Restriction map for
pFN21AB5773
(Circular) (Six-base) MAPSORT of: pFN21AB5773.seq Check: 5126 from: 1 to: 7171
pFN21AB5773 7171 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2826 4981 278
Size: 53 83 186 936 1290 2155 2468
Fragments arranged by size:
2468 2155 1290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2080 4514 1937
Size: 143 2434 4594
Fragments arranged by size:
4594 2434 143
Acc65I G'GTAC_C
Cuts at: 3384 4492 3384
Size: 1108 6063
AclI AA'CG_TT
Cuts at: 1563 5298 5671 1563
Size: 3735 373 3063
Fragments arranged by size:
3735 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2693 3857 4115 4249 4408 5234 6618
Size: 806 255 1164 258 134 159 826 1384
Cuts at: 6618 6886 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1164 826 806 268 258 255
159 134
AfeI AGC'GCT
Cuts at: 3114 3114
Size: 7171
AflII C'TTAA_G
Cuts at: 820 1017 3284 820
Size: 197 2267 4707
Fragments arranged by size:
4707 2267 197
AflIII A'CryG_T
Cuts at: 1203 2154 4230 6070 7047 1203
Size: 951 2076 1840 977 1327
Fragments arranged by size:
2076 1840 1327 977 951
AgeI A'CCGG_T
Cuts at: 6002 6002
Size: 7171
AhdI GACnn_n'nnGTC
Cuts at: 5900 5900
Size: 7171
AleI CACnn'nnGTG
Cuts at: 1166 3761 1166
Size: 2595 4576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2729 3593 1184
Size: 1545 864 4762
Fragments arranged by size:
4762 1545 864
AlwNI CAG_nnn'CTG
Cuts at: 2371 2614 2647 2759 3064 3103 4010 4444 4914
Size: 243 33 112 305 39 907 434 470
Cuts at: 4914 6486 2371
Size: 1572 3056
Fragments arranged by size:
3056 1572 907 470 434 305 243 112
39 33
ApaI G_GGCC'C
Cuts at: 2298 3182 3964 2298
Size: 884 782 5505
Fragments arranged by size:
5505 884 782
ApaLI G'TGCA_C
Cuts at: 3428 5228 6384 3428
Size: 1800 1156 4215
Fragments arranged by size:
4215 1800 1156
ApoI r'AATT_y
Cuts at: 1493 4480 4706 4858 6967 1493
Size: 2987 226 152 2109 1697
Fragments arranged by size:
2987 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5725 160
Size: 872 4693 1606
Fragments arranged by size:
4693 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
AvaI C'yCGr_G
Cuts at: 1870 1943 3045 3400 4119 4496 1870
Size: 73 1102 355 719 377 4545
Fragments arranged by size:
4545 1102 719 377 355 73
BamHI G'GATC_C
Cuts at: 3831 4501 4868 3831
Size: 670 367 6134
Fragments arranged by size:
6134 670 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3384 4492 5947 618
Size: 325 217 627 1597 1108 1455 1842
Fragments arranged by size:
1842 1597 1455 1108 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 2385 2437 2892 3018 3182 3192 3964
Size: 907 87 52 455 126 164 10 772
Cuts at: 3964 4490 1391
Size: 526 4072
Fragments arranged by size:
4072 907 772 526 455 164 126 87
52 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3983 928
Size: 971 2084 4116
Fragments arranged by size:
4116 2084 971
BbvCI CC'TCA_GC
Cuts at: 4295 4295
Size: 7171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2321 2355 5362 5396 1903
Size: 34 384 34 3007 34 3678
Fragments arranged by size:
3678 3007 384 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5063 6273 5063
Size: 1210 5961
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7171
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 5782 136
Size: 107 122 71 631 605 365 3745 1525
Fragments arranged by size:
3745 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 7166 7166
Size: 7171
BlpI GC'TnA_GC
Cuts at: 2309 3417 4588 2309
Size: 1108 1171 4892
Fragments arranged by size:
4892 1171 1108
Bme1580I G_kGCm'C
Cuts at: 1790 2298 2455 3182 3432 3654 3964 5232 6388
Size: 508 157 727 250 222 310 1268 1156
Cuts at: 6388 1790
Size: 2573
Fragments arranged by size:
2573 1268 1156 727 508 310 250 222
157
BmgBI CAC'GTC
Cuts at: 1318 3231 4231 1318
Size: 1913 1000 4258
Fragments arranged by size:
4258 1913 1000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4902 5860 442
Size: 416 53 83 399 24 3485 958 1753
Fragments arranged by size:
3485 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3806
Size: 7171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2717 2876 2897 3188 3296 4046 5831
Size: 866 612 159 21 291 108 750 1785
Cuts at: 5831 1239
Size: 2579
Fragments arranged by size:
2579 1785 866 750 612 291 159 108
21
Bpu10I CC'TnA_GC
Cuts at: 2252 2300 4295 2252
Size: 48 1995 5128
Fragments arranged by size:
5128 1995 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3788 5296 6161 6459 2879
Size: 909 1508 865 298 3591
Fragments arranged by size:
3591 1508 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 5834 882
Size: 617 2754 1581 2219
Fragments arranged by size:
2754 2219 1581 617
BsaAI yAC'GTr
Cuts at: 493 1204 3478 4962 493
Size: 711 2274 1484 2702
Fragments arranged by size:
2702 2274 1484 711
BsaBI GATnn'nnATC
Cuts at: 7165 7165
Size: 7171
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2823 3739 4978 5360
Size: 53 83 186 936 1290 916 1239 382
Cuts at: 5360 5995 275
Size: 635 1451
Fragments arranged by size:
1451 1290 1239 936 916 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3018 3349 5604 6002 6276 6423 6834 3018
Size: 331 2255 398 274 147 411 3355
Fragments arranged by size:
3355 2255 411 398 331 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3000 3030 3209 3239 4019
Size: 30 165 30 1597 30 179 30 780
Cuts at: 4019 4049 1178
Size: 30 4300
Fragments arranged by size:
4300 1597 780 179 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 2876 3044 4207 1186
Size: 1483 207 168 1163 4150
Fragments arranged by size:
4150 1483 1163 207 168
BseYI C'CCAG_C
Cuts at: 2109 2566 3064 3141 3603 4392 4418 6374 6870
Size: 457 498 77 462 789 26 1956 496
Cuts at: 6870 2109
Size: 2410
Fragments arranged by size:
2410 1956 789 498 496 462 457 77
26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 2633 2735 2942 3077 3139 3377
Size: 573 311 586 102 207 135 62 238
Cuts at: 3377 3623 3865 3941 1163
Size: 246 242 76 4393
Fragments arranged by size:
4393 586 573 311 246 242 238 207
135 102 76 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4210 4621 5382 6410 1540
Size: 275 186 2209 411 761 1028 2301
Fragments arranged by size:
2301 2209 1028 761 411 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2437 2892 3018 3174 3432 3602 5232 5317
Size: 52 455 126 156 258 170 1630 85
Cuts at: 5317 6388 2385
Size: 1071 3168
Fragments arranged by size:
3168 1630 1071 455 258 170 156 126
85 52
BsmI GAATG_Cn'
Cuts at: 3570 4682 4775 3570
Size: 1112 93 5966
Fragments arranged by size:
5966 1112 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3779 3872 3779
Size: 93 7078
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2385 2437 2455 2892 3018 3174
Size: 399 508 87 52 18 437 126 156
Cuts at: 3174 3182 3192 3432 3602 3654 3964 4490 5232
Size: 8 10 240 170 52 310 526 742
Cuts at: 5232 5317 6388 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 508 437 399 310
240 170 156 126 87 85 52 52
18 10 8
BspEI T'CCGG_A
Cuts at: 3018 3349 6834 3018
Size: 331 3485 3355
Fragments arranged by size:
3485 3355 331
BspHI T'CATG_A
Cuts at: 5060 6790 5060
Size: 1730 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 3097 4526 844
Size: 1811 442 1429 3489
Fragments arranged by size:
3489 1811 1429 442
BsrBI CCG'CTC
Cuts at: 1394 1823 3052 5058 1394
Size: 429 1229 2006 3507
Fragments arranged by size:
3507 2006 1229 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5666 5840 65
Size: 1183 4418 174 1396
Fragments arranged by size:
4418 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5815 6002 1700
Size: 110 4005 187 2869
Fragments arranged by size:
4005 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7171
BssSI C'ACGA_G
Cuts at: 2651 5231 6243 2651
Size: 2580 1012 3579
Fragments arranged by size:
3579 2580 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 2371 2647 2759 3082 3103 3151 4038
Size: 1265 312 276 112 323 21 48 887
Cuts at: 4038 794
Size: 3927
Fragments arranged by size:
3927 1265 887 323 312 276 112 48
21
BstBI TT'CG_AA
Cuts at: 5975 5975
Size: 7171
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7171
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 7171
BstYI r'GATC_y
Cuts at: 1972 2281 2679 3279 3831 4501 4868 5253 5270
Size: 309 398 600 552 670 367 385 17
Cuts at: 5270 6722 6808 6820 7166 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 670 600 552 398 385 367
346 309 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2775 4150 4191 2775
Size: 1375 41 5755
Fragments arranged by size:
5755 1375 41
BtgI C'CryG_G
Cuts at: 513 1065 2004 2041 3388 513
Size: 552 939 37 1347 4296
Fragments arranged by size:
4296 1347 939 552 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6432
Fragments arranged by size:
6432 683 56
BtsI GCAGTG_nn'
Cuts at: 2366 2636 2852 3092 4394 4687 5481 5501 2366
Size: 270 216 240 1302 293 794 20 4036
Fragments arranged by size:
4036 1302 794 293 270 240 216 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3607 3642 529
Size: 35 3043 35 4058
Fragments arranged by size:
4058 3043 35 35
DraI TTT'AAA
Cuts at: 4475 4475
Size: 7171
DraIII CAC_nnn'GTG
Cuts at: 2834 2834
Size: 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6178 809
Size: 507 4862 1802
Fragments arranged by size:
4862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2730 2961 4207
Size: 54 1425 50 275 18 900 231 1246
Cuts at: 4207 4618 5507 7136 8
Size: 411 889 1629 43
Fragments arranged by size:
1629 1425 1246 900 889 411 275 231
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4207 4618 1537
Size: 275 2395 411 4090
Fragments arranged by size:
4090 2395 411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 2414 3021 3617 5101 1353
Size: 667 394 607 596 1484 3423
Fragments arranged by size:
3423 1484 667 607 596 394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3299 5758 6132 6278 387
Size: 1064 138 1710 2459 374 146 1280
Fragments arranged by size:
2459 1710 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2383 2435 2890 3016 2383
Size: 52 455 126 6538
Fragments arranged by size:
6538 455 126 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2438 2693 2717 2876 2897 3188
Size: 393 473 333 255 24 159 21 291
Cuts at: 3188 3296 3857 4046 4115 4249 4408 5234 5831
Size: 108 561 189 69 134 159 826 597
Cuts at: 5831 6618 6886 1239
Size: 787 268 1524
Fragments arranged by size:
1524 826 787 597 561 473 393 333
291 268 255 189 159 159 134 108
69 24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2714 2651
Size: 63 7108
EcoO109I rG'GnC_Cy
Cuts at: 2047 2248 2294 2295 2644 3179 3682 3960 4051
Size: 201 46 1 349 535 503 278 91
Cuts at: 4051 4156 4195 4275 2047
Size: 105 39 80 4943
Fragments arranged by size:
4943 535 503 349 278 201 105 91
80 46 39 1
EcoRI G'AATT_C
Cuts at: 4480 4858 4480
Size: 378 6793
FspI TGC'GCA
Cuts at: 2525 2583 5677 2525
Size: 58 3094 4019
Fragments arranged by size:
4019 3094 58
HaeII r_GCGC'y
Cuts at: 1678 3116 6318 1678
Size: 1438 3202 2531
Fragments arranged by size:
3202 2531 1438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 3209 3241 5812
Size: 32 135 32 701 32 904 32 2571
Cuts at: 5812 5844 5886 5918 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2571 904 701 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4515 4761 669
Size: 988 281 2577 246 3079
Fragments arranged by size:
3079 2577 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7171
HpaI GTT'AAC
Cuts at: 4761 4761
Size: 7171
KpnI G_GTAC'C
Cuts at: 3388 4496 3388
Size: 1108 6063
MfeI C'AATT_G
Cuts at: 4770 4770
Size: 7171
MluI A'CGCG_T
Cuts at: 7047 7047
Size: 7171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6285 6469 1305
Size: 4980 184 2007
Fragments arranged by size:
4980 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 2963 10
Size: 54 1425 343 900 231 4218
Fragments arranged by size:
4218 1425 900 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3761 3908 5129 5488 5647 518
Size: 648 1803 792 147 1221 359 159 2042
Fragments arranged by size:
2042 1803 1221 792 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2113 2371 2756 2875 2896 3004 3061 3337
Size: 507 258 385 119 21 108 57 276
Cuts at: 3337 3379 3735 4007 4414 4587 5264 6412 6657
Size: 42 356 272 407 173 677 1148 245
Cuts at: 6657 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 507 407 385 356 276
272 258 245 173 119 108 57 42
21
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5680
Fragments arranged by size:
5680 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7171
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7171
NotI GC'GGCC_GC
Cuts at: 4618 4618
Size: 7171
NspI r_CATG'y
Cuts at: 1132 1229 2158 4529 6074 1132
Size: 97 929 2371 1545 2229
Fragments arranged by size:
2371 2229 1545 929 97
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 6484
PciI A'CATG_T
Cuts at: 2154 6070 2154
Size: 3916 3255
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3529 1108
Size: 2421 4750
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 7171
PmeI GTTT'AAAC
Cuts at: 4475 4475
Size: 7171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 5240 5272 6753 6785 2960
Size: 32 2248 32 1481 32 3346
Fragments arranged by size:
3346 2248 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2644 3682 4051 2047
Size: 597 1038 369 5167
Fragments arranged by size:
5167 1038 597 369
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7171
PsiI TTA'TAA
Cuts at: 4741 4741
Size: 7171
PspOMI G'GGCC_C
Cuts at: 2294 3178 3960 2294
Size: 884 782 5505
Fragments arranged by size:
5505 884 782
PstI C_TGCA'G
Cuts at: 830 2055 2166 2376 2643 2688 2755 3099 3108
Size: 1225 111 210 267 45 67 344 9
Cuts at: 3108 3138 3159 3213 3342 3990 4449 4523 830
Size: 30 21 54 129 648 459 74 3478
Fragments arranged by size:
3478 1225 648 459 344 267 210 129
111 74 67 54 45 30 21 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7171
PvuII CAG'CTG
Cuts at: 2371 2756 2896 3004 3061 3379 4007 2371
Size: 385 140 108 57 318 628 5535
Fragments arranged by size:
5535 628 385 318 140 108 57
SacI G_AGCT'C
Cuts at: 2385 2437 2892 3018 2385
Size: 52 455 126 6538
Fragments arranged by size:
6538 455 126 52
SalI G'TCGA_C
Cuts at: 1936 4513 1936
Size: 2577 4594
SapI GCTCTTCn'nnn_
Cuts at: 2414 2414
Size: 7171
SbfI CC_TGCA'GG
Cuts at: 2166 2688 3213 4523 2166
Size: 522 525 1310 4814
Fragments arranged by size:
4814 1310 525 522
ScaI AGT'ACT
Cuts at: 5419 7131 5419
Size: 1712 5459
SexAI A'CCwGG_T
Cuts at: 3684 3684
Size: 7171
SfcI C'TryA_G
Cuts at: 826 1045 2051 2162 2350 2372 2639 2684 2751
Size: 219 1006 111 188 22 267 45 67
Cuts at: 2751 3095 3104 3134 3155 3209 3338 3818 3986
Size: 344 9 30 21 54 129 480 168
Cuts at: 3986 4445 4519 5654 6335 6526 826
Size: 459 74 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 480 459 344 267
219 191 188 168 129 111 74 67
54 45 30 22 21 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7171
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7171
SmaI CCC'GGG
Cuts at: 1872 3047 4498 1872
Size: 1175 1451 4545
Fragments arranged by size:
4545 1451 1175
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3284 3803 5275 6176 6438
Size: 197 926 915 426 519 1472 901 262
Cuts at: 6438 820
Size: 1553
Fragments arranged by size:
1553 1472 926 915 901 519 426 262
197
SnaBI TAC'GTA
Cuts at: 493 4962 493
Size: 4469 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7171
SphI G_CATG'C
Cuts at: 1132 4529 1132
Size: 3397 3774
SspI AAT'ATT
Cuts at: 5 52 5095 5
Size: 47 5043 2081
Fragments arranged by size:
5043 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7171
StyI C'CwwG_G
Cuts at: 513 1065 2004 2508 2788 3528 3654 4610 513
Size: 552 939 504 280 740 126 956 3074
Fragments arranged by size:
3074 956 939 740 552 504 280 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3053 3283 3617 3655 3683 3899 5198
Size: 1255 1473 230 334 38 28 216 1299
Cuts at: 5198 5215 5368 5553 5998 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1473 1299 1255 445 334 230 216
185 153 38 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3843 5417 7129
Size: 274 80 33 51 1443 1866 1574 1712
Cuts at: 7129 96
Size: 138
Fragments arranged by size:
1866 1712 1574 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2808 2840 4019 4051 7131 7163 2141
Size: 32 635 32 1179 32 3080 32 2149
Fragments arranged by size:
3080 2149 1179 635 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7171
XbaI T'CTAG_A
Cuts at: 4507 4507
Size: 7171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7171
XmaI C'CCGG_G
Cuts at: 1870 3045 4496 1870
Size: 1175 1451 4545
Fragments arranged by size:
4545 1451 1175
XmnI GAAnn'nnTTC
Cuts at: 3561 5300 3561
Size: 1739 5432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2824 4979 276
Size: 53 83 186 936 1290 2155 2468
Fragments arranged by size:
2468 2155 1290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI