Restriction map for
pFN21AB5758
(Circular) (Six-base) MAPSORT of: pFN21AB5758.seq Check: 3418 from: 1 to: 6922
pFN21AB5758 6922 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 2277 2361 2589 3761 3945 4265 1937
Size: 334 6 84 228 1172 184 320 4594
Fragments arranged by size:
4594 1172 334 320 228 184 84 6
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3657 3802 4985 6369 6637 1632
Size: 776 1249 145 1183 1384 268 1917
Fragments arranged by size:
1917 1384 1249 1183 776 268 145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 5821 6798 1203
Size: 4618 977 1327
Fragments arranged by size:
4618 1327 977
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 1184
Size: 1597 5325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3765 3797 3765
Size: 32 6890
AlwNI CAG_nnn'CTG
Cuts at: 2291 2530 2903 4665 6237 2291
Size: 239 373 1762 1572 2976
Fragments arranged by size:
2976 1762 1572 373 239
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 2594 2923 2986 3038 3620 4231 4457 4609
Size: 1101 329 63 52 582 611 226 152
Cuts at: 4609 6718 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1101 611 582 329 226 152
63 52
AseI AT'TA_AT
Cuts at: 160 1032 3207 5476 160
Size: 872 2175 2269 1606
Fragments arranged by size:
2269 2175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 1870
Size: 73 2304 4545
Fragments arranged by size:
4545 2304 73
BamHI G'GATC_C
Cuts at: 4252 4619 4252
Size: 367 6555
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 4243 5698 618
Size: 325 217 627 410 2046 1455 1842
Fragments arranged by size:
2046 1842 1455 627 410 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 4241 1391
Size: 907 1943 4072
Fragments arranged by size:
4072 1943 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5951
BbvCI CC'TCA_GC
Cuts at: 2111 2184 2111
Size: 73 6849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2122 2156 5113 5147 1903
Size: 34 50 34 101 34 2957 34 3678
Fragments arranged by size:
3678 2957 101 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4182 4814 6024 4182
Size: 632 1210 5080
Fragments arranged by size:
5080 1210 632
BclI T'GATC_A
Cuts at: 1548 2407 3024 3669 1548
Size: 859 617 645 4801
Fragments arranged by size:
4801 859 645 617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2071 5533 136
Size: 107 122 71 631 605 399 3462 1525
Fragments arranged by size:
3462 1525 631 605 399 122 107 71
BglII A'GATC_T
Cuts at: 2230 3003 4098 6917 2230
Size: 773 1095 2819 2235
Fragments arranged by size:
2819 2235 1095 773
BlpI GC'TnA_GC
Cuts at: 2292 4339 2292
Size: 2047 4875
Bme1580I G_kGCm'C
Cuts at: 1790 4983 6139 1790
Size: 3193 1156 2573
Fragments arranged by size:
3193 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 3495 4653
Size: 416 53 83 399 24 1652 426 1158
Cuts at: 4653 5611 442
Size: 958 1753
Fragments arranged by size:
1753 1652 1158 958 426 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2213 1056
Size: 1157 5765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2518 2552 2811 2889 3258 5582 1239
Size: 1279 34 259 78 369 2324 2579
Fragments arranged by size:
2579 2324 1279 369 259 78 34
Bpu10I CC'TnA_GC
Cuts at: 2111 2184 2559 2111
Size: 73 375 6474
Fragments arranged by size:
6474 375 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2911 5047 5912 6210 2911
Size: 2136 865 298 3623
Fragments arranged by size:
3623 2136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2887 5585 882
Size: 617 1388 2698 2219
Fragments arranged by size:
2698 2219 1388 617
BsaAI yAC'GTr
Cuts at: 493 1204 4713 493
Size: 711 3509 2702
Fragments arranged by size:
3509 2702 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4729 5111 5746 275
Size: 53 83 186 936 3196 382 635 1451
Fragments arranged by size:
3196 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5355 5753 6027 6174 6585 5355
Size: 398 274 147 411 5692
Fragments arranged by size:
5692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2076 2106 2220 2250 2321
Size: 30 165 30 673 30 114 30 71
Cuts at: 2321 2351 3174 3204 4154 4184 1178
Size: 30 823 30 950 30 3916
Fragments arranged by size:
3916 950 823 673 165 114 71 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2407 1186
Size: 1015 206 5701
Fragments arranged by size:
5701 1015 206
BseYI C'CCAG_C
Cuts at: 2736 4112 6125 6621 2736
Size: 1376 2013 496 3037
Fragments arranged by size:
3037 2013 1376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2889 1163
Size: 573 1153 5196
Fragments arranged by size:
5196 1153 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2041 2298 3063 4983 5068 6139 2041
Size: 257 765 1920 85 1071 2824
Fragments arranged by size:
2824 1920 1071 765 257 85
BsmI GAATG_Cn'
Cuts at: 4433 4526 4433
Size: 93 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2298 3063 4241 4983 5068 6139
Size: 399 251 257 765 1178 742 85 1071
Cuts at: 6139 1391
Size: 2174
Fragments arranged by size:
2174 1178 1071 765 742 399 257 251
85
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4277 844
Size: 3433 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2048 3164 4809 1394
Size: 429 225 1116 1645 3507
Fragments arranged by size:
3507 1645 1116 429 225
BsrDI GCAATG_nn'
Cuts at: 65 1248 5417 5591 65
Size: 1183 4169 174 1396
Fragments arranged by size:
4169 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 5566 5753 1700
Size: 110 293 3463 187 2869
Fragments arranged by size:
3463 2869 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6922
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 3423 4982 5994 3423
Size: 1559 1012 4351
Fragments arranged by size:
4351 1559 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6922
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstYI r'GATC_y
Cuts at: 1972 2230 2526 2770 3003 3070 4098 4252 4619
Size: 258 296 244 233 67 1028 154 367
Cuts at: 4619 5004 5021 6473 6559 6571 6917 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1028 385 367 346 296 258
244 233 154 86 67 17 12
BstZ17I GTA'TAC
Cuts at: 3762 3762
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6183
Fragments arranged by size:
6183 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 2292 4050 4438 5232 5252 2220
Size: 72 1758 388 794 20 3890
Fragments arranged by size:
3890 1758 794 388 72 20
ClaI AT'CG_AT
Cuts at: 1554 2538 1554
Size: 984 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 2571 4226 2571
Size: 1655 5267
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 3235 4369
Size: 54 1425 50 275 18 751 654 1134
Cuts at: 4369 5258 6887 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1134 889 751 654 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 3674 4852 1353
Size: 1031 1290 1178 3423
Fragments arranged by size:
3423 1290 1178 1031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 5509 5883 6029 387
Size: 1064 138 501 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 501 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 6922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2518 2552 2811 2889 3258 3657
Size: 393 776 110 34 259 78 369 399
Cuts at: 3657 3802 4985 5582 6369 6637 1239
Size: 145 1183 597 787 268 1524
Fragments arranged by size:
1524 1183 787 776 597 399 393 369
268 259 145 110 78 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3257 3257
Size: 6922
EcoRI G'AATT_C
Cuts at: 3038 3620 4231 4609 3038
Size: 582 611 378 5351
Fragments arranged by size:
5351 611 582 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3458 3458
Size: 6922
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 3976 6069 1678
Size: 2298 2093 2531
Fragments arranged by size:
2531 2298 2093
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2995 3027 3332
Size: 32 135 32 504 32 887 32 305
Cuts at: 3332 3364 3541 3573 3938 3970 4058 4083 4090
Size: 32 177 32 365 32 88 25 7
Cuts at: 4090 4115 5563 5595 5637 5669 1373
Size: 25 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 887 504 365 305 177 135
88 42 32 32 32 32 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 4266 4512 669
Size: 988 281 1719 609 246 3079
Fragments arranged by size:
3079 1719 988 609 281 246
HindIII A'AGCT_T
Cuts at: 748 2412 748
Size: 1664 5258
HpaI GTT'AAC
Cuts at: 3657 4512 3657
Size: 855 6067
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 3513 3627 4521 3513
Size: 114 894 5914
Fragments arranged by size:
5914 894 114
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3244 3347 3361 6036 6220 1305
Size: 1939 103 14 2675 184 2007
Fragments arranged by size:
2675 2007 1939 184 103 14
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2583 10
Size: 54 1425 343 751 4349
Fragments arranged by size:
4349 1425 751 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3847 4880 5239 5398 518
Size: 648 2681 1033 359 159 2042
Fragments arranged by size:
2681 2042 1033 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3153 4338 5015 6163 6408 1606
Size: 1547 1185 677 1148 245 2120
Fragments arranged by size:
2120 1547 1185 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 2209 1052
Size: 1157 5765
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2881 4280 5825 1132
Size: 97 1652 1399 1545 2229
Fragments arranged by size:
2229 1652 1545 1399 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 5821 5821
Size: 6922
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3106 1108
Size: 1998 4924
PfoI T'CCnGG_A
Cuts at: 2156 2529 2156
Size: 373 6549
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PstI C_TGCA'G
Cuts at: 830 4274 830
Size: 3444 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 6922
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SfcI C'TryA_G
Cuts at: 826 1045 3831 4270 5405 6086 6277 826
Size: 219 2786 439 1135 681 191 1471
Fragments arranged by size:
2786 1471 1135 681 439 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2890 5026 5927 6189 820
Size: 197 926 947 2136 901 262 1553
Fragments arranged by size:
2136 1553 947 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 4280 1132
Size: 3148 3774
SspI AAT'ATT
Cuts at: 5 52 2485 4846 5
Size: 47 2433 2361 2081
Fragments arranged by size:
2433 2361 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6922
StyI C'CwwG_G
Cuts at: 513 1065 2950 4361 513
Size: 552 1885 1411 3074
Fragments arranged by size:
3074 1885 1411 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2379 4949 4966 5119 5304 5749 325
Size: 1255 799 2570 17 153 185 445 1498
Fragments arranged by size:
2570 1498 1255 799 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5168 6880 96
Size: 274 80 33 51 1443 3191 1712 138
Fragments arranged by size:
3191 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 6882 6914 2220
Size: 32 4630 32 2228
Fragments arranged by size:
4630 2228 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6922
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 2637 3683 3714 5051 2637
Size: 1046 31 1337 4508
Fragments arranged by size:
4508 1337 1046 31
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI