Restriction map for
pFN21AB5751
(Circular) (Six-base) MAPSORT of: pFN21AB5751.seq Check: 7479 from: 1 to: 7129
pFN21AB5751 7129 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2435 3238 3913 2435
Size: 803 675 5651
Fragments arranged by size:
5651 803 675
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4939 278
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4092 4472 1937
Size: 2155 380 4594
Fragments arranged by size:
4594 2155 380
Acc65I G'GTAC_C
Cuts at: 2108 3108 3228 4450 2108
Size: 1000 120 1222 4787
Fragments arranged by size:
4787 1222 1000 120
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2104 5192 6576 6844 1632
Size: 472 3088 1384 268 1917
Fragments arranged by size:
3088 1917 1384 472 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 3057 6028 7005 1203
Size: 1854 2971 977 1327
Fragments arranged by size:
2971 1854 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 5858 5858
Size: 7129
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3945 4428 1184
Size: 2761 483 3885
Fragments arranged by size:
3885 2761 483
AlwNI CAG_nnn'CTG
Cuts at: 3238 3754 3827 3844 3871 4028 4237 4872 6444
Size: 516 73 17 27 157 209 635 1572
Cuts at: 6444 3238
Size: 3923
Fragments arranged by size:
3923 1572 635 516 209 157 73 27
17
ApaI G_GGCC'C
Cuts at: 4048 4048
Size: 7129
ApaLI G'TGCA_C
Cuts at: 2951 5186 6342 2951
Size: 2235 1156 3738
Fragments arranged by size:
3738 2235 1156
ApoI r'AATT_y
Cuts at: 1493 4438 4664 4816 6925 1493
Size: 2945 226 152 2109 1697
Fragments arranged by size:
2945 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 2654 3042 4146 4454 1870
Size: 73 606 105 388 1104 308 4545
Fragments arranged by size:
4545 1104 606 388 308 105 73
BamHI G'GATC_C
Cuts at: 4459 4826 4459
Size: 367 6762
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2217 2326 2447 2668
Size: 325 217 627 321 109 109 121 221
Cuts at: 2668 3108 3228 4450 5905 618
Size: 440 120 1222 1455 1842
Fragments arranged by size:
1842 1455 1222 627 440 325 321 221
217 121 120 109 109
BanII G_rGCy'C
Cuts at: 1391 2062 2548 2729 3605 4048 4448 1391
Size: 671 486 181 876 443 400 4072
Fragments arranged by size:
4072 876 671 486 443 400 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3959 928
Size: 971 2060 4098
Fragments arranged by size:
4098 2060 971
BbvCI CC'TCA_GC
Cuts at: 4292 4292
Size: 7129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5320 5354 1903
Size: 34 3383 34 3678
Fragments arranged by size:
3678 3383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5021 6231 5021
Size: 1210 5919
BclI T'GATC_A
Cuts at: 1548 3180 1548
Size: 1632 5497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3290 5740 136
Size: 107 122 71 631 605 1618 2450 1525
Fragments arranged by size:
2450 1618 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7124 7124
Size: 7129
BlpI GC'TnA_GC
Cuts at: 2010 2842 4546 2010
Size: 832 1704 4593
Fragments arranged by size:
4593 1704 832
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2452 2673 2955 4048 4201 5190 6346
Size: 541 121 221 282 1093 153 989 1156
Cuts at: 6346 1790
Size: 2573
Fragments arranged by size:
2573 1156 1093 989 541 282 221 153
121
BmgBI CAC'GTC
Cuts at: 1318 3060 1318
Size: 1742 5387
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2501 2590
Size: 416 53 83 399 24 873 211 89
Cuts at: 2590 3648 4402 4860 5818 442
Size: 1058 754 458 958 1753
Fragments arranged by size:
1753 1058 958 873 754 458 416 399
211 89 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2193 1056
Size: 1137 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2551 3093 4276 2258
Size: 293 542 1183 5111
Fragments arranged by size:
5111 1183 542 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2927 4407 5789 1239
Size: 1688 1480 1382 2579
Fragments arranged by size:
2579 1688 1480 1382
Bpu10I CC'TnA_GC
Cuts at: 2369 4251 4292 2369
Size: 1882 41 5206
Fragments arranged by size:
5206 1882 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5254 6119 6417 5254
Size: 865 298 5966
Fragments arranged by size:
5966 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5792 882
Size: 617 4293 2219
Fragments arranged by size:
4293 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2950 4920 493
Size: 711 1746 1970 2702
Fragments arranged by size:
2702 1970 1746 711
BsaBI GATnn'nnATC
Cuts at: 7123 7123
Size: 7129
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 4936 5318 5953
Size: 53 83 186 936 639 2764 382 635
Cuts at: 5953 275
Size: 1451
Fragments arranged by size:
2764 1451 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2292 4158 5562 5960 6234 6381 6792 2292
Size: 1866 1404 398 274 147 411 2629
Fragments arranged by size:
2629 1866 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2990 3020 1178
Size: 30 165 30 1587 30 5287
Fragments arranged by size:
5287 1587 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 3347 3595 1186
Size: 1578 583 248 4720
Fragments arranged by size:
4720 1578 583 248
BseYI C'CCAG_C
Cuts at: 2080 2251 2376 2392 4100 4284 6332 6828 2080
Size: 171 125 16 1708 184 2048 496 2381
Fragments arranged by size:
2381 2048 1708 496 184 171 125 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 2919 3266 3899 4424 1163
Size: 573 825 358 347 633 525 3868
Fragments arranged by size:
3868 825 633 573 525 358 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2158 4579 5340 6368 1540
Size: 275 186 157 2421 761 1028 2301
Fragments arranged by size:
2421 2301 1028 761 275 186 157
BsiHKAI G_wGCw'C
Cuts at: 2388 2955 3821 3906 5190 5275 6346 2388
Size: 567 866 85 1284 85 1071 3171
Fragments arranged by size:
3171 1284 1071 866 567 85 85
BsmI GAATG_Cn'
Cuts at: 4640 4733 4640
Size: 93 7036
BsmBI CGTCTCn'nnnn_
Cuts at: 2726 2993 2726
Size: 267 6862
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2331 2388 2452 2548 2673 2729
Size: 399 272 269 57 64 96 125 56
Cuts at: 2729 2955 3605 3821 3906 4048 4201 4448 5190
Size: 226 650 216 85 142 153 247 742
Cuts at: 5190 5275 6346 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 399 272 269 247
226 216 153 142 125 96 85 85
64 57 56
BspEI T'CCGG_A
Cuts at: 2292 4158 6792 2292
Size: 1866 2634 2629
Fragments arranged by size:
2634 2629 1866
BspHI T'CATG_A
Cuts at: 5018 6748 5018
Size: 1730 5399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 3238 3913 3921 4484 844
Size: 1591 803 675 8 563 3489
Fragments arranged by size:
3489 1591 803 675 563 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2207 4255 4266 5016 1394
Size: 429 384 2048 11 750 3507
Fragments arranged by size:
3507 2048 750 429 384 11
BsrDI GCAATG_nn'
Cuts at: 65 1248 5624 5798 65
Size: 1183 4376 174 1396
Fragments arranged by size:
4376 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5773 5960 1700
Size: 110 3963 187 2869
Fragments arranged by size:
3963 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 5189 6201 5189
Size: 1012 6117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7129
BstBI TT'CG_AA
Cuts at: 3316 5933 3316
Size: 2617 4512
BstEII G'GTnAC_C
Cuts at: 1184 3172 3484 1184
Size: 1988 312 4829
Fragments arranged by size:
4829 1988 312
BstXI CCAn_nnnn'nTGG
Cuts at: 3217 3280 4201 3217
Size: 63 921 6145
Fragments arranged by size:
6145 921 63
BstYI r'GATC_y
Cuts at: 1972 2925 4459 4826 5211 5228 6680 6766 6778
Size: 953 1534 367 385 17 1452 86 12
Cuts at: 6778 7124 1972
Size: 346 1977
Fragments arranged by size:
1977 1534 1452 953 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2411 4071 2411
Size: 1660 5469
BtgI C'CryG_G
Cuts at: 513 1065 2004 2212 2556 2940 3168 4398 513
Size: 552 939 208 344 384 228 1230 3244
Fragments arranged by size:
3244 1230 939 552 384 344 228 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6390
Fragments arranged by size:
6390 683 56
BtsI GCAGTG_nn'
Cuts at: 2342 2624 2907 3511 3939 4140 4645 5439 5459
Size: 282 283 604 428 201 505 794 20
Cuts at: 5459 2342
Size: 4012
Fragments arranged by size:
4012 794 604 505 428 283 282 201
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7094
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DraIII CAC_nnn'GTG
Cuts at: 3656 3656
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6136 809
Size: 507 4820 1802
Fragments arranged by size:
4820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2790 3282
Size: 54 1425 50 275 18 325 635 492
Cuts at: 3282 3300 4576 5465 7094 8
Size: 18 1276 889 1629 43
Fragments arranged by size:
1629 1425 1276 889 635 492 325 275
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2155 4576 1537
Size: 275 343 2421 4090
Fragments arranged by size:
4090 2421 343 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3809 5059 1353
Size: 2456 1250 3423
Fragments arranged by size:
3423 2456 1250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2927 4407 5192 5789 6576 6844
Size: 393 472 823 1480 785 597 787 268
Cuts at: 6844 1239
Size: 1524
Fragments arranged by size:
1524 1480 823 787 785 597 472 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2993 2993
Size: 7129
EcoO109I rG'GnC_Cy
Cuts at: 2124 2397 2407 3685 3797 4044 2124
Size: 273 10 1278 112 247 5209
Fragments arranged by size:
5209 1278 273 247 112 10
EcoRI G'AATT_C
Cuts at: 4438 4816 4438
Size: 378 6751
FspI TGC'GCA
Cuts at: 5635 5635
Size: 7129
HaeII r_GCGC'y
Cuts at: 1678 3791 4341 6276 1678
Size: 2113 550 1935 2531
Fragments arranged by size:
2531 2113 1935 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2517 2549 3044 3073 3076
Size: 32 135 32 945 32 495 29 3
Cuts at: 3076 3105 3281 3313 4140 4172 4326 4358 5770
Size: 29 176 32 827 32 154 32 1412
Cuts at: 5770 5802 5844 5876 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 945 827 495 176 154 135
42 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3415 3666 4473 4719 669
Size: 988 281 1477 251 807 246 3079
Fragments arranged by size:
3079 1477 988 807 281 251 246
HindIII A'AGCT_T
Cuts at: 748 3319 748
Size: 2571 4558
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KpnI G_GTAC'C
Cuts at: 2112 3112 3232 4454 2112
Size: 1000 120 1222 4787
Fragments arranged by size:
4787 1222 1000 120
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3037 3855 6243 6427 1305
Size: 1732 818 2388 184 2007
Fragments arranged by size:
2388 2007 1732 818 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2792 3284 10
Size: 54 1425 343 960 492 3855
Fragments arranged by size:
3855 1425 960 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5087 5446 5605 518
Size: 648 3921 359 159 2042
Fragments arranged by size:
3921 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2214 2223 2376 2558 2621 3481 3868 4025
Size: 608 9 153 182 63 860 387 157
Cuts at: 4025 4111 4545 5222 6370 6615 1606
Size: 86 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 677 608 434 387 245
182 157 153 86 63 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2940 3168 4398 513
Size: 552 939 936 228 1230 3244
Fragments arranged by size:
3244 1230 939 936 552 228
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 2189 1052
Size: 1137 5992
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 4487 6032 1132
Size: 97 3258 1545 2229
Fragments arranged by size:
3258 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2452 1348
Size: 1104 6025
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3238 3556 3656 1108
Size: 2130 318 100 4581
Fragments arranged by size:
4581 2130 318 100
PfoI T'CCnGG_A
Cuts at: 3419 4179 4344 3419
Size: 760 165 6204
Fragments arranged by size:
6204 760 165
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PmlI CAC'GTG
Cuts at: 2950 2950
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 3797 2407
Size: 1390 5739
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PspOMI G'GGCC_C
Cuts at: 4044 4044
Size: 7129
PstI C_TGCA'G
Cuts at: 830 3840 3934 4033 4417 4481 830
Size: 3010 94 99 384 64 3478
Fragments arranged by size:
3478 3010 384 99 94 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 3481 3868 4025 4111 3481
Size: 387 157 86 6499
Fragments arranged by size:
6499 387 157 86
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 7129
SacII CC_GC'GG
Cuts at: 2215 2559 2215
Size: 344 6785
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SanDI GG'GwC_CC
Cuts at: 3797 3797
Size: 7129
SapI GCTCTTCn'nnn_
Cuts at: 3809 3809
Size: 7129
SbfI CC_TGCA'GG
Cuts at: 4481 4481
Size: 7129
ScaI AGT'ACT
Cuts at: 2262 5377 7089 2262
Size: 3115 1712 2302
Fragments arranged by size:
3115 2302 1712
SexAI A'CCwGG_T
Cuts at: 3111 3111
Size: 7129
SfcI C'TryA_G
Cuts at: 826 1045 3309 3746 3836 3930 4029 4413 4477
Size: 219 2264 437 90 94 99 384 64
Cuts at: 4477 5612 6293 6484 826
Size: 1135 681 191 1471
Fragments arranged by size:
2264 1471 1135 681 437 384 219 191
99 94 90 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 2551 4456 1872
Size: 679 1905 4545
Fragments arranged by size:
4545 1905 679
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5233 6134 6396 820
Size: 197 926 3290 901 262 1553
Fragments arranged by size:
3290 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4920 493
Size: 4427 2702
SpeI A'CTAG_T
Cuts at: 152 3428 152
Size: 3276 3853
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 3618 3979 5053 5
Size: 47 3566 361 1074 2081
Fragments arranged by size:
3566 2081 1074 361 47
StuI AGG'CCT
Cuts at: 1517 3123 1517
Size: 1606 5523
StyI C'CwwG_G
Cuts at: 513 1065 2004 2940 3168 4398 4568 513
Size: 552 939 936 228 1230 170 3074
Fragments arranged by size:
3074 1230 939 936 552 228 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2272 2656 2906 3563 3667 4298 5156
Size: 1255 692 384 250 657 104 631 858
Cuts at: 5156 5173 5326 5511 5956 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 858 692 657 631 445 384
250 185 153 104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 3586 4171
Size: 274 80 33 51 1443 283 1326 585
Cuts at: 4171 5375 7087 96
Size: 1204 1712 138
Fragments arranged by size:
1712 1443 1326 1204 585 283 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2685 2717 4153 4185 7089 7121 2685
Size: 32 1436 32 2904 32 2693
Fragments arranged by size:
2904 2693 1436 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2171 1316
Size: 855 6274
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 4198 2902
Size: 1296 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 2549 4454 1870
Size: 679 1905 4545
Fragments arranged by size:
4545 1905 679
XmnI GAAnn'nnTTC
Cuts at: 5258 5258
Size: 7129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4937 276
Size: 53 83 186 936 3403 2468
Fragments arranged by size:
3403 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI SacI
SfiI SfoI SrfI SwaI