Restriction map for
pFN21AB5762
(Circular) (Six-base) MAPSORT of: pFN21AB5762.seq Check: 5869 from: 1 to: 6958
pFN21AB5762 6958 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2698 3103 2698
Size: 405 6553
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4768 278
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3378 3558 4301 1937
Size: 1441 180 743 4594
Fragments arranged by size:
4594 1441 743 180
Acc65I G'GTAC_C
Cuts at: 2403 3861 4279 2403
Size: 1458 418 5082
Fragments arranged by size:
5082 1458 418
AclI AA'CG_TT
Cuts at: 1563 5085 5458 1563
Size: 3522 373 3063
Fragments arranged by size:
3522 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2917 3359 5021 6405 6673 1632
Size: 515 770 442 1662 1384 268 1917
Fragments arranged by size:
1917 1662 1384 770 515 442 268
AfeI AGC'GCT
Cuts at: 2618 4187 2618
Size: 1569 5389
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 5857 6834 1203
Size: 4654 977 1327
Fragments arranged by size:
4654 1327 977
AgeI A'CCGG_T
Cuts at: 5789 5789
Size: 6958
AhdI GACnn_n'nnGTC
Cuts at: 5687 5687
Size: 6958
AleI CACnn'nnGTG
Cuts at: 1166 2561 3275 1166
Size: 1395 714 4849
Fragments arranged by size:
4849 1395 714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2969 3521 1184
Size: 1785 552 4621
Fragments arranged by size:
4621 1785 552
AlwNI CAG_nnn'CTG
Cuts at: 2687 3013 3352 3598 3817 4209 4701 6273 2687
Size: 326 339 246 219 392 492 1572 3372
Fragments arranged by size:
3372 1572 492 392 339 326 246 219
ApaI G_GGCC'C
Cuts at: 2358 2370 2358
Size: 12 6946
ApaLI G'TGCA_C
Cuts at: 2135 3020 3545 5015 6171 2135
Size: 885 525 1470 1156 2922
Fragments arranged by size:
2922 1470 1156 885 525
ApoI r'AATT_y
Cuts at: 1493 4267 4493 4645 6754 1493
Size: 2774 226 152 2109 1697
Fragments arranged by size:
2774 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5512 160
Size: 872 4480 1606
Fragments arranged by size:
4480 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2571 3243 3479 3637 4158 4283
Size: 73 128 500 672 236 158 521 125
Cuts at: 4283 1870
Size: 4545
Fragments arranged by size:
4545 672 521 500 236 158 128 125
73
BamHI G'GATC_C
Cuts at: 4191 4288 4655 4191
Size: 97 367 6494
Fragments arranged by size:
6494 367 97
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2403 3277 3575 3861 4279
Size: 325 217 627 616 874 298 286 418
Cuts at: 4279 5734 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 874 627 616 418 325 298
286 217
BanII G_rGCy'C
Cuts at: 1391 2067 2358 2370 2661 2741 3250 3638 4277
Size: 676 291 12 291 80 509 388 639
Cuts at: 4277 1391
Size: 4072
Fragments arranged by size:
4072 676 639 509 388 291 291 80
12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5987
BbvCI CC'TCA_GC
Cuts at: 2165 2252 2165
Size: 87 6871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3234 3268 5149 5183 1903
Size: 34 50 34 1213 34 1881 34 3678
Fragments arranged by size:
3678 1881 1213 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4850 6060 4850
Size: 1210 5748
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6958
BfrBI ATG'CAT
Cuts at: 3442 3442
Size: 6958
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3286 5569
Size: 107 122 71 631 605 337 1277 2283
Cuts at: 5569 136
Size: 1525
Fragments arranged by size:
2283 1525 1277 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6953 6953
Size: 6958
BlpI GC'TnA_GC
Cuts at: 4375 4375
Size: 6958
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2358 2370 2694 3024 3282 3549 5019
Size: 349 219 12 324 330 258 267 1470
Cuts at: 5019 6175 1790
Size: 1156 2573
Fragments arranged by size:
2573 1470 1156 349 330 324 267 258
219 12
BmgBI CAC'GTC
Cuts at: 1318 2782 1318
Size: 1464 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2658 2704 4689
Size: 416 53 83 399 24 1241 46 1985
Cuts at: 4689 5647 442
Size: 958 1753
Fragments arranged by size:
1985 1753 1241 958 416 399 83 53
46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6958
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4138 4138
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2164 2675 2813 2960 2989 3652 3686 5618
Size: 925 511 138 147 29 663 34 1932
Cuts at: 5618 1239
Size: 2579
Fragments arranged by size:
2579 1932 925 663 511 147 138 34
29
Bpu10I CC'TnA_GC
Cuts at: 2111 2165 2252 2111
Size: 54 87 6817
Fragments arranged by size:
6817 87 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5083 5948 6246 5083
Size: 865 298 5795
Fragments arranged by size:
5795 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3176 5621 882
Size: 617 1677 2445 2219
Fragments arranged by size:
2445 2219 1677 617
BsaAI yAC'GTr
Cuts at: 493 1204 4749 493
Size: 711 3545 2702
Fragments arranged by size:
3545 2702 711
BsaBI GATnn'nnATC
Cuts at: 6952 6952
Size: 6958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4765 5147 5782 275
Size: 53 83 186 936 3232 382 635 1451
Fragments arranged by size:
3232 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5391 5789 6063 6210 6621 5391
Size: 398 274 147 411 5728
Fragments arranged by size:
5728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 2696 2726 1178
Size: 30 165 30 993 30 270 30 5410
Fragments arranged by size:
5410 993 270 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2651 2842 2849 2983 2986 3851 1186
Size: 1258 207 191 7 134 3 865 4293
Fragments arranged by size:
4293 1258 865 207 191 134 7 3
BseYI C'CCAG_C
Cuts at: 2019 2687 2879 3129 6161 6657 2019
Size: 668 192 250 3032 496 2320
Fragments arranged by size:
3032 2320 668 496 250 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 3491 3922 4010 1163
Size: 573 888 867 431 88 4111
Fragments arranged by size:
4111 888 867 573 431 88
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 2386 2661 2681 2958 3024 3549 5019 5104
Size: 247 275 20 277 66 525 1470 85
Cuts at: 5104 6175 2139
Size: 1071 2922
Fragments arranged by size:
2922 1470 1071 525 277 275 247 85
66 20
BsmI GAATG_Cn'
Cuts at: 4469 4562 4469
Size: 93 6865
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2139 2358 2370 2386 2661 2681
Size: 399 277 72 219 12 16 275 20
Cuts at: 2681 2694 2741 2958 3024 3250 3282 3549 3638
Size: 13 47 217 66 226 32 267 89
Cuts at: 3638 4277 5019 5104 6175 1391
Size: 639 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 277 275 267
226 219 217 89 85 72 66 47
32 20 16 13 12
BspEI T'CCGG_A
Cuts at: 6621 6621
Size: 6958
BspHI T'CATG_A
Cuts at: 3151 4847 6577 3151
Size: 1696 1730 3532
Fragments arranged by size:
3532 1730 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2698 2998 3103 3741 4313 844
Size: 1854 300 105 638 572 3489
Fragments arranged by size:
3489 1854 638 572 300 105
BsrBI CCG'CTC
Cuts at: 1394 1823 2898 4845 1394
Size: 429 1075 1947 3507
Fragments arranged by size:
3507 1947 1075 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3039 5453 5627 65
Size: 1183 1791 2414 174 1396
Fragments arranged by size:
2414 1791 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 5602 5789 1700
Size: 110 198 3594 187 2869
Fragments arranged by size:
3594 2869 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6958
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 2663 5018 6030 2663
Size: 2355 1012 3591
Fragments arranged by size:
3591 2355 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2284 2447 3174 794
Size: 1490 163 727 4578
Fragments arranged by size:
4578 1490 727 163
BstBI TT'CG_AA
Cuts at: 5762 5762
Size: 6958
BstEII G'GTnAC_C
Cuts at: 1184 2315 1184
Size: 1131 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2653 3196 3521 2194
Size: 459 543 325 5631
Fragments arranged by size:
5631 543 459 325
BstYI r'GATC_y
Cuts at: 1972 3792 4191 4288 4655 5040 5057 6509 6595
Size: 1820 399 97 367 385 17 1452 86
Cuts at: 6595 6607 6953 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1820 1452 399 385 367 346 97
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4197 4197
Size: 6958
BtgI C'CryG_G
Cuts at: 513 1065 2025 2055 2193 2700 2866 2920 3493
Size: 552 960 30 138 507 166 54 573
Cuts at: 3493 3801 3945 4077 513
Size: 308 144 132 3394
Fragments arranged by size:
3394 960 573 552 507 308 166 144
138 132 54 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6219
Fragments arranged by size:
6219 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 3075 3596 3879 4474 5268 5288 2139
Size: 936 521 283 595 794 20 3809
Fragments arranged by size:
3809 936 794 595 521 283 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6923
DraI TTT'AAA
Cuts at: 3310 4262 3310
Size: 952 6006
DraIII CAC_nnn'GTG
Cuts at: 3544 3649 3544
Size: 105 6853
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2786 5965 809
Size: 507 1470 3179 1802
Fragments arranged by size:
3179 1802 1470 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2196 2587
Size: 54 1425 50 275 18 222 144 391
Cuts at: 2587 3804 4094 4405 5294 6923 8
Size: 1217 290 311 889 1629 43
Fragments arranged by size:
1629 1425 1217 889 391 311 290 275
222 144 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2852 4888 1353
Size: 1499 2036 3423
Fragments arranged by size:
3423 2036 1499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2075 3045 5545 5919 6065 387
Size: 1064 138 486 970 2500 374 146 1280
Fragments arranged by size:
2500 1280 1064 970 486 374 146 138
EcoICRI GAG'CTC
Cuts at: 2659 2659
Size: 6958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2164 2675 2813 2917 2960 2989
Size: 393 515 17 511 138 104 43 29
Cuts at: 2989 3359 3652 3686 5021 5618 6405 6673 1239
Size: 370 293 34 1335 597 787 268 1524
Fragments arranged by size:
1524 1335 787 597 515 511 393 370
293 268 138 104 43 34 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 3856 3856
Size: 6958
EcoO109I rG'GnC_Cy
Cuts at: 2354 2355 2366 2367 2797 3010 3133 3937 3994
Size: 1 11 1 430 213 123 804 57
Cuts at: 3994 4117 4242 2354
Size: 123 125 5070
Fragments arranged by size:
5070 804 430 213 125 123 123 57
11 1 1
EcoRI G'AATT_C
Cuts at: 4267 4645 4267
Size: 378 6580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 6958
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 2596 2620 3077 3694 4189 6105 1678
Size: 918 24 457 617 495 1916 2531
Fragments arranged by size:
2531 1916 918 617 495 457 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5599 5631 5673 5705 1373
Size: 32 135 32 4027 32 42 32 2626
Fragments arranged by size:
4027 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4302 4548 669
Size: 988 281 2364 246 3079
Fragments arranged by size:
3079 2364 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6958
HpaI GTT'AAC
Cuts at: 4548 4548
Size: 6958
KpnI G_GTAC'C
Cuts at: 2407 3865 4283 2407
Size: 1458 418 5082
Fragments arranged by size:
5082 1458 418
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3327 6072 6256 1305
Size: 2022 2745 184 2007
Fragments arranged by size:
2745 2022 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 2589 10
Size: 54 1425 343 366 391 4379
Fragments arranged by size:
4379 1425 391 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2192 2222 2331 2561 2891 3213
Size: 648 873 153 30 109 230 330 322
Cuts at: 3213 3275 4916 5275 5434 518
Size: 62 1641 359 159 2042
Fragments arranged by size:
2042 1641 873 648 359 330 322 230
159 153 109 62 30
MspA1I CmG'CkG
Cuts at: 1606 2803 2868 4374 5051 6199 6444 1606
Size: 1197 65 1506 677 1148 245 2120
Fragments arranged by size:
2120 1506 1197 1148 677 245 65
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 6958
NcoI C'CATG_G
Cuts at: 513 1065 2055 2193 3801 513
Size: 552 990 138 1608 3670
Fragments arranged by size:
3670 1608 990 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6958
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 6958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6958
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NsiI A_TGCA'T
Cuts at: 3444 3444
Size: 6958
NspI r_CATG'y
Cuts at: 1132 1229 2380 3106 3446 4316 5861 1132
Size: 97 1151 726 340 870 1545 2229
Fragments arranged by size:
2229 1545 1151 870 726 340 97
PasI CC'CwG_GG
Cuts at: 1348 2014 3762 3933 1348
Size: 666 1748 171 4373
Fragments arranged by size:
4373 1748 666 171
PciI A'CATG_T
Cuts at: 5857 5857
Size: 6958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3352 3630 3802 3852 1108
Size: 2244 278 172 50 4214
Fragments arranged by size:
4214 2244 278 172 50
PfoI T'CCnGG_A
Cuts at: 2790 3195 2790
Size: 405 6553
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 6540 6572 5027
Size: 32 1481 32 5413
Fragments arranged by size:
5413 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 3010 3937 3994 4117 4242 2797
Size: 213 927 57 123 125 5513
Fragments arranged by size:
5513 927 213 125 123 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6958
PsiI TTA'TAA
Cuts at: 4528 4528
Size: 6958
PspOMI G'GGCC_C
Cuts at: 2354 2366 2354
Size: 12 6946
PstI C_TGCA'G
Cuts at: 830 2049 2175 2472 3009 3100 3603 3792 4310
Size: 1219 126 297 537 91 503 189 518
Cuts at: 4310 830
Size: 3478
Fragments arranged by size:
3478 1219 537 518 503 297 189 126
91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
SacI G_AGCT'C
Cuts at: 2661 2661
Size: 6958
SacII CC_GC'GG
Cuts at: 2869 2869
Size: 6958
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SanDI GG'GwC_CC
Cuts at: 3937 3937
Size: 6958
SbfI CC_TGCA'GG
Cuts at: 2472 3100 4310 2472
Size: 628 1210 5120
Fragments arranged by size:
5120 1210 628
ScaI AGT'ACT
Cuts at: 3680 5206 6918 3680
Size: 1526 1712 3720
Fragments arranged by size:
3720 1712 1526
SexAI A'CCwGG_T
Cuts at: 3810 3996 3810
Size: 186 6772
SfcI C'TryA_G
Cuts at: 826 1045 2045 2171 2468 3005 3096 3599 3788
Size: 219 1000 126 297 537 91 503 189
Cuts at: 3788 4246 4306 5441 6122 6313 826
Size: 458 60 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 537 503 458 297
219 191 189 126 91 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 2073 2573 4285 1872
Size: 201 500 1712 4545
Fragments arranged by size:
4545 1712 500 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5062 5963 6225 820
Size: 197 926 3119 901 262 1553
Fragments arranged by size:
3119 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6958
SphI G_CATG'C
Cuts at: 1132 3106 3446 4316 1132
Size: 1974 340 870 3774
Fragments arranged by size:
3774 1974 870 340
SspI AAT'ATT
Cuts at: 5 52 4882 5
Size: 47 4830 2081
Fragments arranged by size:
4830 2081 47
StuI AGG'CCT
Cuts at: 1517 2398 1517
Size: 881 6077
StyI C'CwwG_G
Cuts at: 513 1065 2055 2193 3801 4397 513
Size: 552 990 138 1608 596 3074
Fragments arranged by size:
3074 1608 990 596 552 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3997 4985 5002 5155 5340 5785
Size: 1255 1947 470 988 17 153 185 445
Cuts at: 5785 325
Size: 1498
Fragments arranged by size:
1947 1498 1255 988 470 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3678 5204 6916
Size: 274 80 33 51 1443 1701 1526 1712
Cuts at: 6916 96
Size: 138
Fragments arranged by size:
1712 1701 1526 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 3284 3316 6918 6950 2696
Size: 32 556 32 3602 32 2704
Fragments arranged by size:
3602 2704 556 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3230 1316
Size: 1914 5044
XbaI T'CTAG_A
Cuts at: 4294 4294
Size: 6958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3424 2011
Size: 1413 5545
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 2071 2571 4283 1870
Size: 201 500 1712 4545
Fragments arranged by size:
4545 1712 500 201
XmnI GAAnn'nnTTC
Cuts at: 3587 5087 3587
Size: 1500 5458
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4766 276
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI
SwaI