Restriction map for
pFN21AB5753
(Circular) (Six-base) MAPSORT of: pFN21AB5753.seq Check: 2162 from: 1 to: 6973
pFN21AB5753 6973 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 1937
Size: 2379 4594
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 2448 2966 2984 3695 3875 4016 5036
Size: 801 15 518 18 711 180 141 1020
Cuts at: 5036 6420 6688 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1020 801 711 518 268 180
141 18 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 3285 5872 6849 1203
Size: 2082 2587 977 1327
Fragments arranged by size:
2587 2082 1327 977
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6973
AlwNI CAG_nnn'CTG
Cuts at: 4716 6288 4716
Size: 1572 5401
ApaLI G'TGCA_C
Cuts at: 2843 3053 5030 6186 2843
Size: 210 1977 1156 3630
Fragments arranged by size:
3630 1977 1156 210
ApoI r'AATT_y
Cuts at: 1493 2707 2779 3508 4116 4172 4262 4282 4508
Size: 1214 72 729 608 56 90 20 226
Cuts at: 4508 4660 6769 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1214 729 608 226 152 90
72 56 20
AseI AT'TA_AT
Cuts at: 160 1032 2943 3738 5527 160
Size: 872 1911 795 1789 1606
Fragments arranged by size:
1911 1789 1606 872 795
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 4298 1870
Size: 73 2355 4545
Fragments arranged by size:
4545 2355 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2688 2721 2688
Size: 33 6940
BamHI G'GATC_C
Cuts at: 4303 4670 4303
Size: 367 6606
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4294 5749 618
Size: 325 217 627 2507 1455 1842
Fragments arranged by size:
2507 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3149 4292 1391
Size: 1758 1143 4072
Fragments arranged by size:
4072 1758 1143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3240 3534 3648 928
Size: 971 1341 294 114 4253
Fragments arranged by size:
4253 1341 971 294 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5164 5198 1903
Size: 34 3227 34 3678
Fragments arranged by size:
3678 3227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3379 4865 6075 3379
Size: 1486 1210 4277
Fragments arranged by size:
4277 1486 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6973
BfrBI ATG'CAT
Cuts at: 3464 3464
Size: 6973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 5584 136
Size: 107 122 71 631 605 382 3530 1525
Fragments arranged by size:
3530 1525 631 605 382 122 107 71
BglII A'GATC_T
Cuts at: 2018 2337 2753 6968 2018
Size: 319 416 4215 2023
Fragments arranged by size:
4215 2023 416 319
BlpI GC'TnA_GC
Cuts at: 4390 4390
Size: 6973
Bme1580I G_kGCm'C
Cuts at: 1790 2847 3057 5034 6190 1790
Size: 1057 210 1977 1156 2573
Fragments arranged by size:
2573 1977 1156 1057 210
BmgBI CAC'GTC
Cuts at: 1318 3288 1318
Size: 1970 5003
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 2351 4704
Size: 416 53 83 399 24 752 182 2353
Cuts at: 4704 5662 442
Size: 958 1753
Fragments arranged by size:
2353 1753 958 752 416 399 182 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3939 5633 1239
Size: 2700 1694 2579
Fragments arranged by size:
2700 2579 1694
Bpu10I CC'TnA_GC
Cuts at: 2031 2031
Size: 6973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5098 5963 6261 5098
Size: 865 298 5810
Fragments arranged by size:
5810 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 5636 882
Size: 617 756 3381 2219
Fragments arranged by size:
3381 2219 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 4764 493
Size: 711 3560 2702
Fragments arranged by size:
3560 2702 711
BsaBI GATnn'nnATC
Cuts at: 6967 6967
Size: 6973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4780 5162 5797 275
Size: 53 83 186 936 3247 382 635 1451
Fragments arranged by size:
3247 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5406 5804 6078 6225 6636 5406
Size: 398 274 147 411 5743
Fragments arranged by size:
5743 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6748
Fragments arranged by size:
6748 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6973
BseYI C'CCAG_C
Cuts at: 6176 6672 6176
Size: 496 6477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2007 1163
Size: 573 271 6129
Fragments arranged by size:
6129 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 2847 3057 5034 5119 6190 2301
Size: 546 210 1977 85 1071 3084
Fragments arranged by size:
3084 1977 1071 546 210 85
BsmI GAATG_Cn'
Cuts at: 4484 4577 4484
Size: 93 6880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2847 3057 3149 4292 5034 5119
Size: 399 511 546 210 92 1143 742 85
Cuts at: 5119 6190 1391
Size: 1071 2174
Fragments arranged by size:
2174 1143 1071 742 546 511 399 210
92 85
BspEI T'CCGG_A
Cuts at: 6636 6636
Size: 6973
BspHI T'CATG_A
Cuts at: 4862 6592 4862
Size: 1730 5243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4328 844
Size: 3484 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4860 1394
Size: 429 3037 3507
Fragments arranged by size:
3507 3037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4276 5468 5642 65
Size: 1183 3028 1192 174 1396
Fragments arranged by size:
3028 1396 1192 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5617 5804 1700
Size: 110 3807 187 2869
Fragments arranged by size:
3807 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 5033 6045 5033
Size: 1012 5961
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4229 794
Size: 3435 3538
BstBI TT'CG_AA
Cuts at: 5777 5777
Size: 6973
BstEII G'GTnAC_C
Cuts at: 1184 2011 1184
Size: 827 6146
BstYI r'GATC_y
Cuts at: 1972 2018 2123 2337 2521 2753 4303 4670 5055
Size: 46 105 214 184 232 1550 367 385
Cuts at: 5055 5072 6524 6610 6622 6968 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1550 1452 385 367 346 232 214
184 105 86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5482
Fragments arranged by size:
5482 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6234
Fragments arranged by size:
6234 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4489 5283 5303 3349
Size: 1140 794 20 5019
Fragments arranged by size:
5019 1140 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6938
DraI TTT'AAA
Cuts at: 4277 4277
Size: 6973
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3412 4420 5309
Size: 54 1425 50 275 18 1582 1008 889
Cuts at: 5309 6938 8
Size: 1629 43
Fragments arranged by size:
1629 1582 1425 1008 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 3201 3375 3516 3561 3567 4903 1353
Size: 848 1000 174 141 45 6 1336 3423
Fragments arranged by size:
3423 1336 1000 848 174 141 45 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5560 5934 6080 387
Size: 1064 138 3971 374 146 1280
Fragments arranged by size:
3971 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2433 2448 2966 2984 3695 3875 3939
Size: 393 801 15 518 18 711 180 64
Cuts at: 3939 4016 5036 5633 6420 6688 1239
Size: 77 1020 597 787 268 1524
Fragments arranged by size:
1524 1020 801 787 711 597 518 393
268 180 77 64 18 15
EcoRI G'AATT_C
Cuts at: 2707 4116 4172 4282 4660 2707
Size: 1409 56 110 378 5020
Fragments arranged by size:
5020 1409 378 110 56
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 6120 1678
Size: 4442 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2795 2827 5614
Size: 32 135 32 689 32 502 32 2787
Cuts at: 5614 5646 5688 5720 1373
Size: 32 42 32 2626
Fragments arranged by size:
2787 2626 689 502 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3681 4087 4317 4563 669
Size: 988 281 1743 406 230 246 3079
Fragments arranged by size:
3079 1743 988 406 281 246 230
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6973
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 4572 4572
Size: 6973
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2417 6087 6271 1305
Size: 1112 3670 184 2007
Fragments arranged by size:
3670 2007 1112 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3414 10
Size: 54 1425 343 1582 3569
Fragments arranged by size:
3569 1582 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4931 5290 5449 518
Size: 648 3765 359 159 2042
Fragments arranged by size:
3765 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2962 4097 4389 5066 6214 6459 1606
Size: 1356 1135 292 677 1148 245 2120
Fragments arranged by size:
2120 1356 1148 1135 677 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5482
Fragments arranged by size:
5482 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NsiI A_TGCA'T
Cuts at: 3466 3466
Size: 6973
NspI r_CATG'y
Cuts at: 1132 1229 2352 4331 5876 1132
Size: 97 1123 1979 1545 2229
Fragments arranged by size:
2229 1979 1545 1123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 5872 5872
Size: 6973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6973
PfoI T'CCnGG_A
Cuts at: 3487 3487
Size: 6973
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3702 1613
Size: 2089 4884
PsiI TTA'TAA
Cuts at: 3221 4543 3221
Size: 1322 5651
PstI C_TGCA'G
Cuts at: 830 3819 4325 830
Size: 2989 506 3478
Fragments arranged by size:
3478 2989 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 2962 4097 2962
Size: 1135 5838
SalI G'TCGA_C
Cuts at: 1936 4315 1936
Size: 2379 4594
SapI GCTCTTCn'nnn_
Cuts at: 2201 2201
Size: 6973
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 2119 4064 4199 5221 6933 2119
Size: 1945 135 1022 1712 2159
Fragments arranged by size:
2159 1945 1712 1022 135
SfcI C'TryA_G
Cuts at: 826 1045 2217 2484 3758 3815 4321 5456 6137
Size: 219 1172 267 1274 57 506 1135 681
Cuts at: 6137 6328 826
Size: 191 1471
Fragments arranged by size:
1471 1274 1172 1135 681 506 267 219
191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6973
SmaI CCC'GGG
Cuts at: 1872 4300 1872
Size: 2428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5077 5978 6240 820
Size: 197 926 3134 901 262 1553
Fragments arranged by size:
3134 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4764 493
Size: 4271 2702
SpeI A'CTAG_T
Cuts at: 152 3331 152
Size: 3179 3794
SphI G_CATG'C
Cuts at: 1132 2352 4331 1132
Size: 1220 1979 3774
Fragments arranged by size:
3774 1979 1220
SspI AAT'ATT
Cuts at: 5 52 4897 5
Size: 47 4845 2081
Fragments arranged by size:
4845 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6973
StyI C'CwwG_G
Cuts at: 513 1065 2004 4412 513
Size: 552 939 2408 3074
Fragments arranged by size:
3074 2408 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 5000 5017 5170 5355 5800 325
Size: 1255 2133 1287 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1287 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2117 4062 4197
Size: 274 80 33 51 1443 140 1945 135
Cuts at: 4197 5219 6931 96
Size: 1022 1712 138
Fragments arranged by size:
1945 1712 1443 1022 274 140 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6933 6965 6933
Size: 32 6941
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6973
XbaI T'CTAG_A
Cuts at: 2456 4309 2456
Size: 1853 5120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6973
XmaI C'CCGG_G
Cuts at: 1870 4298 1870
Size: 2428 4545
XmnI GAAnn'nnTTC
Cuts at: 5102 5102
Size: 6973
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI