Restriction map for
pFN21AB5775
(Circular) (Six-base) MAPSORT of: pFN21AB5775.seq Check: 5805 from: 1 to: 7144
pFN21AB5775 7144 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4954 278
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4487 1937
Size: 2550 4594
Acc65I G'GTAC_C
Cuts at: 4465 4465
Size: 7144
AclI AA'CG_TT
Cuts at: 1563 5271 5644 1563
Size: 3708 373 3063
Fragments arranged by size:
3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2303 2345 2373 3945 5207 6591 6859
Size: 645 26 42 28 1572 1262 1384 268
Cuts at: 6859 1632
Size: 1917
Fragments arranged by size:
1917 1572 1384 1262 645 268 42 28
26
AflII C'TTAA_G
Cuts at: 820 1017 2240 3746 820
Size: 197 1223 1506 4218
Fragments arranged by size:
4218 1506 1223 197
AflIII A'CryG_T
Cuts at: 1203 3446 6043 7020 1203
Size: 2243 2597 977 1327
Fragments arranged by size:
2597 2243 1327 977
AgeI A'CCGG_T
Cuts at: 5975 5975
Size: 7144
AhdI GACnn_n'nnGTC
Cuts at: 5873 5873
Size: 7144
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7144
AlwNI CAG_nnn'CTG
Cuts at: 2035 4887 6459 2035
Size: 2852 1572 2720
Fragments arranged by size:
2852 2720 1572
ApaLI G'TGCA_C
Cuts at: 5201 6357 5201
Size: 1156 5988
ApoI r'AATT_y
Cuts at: 1493 2600 3226 3509 4431 4453 4679 4831 6940
Size: 1107 626 283 922 22 226 152 2109
Cuts at: 6940 1493
Size: 1697
Fragments arranged by size:
2109 1697 1107 922 626 283 226 152
22
AseI AT'TA_AT
Cuts at: 160 1032 5698 160
Size: 872 4666 1606
Fragments arranged by size:
4666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 4469 1870
Size: 73 207 2319 4545
Fragments arranged by size:
4545 2319 207 73
BamHI G'GATC_C
Cuts at: 4474 4841 4474
Size: 367 6777
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2199 2833 4465 5920
Size: 325 217 627 359 53 634 1632 1455
Cuts at: 5920 618
Size: 1842
Fragments arranged by size:
1842 1632 1455 634 627 359 325 217
53
BanII G_rGCy'C
Cuts at: 1391 2136 4463 1391
Size: 745 2327 4072
Fragments arranged by size:
4072 2327 745
BbeI G_GCGC'C
Cuts at: 2150 2203 2150
Size: 53 7091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4012 4148 928
Size: 971 2113 136 3924
Fragments arranged by size:
3924 2113 971 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5335 5369 1903
Size: 34 3398 34 3678
Fragments arranged by size:
3678 3398 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5036 6246 5036
Size: 1210 5934
BclI T'GATC_A
Cuts at: 1548 3807 1548
Size: 2259 4885
BfrBI ATG'CAT
Cuts at: 4123 4325 4123
Size: 202 6942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 5755 136
Size: 107 122 71 631 605 406 3677 1525
Fragments arranged by size:
3677 1525 631 605 406 122 107 71
BglII A'GATC_T
Cuts at: 7139 7139
Size: 7144
BlpI GC'TnA_GC
Cuts at: 2126 4561 2126
Size: 2435 4709
Bme1580I G_kGCm'C
Cuts at: 1790 5205 6361 1790
Size: 3415 1156 2573
Fragments arranged by size:
3415 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4875 5833 442
Size: 416 53 83 399 24 3458 958 1753
Fragments arranged by size:
3458 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2185 2246 2270 4302 5804 1239
Size: 946 61 24 2032 1502 2579
Fragments arranged by size:
2579 2032 1502 946 61 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5269 6134 6432 5269
Size: 865 298 5981
Fragments arranged by size:
5981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 5807 882
Size: 617 1308 3000 2219
Fragments arranged by size:
3000 2219 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 4935 493
Size: 711 2243 1488 2702
Fragments arranged by size:
2702 2243 1488 711
BsaBI GATnn'nnATC
Cuts at: 3894 7138 3894
Size: 3244 3900
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2147 2200 2667 4951
Size: 53 83 186 936 614 53 467 2284
Cuts at: 4951 5333 5968 275
Size: 382 635 1451
Fragments arranged by size:
2284 1451 936 635 614 467 382 186
83 53 53
BsaWI w'CCGG_w
Cuts at: 3838 5577 5975 6249 6396 6807 3838
Size: 1739 398 274 147 411 4175
Fragments arranged by size:
4175 1739 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6919
Fragments arranged by size:
6919 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 4221 1186
Size: 996 2039 4109
Fragments arranged by size:
4109 2039 996
BseYI C'CCAG_C
Cuts at: 2267 6347 6843 2267
Size: 4080 496 2568
Fragments arranged by size:
4080 2568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6571
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2082 4594 5355 6383 1540
Size: 275 186 48 33 2512 761 1028 2301
Fragments arranged by size:
2512 2301 1028 761 275 186 48 33
BsiHKAI G_wGCw'C
Cuts at: 2136 3039 3649 5205 5290 6361 2136
Size: 903 610 1556 85 1071 2919
Fragments arranged by size:
2919 1556 1071 903 610 85
BsmI GAATG_Cn'
Cuts at: 3478 4655 4748 3478
Size: 1177 93 5874
Fragments arranged by size:
5874 1177 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2674 2674
Size: 7144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 3039 3649 4463 5205 5290 6361
Size: 399 346 903 610 814 742 85 1071
Cuts at: 6361 1391
Size: 2174
Fragments arranged by size:
2174 1071 903 814 742 610 399 346
85
BspEI T'CCGG_A
Cuts at: 6807 6807
Size: 7144
BspHI T'CATG_A
Cuts at: 3721 3804 5033 6763 3721
Size: 83 1229 1730 4102
Fragments arranged by size:
4102 1730 1229 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4499 844
Size: 3655 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5031 1394
Size: 429 3208 3507
Fragments arranged by size:
3507 3208 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3967 4104 4226 4303 5639 5813 65
Size: 1183 2719 137 122 77 1336 174 1396
Fragments arranged by size:
2719 1396 1336 1183 174 137 122 77
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 5788 5975 1700
Size: 110 271 3707 187 2869
Fragments arranged by size:
3707 2869 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 3295 96
Size: 3199 3945
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7144
BssSI C'ACGA_G
Cuts at: 5204 6216 5204
Size: 1012 6132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7144
BstBI TT'CG_AA
Cuts at: 3622 5948 3622
Size: 2326 4818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7144
BstXI CCAn_nnnn'nTGG
Cuts at: 3482 3482
Size: 7144
BstYI r'GATC_y
Cuts at: 1972 3124 3955 4474 4841 5226 5243 6695 6781
Size: 1152 831 519 367 385 17 1452 86
Cuts at: 6781 6793 7139 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1152 831 519 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4201 4201
Size: 7144
BtgI C'CryG_G
Cuts at: 513 1065 2055 4243 513
Size: 552 990 2188 3414
Fragments arranged by size:
3414 2188 990 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6405
Fragments arranged by size:
6405 683 56
BtsI GCAGTG_nn'
Cuts at: 2237 2547 3992 4660 5454 5474 2237
Size: 310 1445 668 794 20 3907
Fragments arranged by size:
3907 1445 794 668 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2462 2497 529
Size: 35 1898 35 5176
Fragments arranged by size:
5176 1898 35 35
DraI TTT'AAA
Cuts at: 3516 4448 3516
Size: 932 6212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6151 809
Size: 507 4835 1802
Fragments arranged by size:
4835 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2079 4591
Size: 54 1425 50 275 18 216 33 2512
Cuts at: 4591 5480 7109 8
Size: 889 1629 43
Fragments arranged by size:
2512 1629 1425 889 275 216 54 50
43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2079 4591 1537
Size: 275 234 33 2512 4090
Fragments arranged by size:
4090 2512 275 234 33
EarI CTCTTCn'nnn_
Cuts at: 1353 3635 5074 1353
Size: 2282 1439 3423
Fragments arranged by size:
3423 2282 1439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 2195 5731 6105 6251 387
Size: 1064 138 603 3 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 603 374 146 138 3
EcoICRI GAG'CTC
Cuts at: 2134 2134
Size: 7144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2185 2246 2270 2277 2303 2345 2373
Size: 393 553 61 24 7 26 42 28
Cuts at: 2373 3945 4302 5207 5804 6591 6859 1239
Size: 1572 357 905 597 787 268 1524
Fragments arranged by size:
1572 1524 905 787 597 553 393 357
268 61 42 28 26 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2141
Size: 7144
EcoO109I rG'GnC_Cy
Cuts at: 2020 2032 2020
Size: 12 7132
EcoRI G'AATT_C
Cuts at: 2600 4453 4831 2600
Size: 1853 378 4913
Fragments arranged by size:
4913 1853 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 4161 4165 2434
Size: 1727 4 5413
Fragments arranged by size:
5413 1727 4
FspI TGC'GCA
Cuts at: 5650 5650
Size: 7144
HaeII r_GCGC'y
Cuts at: 1678 2150 2203 6291 1678
Size: 472 53 4088 2531
Fragments arranged by size:
4088 2531 472 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2654 2686 5785 5817 5859
Size: 32 135 32 1082 32 3099 32 42
Cuts at: 5859 5891 1373
Size: 32 2626
Fragments arranged by size:
3099 2626 1082 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4488 4734 669
Size: 988 281 2550 246 3079
Fragments arranged by size:
3079 2550 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3462 748
Size: 2714 4430
HpaI GTT'AAC
Cuts at: 4734 4734
Size: 7144
KasI G'GCGC_C
Cuts at: 2146 2199 2146
Size: 53 7091
KpnI G_GTAC'C
Cuts at: 4469 4469
Size: 7144
MfeI C'AATT_G
Cuts at: 4743 4743
Size: 7144
MluI A'CGCG_T
Cuts at: 7020 7020
Size: 7144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 3102 6258 6442 1305
Size: 1432 365 3156 184 2007
Fragments arranged by size:
3156 2007 1432 365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5322
Fragments arranged by size:
5322 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3219 5102 5461 5620 518
Size: 648 2053 1883 359 159 2042
Fragments arranged by size:
2053 2042 1883 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2113 2620 3097 3788 4560 5237 6385
Size: 459 48 507 477 691 772 677 1148
Cuts at: 6385 6630 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 772 691 677 507 477 459
245 48
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 7144
NarI GG'CG_CC
Cuts at: 2147 2200 2147
Size: 53 7091
NcoI C'CATG_G
Cuts at: 513 1065 4243 513
Size: 552 3178 3414
Fragments arranged by size:
3414 3178 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7144
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 7144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7144
NotI GC'GGCC_GC
Cuts at: 4591 4591
Size: 7144
NsiI A_TGCA'T
Cuts at: 4125 4327 4125
Size: 202 6942
NspI r_CATG'y
Cuts at: 1132 1229 2549 4123 4502 6047 1132
Size: 97 1320 1574 379 1545 2229
Fragments arranged by size:
2229 1574 1545 1320 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7144
PciI A'CATG_T
Cuts at: 6043 6043
Size: 7144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 4244 1108
Size: 1878 1258 4008
Fragments arranged by size:
4008 1878 1258
PfoI T'CCnGG_A
Cuts at: 2136 2136
Size: 7144
PmeI GTTT'AAAC
Cuts at: 4448 4448
Size: 7144
PmlI CAC'GTG
Cuts at: 3447 3447
Size: 7144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5213 5245 6726 6758 5213
Size: 32 1481 32 5599
Fragments arranged by size:
5599 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2020
Size: 7144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7144
PsiI TTA'TAA
Cuts at: 4714 4714
Size: 7144
PstI C_TGCA'G
Cuts at: 830 2337 4195 4496 830
Size: 1507 1858 301 3478
Fragments arranged by size:
3478 1858 1507 301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7144
PvuII CAG'CTG
Cuts at: 2620 3097 3788 2620
Size: 477 691 5976
Fragments arranged by size:
5976 691 477
SacI G_AGCT'C
Cuts at: 2136 2136
Size: 7144
SalI G'TCGA_C
Cuts at: 1936 4486 1936
Size: 2550 4594
SanDI GG'GwC_CC
Cuts at: 2020 2020
Size: 7144
SbfI CC_TGCA'GG
Cuts at: 2337 4496 2337
Size: 2159 4985
ScaI AGT'ACT
Cuts at: 4046 5392 7104 4046
Size: 1346 1712 4086
Fragments arranged by size:
4086 1712 1346
SfcI C'TryA_G
Cuts at: 826 1045 2027 2333 3704 4065 4191 4492 5627
Size: 219 982 306 1371 361 126 301 1135
Cuts at: 5627 6308 6499 826
Size: 681 191 1471
Fragments arranged by size:
1471 1371 1135 982 681 361 306 301
219 191 126
SfoI GGC'GCC
Cuts at: 2148 2201 2148
Size: 53 7091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7144
SmaI CCC'GGG
Cuts at: 1872 4471 1872
Size: 2599 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 3746 5248 6149 6411 820
Size: 197 926 297 1506 1502 901 262 1553
Fragments arranged by size:
1553 1506 1502 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 4935 493
Size: 4442 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7144
SphI G_CATG'C
Cuts at: 1132 4502 1132
Size: 3370 3774
SspI AAT'ATT
Cuts at: 5 52 3427 3677 5068 5
Size: 47 3375 250 1391 2081
Fragments arranged by size:
3375 2081 1391 250 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7144
StyI C'CwwG_G
Cuts at: 513 1065 2857 3714 4243 4583 513
Size: 552 1792 857 529 340 3074
Fragments arranged by size:
3074 1792 857 552 529 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 5171 5188 5341 5526 5971 325
Size: 1255 702 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 702 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3295 4044 5390
Size: 274 80 33 51 1443 1318 749 1346
Cuts at: 5390 7102 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 1318 749 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 3327 3359 7104 7136 2418
Size: 32 877 32 3745 32 2426
Fragments arranged by size:
3745 2426 877 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7144
XbaI T'CTAG_A
Cuts at: 3613 3886 4480 3613
Size: 273 594 6277
Fragments arranged by size:
6277 594 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3397 4262 2011
Size: 1386 865 4893
Fragments arranged by size:
4893 1386 865
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7144
XmaI C'CCGG_G
Cuts at: 1870 4469 1870
Size: 2599 4545
XmnI GAAnn'nnTTC
Cuts at: 2358 3239 5273 2358
Size: 881 2034 4229
Fragments arranged by size:
4229 2034 881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4952 276
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI
NruI PacI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SrfI SwaI