Restriction map for
pFN21AB5769
(Circular) (Six-base) MAPSORT of: pFN21AB5769.seq Check: 9461 from: 1 to: 6955
pFN21AB5769 6955 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 6955
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4765 278
Size: 53 83 186 936 3229 2468
Fragments arranged by size:
3229 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4298 1937
Size: 2361 4594
Acc65I G'GTAC_C
Cuts at: 4062 4276 4062
Size: 214 6741
AclI AA'CG_TT
Cuts at: 1563 5082 5455 1563
Size: 3519 373 3063
Fragments arranged by size:
3519 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2483 2813 2818 3926 5018 6402 6670
Size: 531 320 330 5 1108 1092 1384 268
Cuts at: 6670 1632
Size: 1917
Fragments arranged by size:
1917 1384 1108 1092 531 330 320 268
5
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6758
AflIII A'CryG_T
Cuts at: 1203 5854 6831 1203
Size: 4651 977 1327
Fragments arranged by size:
4651 1327 977
AgeI A'CCGG_T
Cuts at: 5786 5786
Size: 6955
AhdI GACnn_n'nnGTC
Cuts at: 2515 5684 2515
Size: 3169 3786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3227 1184
Size: 2043 4912
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 6923
AlwNI CAG_nnn'CTG
Cuts at: 2627 2650 2671 2791 2953 3937 4698 6270 2627
Size: 23 21 120 162 984 761 1572 3312
Fragments arranged by size:
3312 1572 984 761 162 120 23 21
ApaI G_GGCC'C
Cuts at: 2057 3044 3516 2057
Size: 987 472 5496
Fragments arranged by size:
5496 987 472
ApaLI G'TGCA_C
Cuts at: 5012 6168 5012
Size: 1156 5799
ApoI r'AATT_y
Cuts at: 1493 2704 4264 4490 4642 6751 1493
Size: 1211 1560 226 152 2109 1697
Fragments arranged by size:
2109 1697 1560 1211 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4022 5509 160
Size: 872 2990 1487 1606
Fragments arranged by size:
2990 1606 1487 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6955
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 4280 1870
Size: 73 127 2210 4545
Fragments arranged by size:
4545 2210 127 73
AvrII C'CTAG_G
Cuts at: 2044 2044
Size: 6955
BamHI G'GATC_C
Cuts at: 2040 2075 4285 4652 2040
Size: 35 2210 367 4343
Fragments arranged by size:
4343 2210 367 35
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3282 3521 3667 3940 4062
Size: 325 217 627 1495 239 146 273 122
Cuts at: 4062 4276 5731 618
Size: 214 1455 1842
Fragments arranged by size:
1842 1495 1455 627 325 273 239 217
214 146 122
BanII G_rGCy'C
Cuts at: 1391 2057 2380 2453 2568 3044 3410 3503 3516
Size: 666 323 73 115 476 366 93 13
Cuts at: 3516 3904 4274 1391
Size: 388 370 4072
Fragments arranged by size:
4072 666 476 388 370 366 323 115
93 73 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 928
Size: 971 615 5369
Fragments arranged by size:
5369 971 615
BbvCI CC'TCA_GC
Cuts at: 2888 3485 2888
Size: 597 6358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2915 2949 5146 5180 1903
Size: 34 50 34 894 34 2197 34 3678
Fragments arranged by size:
3678 2197 894 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4847 6057 4847
Size: 1210 5745
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6955
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2927 5566 136
Size: 107 122 71 631 605 1255 2639 1525
Fragments arranged by size:
2639 1525 1255 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2837 6950 2837
Size: 4113 2842
BlpI GC'TnA_GC
Cuts at: 3425 4372 3425
Size: 947 6008
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2092 3044 3516 3672 5016 6172 1790
Size: 267 35 952 472 156 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 952 472 267 156 35
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6955
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 3046 4686
Size: 416 53 83 399 24 704 925 1640
Cuts at: 4686 5644 442
Size: 958 1753
Fragments arranged by size:
1753 1640 958 925 704 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2388 1056
Size: 1332 5623
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3360 3360
Size: 6955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2901 3410 4091 5615 1239
Size: 1127 535 509 681 1524 2579
Fragments arranged by size:
2579 1524 1127 681 535 509
Bpu10I CC'TnA_GC
Cuts at: 2379 2888 3026 3485 2379
Size: 509 138 459 5849
Fragments arranged by size:
5849 509 459 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5080 5945 6243 5080
Size: 865 298 5792
Fragments arranged by size:
5792 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3565 3606 5618 882
Size: 617 2066 41 2012 2219
Fragments arranged by size:
2219 2066 2012 617 41
BsaAI yAC'GTr
Cuts at: 493 1204 4746 493
Size: 711 3542 2702
Fragments arranged by size:
3542 2702 711
BsaBI GATnn'nnATC
Cuts at: 4107 6949 4107
Size: 2842 4113
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2200 4762 5144 5779
Size: 53 83 186 936 667 2562 382 635
Cuts at: 5779 275
Size: 1451
Fragments arranged by size:
2562 1451 936 667 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3896 5388 5786 6060 6207 6618 3896
Size: 1492 398 274 147 411 4233
Fragments arranged by size:
4233 1492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 2753 2783 3449
Size: 30 165 30 936 30 384 30 666
Cuts at: 3449 3479 3651 3681 4050 4080 1178
Size: 30 172 30 369 30 4053
Fragments arranged by size:
4053 936 666 384 369 172 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2058 2363 2391 3100 3464 1186
Size: 872 305 28 709 364 4677
Fragments arranged by size:
4677 872 709 364 305 28
BseYI C'CCAG_C
Cuts at: 2050 2079 2239 2453 2547 3117 3671 3930 4183
Size: 29 160 214 94 570 554 259 253
Cuts at: 4183 6158 6654 2050
Size: 1975 496 2351
Fragments arranged by size:
2351 1975 570 554 496 259 253 214
160 94 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3197 1163
Size: 573 1461 4921
Fragments arranged by size:
4921 1461 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3351 3355 4405 5166 6194
Size: 275 186 1183 167 4 1050 761 1028
Cuts at: 6194 1540
Size: 2301
Fragments arranged by size:
2301 1183 1050 1028 761 275 186 167
4
BsiHKAI G_wGCw'C
Cuts at: 3503 5016 5101 6172 3503
Size: 1513 85 1071 4286
Fragments arranged by size:
4286 1513 1071 85
BsmI GAATG_Cn'
Cuts at: 4466 4559 4466
Size: 93 6862
BsmBI CGTCTCn'nnnn_
Cuts at: 2750 2750
Size: 6955
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2092 2380 2453 2568 3044 3410
Size: 399 267 35 288 73 115 476 366
Cuts at: 3410 3503 3516 3672 3904 4274 5016 5101 6172
Size: 93 13 156 232 370 742 85 1071
Cuts at: 6172 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 476 399 370 366 288
267 232 156 115 93 85 73 35
13
BspEI T'CCGG_A
Cuts at: 6618 6618
Size: 6955
BspHI T'CATG_A
Cuts at: 4844 6574 4844
Size: 1730 5225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 3165 4310 844
Size: 1275 1046 1145 3489
Fragments arranged by size:
3489 1275 1145 1046
BsrBI CCG'CTC
Cuts at: 1394 1823 3310 3598 3744 4842 1394
Size: 429 1487 288 146 1098 3507
Fragments arranged by size:
3507 1487 1098 429 288 146
BsrDI GCAATG_nn'
Cuts at: 65 1248 3690 5450 5624 65
Size: 1183 2442 1760 174 1396
Fragments arranged by size:
2442 1760 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 3135 3350 5599 5786 1700
Size: 110 206 1119 215 2249 187 2869
Fragments arranged by size:
2869 2249 1119 215 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6955
BssHII G'CGCG_C
Cuts at: 1925 3288 1925
Size: 1363 5592
BssSI C'ACGA_G
Cuts at: 3236 3260 5015 6027 3236
Size: 24 1755 1012 4164
Fragments arranged by size:
4164 1755 1012 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4193 794
Size: 3399 3556
BstBI TT'CG_AA
Cuts at: 2167 5759 2167
Size: 3592 3363
BstEII G'GTnAC_C
Cuts at: 1184 3799 3892 1184
Size: 2615 93 4247
Fragments arranged by size:
4247 2615 93
BstXI CCAn_nnnn'nTGG
Cuts at: 2034 3368 2034
Size: 1334 5621
BstYI r'GATC_y
Cuts at: 1972 2040 2075 2492 2837 4285 4652 5037 5054
Size: 68 35 417 345 1448 367 385 17
Cuts at: 5054 6506 6592 6604 6950 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1448 417 385 367 346 345
86 68 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2820 3112 2820
Size: 292 6663
BtgI C'CryG_G
Cuts at: 513 1065 2772 3417 513
Size: 552 1707 645 4051
Fragments arranged by size:
4051 1707 645 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3547 487
Size: 683 56 2321 3895
Fragments arranged by size:
3895 2321 683 56
BtsI GCAGTG_nn'
Cuts at: 3762 3947 4004 4471 5265 5285 3762
Size: 185 57 467 794 20 5432
Fragments arranged by size:
5432 794 467 185 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6955
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6920
DraI TTT'AAA
Cuts at: 4259 4259
Size: 6955
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3846 5962 809
Size: 507 2530 2116 1802
Fragments arranged by size:
2530 2116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2122 2299
Size: 54 1425 50 275 18 194 98 177
Cuts at: 2299 3181 3232 3352 4160 4402 5291 6920 8
Size: 882 51 120 808 242 889 1629 43
Fragments arranged by size:
1629 1425 889 882 808 275 242 194
177 120 98 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 3352 4402 1537
Size: 275 1369 171 1050 4090
Fragments arranged by size:
4090 1369 1050 275 171
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 3441 3563 4253 4885 1353
Size: 1436 652 122 690 632 3423
Fragments arranged by size:
3423 1436 690 652 632 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5542 5916 6062 387
Size: 1064 138 3953 374 146 1280
Fragments arranged by size:
3953 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3501 3501
Size: 6955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2366 2483 2813 2818 2901 3410
Size: 393 531 203 117 330 5 83 509
Cuts at: 3410 3926 4091 5018 5615 6402 6670 1239
Size: 516 165 927 597 787 268 1524
Fragments arranged by size:
1524 927 787 597 531 516 509 393
330 268 203 165 117 83 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 6955
EcoO109I rG'GnC_Cy
Cuts at: 2054 2362 2543 2624 2850 2929 3040 3512 2054
Size: 308 181 81 226 79 111 472 5497
Fragments arranged by size:
5497 472 308 226 181 111 81 79
EcoRI G'AATT_C
Cuts at: 4264 4642 4264
Size: 378 6577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3790 3790
Size: 6955
FspI TGC'GCA
Cuts at: 3542 5461 3542
Size: 1919 5036
HaeII r_GCGC'y
Cuts at: 1678 3107 3432 6102 1678
Size: 1429 325 2670 2531
Fragments arranged by size:
2670 2531 1429 325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2339 2371 2501
Size: 32 135 32 495 32 240 32 130
Cuts at: 2501 2533 2666 2698 3358 3390 3449 3481 5596
Size: 32 133 32 660 32 59 32 2115
Cuts at: 5596 5628 5670 5702 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2115 660 495 240 135 133 130
59 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 4299 4545 669
Size: 988 281 1122 1239 246 3079
Fragments arranged by size:
3079 1239 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3767 3908 748
Size: 3019 141 3795
Fragments arranged by size:
3795 3019 141
HpaI GTT'AAC
Cuts at: 3060 4545 3060
Size: 1485 5470
KpnI G_GTAC'C
Cuts at: 4066 4280 4066
Size: 214 6741
MfeI C'AATT_G
Cuts at: 2303 4554 2303
Size: 2251 4704
MluI A'CGCG_T
Cuts at: 6831 6831
Size: 6955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 2306 3234 3371 3435 3615 6069 6253
Size: 900 101 928 137 64 180 2454 184
Cuts at: 6253 1305
Size: 2007
Fragments arranged by size:
2454 2007 928 900 184 180 137 101
64
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2026 2124 3234 10
Size: 54 1425 343 194 98 1110 3731
Fragments arranged by size:
3731 1425 1110 343 194 98 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3887 3966 4913 5272 5431 518
Size: 648 2721 79 947 359 159 2042
Fragments arranged by size:
2721 2042 947 648 359 159 79
MspA1I CmG'CkG
Cuts at: 1606 2601 3128 3419 3475 3652 4213 4222 4371
Size: 995 527 291 56 177 561 9 149
Cuts at: 4371 5048 6196 6441 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 995 677 561 527 291 245
177 149 56 9
NaeI GCC'GGC
Cuts at: 3137 3137
Size: 6955
NcoI C'CATG_G
Cuts at: 513 1065 2772 513
Size: 552 1707 4696
Fragments arranged by size:
4696 1707 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6955
NgoMIV G'CCGG_C
Cuts at: 3135 3135
Size: 6955
NheI G'CTAG_C
Cuts at: 1052 2384 1052
Size: 1332 5623
NotI GC'GGCC_GC
Cuts at: 4402 4402
Size: 6955
NruI TCG'CGA
Cuts at: 3566 3566
Size: 6955
NspI r_CATG'y
Cuts at: 1132 1229 2634 4313 5858 1132
Size: 97 1405 1679 1545 2229
Fragments arranged by size:
2229 1679 1545 1405 97
PasI CC'CwG_GG
Cuts at: 1348 4205 1348
Size: 2857 4098
PciI A'CATG_T
Cuts at: 5854 5854
Size: 6955
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 2971 3937 4129 1108
Size: 911 952 966 192 3934
Fragments arranged by size:
3934 966 952 911 192
PfoI T'CCnGG_A
Cuts at: 2472 2726 2952 3918 4068 2472
Size: 254 226 966 150 5359
Fragments arranged by size:
5359 966 254 226 150
PmeI GTTT'AAAC
Cuts at: 4259 4259
Size: 6955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 5024 5056 6537 6569 2753
Size: 32 2239 32 1481 32 3139
Fragments arranged by size:
3139 2239 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2850 2850
Size: 6955
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6955
PsiI TTA'TAA
Cuts at: 4525 4525
Size: 6955
PspOMI G'GGCC_C
Cuts at: 2053 3040 3512 2053
Size: 987 472 5496
Fragments arranged by size:
5496 987 472
PstI C_TGCA'G
Cuts at: 830 2646 3252 3594 4307 830
Size: 1816 606 342 713 3478
Fragments arranged by size:
3478 1816 713 606 342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6955
PvuII CAG'CTG
Cuts at: 4213 4222 4213
Size: 9 6946
SacI G_AGCT'C
Cuts at: 3503 3503
Size: 6955
SacII CC_GC'GG
Cuts at: 3420 3420
Size: 6955
SalI G'TCGA_C
Cuts at: 1936 4297 1936
Size: 2361 4594
SanDI GG'GwC_CC
Cuts at: 2850 2850
Size: 6955
SbfI CC_TGCA'GG
Cuts at: 3594 4307 3594
Size: 713 6242
ScaI AGT'ACT
Cuts at: 3868 5203 6915 3868
Size: 1335 1712 3908
Fragments arranged by size:
3908 1712 1335
SexAI A'CCwGG_T
Cuts at: 2583 3615 2583
Size: 1032 5923
SfcI C'TryA_G
Cuts at: 826 1045 2642 3248 3590 4303 5438 6119 6310
Size: 219 1597 606 342 713 1135 681 191
Cuts at: 6310 826
Size: 1471
Fragments arranged by size:
1597 1471 1135 713 681 606 342 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6955
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6955
SmaI CCC'GGG
Cuts at: 1872 4282 1872
Size: 2410 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5059 5960 6222 820
Size: 197 926 3116 901 262 1553
Fragments arranged by size:
3116 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4746 493
Size: 4253 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6955
SphI G_CATG'C
Cuts at: 1132 2634 4313 1132
Size: 1502 1679 3774
Fragments arranged by size:
3774 1679 1502
SspI AAT'ATT
Cuts at: 5 52 4879 5
Size: 47 4827 2081
Fragments arranged by size:
4827 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6955
StyI C'CwwG_G
Cuts at: 513 1065 2044 2772 3010 3361 4394 513
Size: 552 979 728 238 351 1033 3074
Fragments arranged by size:
3074 1033 979 728 552 351 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2598 3040 3313 4982 4999 5152 5337
Size: 1255 1018 442 273 1669 17 153 185
Cuts at: 5337 5782 325
Size: 445 1498
Fragments arranged by size:
1669 1498 1255 1018 445 442 273 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3866 5201
Size: 274 80 33 51 1443 597 1292 1335
Cuts at: 5201 6913 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1335 1292 597 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3651 3683 6915 6947 3651
Size: 32 3232 32 3659
Fragments arranged by size:
3659 3232 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6955
XbaI T'CTAG_A
Cuts at: 3715 4291 3715
Size: 576 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6955
XmaI C'CCGG_G
Cuts at: 1870 4280 1870
Size: 2410 4545
XmnI GAAnn'nnTTC
Cuts at: 2468 5084 2468
Size: 2616 4339
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4763 276
Size: 53 83 186 936 3229 2468
Fragments arranged by size:
3229 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NarI NsiI PacI PmlI
PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI
XcmI