(Circular) (Six-base) MAPSORT of: pFN21AB5781.seq Check: 329 from: 1 to: 7183 pFN21AB5781 7183 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3595 3595 Size: 7183 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4993 278 Size: 53 83 186 936 3457 2468 Fragments arranged by size: 3457 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4526 1937 Size: 2589 4594 Acc65I G'GTAC_C Cuts at: 4504 4504 Size: 7183 AclI AA'CG_TT Cuts at: 1563 5310 5683 1563 Size: 3747 373 3063 Fragments arranged by size: 3747 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 3699 5246 6630 6898 1632 Size: 812 1255 1547 1384 268 1917 Fragments arranged by size: 1917 1547 1384 1255 812 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6986 AflIII A'CryG_T Cuts at: 1203 2034 6082 7059 1203 Size: 831 4048 977 1327 Fragments arranged by size: 4048 1327 977 831 AgeI A'CCGG_T Cuts at: 6014 6014 Size: 7183 AhdI GACnn_n'nnGTC Cuts at: 5912 5912 Size: 7183 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3312 1184 Size: 2128 5055 AlwNI CAG_nnn'CTG Cuts at: 2375 2414 4188 4926 6498 2375 Size: 39 1774 738 1572 3060 Fragments arranged by size: 3060 1774 1572 738 39 ApaI G_GGCC'C Cuts at: 2088 2088 Size: 7183 ApaLI G'TGCA_C Cuts at: 4004 4476 5240 6396 4004 Size: 472 764 1156 4791 Fragments arranged by size: 4791 1156 764 472 ApoI r'AATT_y Cuts at: 1493 2562 3206 3409 3421 3829 4492 4718 4870 Size: 1069 644 203 12 408 663 226 152 Cuts at: 4870 6979 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1069 663 644 408 226 203 152 12 AseI AT'TA_AT Cuts at: 160 1032 3345 5737 160 Size: 872 2313 2392 1606 Fragments arranged by size: 2392 2313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 AvaI C'yCGr_G Cuts at: 1870 1943 2181 4508 1870 Size: 73 238 2327 4545 Fragments arranged by size: 4545 2327 238 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2511 2544 3302 3335 2511 Size: 33 758 33 6359 Fragments arranged by size: 6359 758 33 33 BamHI G'GATC_C Cuts at: 3193 4513 4880 3193 Size: 1320 367 5496 Fragments arranged by size: 5496 1320 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 4504 5959 618 Size: 325 217 627 224 2493 1455 1842 Fragments arranged by size: 2493 1842 1455 627 325 224 217 BanII G_rGCy'C Cuts at: 1391 2081 2088 2965 4502 1391 Size: 690 7 877 1537 4072 Fragments arranged by size: 4072 1537 877 690 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6212 BbvCI CC'TCA_GC Cuts at: 3754 3754 Size: 7183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2172 2206 5374 5408 1903 Size: 34 235 34 3168 34 3678 Fragments arranged by size: 3678 3168 235 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3931 5075 6285 3931 Size: 1144 1210 4829 Fragments arranged by size: 4829 1210 1144 BclI T'GATC_A Cuts at: 1548 2587 3069 3229 4276 1548 Size: 1039 482 160 1047 4455 Fragments arranged by size: 4455 1047 1039 482 160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5794 136 Size: 107 122 71 631 605 337 3785 1525 Fragments arranged by size: 3785 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2665 7178 2665 Size: 4513 2670 BlpI GC'TnA_GC Cuts at: 4600 4600 Size: 7183 Bme1580I G_kGCm'C Cuts at: 1790 2088 3702 4008 4480 5244 6400 1790 Size: 298 1614 306 472 764 1156 2573 Fragments arranged by size: 2573 1614 1156 764 472 306 298 BmgBI CAC'GTC Cuts at: 1318 2112 4159 1318 Size: 794 2047 4342 Fragments arranged by size: 4342 2047 794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2281 2866 4071 Size: 416 53 83 399 24 864 585 1205 Cuts at: 4071 4187 4914 5872 442 Size: 116 727 958 1753 Fragments arranged by size: 1753 1205 958 864 727 585 416 399 116 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2843 1056 Size: 1787 5396 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4120 4120 Size: 7183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2095 2265 2849 2949 3126 3387 5843 Size: 803 53 170 584 100 177 261 2456 Cuts at: 5843 1239 Size: 2579 Fragments arranged by size: 2579 2456 803 584 261 177 170 100 53 Bpu10I CC'TnA_GC Cuts at: 3754 3754 Size: 7183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5308 6173 6471 5308 Size: 865 298 6020 Fragments arranged by size: 6020 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5846 882 Size: 617 4347 2219 Fragments arranged by size: 4347 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 4219 4974 493 Size: 711 831 2184 755 2702 Fragments arranged by size: 2702 2184 831 755 711 BsaBI GATnn'nnATC Cuts at: 2926 3477 7177 2926 Size: 551 3700 2932 Fragments arranged by size: 3700 2932 551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4990 5372 6007 275 Size: 53 83 186 936 3457 382 635 1451 Fragments arranged by size: 3457 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5616 6014 6288 6435 6846 5616 Size: 398 274 147 411 5953 Fragments arranged by size: 5953 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3483 3513 3622 3652 1178 Size: 30 165 30 2080 30 109 30 4709 Fragments arranged by size: 4709 2080 165 109 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2374 2652 3584 4135 1186 Size: 1188 278 932 551 4234 Fragments arranged by size: 4234 1188 932 551 278 BseYI C'CCAG_C Cuts at: 6386 6882 6386 Size: 496 6687 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3384 3845 1163 Size: 573 1648 461 4501 Fragments arranged by size: 4501 1648 573 461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4633 5394 6422 1540 Size: 275 186 2632 761 1028 2301 Fragments arranged by size: 2632 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2081 2130 3462 4008 4128 4200 4480 5244 5329 Size: 49 1332 546 120 72 280 764 85 Cuts at: 5329 6400 2081 Size: 1071 2864 Fragments arranged by size: 2864 1332 1071 764 546 280 120 85 72 49 BsmI GAATG_Cn' Cuts at: 4694 4787 4694 Size: 93 7090 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2088 2130 2965 3462 3702 4008 Size: 399 291 7 42 835 497 240 306 Cuts at: 4008 4128 4200 4480 4502 5244 5329 6400 1391 Size: 120 72 280 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 835 742 497 399 306 291 280 240 120 85 72 42 22 7 BspEI T'CCGG_A Cuts at: 6846 6846 Size: 7183 BspHI T'CATG_A Cuts at: 2848 5072 6802 2848 Size: 2224 1730 3229 Fragments arranged by size: 3229 2224 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3595 4538 844 Size: 2751 943 3489 Fragments arranged by size: 3489 2751 943 BsrBI CCG'CTC Cuts at: 1394 1823 5070 1394 Size: 429 3247 3507 Fragments arranged by size: 3507 3247 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2554 3072 3345 5678 5852 65 Size: 1183 1306 518 273 2333 174 1396 Fragments arranged by size: 2333 1396 1306 1183 518 273 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2843 5827 6014 1700 Size: 110 1033 2984 187 2869 Fragments arranged by size: 2984 2869 1033 187 110 BsrGI T'GTAC_A Cuts at: 96 2384 96 Size: 2288 4895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7183 BssSI C'ACGA_G Cuts at: 5243 6255 5243 Size: 1012 6171 BstAPI GCAn_nnn'nTGC Cuts at: 794 2115 2414 4406 794 Size: 1321 299 1992 3571 Fragments arranged by size: 3571 1992 1321 299 BstBI TT'CG_AA Cuts at: 5987 5987 Size: 7183 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7183 BstXI CCAn_nnnn'nTGG Cuts at: 3135 3135 Size: 7183 BstYI r'GATC_y Cuts at: 1972 2249 2371 2665 3193 4513 4880 5265 5282 Size: 277 122 294 528 1320 367 385 17 Cuts at: 5282 6734 6820 6832 7178 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1320 528 385 367 346 294 277 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2937 3088 3254 513 Size: 552 939 117 816 151 166 4442 Fragments arranged by size: 4442 939 816 552 166 151 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6444 Fragments arranged by size: 6444 683 56 BtsI GCAGTG_nn' Cuts at: 2310 4381 4699 5493 5513 2310 Size: 2071 318 794 20 3980 Fragments arranged by size: 3980 2071 794 318 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7183 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7148 DraI TTT'AAA Cuts at: 2298 2469 4487 2298 Size: 171 2018 4994 Fragments arranged by size: 4994 2018 171 DraIII CAC_nnn'GTG Cuts at: 4450 4450 Size: 7183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6190 809 Size: 507 4874 1802 Fragments arranged by size: 4874 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4630 5519 7148 Size: 54 1425 50 275 18 2800 889 1629 Cuts at: 7148 8 Size: 43 Fragments arranged by size: 2800 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4630 1537 Size: 275 2818 4090 Fragments arranged by size: 4090 2818 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2967 3254 3561 5113 1353 Size: 1614 287 307 1552 3423 Fragments arranged by size: 3423 1614 1552 307 287 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5770 6144 6290 387 Size: 1064 138 4181 374 146 1280 Fragments arranged by size: 4181 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2079 2079 Size: 7183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2095 2265 2444 2849 2949 3126 Size: 393 410 53 170 179 405 100 177 Cuts at: 3126 3387 3699 5246 5843 6630 6898 1239 Size: 261 312 1547 597 787 268 1524 Fragments arranged by size: 1547 1524 787 597 410 405 393 312 268 261 179 177 170 100 53 EcoO109I rG'GnC_Cy Cuts at: 2327 2327 Size: 7183 EcoRI G'AATT_C Cuts at: 2562 4492 4870 2562 Size: 1930 378 4875 Fragments arranged by size: 4875 1930 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4072 4270 4072 Size: 198 6985 FspI TGC'GCA Cuts at: 5689 5689 Size: 7183 HaeII r_GCGC'y Cuts at: 1678 6330 1678 Size: 4652 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 2960 2992 3061 Size: 32 135 32 784 32 572 32 69 Cuts at: 3061 3093 3483 3515 3929 3961 5824 5856 5898 Size: 32 390 32 414 32 1863 32 42 Cuts at: 5898 5930 1373 Size: 32 2626 Fragments arranged by size: 2626 1863 784 572 414 390 135 69 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2730 3124 3579 4527 4773 669 Size: 988 281 792 394 455 948 246 3079 Fragments arranged by size: 3079 988 948 792 455 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7183 HpaI GTT'AAC Cuts at: 3124 3579 4773 3124 Size: 455 1194 5534 Fragments arranged by size: 5534 1194 455 KpnI G_GTAC'C Cuts at: 4508 4508 Size: 7183 MfeI C'AATT_G Cuts at: 2188 4782 2188 Size: 2594 4589 MluI A'CGCG_T Cuts at: 7059 7059 Size: 7183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6297 6481 1305 Size: 4992 184 2007 Fragments arranged by size: 4992 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5361 Fragments arranged by size: 5361 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3168 3318 3705 3860 5141 5500 5659 Size: 648 2002 150 387 155 1281 359 159 Cuts at: 5659 518 Size: 2042 Fragments arranged by size: 2042 2002 1281 648 387 359 159 155 150 MspA1I CmG'CkG Cuts at: 1606 2405 4178 4599 5276 6424 6669 1606 Size: 799 1773 421 677 1148 245 2120 Fragments arranged by size: 2120 1773 1148 799 677 421 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2937 3254 513 Size: 552 939 933 317 4442 Fragments arranged by size: 4442 939 933 552 317 NdeI CA'TA_TG Cuts at: 387 387 Size: 7183 NheI G'CTAG_C Cuts at: 