Restriction map for
pFN21AB5777
(Circular) (Six-base) MAPSORT of: pFN21AB5777.seq Check: 49 from: 1 to: 7171
pFN21AB5777 7171 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4981 278
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4514 1937
Size: 2577 4594
Acc65I G'GTAC_C
Cuts at: 4492 4492
Size: 7171
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 5234 6618 6886 1632
Size: 456 3146 1384 268 1917
Fragments arranged by size:
3146 1917 1384 456 268
AflII C'TTAA_G
Cuts at: 820 1017 2461 820
Size: 197 1444 5530
Fragments arranged by size:
5530 1444 197
AflIII A'CryG_T
Cuts at: 1203 6070 7047 1203
Size: 4867 977 1327
Fragments arranged by size:
4867 1327 977
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 2297 5900 2297
Size: 3603 3568
AleI CACnn'nnGTG
Cuts at: 1166 3053 3563 1166
Size: 1887 510 4774
Fragments arranged by size:
4774 1887 510
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3285 3317 3285
Size: 32 7139
AlwNI CAG_nnn'CTG
Cuts at: 3379 4016 4914 6486 3379
Size: 637 898 1572 4064
Fragments arranged by size:
4064 1572 898 637
ApaLI G'TGCA_C
Cuts at: 5228 6384 5228
Size: 1156 6015
ApoI r'AATT_y
Cuts at: 1493 2237 2545 2748 2766 3188 4157 4480 4706
Size: 744 308 203 18 422 969 323 226
Cuts at: 4706 4858 6967 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 969 744 422 323 308 226
203 152 18
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 4496 1870
Size: 73 2553 4545
Fragments arranged by size:
4545 2553 73
BamHI G'GATC_C
Cuts at: 4501 4868 4501
Size: 367 6804
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4492 5947 618
Size: 325 217 627 2705 1455 1842
Fragments arranged by size:
2705 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2396 4418 4490 1391
Size: 1005 2022 72 4072
Fragments arranged by size:
4072 2022 1005 72
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 3011 928
Size: 971 234 878 5088
Fragments arranged by size:
5088 971 878 234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2335 2369 5362 5396 1903
Size: 34 50 34 314 34 2993 34 3678
Fragments arranged by size:
3678 2993 314 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7171
BfrBI ATG'CAT
Cuts at: 3358 3358
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5782 136
Size: 107 122 71 631 605 4110 1525
Fragments arranged by size:
4110 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2413 4429 7166 2413
Size: 2016 2737 2418
Fragments arranged by size:
2737 2418 2016
BlpI GC'TnA_GC
Cuts at: 4417 4588 4417
Size: 171 7000
Bme1580I G_kGCm'C
Cuts at: 1790 2655 5232 6388 1790
Size: 865 2577 1156 2573
Fragments arranged by size:
2577 2573 1156 865
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 4902 5860
Size: 416 53 83 399 24 2765 720 958
Cuts at: 5860 442
Size: 1753
Fragments arranged by size:
2765 1753 958 720 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3665 3993 5831 1239
Size: 2426 328 1838 2579
Fragments arranged by size:
2579 2426 1838 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 3767 5296 6161 6459 2201
Size: 1566 1529 865 298 2913
Fragments arranged by size:
2913 1566 1529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3830 5834 882
Size: 617 2331 2004 2219
Fragments arranged by size:
2331 2219 2004 617
BsaAI yAC'GTr
Cuts at: 493 1204 4962 493
Size: 711 3758 2702
Fragments arranged by size:
3758 2702 711
BsaBI GATnn'nnATC
Cuts at: 2301 2337 7165 2301
Size: 36 4828 2307
Fragments arranged by size:
4828 2307 36
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4978 5360 5995 275
Size: 53 83 186 936 3445 382 635 1451
Fragments arranged by size:
3445 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2037 2046 2106 2690 5604 6002 6276 6423 6834
Size: 9 60 584 2914 398 274 147 411
Cuts at: 6834 2037
Size: 2374
Fragments arranged by size:
2914 2374 584 411 398 274 147 60
9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3991 4021 1178
Size: 30 165 30 2588 30 4328
Fragments arranged by size:
4328 2588 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2386 2585 1186
Size: 1200 199 5772
Fragments arranged by size:
5772 1200 199
BseYI C'CCAG_C
Cuts at: 2179 3982 4050 4315 6374 6870 2179
Size: 1803 68 265 2059 496 2480
Fragments arranged by size:
2480 2059 1803 496 265 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2428 3821 1163
Size: 573 692 1393 4513
Fragments arranged by size:
4513 1393 692 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4621 5382 6410 1540
Size: 275 186 2620 761 1028 2301
Fragments arranged by size:
2620 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4009 5232 5317 6388 4009
Size: 1223 85 1071 4792
Fragments arranged by size:
4792 1223 1071 85
BsmI GAATG_Cn'
Cuts at: 3759 4389 4682 4775 3759
Size: 630 293 93 6155
Fragments arranged by size:
6155 630 293 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2655 4009 4418 4490 5232 5317
Size: 399 606 259 1354 409 72 742 85
Cuts at: 5317 6388 1391
Size: 1071 2174
Fragments arranged by size:
2174 1354 1071 742 606 409 399 259
85 72
BspEI T'CCGG_A
Cuts at: 2037 2046 2690 6834 2037
Size: 9 644 4144 2374
Fragments arranged by size:
4144 2374 644 9
BspHI T'CATG_A
Cuts at: 2310 5060 6790 2310
Size: 2750 1730 2691
Fragments arranged by size:
2750 2691 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2731 4526 844
Size: 1887 1795 3489
Fragments arranged by size:
3489 1887 1795
BsrBI CCG'CTC
Cuts at: 1394 1823 5058 1394
Size: 429 3235 3507
Fragments arranged by size:
3507 3235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 3105 4428 5666 5840 65
Size: 1183 1029 828 1323 1238 174 1396
Fragments arranged by size:
1396 1323 1238 1183 1029 828 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5815 6002 1700
Size: 110 4005 187 2869
Fragments arranged by size:
4005 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3010 3381 96
Size: 2914 371 3886
Fragments arranged by size:
3886 2914 371
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 3570 5231 6243 3570
Size: 1661 1012 4498
Fragments arranged by size:
4498 1661 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2483 2825 3203 4378 794
Size: 1689 342 378 1175 3587
Fragments arranged by size:
3587 1689 1175 378 342
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2413 4429 4501 4868 5253 5270 6722 6808
Size: 441 2016 72 367 385 17 1452 86
Cuts at: 6808 6820 7166 1972
Size: 12 346 1977
Fragments arranged by size:
2016 1977 1452 441 385 367 346 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2566 513
Size: 552 1501 5118
Fragments arranged by size:
5118 1501 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 3585 4687 5481 5501 3585
Size: 1102 794 20 5255
Fragments arranged by size:
5255 1102 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7136
DraI TTT'AAA
Cuts at: 2673 3193 4475 2673
Size: 520 1282 5369
Fragments arranged by size:
5369 1282 520
DraIII CAC_nnn'GTG
Cuts at: 2483 2483
Size: 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3472 4618 5507
Size: 54 1425 50 275 18 1642 1146 889
Cuts at: 5507 7136 8
Size: 1629 43
Fragments arranged by size:
1642 1629 1425 1146 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4618 1537
Size: 275 2806 4090
Fragments arranged by size:
4090 2806 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3477 3483 4097 5101 1353
Size: 2124 6 614 1004 3423
Fragments arranged by size:
3423 2124 1004 614 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5758 6132 6278 387
Size: 1064 138 4169 374 146 1280
Fragments arranged by size:
4169 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 3665 3993 5234 5831 6618 6886
Size: 393 456 1577 328 1241 597 787 268
Cuts at: 6886 1239
Size: 1524
Fragments arranged by size:
1577 1524 1241 787 597 456 393 328
268
EcoRI G'AATT_C
Cuts at: 4157 4480 4858 4157
Size: 323 378 6470
Fragments arranged by size:
6470 378 323
EcoRV GAT'ATC
Cuts at: 4047 4047
Size: 7171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3176 3410 4162 3176
Size: 234 752 6185
Fragments arranged by size:
6185 752 234
FspI TGC'GCA
Cuts at: 2634 5677 2634
Size: 3043 4128
HaeII r_GCGC'y
Cuts at: 1678 3379 6318 1678
Size: 1701 2939 2531
Fragments arranged by size:
2939 2531 1701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2283 2315 2888
Size: 32 135 32 495 32 184 32 573
Cuts at: 2888 2907 2920 2939 3191 3223 5812 5844 5886
Size: 19 13 19 252 32 2589 32 42
Cuts at: 5886 5918 1373
Size: 32 2626
Fragments arranged by size:
2626 2589 573 495 252 184 135 42
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4515 4761 669
Size: 988 281 2577 246 3079
Fragments arranged by size:
3079 2577 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2219 2645 3599 4394 748
Size: 1471 426 954 795 3525
Fragments arranged by size:
3525 1471 954 795 426
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 4496 4496
Size: 7171
MfeI C'AATT_G
Cuts at: 3051 4770 3051
Size: 1719 5452
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2126 2187 2475 3501 3953 4047 4242 6285
Size: 821 61 288 1026 452 94 195 2043
Cuts at: 6285 6469 1305
Size: 184 2007
Fragments arranged by size:
2043 2007 1026 821 452 288 195 184
94 61
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3474 10
Size: 54 1425 343 1642 3707
Fragments arranged by size:
3707 1642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2140 2603 3053 3269 3563 3671 5129
Size: 648 974 463 450 216 294 108 1458
Cuts at: 5129 5488 5647 518
Size: 359 159 2042
Fragments arranged by size:
2042 1458 974 648 463 450 359 294
216 159 108
MspA1I CmG'CkG
Cuts at: 1606 4013 4282 4378 4587 5264 6412 6657 1606
Size: 2407 269 96 209 677 1148 245 2120
Fragments arranged by size:
2407 2120 1148 677 269 245 209 96
NcoI C'CATG_G
Cuts at: 513 1065 2566 513
Size: 552 1501 5118
Fragments arranged by size:
5118 1501 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NsiI A_TGCA'T
Cuts at: 3360 3360
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2179 2629 3362 4529 6074 1132
Size: 97 950 450 733 1167 1545 2229
Fragments arranged by size:
2229 1545 1167 950 733 450 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7171
PciI A'CATG_T
Cuts at: 6070 6070
Size: 7171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3252 3854 1108
Size: 2144 602 4425
Fragments arranged by size:
4425 2144 602
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5240 5272 6753 6785 5240
Size: 32 1481 32 5626
Fragments arranged by size:
5626 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PstI C_TGCA'G
Cuts at: 830 3229 4111 4264 4287 4523 830
Size: 2399 882 153 23 236 3478
Fragments arranged by size:
3478 2399 882 236 153 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 4013 4282 4378 4013
Size: 269 96 6806
Fragments arranged by size:
6806 269 96
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SapI GCTCTTCn'nnn_
Cuts at: 3483 3483
Size: 7171
SbfI CC_TGCA'GG
Cuts at: 4523 4523
Size: 7171
ScaI AGT'ACT
Cuts at: 5419 7131 5419
Size: 1712 5459
SfcI C'TryA_G
Cuts at: 826 1045 2586 3225 4107 4260 4283 4519 5654
Size: 219 1541 639 882 153 23 236 1135
Cuts at: 5654 6335 6526 826
Size: 681 191 1471
Fragments arranged by size:
1541 1471 1135 882 681 639 236 219
191 153 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 4498 1872
Size: 2626 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2461 3746 5275 6176 6438
Size: 197 926 273 245 1285 1529 901 262
Cuts at: 6438 820
Size: 1553
Fragments arranged by size:
1553 1529 1285 926 901 273 262 245
197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 2843 152
Size: 2691 4480
SphI G_CATG'C
Cuts at: 1132 3362 4529 1132
Size: 2230 1167 3774
Fragments arranged by size:
3774 2230 1167
SspI AAT'ATT
Cuts at: 5 52 2974 5095 5
Size: 47 2922 2121 2081
Fragments arranged by size:
2922 2121 2081 47
StuI AGG'CCT
Cuts at: 1517 4214 1517
Size: 2697 4474
StyI C'CwwG_G
Cuts at: 513 1065 2566 4610 513
Size: 552 1501 2044 3074
Fragments arranged by size:
3074 2044 1501 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5198 5215 5368 5553 5998 325
Size: 1255 3618 17 153 185 445 1498
Fragments arranged by size:
3618 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3010 3031 3381
Size: 274 80 33 51 1443 1033 21 350
Cuts at: 3381 3620 5417 7129 96
Size: 239 1797 1712 138
Fragments arranged by size:
1797 1712 1443 1033 350 274 239 138
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7131 7163 7131
Size: 32 7139
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7171
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2938 3886 2938
Size: 948 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 4496 1870
Size: 2626 4545
XmnI GAAnn'nnTTC
Cuts at: 5300 5300
Size: 7171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4979 276
Size: 53 83 186 936 3445 2468
Fragments arranged by size:
3445 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI