
Size: 7012
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6815
AflIII A'CryG_T
Cuts at: 1203 5911 6888 1203
Size: 4708 977 1327
Fragments arranged by size:
4708 1327 977
AgeI A'CCGG_T
Cuts at: 5843 5843
Size: 7012
AhdI GACnn_n'nnGTC
Cuts at: 5741 5741
Size: 7012
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4175 1184
Size: 2991 4021
AlwNI CAG_nnn'CTG
Cuts at: 2100 2149 3378 3481 3556 3987 4755 6327 2100
Size: 49 1229 103 75 431 768 1572 2785
Fragments arranged by size:
2785 1572 1229 768 431 103 75 49
ApaI G_GGCC'C
Cuts at: 2127 2473 3917 2127
Size: 346 1444 5222
Fragments arranged by size:
5222 1444 346
ApaLI G'TGCA_C
Cuts at: 2077 5069 6225 2077
Size: 2992 1156 2864
Fragments arranged by size:
2992 2864 1156
ApoI r'AATT_y
Cuts at: 1493 2897 4321 4547 4699 6808 1493
Size: 1404 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 1404 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5566 160
Size: 872 4534 1606
Fragments arranged by size:
4534 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2702 3658 3700 4337 1870
Size: 73 111 648 956 42 637 4545
Fragments arranged by size:
4545 956 648 637 111 73 42
BamHI G'GATC_C
Cuts at: 2170 4342 4709 2170
Size: 2172 367 4473
Fragments arranged by size:
4473 2172 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2139 2831 3348 3789 4124
Size: 325 217 627 352 692 517 441 335
Cuts at: 4124 4333 5788 618
Size: 209 1455 1842
Fragments arranged by size:
1842 1455 692 627 517 441 352 335
325 217 209
BanII G_rGCy'C
Cuts at: 1391 2127 2473 3672 3716 3917 4331 1391
Size: 736 346 1199 44 201 414 4072
Fragments arranged by size:
4072 1199 736 414 346 201 44
BbeI G_GCGC'C
Cuts at: 2835 2835
Size: 7012
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2277 2993 3097 928
Size: 971 378 716 104 4843
Fragments arranged by size:
4843 971 716 378 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2488 2522 2556 2590 2881
Size: 34 50 34 467 34 34 34 291
Cuts at: 2881 2915 5203 5237 1903
Size: 34 2288 34 3678
Fragments arranged by size:
3678 2288 467 291 50 34 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4904 6114 4904
Size: 1210 5802
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7012
BfrBI ATG'CAT
Cuts at: 2112 2112
Size: 7012
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2502 3702 3819
Size: 107 122 71 631 605 830 1200 117
Cuts at: 3819 5623 136
Size: 1804 1525
Fragments arranged by size:
1804 1525 1200 830 631 605 122 117
107 71
BglII A'GATC_T
Cuts at: 7007 7007
Size: 7012
BlpI GC'TnA_GC
Cuts at: 3715 4429 3715
Size: 714 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2127 2473 3851 3917 4127 5073 6229
Size: 291 46 346 1378 66 210 946 1156
Cuts at: 6229 1790
Size: 2573
Fragments arranged by size:
2573 1378 1156 946 346 291 210 66
46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7012
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3328 4234 4743
Size: 416 53 83 399 24 1911 906 509
Cuts at: 4743 5701 442
Size: 958 1753
Fragments arranged by size:
1911 1753 958 906 509 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7012
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4018 4018
Size: 7012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 3034 3082 4052 5672 1239
Size: 1649 146 48 970 1620 2579
Fragments arranged by size:
2579 1649 1620 970 146 48
Bpu10I CC'TnA_GC
Cuts at: 3523 4190 3523
Size: 667 6345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4217 5137 6002 6300 4217
Size: 920 865 298 4929
Fragments arranged by size:
4929 920 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3360 5675 882
Size: 617 1861 2315 2219
Fragments arranged by size:
2315 2219 1861 617
BsaAI yAC'GTr
Cuts at: 493 1204 2623 4803 493
Size: 711 1419 2180 2702
Fragments arranged by size:
2702 2180 1419 711
BsaBI GATnn'nnATC
Cuts at: 7006 7006
Size: 7012
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 2832 3573 4819
Size: 53 83 186 936 1185 114 741 1246
Cuts at: 4819 5201 5836 275
Size: 382 635 1451
Fragments arranged by size:
1451 1246 1185 936 741 635 382 186
114 83 53
BsaWI w'CCGG_w
Cuts at: 2684 5445 5843 6117 6264 6675 2684
Size: 2761 398 274 147 411 3021
Fragments arranged by size:
3021 2761 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 3263 3293 3494
Size: 30 165 30 1176 30 654 30 201
Cuts at: 3494 3524 1178
Size: 30 4666
Fragments arranged by size:
4666 1176 654 201 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2902 3085 1186
Size: 1716 183 5113
Fragments arranged by size:
5113 1716 183
BseYI C'CCAG_C
Cuts at: 2008 2827 3295 3529 4014 6215 6711 2008
Size: 819 468 234 485 2201 496 2309
Fragments arranged by size:
2309 2201 819 496 485 468 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2116 3233 3341 1163
Size: 573 380 1117 108 4834
Fragments arranged by size:
4834 1117 573 380 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2497 2857 4462 5223 6251 1540
Size: 275 186 496 360 1605 761 1028 2301
Fragments arranged by size:
2301 1605 1028 761 496 360 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 5073 5158 6229 2081
Size: 2992 85 1071 2864
Fragments arranged by size:
2992 2864 1071 85
BsmI GAATG_Cn'
Cuts at: 2114 3082 4152 4523 4616 2114
Size: 968 1070 371 93 4510
Fragments arranged by size:
4510 1070 968 371 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2127 2473 3672 3716 3851 3917
Size: 399 291 46 346 1199 44 135 66
Cuts at: 3917 4127 4331 5073 5158 6229 1391
Size: 210 204 742 85 1071 2174
Fragments arranged by size:
2174 1199 1071 742 399 346 291 210
204 135 85 66 46 44
BspEI T'CCGG_A
Cuts at: 2684 6675 2684
Size: 3991 3021
BspHI T'CATG_A
Cuts at: 2211 4287 4901 6631 2211
Size: 2076 614 1730 2592
Fragments arranged by size:
2592 2076 1730 614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 2469 2719 3313 3405 3995 4367 844
Size: 1487 138 250 594 92 590 372 3489
Fragments arranged by size:
3489 1487 594 590 372 250 138 92
BsrBI CCG'CTC
Cuts at: 1394 1823 4899 1394
Size: 429 3076 3507
Fragments arranged by size:
3507 3076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3061 3172 5507 5681 65
Size: 1183 1813 111 2335 174 1396
Fragments arranged by size:
2335 1813 1396 1183 174 111
BsrFI r'CCGG_y
Cuts at: 1700 1810 3345 3786 5656 5843 1700
Size: 110 1535 441 1870 187 2869
Fragments arranged by size:
2869 1870 1535 441 187 110
BsrGI T'GTAC_A
Cuts at: 96 2880 96
Size: 2784 4228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7012
BssSI C'ACGA_G
Cuts at: 5072 6084 5072
Size: 1012 6000
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2139 3378 3804 3987 794
Size: 1345 1239 426 183 3819
Fragments arranged by size:
3819 1345 1239 426 183
BstBI TT'CG_AA
Cuts at: 3906 5816 3906
Size: 1910 5102
BstEII G'GTnAC_C
Cuts at: 1184 2968 1184
Size: 1784 5228
BstXI CCAn_nnnn'nTGG
Cuts at: 2980 3058 2980
Size: 78 6934
BstYI r'GATC_y
Cuts at: 1972 2170 4342 4709 5094 5111 6563 6649 6661
Size: 198 2172 367 385 17 1452 86 12
Cuts at: 6661 7007 1972
Size: 346 1977
Fragments arranged by size:
2172 1977 1452 385 367 346 198 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2432 2432
Size: 7012
BtgI C'CryG_G
Cuts at: 513 1065 3027 3382 3758 4128 513
Size: 552 1962 355 376 370 3397
Fragments arranged by size:
3397 1962 552 376 370 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4090 487
Size: 683 56 2864 3409
Fragments arranged by size:
3409 2864 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 3557 4528 5322 5342 2024
Size: 1533 971 794 20 3694
Fragments arranged by size:
3694 1533 971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7012
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6977
DraI TTT'AAA
Cuts at: 4316 4316
Size: 7012
DraIII CAC_nnn'GTG
Cuts at: 2139 3261 3869 2139
Size: 1122 608 5282
Fragments arranged by size:
5282 1122 608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6019 809
Size: 507 4703 1802
Fragments arranged by size:
4703 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2198 2494 2854
Size: 54 1425 50 275 18 368 296 360
Cuts at: 2854 3252 3640 3784 4459 5348 6977 8
Size: 398 388 144 675 889 1629 43
Fragments arranged by size:
1629 1425 889 675 398 388 368 360
296 275 144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2494 2854 4459 1537
Size: 275 682 360 1605 4090
Fragments arranged by size:
4090 1605 682 360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4942 1353
Size: 3589 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5599 5973 6119 387
Size: 1064 138 4010 374 146 1280
Fragments arranged by size:
4010 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2599 2648 2888 3034 3082 3920 4052
Size: 393 967 49 240 146 48 838 132
Cuts at: 4052 5075 5672 6459 6727 1239
Size: 1023 597 787 268 1524
Fragments arranged by size:
1524 1023 967 838 787 597 393 268
240 146 132 49 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 2069
Size: 7012
EcoO109I rG'GnC_Cy
Cuts at: 2123 2470 2511 3325 3913 3914 3984 4097 2123
Size: 347 41 814 588 1 70 113 5038
Fragments arranged by size:
5038 814 588 347 113 70 41 1
EcoRI G'AATT_C
Cuts at: 2897 4321 4699 2897
Size: 1424 378 5210
Fragments arranged by size:
5210 1424 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2333 3412 3934 2170
Size: 163 1079 522 5248
Fragments arranged by size:
5248 1079 522 163
FspI TGC'GCA
Cuts at: 5518 5518
Size: 7012
HaeII r_GCGC'y
Cuts at: 1678 2835 3296 3634 4216 4275 6159 1678
Size: 1157 461 338 582 59 1884 2531
Fragments arranged by size:
2531 1884 1157 582 461 338 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2894 2926 3494
Size: 32 135 32 590 32 700 32 568
Cuts at: 3494 3526 5653 5685 5727 5759 1373
Size: 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 700 590 568 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4356 4602 669
Size: 988 281 2418 246 3079
Fragments arranged by size:
3079 2418 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7012
HpaI GTT'AAC
Cuts at: 4602 4602
Size: 7012
KasI G'GCGC_C
Cuts at: 2831 2831
Size: 7012
KpnI G_GTAC'C
Cuts at: 4337 4337
Size: 7012
MfeI C'AATT_G
Cuts at: 4611 4611
Size: 7012
MluI A'CGCG_T
Cuts at: 6888 6888
Size: 7012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 6126 6310 1305
Size: 1365 3456 184 2007
Fragments arranged by size:
3456 2007 1365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5190
Fragments arranged by size:
5190 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4067 4970 5329 5488 518
Size: 648 2901 903 359 159 2042
Fragments arranged by size:
2901 2042 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2206 2296 2677 2859 2923 3693 3760
Size: 494 106 90 381 182 64 770 67
Cuts at: 3760 3795 3829 3958 4428 5105 6253 6498 1606
Size: 35 34 129 470 677 1148 245 2120
Fragments arranged by size:
2120 1148 770 677 494 470 381 245
182 129 106 90 67 64 35 34
NarI GG'CG_CC
Cuts at: 2832 2832
Size: 7012
NcoI C'CATG_G
Cuts at: 513 1065 3382 513
Size: 552 2317 4143
Fragments arranged by size:
4143 2317 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7012
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7012
NotI GC'GGCC_GC
Cuts at: 4459 4459
Size: 7012
NsiI A_TGCA'T
Cuts at: 2114 2114
Size: 7012
NspI r_CATG'y
Cuts at: 1132 1229 4370 5915 1132
Size: 97 3141 1545 2229
Fragments arranged by size:
3141 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7012
PciI A'CATG_T
Cuts at: 5911 5911
Size: 7012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3481 1108
Size: 2373 4639
PfoI T'CCnGG_A
Cuts at: 3108 3267 4200 3108
Size: 159 933 5920
Fragments arranged by size:
5920 933 159
PmeI GTTT'AAAC
Cuts at: 4316 4316
Size: 7012
PmlI CAC'GTG
Cuts at: 2623 2623
Size: 7012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5081 5113 6594 6626 5081
Size: 32 1481 32 5467
Fragments arranged by size:
5467 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2511 3325 3984 2511
Size: 814 659 5539
Fragments arranged by size:
5539 814 659
PshAI GACnn'nnGTC
Cuts at: 1613 2663 1613
Size: 1050 5962
PsiI TTA'TAA
Cuts at: 4582 4582
Size: 7012
PspOMI G'GGCC_C
Cuts at: 2123 2469 3913 2123
Size: 346 1444 5222
Fragments arranged by size:
5222 1444 346
PstI C_TGCA'G
Cuts at: 830 3164 3416 3935 3957 4364 830
Size: 2334 252 519 22 407 3478
Fragments arranged by size:
3478 2334 519 407 252 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7012
PvuII CAG'CTG
Cuts at: 2100 2206 2296 2677 2923 3829 3958 2100
Size: 106 90 381 246 906 129 5154
Fragments arranged by size:
5154 906 381 246 129 106 90
SacII CC_GC'GG
Cuts at: 3761 3761
Size: 7012
SalI G'TCGA_C
Cuts at: 1936 4354 1936
Size: 2418 4594
SbfI CC_TGCA'GG
Cuts at: 4364 4364
Size: 7012
ScaI AGT'ACT
Cuts at: 3602 5260 6972 3602
Size: 1658 1712 3642
Fragments arranged by size:
3642 1712 1658
SexAI A'CCwGG_T
Cuts at: 2135 2135
Size: 7012
SfcI C'TryA_G
Cuts at: 826 1045 3160 3412 3931 3953 4360 5495 6176
Size: 219 2115 252 519 22 407 1135 681
Cuts at: 6176 6367 826
Size: 191 1471
Fragments arranged by size:
2115 1471 1135 681 519 407 252 219
191 22
SfoI GGC'GCC
Cuts at: 2833 2833
Size: 7012
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7012
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7012
SmaI CCC'GGG
Cuts at: 1872 3660 3702 4339 1872
Size: 1788 42 637 4545
Fragments arranged by size:
4545 1788 637 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4232 5116 6017 6279 820
Size: 197 926 2289 884 901 262 1553
Fragments arranged by size:
2289 1553 926 901 884 262 197
SnaBI TAC'GTA
Cuts at: 493 4803 493
Size: 4310 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7012
SphI G_CATG'C
Cuts at: 1132 4370 1132
Size: 3238 3774
SspI AAT'ATT
Cuts at: 5 52 4936 5
Size: 47 4884 2081
Fragments arranged by size:
4884 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7012
StyI C'CwwG_G
Cuts at: 513 1065 2370 3382 4451 513
Size: 552 1305 1012 1069 3074
Fragments arranged by size:
3074 1305 1069 1012 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2675 4006 5039 5056 5209 5394 5839
Size: 1255 1095 1331 1033 17 153 185 445
Cuts at: 5839 325
Size: 1498
Fragments arranged by size:
1498 1331 1255 1095 1033 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 2880 3600
Size: 274 80 33 51 1443 414 489 720
Cuts at: 3600 3876 4206 5258 6970 96
Size: 276 330 1052 1712 138
Fragments arranged by size:
1712 1443 1052 720 489 414 330 276
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 3430 3462 6972 7004 2579
Size: 32 819 32 3510 32 2587
Fragments arranged by size:
3510 2587 819 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3539 1316
Size: 2223 4789
XbaI T'CTAG_A
Cuts at: 4348 4348
Size: 7012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3025 3106 3025
Size: 81 6931
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7012
XmaI C'CCGG_G
Cuts at: 1870 3658 3700 4337 1870
Size: 1788 42 637 4545
Fragments arranged by size:
4545 1788 637 42
XmnI GAAnn'nnTTC
Cuts at: 5141 5141
Size: 7012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3574 4820 276
Size: 53 83 186 936 2040 1246 2468
Fragments arranged by size:
2468 2040 1246 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI
SwaI