1052 2839 1052 Size: 1787 5396 NotI GC'GGCC_GC Cuts at: 4630 4630 Size: 7183 NspI r_CATG'y Cuts at: 1132 1229 2348 2544 3785 4541 6086 1132 Size: 97 1119 196 1241 756 1545 2229 Fragments arranged by size: 2229 1545 1241 1119 756 196 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7183 PciI A'CATG_T Cuts at: 6082 6082 Size: 7183 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7183 PfoI T'CCnGG_A Cuts at: 4152 4152 Size: 7183 PmeI GTTT'AAAC Cuts at: 4487 4487 Size: 7183 PmlI CAC'GTG Cuts at: 2035 2035 Size: 7183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2063 2095 5252 5284 6765 6797 2063 Size: 32 3157 32 1481 32 2449 Fragments arranged by size: 3157 2449 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 2327 Size: 7183 PshAI GACnn'nnGTC Cuts at: 1613 2428 4178 1613 Size: 815 1750 4618 Fragments arranged by size: 4618 1750 815 PsiI TTA'TAA Cuts at: 4753 4753 Size: 7183 PspOMI G'GGCC_C Cuts at: 2084 2084 Size: 7183 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2853 2885 2853 Size: 32 7151 PstI C_TGCA'G Cuts at: 830 2404 2410 3663 4535 830 Size: 1574 6 1253 872 3478 Fragments arranged by size: 3478 1574 1253 872 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7183 PvuII CAG'CTG Cuts at: 2405 4178 2405 Size: 1773 5410 SacI G_AGCT'C Cuts at: 2081 2081 Size: 7183 SalI G'TCGA_C Cuts at: 1936 4525 1936 Size: 2589 4594 SbfI CC_TGCA'GG Cuts at: 4535 4535 Size: 7183 ScaI AGT'ACT Cuts at: 3586 5431 7143 3586 Size: 1845 1712 3626 Fragments arranged by size: 3626 1845 1712 SexAI A'CCwGG_T Cuts at: 3914 3914 Size: 7183 SfcI C'TryA_G Cuts at: 826 1045 2400 2406 2906 3659 4531 5666 6347 Size: 219 1355 6 500 753 872 1135 681 Cuts at: 6347 6538 826 Size: 191 1471 Fragments arranged by size: 1471 1355 1135 872 753 681 500 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7183 SmaI CCC'GGG Cuts at: 1872 4510 1872 Size: 2638 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5287 6188 6450 820 Size: 197 926 3344 901 262 1553 Fragments arranged by size: 3344 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4974 493 Size: 4481 2702 SpeI A'CTAG_T Cuts at: 152 3581 152 Size: 3429 3754 SphI G_CATG'C Cuts at: 1132 2348 3785 4541 1132 Size: 1216 1437 756 3774 Fragments arranged by size: 3774 1437 1216 756 SspI AAT'ATT Cuts at: 5 52 2535 3714 5107 5 Size: 47 2483 1179 1393 2081 Fragments arranged by size: 2483 2081 1393 1179 47 StuI AGG'CCT Cuts at: 1517 3304 1517 Size: 1787 5396 StyI C'CwwG_G Cuts at: 513 1065 2004 2937 3254 4622 513 Size: 552 939 933 317 1368 3074 Fragments arranged by size: 3074 1368 939 933 552 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4151 5210 5227 5380 5565 6010 325 Size: 1255 2571 1059 17 153 185 445 1498 Fragments arranged by size: 2571 1498 1255 1059 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2384 3584 5429 Size: 274 80 33 51 1443 407 1200 1845 Cuts at: 5429 7141 96 Size: 1712 138 Fragments arranged by size: 1845 1712 1443 1200 407 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4294 4326 7143 7175 4294 Size: 32 2817 32 4302 Fragments arranged by size: 4302 2817 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3185 1316 Size: 1869 5314 XbaI T'CTAG_A Cuts at: 4519 4519 Size: 7183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2095 2095 Size: 7183 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7183 XmaI C'CCGG_G Cuts at: 1870 4508 1870 Size: 2638 4545 XmnI GAAnn'nnTTC Cuts at: 2997 3381 5312 2997 Size: 384 1931 4868 Fragments arranged by size: 4868 1931 384 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4991 276 Size: 53 83 186 936 3457 2468 Fragments arranged by size: 3457 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI