Restriction map for
pFN21AB5776
(Circular) (Six-base) MAPSORT of: pFN21AB5776.seq Check: 1715 from: 1 to: 7003
pFN21AB5776 7003 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4813 278
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3331 3417 4346 1937
Size: 1394 86 929 4594
Fragments arranged by size:
4594 1394 929 86
Acc65I G'GTAC_C
Cuts at: 2509 4324 2509
Size: 1815 5188
AclI AA'CG_TT
Cuts at: 1563 2726 5130 5503 1563
Size: 1163 2404 373 3063
Fragments arranged by size:
3063 2404 1163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5066 6450 6718 1632
Size: 3434 1384 268 1917
Fragments arranged by size:
3434 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6806
AflIII A'CryG_T
Cuts at: 1203 2792 3532 5902 6879 1203
Size: 1589 740 2370 977 1327
Fragments arranged by size:
2370 1589 1327 977 740
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 2432 2839 4182 1166
Size: 1266 407 1343 3987
Fragments arranged by size:
3987 1343 1266 407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7003
AlwNI CAG_nnn'CTG
Cuts at: 2056 2933 4746 6318 2056
Size: 877 1813 1572 2741
Fragments arranged by size:
2741 1813 1572 877
ApaI G_GGCC'C
Cuts at: 2988 2988
Size: 7003
ApaLI G'TGCA_C
Cuts at: 5060 6216 5060
Size: 1156 5847
ApoI r'AATT_y
Cuts at: 1493 2204 2601 2804 2991 3644 4131 4173 4312
Size: 711 397 203 187 653 487 42 139
Cuts at: 4312 4538 4690 6799 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 711 653 487 397 226 203
187 152 139 42
AseI AT'TA_AT
Cuts at: 160 1032 4079 5557 160
Size: 872 3047 1478 1606
Fragments arranged by size:
3047 1606 1478 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 3340 4328 1870
Size: 73 135 1262 988 4545
Fragments arranged by size:
4545 1262 988 135 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2493 2526 2493
Size: 33 6970
BamHI G'GATC_C
Cuts at: 3848 4333 4700 3848
Size: 485 367 6151
Fragments arranged by size:
6151 485 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2093 2099 2509 3865
Size: 325 217 627 300 6 6 410 1356
Cuts at: 3865 4324 5779 618
Size: 459 1455 1842
Fragments arranged by size:
1842 1455 1356 627 459 410 325 300
217 6 6
BanII G_rGCy'C
Cuts at: 1391 2079 2988 3608 4322 1391
Size: 688 909 620 714 4072
Fragments arranged by size:
4072 909 714 688 620
BbeI G_GCGC'C
Cuts at: 2091 2097 2103 2091
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2473 3309 3768 928
Size: 971 574 836 459 4163
Fragments arranged by size:
4163 971 836 574 459
BbvCI CC'TCA_GC
Cuts at: 3270 3480 3270
Size: 210 6793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2952 2986 5194 5228 1903
Size: 34 1015 34 2208 34 3678
Fragments arranged by size:
3678 2208 1015 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 4013 4895 6105 2350
Size: 1663 882 1210 3248
Fragments arranged by size:
3248 1663 1210 882
BclI T'GATC_A
Cuts at: 1548 3654 1548
Size: 2106 4897
BfrBI ATG'CAT
Cuts at: 3926 3926
Size: 7003
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2114 5614
Size: 107 122 71 631 605 337 105 3500
Cuts at: 5614 136
Size: 1525
Fragments arranged by size:
3500 1525 631 605 337 122 107 105
71
BglII A'GATC_T
Cuts at: 4033 6998 4033
Size: 2965 4038
BlpI GC'TnA_GC
Cuts at: 2015 4420 2015
Size: 2405 4598
Bme1580I G_kGCm'C
Cuts at: 1790 2988 5064 6220 1790
Size: 1198 2076 1156 2573
Fragments arranged by size:
2573 2076 1198 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7003
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3586 4734 5692
Size: 416 53 83 399 24 2169 1148 958
Cuts at: 5692 442
Size: 1753
Fragments arranged by size:
2169 1753 1148 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2679 3246 3592 4014 5663 1239
Size: 1022 418 567 346 422 1649 2579
Fragments arranged by size:
2579 1649 1022 567 422 418 346
Bpu10I CC'TnA_GC
Cuts at: 2737 2923 3270 3480 2737
Size: 186 347 210 6260
Fragments arranged by size:
6260 347 210 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3719 5128 5993 6291 3719
Size: 1409 865 298 4431
Fragments arranged by size:
4431 1409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3078 3150 3632 3723 4108 5666 882
Size: 617 1579 72 482 91 385 1558 2219
Fragments arranged by size:
2219 1579 1558 617 482 385 91 72
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 3057 3094 3400 3894 6997 3057
Size: 37 306 494 3103 3063
Fragments arranged by size:
3103 3063 494 306 37
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2094 2100 2918
Size: 53 83 186 936 555 6 6 818
Cuts at: 2918 4810 5192 5827 275
Size: 1892 382 635 1451
Fragments arranged by size:
1892 1451 936 818 635 555 382 186
83 53 6 6
BsaWI w'CCGG_w
Cuts at: 2134 5436 5834 6108 6255 6666 2134
Size: 3302 398 274 147 411 2471
Fragments arranged by size:
3302 2471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2517 2547 4042
Size: 30 165 30 726 30 358 30 1495
Cuts at: 4042 4072 1178
Size: 30 4109
Fragments arranged by size:
4109 1495 726 358 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 1186
Size: 967 6036
BseYI C'CCAG_C
Cuts at: 6206 6702 6206
Size: 496 6507
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3669 1163
Size: 573 1933 4497
Fragments arranged by size:
4497 1933 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4453 5214 6242 1540
Size: 275 186 2452 761 1028 2301
Fragments arranged by size:
2452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5064 5149 6220 5064
Size: 85 1071 5847
Fragments arranged by size:
5847 1071 85
BsmI GAATG_Cn'
Cuts at: 3888 4514 4607 3888
Size: 626 93 6284
Fragments arranged by size:
6284 626 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3012 3012
Size: 7003
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2988 3608 4322 5064 5149 6220
Size: 399 289 909 620 714 742 85 1071
Cuts at: 6220 1391
Size: 2174
Fragments arranged by size:
2174 1071 909 742 714 620 399 289
85
BspEI T'CCGG_A
Cuts at: 2134 6666 2134
Size: 4532 2471
BspHI T'CATG_A
Cuts at: 3757 4892 6622 3757
Size: 1135 1730 4138
Fragments arranged by size:
4138 1730 1135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2521 4358 844
Size: 1677 1837 3489
Fragments arranged by size:
3489 1837 1677
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 4161 4890 1394
Size: 429 196 2142 729 3507
Fragments arranged by size:
3507 2142 729 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 5498 5672 65
Size: 1183 2915 1335 174 1396
Fragments arranged by size:
2915 1396 1335 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2090 2096 5647 5834 1700
Size: 110 274 6 6 3551 187 2869
Fragments arranged by size:
3551 2869 274 187 110 6 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 5063 6075 5063
Size: 1012 5991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2933 794
Size: 2139 4864
BstBI TT'CG_AA
Cuts at: 5807 5807
Size: 7003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7003
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2631 2434
Size: 197 6806
BstYI r'GATC_y
Cuts at: 1972 3395 3848 4033 4333 4700 5085 5102 6554
Size: 1423 453 185 300 367 385 17 1452
Cuts at: 6554 6640 6652 6998 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1423 453 385 367 346 300
185 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 513
Size: 552 939 99 5413
Fragments arranged by size:
5413 939 552 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6264
Fragments arranged by size:
6264 683 56
BtsI GCAGTG_nn'
Cuts at: 3453 4189 4519 5313 5333 3453
Size: 736 330 794 20 5123
Fragments arranged by size:
5123 794 736 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2191 2226 529
Size: 35 1627 35 5306
Fragments arranged by size:
5306 1627 35 35
DraI TTT'AAA
Cuts at: 4307 4307
Size: 7003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6010 809
Size: 507 4694 1802
Fragments arranged by size:
4694 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4450 5339 6968
Size: 54 1425 50 275 18 2620 889 1629
Cuts at: 6968 8
Size: 43
Fragments arranged by size:
2620 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4450 1537
Size: 275 2638 4090
Fragments arranged by size:
4090 2638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2116 2383 2907 3554 4067 4933 1353
Size: 763 267 524 647 513 866 3423
Fragments arranged by size:
3423 866 763 647 524 513 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5590 5964 6110 387
Size: 1064 138 4001 374 146 1280
Fragments arranged by size:
4001 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2679 3246 3592 4014 5066 5663
Size: 393 629 418 567 346 422 1052 597
Cuts at: 5663 6450 6718 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1052 787 629 597 567 422 418
393 346 268
EcoO109I rG'GnC_Cy
Cuts at: 2191 2985 3266 3822 2191
Size: 794 281 556 5372
Fragments arranged by size:
5372 794 556 281
EcoRI G'AATT_C
Cuts at: 2601 4312 4690 2601
Size: 1711 378 4914
Fragments arranged by size:
4914 1711 378
FspI TGC'GCA
Cuts at: 5509 5509
Size: 7003
HaeII r_GCGC'y
Cuts at: 1678 2015 2091 2097 2103 6150 1678
Size: 337 76 6 6 4047 2531
Fragments arranged by size:
4047 2531 337 76 6 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2517 2549 2554 2559 2586
Size: 32 135 32 945 32 5 5 27
Cuts at: 2586 2591 3077 3109 3167 3199 4042 4074 5644
Size: 5 486 32 58 32 843 32 1570
Cuts at: 5644 5676 5718 5750 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1570 945 843 486 135 58 42
32 32 32 32 32 32 32 32
27 5 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2172 4347 4593 669
Size: 988 281 234 2175 246 3079
Fragments arranged by size:
3079 2175 988 281 246 234
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7003
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KasI G'GCGC_C
Cuts at: 2087 2093 2099 2087
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
KpnI G_GTAC'C
Cuts at: 2513 4328 2513
Size: 1815 5188
MfeI C'AATT_G
Cuts at: 2625 4602 2625
Size: 1977 5026
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2305 2664 3067 6117 6301 1305
Size: 1000 359 403 3050 184 2007
Fragments arranged by size:
3050 2007 1000 403 359 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5181
Fragments arranged by size:
5181 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2839 3438 3881 4182 4961 5320
Size: 648 1266 407 599 443 301 779 359
Cuts at: 5320 5479 518
Size: 159 2042
Fragments arranged by size:
2042 1266 779 648 599 443 407 359
301 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2784 4419 5096 6244 6489 1606
Size: 499 679 1635 677 1148 245 2120
Fragments arranged by size:
2120 1635 1148 679 677 499 245
NaeI GCC'GGC
Cuts at: 2086 2092 2098 2086
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
NarI GG'CG_CC
Cuts at: 2088 2094 2100 2088
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5512
Fragments arranged by size:
5512 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NgoMIV G'CCGG_C
Cuts at: 2084 2090 2096 2084
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NsiI A_TGCA'T
Cuts at: 3928 3928
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 2675 2796 3536 4361 5906 1132
Size: 97 1446 121 740 825 1545 2229
Fragments arranged by size:
2229 1545 1446 825 740 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7003
PciI A'CATG_T
Cuts at: 2792 3532 5902 2792
Size: 740 2370 3893
Fragments arranged by size:
3893 2370 740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3858 1108
Size: 2750 4253
PfoI T'CCnGG_A
Cuts at: 3262 3262
Size: 7003
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5072 5104 6585 6617 5072
Size: 32 1481 32 5458
Fragments arranged by size:
5458 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3266 3822 3266
Size: 556 6447
PshAI GACnn'nnGTC
Cuts at: 1613 2754 1613
Size: 1141 5862
PsiI TTA'TAA
Cuts at: 2802 4573 2802
Size: 1771 5232
PspOMI G'GGCC_C
Cuts at: 2984 2984
Size: 7003
PspXI vC'TCGA_Gb
Cuts at: 3340 3340
Size: 7003
PstI C_TGCA'G
Cuts at: 830 4355 830
Size: 3525 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
SacII CC_GC'GG
Cuts at: 2106 2106
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SanDI GG'GwC_CC
Cuts at: 3822 3822
Size: 7003
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 3516 5251 6963 3516
Size: 1735 1712 3556
Fragments arranged by size:
3556 1735 1712
SfcI C'TryA_G
Cuts at: 826 1045 3798 4351 5486 6167 6358 826
Size: 219 2753 553 1135 681 191 1471
Fragments arranged by size:
2753 1471 1135 681 553 219 191
SfoI GGC'GCC
Cuts at: 2089 2095 2101 2089
Size: 6 6 6991
Fragments arranged by size:
6991 6 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 2090 2096 1700
Size: 390 6 6607
Fragments arranged by size:
6607 390 6
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3340 3734 5107 6008 6270 820
Size: 197 926 1397 394 1373 901 262 1553
Fragments arranged by size:
1553 1397 1373 926 901 394 262 197
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 4361 1132
Size: 3229 3774
SspI AAT'ATT
Cuts at: 5 52 3139 4927 5
Size: 47 3087 1788 2081
Fragments arranged by size:
3087 2081 1788 47
StuI AGG'CCT
Cuts at: 1517 2246 1517
Size: 729 6274
StyI C'CwwG_G
Cuts at: 513 1065 2004 3160 4442 513
Size: 552 939 1156 1282 3074
Fragments arranged by size:
3074 1282 1156 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2913 3102 4279 5030 5047 5200 5385
Size: 1255 1333 189 1177 751 17 153 185
Cuts at: 5385 5830 325
Size: 445 1498
Fragments arranged by size:
1498 1333 1255 1177 751 445 189 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2822 3514 5249
Size: 274 80 33 51 1443 845 692 1735
Cuts at: 5249 6961 96
Size: 1712 138
Fragments arranged by size:
1735 1712 1443 845 692 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2129 2161 3024 3056 6963 6995 2129
Size: 32 863 32 3907 32 2137
Fragments arranged by size:
3907 2137 863 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7003
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3167 3859 3167
Size: 692 6311
XhoI C'TCGA_G
Cuts at: 1943 3340 1943
Size: 1397 5606
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 5132 5132
Size: 7003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4811 276
Size: 53 83 186 936 3277 2468
Fragments arranged by size:
3277 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI PacI PmlI PsrI PvuII RsrII SacI SapI
SexAI SfiI SrfI SwaI