Restriction map for
pFN21AB5766
(Circular) (Six-base) MAPSORT of: pFN21AB5766.seq Check: 2029 from: 1 to: 6946
pFN21AB5766 6946 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2417 2527 2417
Size: 110 6836
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4289 1937
Size: 2352 4594
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2400 2589 3669 4013 4130 4166 5009
Size: 462 306 189 1080 344 117 36 843
Cuts at: 5009 6393 6661 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1080 843 462 344 306 268
189 117 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 5845 6822 1203
Size: 4642 977 1327
Fragments arranged by size:
4642 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 3342 4232 1166
Size: 2176 890 3880
Fragments arranged by size:
3880 2176 890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2703 1184
Size: 1519 5427
AlwNI CAG_nnn'CTG
Cuts at: 2453 3508 3647 3967 3991 4689 6261 2453
Size: 1055 139 320 24 698 1572 3138
Fragments arranged by size:
3138 1572 1055 698 320 139 24
ApaLI G'TGCA_C
Cuts at: 5003 6159 5003
Size: 1156 5790
ApoI r'AATT_y
Cuts at: 1493 3494 3791 4255 4481 4633 6742 1493
Size: 2001 297 464 226 152 2109 1697
Fragments arranged by size:
2109 2001 1697 464 297 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 3066 4271 1870
Size: 73 1123 1205 4545
Fragments arranged by size:
4545 1205 1123 73
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4267 5722 618
Size: 325 217 627 2480 1455 1842
Fragments arranged by size:
2480 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3871 4011 4265 1391
Size: 2480 140 254 4072
Fragments arranged by size:
4072 2480 254 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3719 928
Size: 971 1820 4155
Fragments arranged by size:
4155 1820 971
BbvCI CC'TCA_GC
Cuts at: 3984 3984
Size: 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5137 5171 1903
Size: 34 50 34 3116 34 3678
Fragments arranged by size:
3678 3116 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 2485 3208 3445 1548
Size: 937 723 237 5049
Fragments arranged by size:
5049 937 723 237
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5557 136
Size: 107 122 71 631 605 3885 1525
Fragments arranged by size:
3885 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6941 6941
Size: 6946
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 5007 6163 1790
Size: 3217 1156 2573
Fragments arranged by size:
3217 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4677 5635 442
Size: 416 53 83 399 24 3260 958 1753
Fragments arranged by size:
3260 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4088 1056
Size: 3032 3914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2394 3087 3807 4100 5606 1239
Size: 927 228 693 720 293 1506 2579
Fragments arranged by size:
2579 1506 927 720 693 293 228
Bpu10I CC'TnA_GC
Cuts at: 3027 3984 3027
Size: 957 5989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5071 5936 6234 5071
Size: 865 298 5783
Fragments arranged by size:
5783 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3851 5609 882
Size: 617 2352 1758 2219
Fragments arranged by size:
2352 2219 1758 617
BsaAI yAC'GTr
Cuts at: 493 1204 4737 493
Size: 711 3533 2702
Fragments arranged by size:
3533 2702 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4753 5135 5770 275
Size: 53 83 186 936 3220 382 635 1451
Fragments arranged by size:
3220 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5379 5777 6051 6198 6609 5379
Size: 398 274 147 411 5716
Fragments arranged by size:
5716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3935 3965 1178
Size: 30 165 30 2532 30 4159
Fragments arranged by size:
4159 2532 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6946
BseYI C'CCAG_C
Cuts at: 2446 2924 3414 3745 6149 6645 2446
Size: 478 490 331 2404 496 2747
Fragments arranged by size:
2747 2404 496 490 478 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 4256 1163
Size: 573 769 1751 3853
Fragments arranged by size:
3853 1751 769 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 5007 5092 6163 4011
Size: 996 85 1071 4794
Fragments arranged by size:
4794 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2704 4457 4550 2704
Size: 1753 93 5100
Fragments arranged by size:
5100 1753 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3554 3554
Size: 6946
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3871 4011 4265 5007 5092 6163 1391
Size: 399 2081 140 254 742 85 1071 2174
Fragments arranged by size:
2174 2081 1071 742 399 254 140 85
BspEI T'CCGG_A
Cuts at: 6609 6609
Size: 6946
BspHI T'CATG_A
Cuts at: 3046 4835 6565 3046
Size: 1789 1730 3427
Fragments arranged by size:
3427 1789 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 2527 4301 844
Size: 1573 110 1774 3489
Fragments arranged by size:
3489 1774 1573 110
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2081 2297 3183 3915 5441 5615 65
Size: 1183 833 216 886 732 1526 174 1396
Fragments arranged by size:
1526 1396 1183 886 833 732 216 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5590 5777 1700
Size: 110 3780 187 2869
Fragments arranged by size:
3780 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3846 96
Size: 3750 3196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6946
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstXI CCAn_nnnn'nTGG
Cuts at: 4129 4129
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941
Size: 2304 367 385 17 1452 86 12 346
Cuts at: 6941 1972
Size: 1977
Fragments arranged by size:
2304 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2466 3464 4017 2466
Size: 998 553 5395
Fragments arranged by size:
5395 998 553
BtgI C'CryG_G
Cuts at: 513 1065 2748 3393 3838 513
Size: 552 1683 645 445 3621
Fragments arranged by size:
3621 1683 645 552 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6207
Fragments arranged by size:
6207 683 56
BtsI GCAGTG_nn'
Cuts at: 4462 5256 5276 4462
Size: 794 20 6132
Fragments arranged by size:
6132 794 20
ClaI AT'CG_AT
Cuts at: 1554 2312 1554
Size: 758 6188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6911
DraI TTT'AAA
Cuts at: 2478 3079 4036 4250 2478
Size: 601 957 214 5174
Fragments arranged by size:
5174 957 601 214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5953 809
Size: 507 4637 1802
Fragments arranged by size:
4637 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4393 5282 6911
Size: 54 1425 50 275 18 2563 889 1629
Cuts at: 6911 8
Size: 43
Fragments arranged by size:
2563 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 2865 3645 3849 4876 1353
Size: 911 601 780 204 1027 3423
Fragments arranged by size:
3423 1027 911 780 601 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4009 4009
Size: 6946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2166 2394 2400 2589 3087 3669
Size: 393 462 72 228 6 189 498 582
Cuts at: 3669 3807 4013 4100 4130 4166 5009 5606 6393
Size: 138 206 87 30 36 843 597 787
Cuts at: 6393 6661 1239
Size: 268 1524
Fragments arranged by size:
1524 843 787 597 582 498 462 393
268 228 206 189 138 87 72 36
30 6
EcoRI G'AATT_C
Cuts at: 3791 4255 4633 3791
Size: 464 378 6104
Fragments arranged by size:
6104 464 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3878 3878
Size: 6946
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 4212 6093 1678
Size: 2534 1881 2531
Fragments arranged by size:
2534 2531 1881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2423 2455 2765 2797 2965
Size: 32 135 32 851 32 310 32 168
Cuts at: 2965 2997 3143 3161 3175 3193 5587 5619 5661
Size: 32 146 18 14 18 2394 32 42
Cuts at: 5661 5693 1373
Size: 32 2626
Fragments arranged by size:
2626 2394 851 310 168 146 135 42
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2127 3568 4290 4536 669
Size: 988 281 189 1441 722 246 3079
Fragments arranged by size:
3079 1441 988 722 281 246 189
HindIII A'AGCT_T
Cuts at: 748 3801 748
Size: 3053 3893
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 6060 6244 1305
Size: 1449 3306 184 2007
Fragments arranged by size:
3306 2007 1449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5124
Fragments arranged by size:
5124 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3342 4232 4904 5263 5422 518
Size: 648 2176 890 672 359 159 2042
Fragments arranged by size:
2176 2042 890 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3214 3434 3745 4108 4362 5039 6187 6432
Size: 1608 220 311 363 254 677 1148 245
Cuts at: 6432 1606
Size: 2120
Fragments arranged by size:
2120 1608 1148 677 363 311 254 245
220
NcoI C'CATG_G
Cuts at: 513 1065 2748 3393 513
Size: 552 1683 645 4066
Fragments arranged by size:
4066 1683 645 552
NdeI CA'TA_TG
Cuts at: 387 4048 4201 387
Size: 3661 153 3132
Fragments arranged by size:
3661 3132 153
NheI G'CTAG_C
Cuts at: 1052 4084 1052
Size: 3032 3914
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 4304 5849 1132
Size: 97 3075 1545 2229
Fragments arranged by size:
3075 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 5845 5845
Size: 6946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6946
PfoI T'CCnGG_A
Cuts at: 2217 2217
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 2289 4516 2289
Size: 2227 4719
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3519 3551 3519
Size: 32 6914
PstI C_TGCA'G
Cuts at: 830 2284 2458 2682 2953 3316 3727 4298 830
Size: 1454 174 224 271 363 411 571 3478
Fragments arranged by size:
3478 1454 571 411 363 271 224 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 3214 3434 4108 3214
Size: 220 674 6052
Fragments arranged by size:
6052 674 220
SacI G_AGCT'C
Cuts at: 4011 4011
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 3944 5194 6906 3944
Size: 1250 1712 3984
Fragments arranged by size:
3984 1712 1250
SfcI C'TryA_G
Cuts at: 826 1045 2280 2454 2678 2949 3312 3723 3959
Size: 219 1235 174 224 271 363 411 236
Cuts at: 3959 4294 5429 6110 6301 826
Size: 335 1135 681 191 1471
Fragments arranged by size:
1471 1235 1135 681 411 363 335 271
236 224 219 191 174
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5050 5951 6213 820
Size: 197 926 3107 901 262 1553
Fragments arranged by size:
3107 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2748 3270 3393 4385 513
Size: 552 1683 522 123 992 3074
Fragments arranged by size:
3074 1683 992 552 522 123
SwaI ATTT'AAAT
Cuts at: 4036 4036
Size: 6946
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2431 3108 3812 4973 4990 5143 5328
Size: 1255 851 677 704 1161 17 153 185
Cuts at: 5328 5773 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1161 851 704 677 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3702 3846 3942
Size: 274 80 33 51 1443 1725 144 96
Cuts at: 3942 5192 6904 96
Size: 1250 1712 138
Fragments arranged by size:
1725 1712 1443 1250 274 144 138 96
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3723 3755 6906 6938 3723
Size: 32 3151 32 3731
Fragments arranged by size:
3731 3151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 3111 5075 3111
Size: 1964 4982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI XcmI