Restriction map for
pFN21AB5749
(Circular) (Six-base) MAPSORT of: pFN21AB5749.seq Check: 5779 from: 1 to: 7129
pFN21AB5749 7129 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2661 4939 278
Size: 53 83 186 936 1125 2278 2468
Fragments arranged by size:
2468 2278 1125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 3369 4472 1937
Size: 643 789 1103 4594
Fragments arranged by size:
4594 1103 789 643
Acc65I G'GTAC_C
Cuts at: 2012 4450 2012
Size: 2438 4691
AclI AA'CG_TT
Cuts at: 1563 5256 5629 1563
Size: 3693 373 3063
Fragments arranged by size:
3693 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2755 4117 4332 5192 6576 6844 1632
Size: 1123 1362 215 860 1384 268 1917
Fragments arranged by size:
1917 1384 1362 1123 860 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6932
AflIII A'CryG_T
Cuts at: 1203 6028 7005 1203
Size: 4825 977 1327
Fragments arranged by size:
4825 1327 977
AgeI A'CCGG_T
Cuts at: 5960 5960
Size: 7129
AhdI GACnn_n'nnGTC
Cuts at: 3244 5858 3244
Size: 2614 4515
AleI CACnn'nnGTG
Cuts at: 1166 2498 3009 1166
Size: 1332 511 5286
Fragments arranged by size:
5286 1332 511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2974 1184
Size: 1790 5339
AlwNI CAG_nnn'CTG
Cuts at: 3067 4872 6444 3067
Size: 1805 1572 3752
Fragments arranged by size:
3752 1805 1572
ApaLI G'TGCA_C
Cuts at: 5186 6342 5186
Size: 1156 5973
ApoI r'AATT_y
Cuts at: 1493 2094 3233 4438 4664 4816 6925 1493
Size: 601 1139 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 1139 601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5683 160
Size: 872 4651 1606
Fragments arranged by size:
4651 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 4454 1870
Size: 73 1150 1361 4545
Fragments arranged by size:
4545 1361 1150 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2004 2037 2004
Size: 33 7096
BamHI G'GATC_C
Cuts at: 3472 4459 4826 3472
Size: 987 367 5775
Fragments arranged by size:
5775 987 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 4450 5905 618
Size: 325 217 627 225 2438 1455 1842
Fragments arranged by size:
2438 1842 1455 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 3122 3818 4341 4448 1391
Size: 1731 696 523 107 4072
Fragments arranged by size:
4072 1731 696 523 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2468 4088 928
Size: 971 192 377 1620 3969
Fragments arranged by size:
3969 1620 971 377 192
BbvCI CC'TCA_GC
Cuts at: 2366 2838 2366
Size: 472 6657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5320 5354 1903
Size: 34 3383 34 3678
Fragments arranged by size:
3678 3383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2933 5021 6231 2933
Size: 2088 1210 3831
Fragments arranged by size:
3831 2088 1210
BclI T'GATC_A
Cuts at: 1548 2121 1548
Size: 573 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5740 136
Size: 107 122 71 631 605 4068 1525
Fragments arranged by size:
4068 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3492 7124 3492
Size: 3632 3497
BlpI GC'TnA_GC
Cuts at: 2820 4546 2820
Size: 1726 5403
Bme1580I G_kGCm'C
Cuts at: 1790 5190 6346 1790
Size: 3400 1156 2573
Fragments arranged by size:
3400 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2335 1318
Size: 1017 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4860 5818 442
Size: 416 53 83 399 24 3443 958 1753
Fragments arranged by size:
3443 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4360 4360
Size: 7129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2497 3147 3822 3998 5789 1239
Size: 1258 650 675 176 1791 2579
Fragments arranged by size:
2579 1791 1258 675 650 176
Bpu10I CC'TnA_GC
Cuts at: 2366 2838 3477 2366
Size: 472 639 6018
Fragments arranged by size:
6018 639 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4143 5254 6119 6417 2576
Size: 1567 1111 865 298 3288
Fragments arranged by size:
3288 1567 1111 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2979 3033 4187 5792 882
Size: 617 1480 54 1154 1605 2219
Fragments arranged by size:
2219 1605 1480 1154 617 54
BsaAI yAC'GTr
Cuts at: 493 1204 2302 4920 493
Size: 711 1098 2618 2702
Fragments arranged by size:
2702 2618 1098 711
BsaBI GATnn'nnATC
Cuts at: 4389 7123 4389
Size: 2734 4395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 4936 5318 5953
Size: 53 83 186 936 1125 2278 382 635
Cuts at: 5953 275
Size: 1451
Fragments arranged by size:
2278 1451 1125 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5562 5960 6234 6381 6792 5562
Size: 398 274 147 411 5899
Fragments arranged by size:
5899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6904
Fragments arranged by size:
6904 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2306 2555 3112 4052 4160 1186
Size: 1045 75 249 557 940 108 4155
Fragments arranged by size:
4155 1045 940 557 249 108 75
BseYI C'CCAG_C
Cuts at: 2629 6332 6828 2629
Size: 3703 496 2930
Fragments arranged by size:
3703 2930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3072 3717 1163
Size: 573 1336 645 4575
Fragments arranged by size:
4575 1336 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4579 5340 6368 1540
Size: 275 186 2578 761 1028 2301
Fragments arranged by size:
2578 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3532 3818 5190 5275 6346 3532
Size: 286 1372 85 1071 4315
Fragments arranged by size:
4315 1372 1071 286 85
BsmI GAATG_Cn'
Cuts at: 3337 3701 4241 4640 4733 3337
Size: 364 540 399 93 5733
Fragments arranged by size:
5733 540 399 364 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2341 2341
Size: 7129
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3122 3532 3818 4341 4448 5190 5275
Size: 399 1332 410 286 523 107 742 85
Cuts at: 5275 6346 1391
Size: 1071 2174
Fragments arranged by size:
2174 1332 1071 742 523 410 399 286
107 85
BspEI T'CCGG_A
Cuts at: 6792 6792
Size: 7129
BspHI T'CATG_A
Cuts at: 3705 5018 6748 3705
Size: 1313 1730 4086
Fragments arranged by size:
4086 1730 1313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 3366 3927 4484 844
Size: 1328 1194 561 557 3489
Fragments arranged by size:
3489 1328 1194 561 557
BsrBI CCG'CTC
Cuts at: 1394 1823 2649 5016 1394
Size: 429 826 2367 3507
Fragments arranged by size:
3507 2367 826 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3300 3771 5624 5798 65
Size: 1183 2052 471 1853 174 1396
Fragments arranged by size:
2052 1853 1396 1183 471 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2193 2633 5773 5960 1700
Size: 110 329 54 440 3140 187 2869
Fragments arranged by size:
3140 2869 440 329 187 110 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7129
BssSI C'ACGA_G
Cuts at: 5189 6201 5189
Size: 1012 6117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7129
BstBI TT'CG_AA
Cuts at: 5933 5933
Size: 7129
BstEII G'GTnAC_C
Cuts at: 1184 2699 1184
Size: 1515 5614
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2911 3011 3202 2500
Size: 411 100 191 6427
Fragments arranged by size:
6427 411 191 100
BstYI r'GATC_y
Cuts at: 1972 3472 3492 4356 4459 4826 5211 5228 6680
Size: 1500 20 864 103 367 385 17 1452
Cuts at: 6680 6766 6778 7124 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1500 1452 864 385 367 346 103
86 20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2342 2926 3010 3195 513
Size: 552 939 12 326 584 84 185 4447
Fragments arranged by size:
4447 939 584 552 326 185 84 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3564 487
Size: 683 56 2338 4052
Fragments arranged by size:
4052 2338 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3084 3349 3425 4645 5439 5459 2440
Size: 644 265 76 1220 794 20 4110
Fragments arranged by size:
4110 1220 794 644 265 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7129
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3754 3789 529
Size: 35 3190 35 3869
Fragments arranged by size:
3869 3190 35 35
DraI TTT'AAA
Cuts at: 4433 4433
Size: 7129
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3757 4394 6136 809
Size: 507 2441 637 1742 1802
Fragments arranged by size:
2441 1802 1742 637 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2635 2901 3112
Size: 54 1425 50 275 18 805 266 211
Cuts at: 3112 3585 3726 3762 3876 4576 5465 7094 8
Size: 473 141 36 114 700 889 1629 43
Fragments arranged by size:
1629 1425 889 805 700 473 275 266
211 141 114 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 4576 1537
Size: 275 2764 4090
Fragments arranged by size:
4090 2764 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 3455 3774 4029 4061 4067 4329 4351
Size: 824 1278 319 255 32 6 262 22
Cuts at: 4351 5059 1353
Size: 708 3423
Fragments arranged by size:
3423 1278 824 708 319 262 255 32
22 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5716 6090 6236 387
Size: 1064 138 4127 374 146 1280
Fragments arranged by size:
4127 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3816 3816
Size: 7129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 2755 3147 3822 3998 4117 4332
Size: 393 865 258 392 675 176 119 215
Cuts at: 4332 5192 5789 6576 6844 1239
Size: 860 597 787 268 1524
Fragments arranged by size:
1524 865 860 787 675 597 393 392
268 258 215 176 119
EcoNI CCTnn'n_nnAGG
Cuts at: 3752 3752
Size: 7129
EcoO109I rG'GnC_Cy
Cuts at: 2626 2766 2626
Size: 140 6989
EcoRI G'AATT_C
Cuts at: 4438 4816 4438
Size: 378 6751
EcoRV GAT'ATC
Cuts at: 2887 3580 2887
Size: 693 6436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 7129
FspI TGC'GCA
Cuts at: 2168 3797 4284 5635 2168
Size: 1629 487 1351 3662
Fragments arranged by size:
3662 1629 1351 487
HaeII r_GCGC'y
Cuts at: 1678 3178 6276 1678
Size: 1500 3098 2531
Fragments arranged by size:
3098 2531 1500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3230 3262 4001 4033 5770
Size: 32 135 32 1658 32 739 32 1737
Cuts at: 5770 5802 5844 5876 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1737 1658 739 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4473 4719 669
Size: 988 281 2535 246 3079
Fragments arranged by size:
3079 2535 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7129
HpaI GTT'AAC
Cuts at: 4719 4719
Size: 7129
KpnI G_GTAC'C
Cuts at: 2016 4454 2016
Size: 2438 4691
MfeI C'AATT_G
Cuts at: 4728 4728
Size: 7129
MluI A'CGCG_T
Cuts at: 7005 7005
Size: 7129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6243 6427 1305
Size: 4938 184 2007
Fragments arranged by size:
4938 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 3587 3728 3878 10
Size: 54 1425 343 1071 684 141 150 3261
Fragments arranged by size:
3261 1425 1071 684 343 150 141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 2931 2937 3009 3722 5087 5446
Size: 648 1332 433 6 72 713 1365 359
Cuts at: 5446 5605 518
Size: 159 2042
Fragments arranged by size:
2042 1365 1332 713 648 433 359 159
72 6
MspA1I CmG'CkG
Cuts at: 1606 2236 2344 2370 2899 3339 3356 4545 5222
Size: 630 108 26 529 440 17 1189 677
Cuts at: 5222 6370 6615 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1189 1148 677 630 529 440 245
108 26 17
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 7129
NcoI C'CATG_G
Cuts at: 513 1065 2004 2926 3195 513
Size: 552 939 922 269 4447
Fragments arranged by size:
4447 939 922 552 269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7129
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 7129
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7129
NotI GC'GGCC_GC
Cuts at: 4576 4576
Size: 7129
NspI r_CATG'y
Cuts at: 1132 1229 4487 6032 1132
Size: 97 3258 1545 2229
Fragments arranged by size:
3258 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7129
PciI A'CATG_T
Cuts at: 6028 6028
Size: 7129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4213 1108
Size: 3105 4024
PfoI T'CCnGG_A
Cuts at: 2070 2607 2737 4269 2070
Size: 537 130 1532 4930
Fragments arranged by size:
4930 1532 537 130
PmeI GTTT'AAAC
Cuts at: 4433 4433
Size: 7129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5198 5230 6711 6743 5198
Size: 32 1481 32 5584
Fragments arranged by size:
5584 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2766 2766
Size: 7129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7129
PsiI TTA'TAA
Cuts at: 4699 4699
Size: 7129
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2279 2311 2279
Size: 32 7097
PstI C_TGCA'G
Cuts at: 830 3344 3924 4481 830
Size: 2514 580 557 3478
Fragments arranged by size:
3478 2514 580 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7129
PvuII CAG'CTG
Cuts at: 2370 2899 3356 2370
Size: 529 457 6143
Fragments arranged by size:
6143 529 457
SacI G_AGCT'C
Cuts at: 3818 3818
Size: 7129
SacII CC_GC'GG
Cuts at: 2345 2345
Size: 7129
SalI G'TCGA_C
Cuts at: 1936 4471 1936
Size: 2535 4594
SapI GCTCTTCn'nnn_
Cuts at: 3455 4329 3455
Size: 874 6255
SbfI CC_TGCA'GG
Cuts at: 3924 4481 3924
Size: 557 6572
ScaI AGT'ACT
Cuts at: 5377 7089 5377
Size: 1712 5417
SfcI C'TryA_G
Cuts at: 826 1045 3340 3416 3920 4477 5612 6293 6484
Size: 219 2295 76 504 557 1135 681 191
Cuts at: 6484 826
Size: 1471
Fragments arranged by size:
2295 1471 1135 681 557 504 219 191
76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7129
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7129
SmaI CCC'GGG
Cuts at: 1872 4456 1872
Size: 2584 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 4158 5233 6134 6396 820
Size: 197 926 612 1603 1075 901 262 1553
Fragments arranged by size:
1603 1553 1075 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 2302 4920 493
Size: 1809 2618 2702
Fragments arranged by size:
2702 2618 1809
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7129
SphI G_CATG'C
Cuts at: 1132 4487 1132
Size: 3355 3774
SspI AAT'ATT
Cuts at: 5 52 3025 5053 5
Size: 47 2973 2028 2081
Fragments arranged by size:
2973 2081 2028 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7129
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2880 2926 3195 3306 3561
Size: 552 939 528 348 46 269 111 255
Cuts at: 3561 4197 4568 513
Size: 636 371 3074
Fragments arranged by size:
3074 939 636 552 528 371 348 269
255 111 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3225 3644 5156 5173 5326 5511 5956
Size: 1255 1645 419 1512 17 153 185 445
Cuts at: 5956 325
Size: 1498
Fragments arranged by size:
1645 1512 1498 1255 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3714 4162 5375
Size: 274 80 33 51 1443 1737 448 1213
Cuts at: 5375 7087 96
Size: 1712 138
Fragments arranged by size:
1737 1712 1443 1213 448 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3580 3612 7089 7121 3580
Size: 32 3477 32 3588
Fragments arranged by size:
3588 3477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2939 3503 1316
Size: 1623 564 4942
Fragments arranged by size:
4942 1623 564
XbaI T'CTAG_A
Cuts at: 4465 4465
Size: 7129
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7129
XmaI C'CCGG_G
Cuts at: 1870 4454 1870
Size: 2584 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 5258 4241
Size: 1017 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2659 4937 276
Size: 53 83 186 936 1125 2278 2468
Fragments arranged by size:
2468 2278 1125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI