Restriction map for
pFN21AB5752
(Circular) (Six-base) MAPSORT of: pFN21AB5752.seq Check: 9584 from: 1 to: 6946
pFN21AB5752 6946 bp
With 182 enzymes: SgfI *
June 12, 2017 10:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3999 4756 278
Size: 53 83 186 936 2463 757 2468
Fragments arranged by size:
2468 2463 936 757 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 4289 1937
Size: 922 1430 4594
Fragments arranged by size:
4594 1430 922
Acc65I G'GTAC_C
Cuts at: 2427 4267 2427
Size: 1840 5106
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2015 2806 3636 3831 4008 5009 6393 6661
Size: 383 791 830 195 177 1001 1384 268
Cuts at: 6661 1632
Size: 1917
Fragments arranged by size:
1917 1384 1001 830 791 383 268 195
177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 5845 6822 1203
Size: 4642 977 1327
Fragments arranged by size:
4642 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 2824 5675 2824
Size: 2851 4095
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6946
AlwNI CAG_nnn'CTG
Cuts at: 2202 3854 3931 3986 4689 6261 2202
Size: 1652 77 55 703 1572 2887
Fragments arranged by size:
2887 1652 1572 703 77 55
ApaI G_GGCC'C
Cuts at: 2333 2333
Size: 6946
ApaLI G'TGCA_C
Cuts at: 5003 6159 5003
Size: 1156 5790
ApoI r'AATT_y
Cuts at: 1493 2987 3253 4094 4255 4481 4633 6742 1493
Size: 1494 266 841 161 226 152 2109 1697
Fragments arranged by size:
2109 1697 1494 841 266 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 2679 2724 3392 3396 3462 4185 5500
Size: 872 1647 45 668 4 66 723 1315
Cuts at: 5500 160
Size: 1606
Fragments arranged by size:
1647 1606 1315 872 723 668 66 45
4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 2135 2146 2206 2337 4271 1870
Size: 73 192 11 60 131 1934 4545
Fragments arranged by size:
4545 1934 192 131 73 60 11
AvrII C'CTAG_G
Cuts at: 3191 3191
Size: 6946
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 2427 3195 4267 5722
Size: 325 217 627 395 245 768 1072 1455
Cuts at: 5722 618
Size: 1842
Fragments arranged by size:
1842 1455 1072 768 627 395 325 245
217
BanII G_rGCy'C
Cuts at: 1391 2147 2157 2333 4265 1391
Size: 756 10 176 1932 4072
Fragments arranged by size:
4072 1932 756 176 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 3318 3618 928
Size: 971 480 939 300 4256
Fragments arranged by size:
4256 971 939 480 300
BbvCI CC'TCA_GC
Cuts at: 3969 3969
Size: 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5137 5171 1903
Size: 34 3200 34 3678
Fragments arranged by size:
3678 3200 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 4171 1548
Size: 2623 4323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2328 5557 136
Size: 107 122 71 631 605 656 3229 1525
Fragments arranged by size:
3229 1525 656 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 6941 2869
Size: 4072 2874
BlpI GC'TnA_GC
Cuts at: 4363 4363
Size: 6946
Bme1580I G_kGCm'C
Cuts at: 1790 2333 3200 5007 6163 1790
Size: 543 867 1807 1156 2573
Fragments arranged by size:
2573 1807 1156 867 543
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3834 4677 5635
Size: 416 53 83 399 24 2417 843 958
Cuts at: 5635 442
Size: 1753
Fragments arranged by size:
2417 1753 958 843 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2805 4161 5606 1239
Size: 1566 1356 1445 2579
Fragments arranged by size:
2579 1566 1445 1356
Bpu10I CC'TnA_GC
Cuts at: 3969 3969
Size: 6946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2868 3601 5071 5936 6234 2868
Size: 733 1470 865 298 3580
Fragments arranged by size:
3580 1470 865 733 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 882
Size: 617 4110 2219
Fragments arranged by size:
4110 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4737 493
Size: 711 3533 2702
Fragments arranged by size:
3533 2702 711
BsaBI GATnn'nnATC
Cuts at: 3921 6940 3921
Size: 3019 3927
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3996 4753 5135 5770
Size: 53 83 186 936 2463 757 382 635
Cuts at: 5770 275
Size: 1451
Fragments arranged by size:
2463 1451 936 757 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3796 5379 5777 6051 6198 6609 3796
Size: 1583 398 274 147 411 4133
Fragments arranged by size:
4133 1583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 2110 2140 2620
Size: 30 165 30 660 30 17 30 480
Cuts at: 2620 2650 2778 2808 1178
Size: 30 128 30 5316
Fragments arranged by size:
5316 660 480 165 128 30 30 30
30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2411 2510 3171 3299 4158 1186
Size: 1222 3 99 661 128 859 3974
Fragments arranged by size:
3974 1222 859 661 128 99 3
BseYI C'CCAG_C
Cuts at: 2297 2789 3199 6149 6645 2297
Size: 492 410 2950 496 2598
Fragments arranged by size:
2950 2598 496 492 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5007 5092 6163 5007
Size: 85 1071 5790
Fragments arranged by size:
5790 1071 85
BsmI GAATG_Cn'
Cuts at: 4457 4550 4457
Size: 93 6853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2157 2333 3200 4265 5007 5092
Size: 399 357 10 176 867 1065 742 85
Cuts at: 5092 6163 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1065 867 742 399 357 176
85 10
BspEI T'CCGG_A
Cuts at: 3796 6609 3796
Size: 2813 4133
BspHI T'CATG_A
Cuts at: 3222 4835 6565 3222
Size: 1613 1730 3603
Fragments arranged by size:
3603 1730 1613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4301 844
Size: 3457 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 2140 4833 1394
Size: 429 278 39 2693 3507
Fragments arranged by size:
3507 2693 429 278 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 5441 5615 65
Size: 1183 4193 174 1396
Fragments arranged by size:
4193 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 5590 5777 1700
Size: 110 375 3405 187 2869
Fragments arranged by size:
3405 2869 375 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6946
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 2593 2869 2878 2917 4276 4643 5028 5045
Size: 621 276 9 39 1359 367 385 17
Cuts at: 5045 6497 6583 6595 6941 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1359 621 385 367 346 276
86 39 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 2114 2543 3961 4068 513
Size: 552 939 110 429 1418 107 3391
Fragments arranged by size:
3391 1418 939 552 429 110 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2903 487
Size: 683 56 1677 4530
Fragments arranged by size:
4530 1677 683 56
BtsI GCAGTG_nn'
Cuts at: 4462 5256 5276 4462
Size: 794 20 6132
Fragments arranged by size:
6132 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2628 2663 529
Size: 35 2064 35 4812
Fragments arranged by size:
4812 2064 35 35
DraI TTT'AAA
Cuts at: 4250 4250
Size: 6946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3452 5953 809
Size: 507 2136 2501 1802
Fragments arranged by size:
2501 2136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2352 4393
Size: 54 1425 50 275 18 363 159 2041
Cuts at: 4393 5282 6911 8
Size: 889 1629 43
Fragments arranged by size:
2041 1629 1425 889 363 275 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2397 3603 4876 1353
Size: 1044 1206 1273 3423
Fragments arranged by size:
3423 1273 1206 1044
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2255 5533 5907 6053 387
Size: 1064 138 435 231 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 435 374 231 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2805 2806 3636 3831 4008 4161
Size: 393 383 790 1 830 195 177 153
Cuts at: 4161 5009 5606 6393 6661 1239
Size: 848 597 787 268 1524
Fragments arranged by size:
1524 848 830 790 787 597 393 383
268 195 177 153 1
EcoO109I rG'GnC_Cy
Cuts at: 2329 2329
Size: 6946
EcoRI G'AATT_C
Cuts at: 4094 4255 4633 4094
Size: 161 378 6407
Fragments arranged by size:
6407 378 161
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 2228 3969 6093 1678
Size: 550 1741 2124 2531
Fragments arranged by size:
2531 2124 1741 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2110 2142 2492
Size: 32 135 32 491 32 15 32 350
Cuts at: 2492 2524 2810 2842 3778 3810 3986 4018 5587
Size: 32 286 32 936 32 176 32 1569
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 936 491 350 286 176 135
42 32 32 32 32 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3139 3304 4290 4536 669
Size: 988 281 1201 165 986 246 3079
Fragments arranged by size:
3079 1201 988 986 281 246 165
HindIII A'AGCT_T
Cuts at: 748 3521 748
Size: 2773 4173
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KpnI G_GTAC'C
Cuts at: 2431 4271 2431
Size: 1840 5106
MfeI C'AATT_G
Cuts at: 2730 4545 2730
Size: 1815 5131
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 6060 6244 1305
Size: 1323 3432 184 2007
Fragments arranged by size:
3432 2007 1323 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5124
Fragments arranged by size:
5124 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 3426 4904 5263 5422 518
Size: 648 1315 945 1478 359 159 2042
Fragments arranged by size:
2042 1478 1315 945 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 2350 3203 4362 5039 6187 6432 1606
Size: 510 234 853 1159 677 1148 245 2120
Fragments arranged by size:
2120 1159 1148 853 677 510 245 234
NaeI GCC'GGC
Cuts at: 2187 2187
Size: 6946
NcoI C'CATG_G
Cuts at: 513 1065 2004 3961 4068 513
Size: 552 939 1957 107 3391
Fragments arranged by size:
3391 1957 939 552 107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NgoMIV G'CCGG_C
Cuts at: 2185 2185
Size: 6946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 2516 4044 4304 5849 1132
Size: 97 1287 1528 260 1545 2229
Fragments arranged by size:
2229 1545 1528 1287 260 97
PacI TTA_AT'TAA
Cuts at: 3396 3396
Size: 6946
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 5845 5845
Size: 6946
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6946
PfoI T'CCnGG_A
Cuts at: 2956 2956
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 2329 2329
Size: 6946
PspXI vC'TCGA_Gb
Cuts at: 2135 2135
Size: 6946
PstI C_TGCA'G
Cuts at: 830 2328 4298 830
Size: 1498 1970 3478
Fragments arranged by size:
3478 1970 1498
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 3203 3203
Size: 6946
SacII CC_GC'GG
Cuts at: 2117 2117
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SbfI CC_TGCA'GG
Cuts at: 2328 4298 2328
Size: 1970 4976
ScaI AGT'ACT
Cuts at: 3823 5194 6906 3823
Size: 1371 1712 3863
Fragments arranged by size:
3863 1712 1371
SfcI C'TryA_G
Cuts at: 826 1045 2324 2856 4294 5429 6110 6301 826
Size: 219 1279 532 1438 1135 681 191 1471
Fragments arranged by size:
1471 1438 1279 1135 681 532 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2328 2328
Size: 6946
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 2148 2208 4273 1872
Size: 276 60 2065 4545
Fragments arranged by size:
4545 2065 276 60
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 2883 3616 5050 5951 6213
Size: 197 926 192 748 733 1434 901 262
Cuts at: 6213 820
Size: 1553
Fragments arranged by size:
1553 1434 926 901 748 733 262 197
192
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 4304 1132
Size: 3172 3774
SrfI GCCC'GGGC
Cuts at: 2148 2148
Size: 6946
SspI AAT'ATT
Cuts at: 5 52 2906 4870 5
Size: 47 2854 1964 2081
Fragments arranged by size:
2854 2081 1964 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2004 2537 3191 3961 4068 4385 513
Size: 552 939 533 654 770 107 317 3074
Fragments arranged by size:
3074 939 770 654 552 533 317 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3604 4973 4990 5143 5328 5773 325
Size: 1255 2024 1369 17 153 185 445 1498
Fragments arranged by size:
2024 1498 1369 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3748 3821 5192
Size: 274 80 33 51 1443 1771 73 1371
Cuts at: 5192 6904 96
Size: 1712 138
Fragments arranged by size:
1771 1712 1443 1371 274 138 80 73
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2778 2810 6906 6938 2778
Size: 32 4096 32 2786
Fragments arranged by size:
4096 2786 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 2135 1943
Size: 192 6754
XmaI C'CCGG_G
Cuts at: 1870 2146 2206 4271 1870
Size: 276 60 2065 4545
Fragments arranged by size:
4545 2065 276 60
XmnI GAAnn'nnTTC
Cuts at: 3267 5075 3267
Size: 1808 5138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3997 4754 276
Size: 53 83 186 936 2463 757 2468
Fragments arranged by size:
2468 2463 936 757 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI
SfoI SwaI XcmI